Inoculation Methods

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Bei-dou Xi - One of the best experts on this subject based on the ideXlab platform.

  • influences of different Inoculation Methods on fluorescence characteristics of dom in solid waste composting
    Applied Mechanics and Materials, 2013
    Co-Authors: Chun-ming Hu, Bei-dou Xi
    Abstract:

    In order to determine better Inoculation Methods of compound microorganisms in solid waste composting, biochemical parameters and dissolved organic matter (DOM) during the process of composting with five different Inoculation Methods were tested and analyzed. The results show that temperature, pH value, and synchronous fluorescence spectra of DOM in the composting piles of the five deals exhibits a certain trend with composting time increasing, in which macromolecules with complex structures and humic substances gradually increase. Overall, the temperature increases first and then decreases, and pH value is nearly stable at slightly alkaline range by the end of composting. Complex macromolecules and humic substances increase, and DOM properties tend to stabilize. Meanwhile, humification index (IA/IC, %PLF, and %HLF) of the five deals of compost also experienced significant changes. With the decrease of protein-like substances, the content of humus correspondingly increase, i.e. with composting time increasing, low-molecular-weight organic matter in the compost gradually transforms into stable high-molecular-weight humic substances, which suggests increasing aromaticity and distinctive complexity of DOM. From a comprehensive comparison of the relevant parameters and indicators, the deal of inoculating compound microorganisms at the start time or after the high-temperature phase could notably speed up the humification process in solid waste composting.

  • effect of Inoculation Methods on the composting efficiency of municipal solid wastes
    Chemosphere, 2012
    Co-Authors: Bei-dou Xi, Xiao-song He, Yonghai Jiang, Mingxiao Li, Dan Li, Ye Li, Qiuling Dang
    Abstract:

    Abstract Four types of Inoculation Methods were studied during the composting of municipal solid wastes and dry grass (MSWG). The Methods included a control group as well as initial-stage, two-stage, and multi-stage Inoculations. Fulvic acids were extracted from the composting materials and characterized by spectroscopic techniques. The results showed that Inoculation of microbes in MSWG enhanced the biodegradation of aliphatics, proteins, and polysaccharides. The Inoculation also increased the molecular weight, humic- and fulvic-like compound content, as well as humification degree of the composting products. The Inoculation of microbes in MSWG significantly improved composting process and efficiency. The improvement efficiency was in the order of initial-stage

  • Impacts of different Inoculation Methods on fluorescence characteristics of DOM in solid waste composting
    Spectroscopy and Spectral Analysis, 2011
    Co-Authors: Bei-dou Xi, Chun-ming Hu, Chun-mao Geng, Xiao-song He
    Abstract:

    : In order to determine the best Inoculation Methods of compound microorganisms in solid waste composting, dissolved organic matter (DOM) during the process of composting under five different Inoculation Methods, including Inoculation at the start, at the high-temperature phase, after the high-temperature phase, at the maturity phase and without Inoculation, was tested through spectral analysis to investigate the time variations of DOM components in solid waste composting. Fluorescence spec-tra analysis of DOM indicated that the spectral characteristics of DOM in the composting piles of the five treatments exhibited a certain trend with composting time increasing, in which macromolecules with complex structures and humic substances gradually increased, and the heap materials became more and more stabilized. According to the comparisons of the fluorescence spectroscopy maps and related parameters, the treatment of inoculating compound microorganisms at the start time or after the high-temperature phase could notably speed up the humification process in solid waste composting.

Rosane Freitas Schwan - One of the best experts on this subject based on the ideXlab platform.

