The Experts below are selected from a list of 1257 Experts worldwide ranked by ideXlab platform
Yu-guo Zheng - One of the best experts on this subject based on the ideXlab platform.
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enzymatic production of 5 Inosinic Acid by a newly synthesised Acid phosphatase phosphotransferase
Food Chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
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Enzymatic production of 5′-Inosinic Acid by a newly synthesised Acid phosphatase/phosphotransferase
Food chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
Zhi-qiang Liu - One of the best experts on this subject based on the ideXlab platform.
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enzymatic production of 5 Inosinic Acid by a newly synthesised Acid phosphatase phosphotransferase
Food Chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
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Enzymatic production of 5′-Inosinic Acid by a newly synthesised Acid phosphatase/phosphotransferase
Food chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
Ling Zhang - One of the best experts on this subject based on the ideXlab platform.
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enzymatic production of 5 Inosinic Acid by a newly synthesised Acid phosphatase phosphotransferase
Food Chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
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Enzymatic production of 5′-Inosinic Acid by a newly synthesised Acid phosphatase/phosphotransferase
Food chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
Nan-wei Wan - One of the best experts on this subject based on the ideXlab platform.
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enzymatic production of 5 Inosinic Acid by a newly synthesised Acid phosphatase phosphotransferase
Food Chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
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Enzymatic production of 5′-Inosinic Acid by a newly synthesised Acid phosphatase/phosphotransferase
Food chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
Li-hui Sun - One of the best experts on this subject based on the ideXlab platform.
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enzymatic production of 5 Inosinic Acid by a newly synthesised Acid phosphatase phosphotransferase
Food Chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.
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Enzymatic production of 5′-Inosinic Acid by a newly synthesised Acid phosphatase/phosphotransferase
Food chemistry, 2012Co-Authors: Zhi-qiang Liu, Ling Zhang, Li-hui Sun, Nan-wei Wan, Yu-guo ZhengAbstract:Abstract 5′-Nucleotides including 5′-Inosinic Acid have characteristic taste and important application in various foods as flavour potentiators. The selective nucleoside Acid phosphatase/phosphotransferase (AP/PTase) can catalyse the synthesis of 5′-nucleotides by transfer of phosphate groups. In this study, a 747-bp gene encoding AP/PTase from Escherichia blattae was synthesised. After expression, the recombinant AP/PTase was purified using nickel–NTA. The optimal temperature and pH of this enzyme were 30 °C and 5.0, respectively. The activity was partially inhibited by metal ions such as Hg 2+ , Ag + and Cu 2+ , but not by chelating reagents such as EDTA. The values of K m and V max for inosine were 40 mM and 3.5 U/mg, respectively. Using this purified enzyme, 16.83 mM of 5′-IMP was synthesised from 37 mM of inosine and the molar yield reached 45.5%. Homology modelling and docking simulation were discussed.