The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Robin Raine - One of the best experts on this subject based on the ideXlab platform.
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an assessment of rna content in prymnesium parvum prymnesium polylepis cf chattonella sp and karlodinium veneficum under varying environmental conditions for calibrating an rna microarray for species detection
FEMS Microbiology Ecology, 2014Co-Authors: Gary R Mccoy, Jessica Kegel, Nicolas Touzet, Gerard T A Fleming, Linda K Medlin, Robin RaineAbstract:Traditional methods of identification and enumeration can be somewhat ambiguous when identifying phytoplankton that requires electron microscopic examination to verify specific morphological features. Members of the genus Prymnesium (division Haptophyta), members of the Raphidophyceae and naked dinoflagellates are examples of such phytoplankton whose identification can be difficult. One alternative to traditional microscopy-based methods of identification is to use molecular protocols to detect target species. Methods that measure cellular DNA and RNA content can be used to estimate the number of cells present in a sample. This study investigated the variation of RNA yields in Prymnesium parvum, P. polylepis, cf. Chattonella sp. and Karlodinium veneficum cells grown under different light, temperature, salinity and Inorganic Nutrient conditions. This information was used to calibrate the signal intensity of a variety of oligonucleotide probes spotted onto the microarrays for the detection of toxic algae (MIDTAL), which is being developed to aid national monitoring agencies and to provide a faster means of identifying and quantifying harmful phytoplankton in water column samples.
D N Thomas - One of the best experts on this subject based on the ideXlab platform.
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the effect of biological activity caco3 mineral dynamics and co2 degassing in the Inorganic carbon cycle in sea ice in late winter early spring in the weddell sea antarctica
Journal of Geophysical Research, 2012Co-Authors: S Papadimitriou, Hillary Kennedy, Louiza Norman, D P Kennedy, Gerhard Dieckmann, D N ThomasAbstract:A large-scale geographical study of the ice pack in the seasonal ice zone of the Weddell Sea, Antarctica, took place from September to October 2006. Sea ice brines with a salinity greater than 58 and temperature lower than -3.6oC were sampled from 22 ice stations. The brines had large deficits in total alkalinity and in the concentrations of the major dissolved macroNutrients (total dissolved Inorganic carbon, nitrate, and soluble reactive phosphorus) relative to their concentrations in the surface oceanic water and conservative behaviour during seawater freezing. The concentration deficits were related to the dissolved Inorganic carbon-consuming processes of photosynthesis, CaCO3 precipitation, and CO2 degassing. The largest concentration deficits in total dissolved Inorganic carbon were found to be associated with CaCO3 precipitation and CO2 degassing, because the magnitude of the photosynthesis-induced concentration deficit in total dissolved Inorganic carbon is controlled by the size of the Inorganic Nutrient pool, which can be limited in sea ice by its openness to exchange with the surrounding oceanic water.
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influence of freshwater inflow on the Inorganic Nutrient and dissolved organic matter within coastal sea ice and underlying waters in the gulf of finland baltic sea
Estuarine Coastal and Shelf Science, 2005Co-Authors: Mats A Granskog, D N Thomas, Hermanni Kaartokallio, Harri KuosaAbstract:Abstract A study was conducted to measure the biogeochemical characteristics of freshwater plumes underlying Baltic Sea land-fast ice, and the overlying sea ice. A 40-km long transect was conducted in the northern Baltic Sea in March 2003, following a freshwater plume from its source into the fully mixed open-sea area. The spreading of river outflow below the ice resulted in a well-stratified low-salinity surface layer further out than normally occurs in the open-water period. The freshwaters were high in dissolved organic matter (DOC, DON and CDOM), and Inorganic Nutrients (ammonium, nitrate and silicate), although the levels of phosphate were low. In general these parameters changed concurrently with salinity in such a way that mixing was conservative. The characteristics of the ice varied from the freshwater source to the open water, with increasing salinity and brine volumes (porosity) occurring in the more open-sea stations. Coinciding with the changes in ice properties there was an increase in sea-ice algal growth in the more marine stations along the transect. Biological activity in the ice was largely confined to bottom ice assemblages. In contrast to the conditions in the underlying water, no relationship between salinity, Inorganic Nutrients and organic matter was observed in the ice. In particular ammonium, phosphate, DOC and DON were present in excess of those levels predicted from the dilution curves, indicating the presence of considerable DOM production by ice assemblages, Inorganic Nutrient uptake and remineralization within the ice.