  • Coffee protein profiles during fermentation using different yeast Inoculation Methods
    Pesquisa Agropecuaria Brasileira, 2020
    Co-Authors: Ana Paula Pereira Bressani, Silvia Juliana Martinez, Disney Ribeiro Dias, Leonardo De Figueiredo Vilela, Rosane Freitas Schwan
    Abstract:

    Abstract: The objective of this work was to evaluate the protein profiles of natural and semidry fermented Coffea arabica, either subjected to treatments with different yeast Inoculation Methods with starter culture or to an uninoculated control. Saccharomyces cerevisiae CCMA 0543 and Candida parapsilosis CCMA 0544 were separately inoculated into coffee by directly spraying the cherries on a terrace or in buckets, for 16 hours before sun drying. Protein quantification showed a significant difference between the protein profiles of the samples collected after natural dry fermentation. The MALDI-TOF MS analysis generated a list of 96 peaks with different mass-to-charge ratios (m/z) in the samples collected at the beginning and the end of fermentation. The highest number of peaks in the natural dry coffee was observed at the end of fermentation in the samples inoculated with S. cerevisiae CCMA 0543, in bucket, and in C. parapsilosis CCMA 0544 sprayed on the terrace. However, in the semidry processed coffee, the highest number of peaks was observed in the initial fermentation, with a decrease in the peptide peaks after fermentation. The fermentation with different microorganisms, processing types, and Inoculation Methods affects m/z profiles, influencing the types of proteins found in coffee.

  • characteristics of fermented coffee inoculated with yeast starter cultures using different Inoculation Methods
    Lwt - Food Science and Technology, 2018
    Co-Authors: Ana Paula Pereira Bressani, Silvia Juliana Martinez, Disney Ribeiro Dias, Suzana Reis Evangelista, Rosane Freitas Schwan
    Abstract:

    Abstract This study aimed to evaluate the behavior of yeasts as starter cultures for dry processed coffee. Saccharomyces cerevisiae CCMA 0543, Candida parapsilosis CCMA 0544, and Torulospora delbrueckii CCMA 0684 were separately inoculated into coffee by directly spraying the cherries on a terrace or in buckets for 16 h before sun drying. Samples were collected and analyzed by qPCR (inoculum persistence), HPLC and GC-MS. Sensory analysis was performed on the roasted coffee. All treatments directly inoculated with yeasts showed high citric and malic acid concentrations. Caffeine, chlorogenic acids and trigonelline concentrations varied between the Methods of Inoculation. A total of 217 volatile compounds were identified. After roasting, volatile compounds corresponding to caramel flavor were detected in samples inoculated with Saccharomyces cerevisiae CCMA 0543 D. Fruity flavor (apple, cherry) compounds were observed in samples inoculated with Candida parapsilosis CCMA 0544 D. Saccharomyces cerevisiae CCMA 0543 inoculated in buckets obtained the highest score in the coffee cupping. Direct Inoculation of Candida parapsilosis CCMA 0544 showed the best results when compared to the bucket method, while Torulospora delbrueckii CCMA 0684 showed good results for both Inoculation Methods evaluated.

  • different Inoculation Methods for semi dry processed coffee using yeasts as starter cultures
    Food Research International, 2017
    Co-Authors: Silvia Juliana Martinez, Ana Paula Pereira Bressani, Maria Gabriela Da Cruz Pedrozo Miguel, Disney Ribeiro Dias, Rosane Freitas Schwan
    Abstract:

    Abstract This study evaluates the behavior of Saccharomyces ( S. ) cerevisiae (CCMA 0543), Candida ( C. ) parapsilosis (CCMA 0544), and Torulaspora ( T. ) delbrueckii (CCMA 0684) as starter cultures for semi-dry processed coffee using two Inoculation Methods: (1) direct Inoculation and (2) bucket Inoculation. The microbial population was evaluated by plating and real-time polymerase chain reaction (qPCR). The microbial metabolic changes of both bucket and direct Inoculation Methods during fermentation were evaluated using high performance liquid chromatography (HPLC), and gas chromatography–mass spectrometry (GC–MS). A sensorial test was also carried out. Citric and succinic acids were detected throughout the fermentation period. Chlorogenic acid concentration levels were higher for the bucket method after roasting. Roasted coffee beans also had a higher caffeine concentration, with the exception of the T. delbrueckii (CCMA0684) assay. Acids, pyrazines and pyridines were the main volatile compounds in both green and roasted coffee beans. Coffee cupping results proved that both Inoculation Methods scored well in terms of coffee quality. The bucket method favored the permanence of the microorganisms during coffee processing, especially the treatment inoculated with S. cerevisiae .

Xiao-song He - One of the best experts on this subject based on the ideXlab platform.

  • effect of Inoculation Methods on the composting efficiency of municipal solid wastes
    Chemosphere, 2012
    Co-Authors: Bei-dou Xi, Xiao-song He, Yonghai Jiang, Mingxiao Li, Dan Li, Ye Li, Qiuling Dang
    Abstract:

    Abstract Four types of Inoculation Methods were studied during the composting of municipal solid wastes and dry grass (MSWG). The Methods included a control group as well as initial-stage, two-stage, and multi-stage Inoculations. Fulvic acids were extracted from the composting materials and characterized by spectroscopic techniques. The results showed that Inoculation of microbes in MSWG enhanced the biodegradation of aliphatics, proteins, and polysaccharides. The Inoculation also increased the molecular weight, humic- and fulvic-like compound content, as well as humification degree of the composting products. The Inoculation of microbes in MSWG significantly improved composting process and efficiency. The improvement efficiency was in the order of initial-stage

  • Impacts of different Inoculation Methods on fluorescence characteristics of DOM in solid waste composting
    Spectroscopy and Spectral Analysis, 2011
    Co-Authors: Bei-dou Xi, Chun-ming Hu, Chun-mao Geng, Xiao-song He
    Abstract:

    : In order to determine the best Inoculation Methods of compound microorganisms in solid waste composting, dissolved organic matter (DOM) during the process of composting under five different Inoculation Methods, including Inoculation at the start, at the high-temperature phase, after the high-temperature phase, at the maturity phase and without Inoculation, was tested through spectral analysis to investigate the time variations of DOM components in solid waste composting. Fluorescence spec-tra analysis of DOM indicated that the spectral characteristics of DOM in the composting piles of the five treatments exhibited a certain trend with composting time increasing, in which macromolecules with complex structures and humic substances gradually increased, and the heap materials became more and more stabilized. According to the comparisons of the fluorescence spectroscopy maps and related parameters, the treatment of inoculating compound microorganisms at the start time or after the high-temperature phase could notably speed up the humification process in solid waste composting.

Darin M Eastburn - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of Inoculation Methods for screening horseradish cultivars for resistance to verticillium dahliae
    Plant Disease, 1997
    Co-Authors: N Atibalentja, Darin M Eastburn
    Abstract:

    ABSTRACT Four Inoculation Methods—colonized oatseed, root dip, infested soil, and set dip—were tested in the greenhouse for their effectiveness in identifying horseradish cultivars that are resistant to Verticillium wilt of horseradish. Examination of the inoculum density-disease relationships derived with each of these Methods on susceptible (647A) and resistant (769A) cultivars showed that all were effective, though at varying degrees, in differentiating between susceptible and resistant reactions. Results were more consistent with the root dip method as it produced the largest least-squares means difference in wilt index between the two cultivars, the highest r 2, the lowest coefficient of variation, the shortest incubation periods, and the highest incidence of foliar symptoms. Overall, inoculum concentrations accounted for only a small amount of the total variation in wilt index (0.14 ≤ r 2 ≤ 0.73). This observation, in accord with previous reports on other hosts of Verticillium dahliae, would suggest...

Qiuling Dang - One of the best experts on this subject based on the ideXlab platform.

  • effect of Inoculation Methods on the composting efficiency of municipal solid wastes
    Chemosphere, 2012
    Co-Authors: Bei-dou Xi, Xiao-song He, Yonghai Jiang, Mingxiao Li, Dan Li, Ye Li, Qiuling Dang
    Abstract:

    Abstract Four types of Inoculation Methods were studied during the composting of municipal solid wastes and dry grass (MSWG). The Methods included a control group as well as initial-stage, two-stage, and multi-stage Inoculations. Fulvic acids were extracted from the composting materials and characterized by spectroscopic techniques. The results showed that Inoculation of microbes in MSWG enhanced the biodegradation of aliphatics, proteins, and polysaccharides. The Inoculation also increased the molecular weight, humic- and fulvic-like compound content, as well as humification degree of the composting products. The Inoculation of microbes in MSWG significantly improved composting process and efficiency. The improvement efficiency was in the order of initial-stage