Gary R Mccoy - One of the best experts on this subject based on the ideXlab platform.
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an assessment of rna content in prymnesium parvum prymnesium polylepis cf chattonella sp and karlodinium veneficum under varying environmental conditions for calibrating an rna microarray for species detection
FEMS Microbiology Ecology, 2014Co-Authors: Gary R Mccoy, Jessica Kegel, Nicolas Touzet, Gerard T A Fleming, Linda K Medlin, Robin RaineAbstract:Traditional methods of identification and enumeration can be somewhat ambiguous when identifying phytoplankton that requires electron microscopic examination to verify specific morphological features. Members of the genus Prymnesium (division Haptophyta), members of the Raphidophyceae and naked dinoflagellates are examples of such phytoplankton whose identification can be difficult. One alternative to traditional microscopy-based methods of identification is to use molecular protocols to detect target species. Methods that measure cellular DNA and RNA content can be used to estimate the number of cells present in a sample. This study investigated the variation of RNA yields in Prymnesium parvum, P. polylepis, cf. Chattonella sp. and Karlodinium veneficum cells grown under different light, temperature, salinity and Inorganic Nutrient conditions. This information was used to calibrate the signal intensity of a variety of oligonucleotide probes spotted onto the microarrays for the detection of toxic algae (MIDTAL), which is being developed to aid national monitoring agencies and to provide a faster means of identifying and quantifying harmful phytoplankton in water column samples.
Linda K Medlin - One of the best experts on this subject based on the ideXlab platform.
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an assessment of rna content in prymnesium parvum prymnesium polylepis cf chattonella sp and karlodinium veneficum under varying environmental conditions for calibrating an rna microarray for species detection
FEMS Microbiology Ecology, 2014Co-Authors: Gary R Mccoy, Jessica Kegel, Nicolas Touzet, Gerard T A Fleming, Linda K Medlin, Robin RaineAbstract:Traditional methods of identification and enumeration can be somewhat ambiguous when identifying phytoplankton that requires electron microscopic examination to verify specific morphological features. Members of the genus Prymnesium (division Haptophyta), members of the Raphidophyceae and naked dinoflagellates are examples of such phytoplankton whose identification can be difficult. One alternative to traditional microscopy-based methods of identification is to use molecular protocols to detect target species. Methods that measure cellular DNA and RNA content can be used to estimate the number of cells present in a sample. This study investigated the variation of RNA yields in Prymnesium parvum, P. polylepis, cf. Chattonella sp. and Karlodinium veneficum cells grown under different light, temperature, salinity and Inorganic Nutrient conditions. This information was used to calibrate the signal intensity of a variety of oligonucleotide probes spotted onto the microarrays for the detection of toxic algae (MIDTAL), which is being developed to aid national monitoring agencies and to provide a faster means of identifying and quantifying harmful phytoplankton in water column samples.
Nicolas Touzet - One of the best experts on this subject based on the ideXlab platform.
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an assessment of rna content in prymnesium parvum prymnesium polylepis cf chattonella sp and karlodinium veneficum under varying environmental conditions for calibrating an rna microarray for species detection
FEMS Microbiology Ecology, 2014Co-Authors: Gary R Mccoy, Jessica Kegel, Nicolas Touzet, Gerard T A Fleming, Linda K Medlin, Robin RaineAbstract:Traditional methods of identification and enumeration can be somewhat ambiguous when identifying phytoplankton that requires electron microscopic examination to verify specific morphological features. Members of the genus Prymnesium (division Haptophyta), members of the Raphidophyceae and naked dinoflagellates are examples of such phytoplankton whose identification can be difficult. One alternative to traditional microscopy-based methods of identification is to use molecular protocols to detect target species. Methods that measure cellular DNA and RNA content can be used to estimate the number of cells present in a sample. This study investigated the variation of RNA yields in Prymnesium parvum, P. polylepis, cf. Chattonella sp. and Karlodinium veneficum cells grown under different light, temperature, salinity and Inorganic Nutrient conditions. This information was used to calibrate the signal intensity of a variety of oligonucleotide probes spotted onto the microarrays for the detection of toxic algae (MIDTAL), which is being developed to aid national monitoring agencies and to provide a faster means of identifying and quantifying harmful phytoplankton in water column samples.