The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Yanghu Sima - One of the best experts on this subject based on the ideXlab platform.
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chitin synthase a a novel epidermal development regulation gene in the larvae of bombyx mori
Molecular Biology Reports, 2014Co-Authors: Weiwei Zhuo, Lingfei Kong, Yan Fang, Yanghu SimaAbstract:Chitin synthase is the key regulatory enzyme for chitin synthesis and excretion in insects, as well as a specific target of insecticides. The chitin synthase A gene (BmChsA) cloned from Bombyx mori, the model species of lepidopteran, is an epidermis-specific expressed gene during the molting stage. Knockdown BmChsA gene in 3rd instar larvae increased the number of non-molting and abnormal molting larvae. Exposure to nikkomycin Z, a chitin synthase inhibitor downregulated the expression of BmChsA and decreased the amount of epidermis chitin during the molting Process. The thickness of the new epidermis and its dense structure varied greatly. The exogenous hormones significantly upregulated the expression of BmChsA with low levels of endogenous MH and high levels of endogenous JH immediately after molting. With low levels of endogenous hormones during the mulberry Intake Process, BmChsA was rarely upregulated by exogenous hormones. With high levels of endogenous MH and low levels of endogenous JH during the molting stage, we did not detect the upregulation of BmChsA by exogenous hormones. The expression of BmChsA was regulated by endocrine hormones, which directly affected the chitin synthesis-dependent epidermal regeneration and molting Process.
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chitin synthase b a midgut specific gene induced by insect hormones and involved in food Intake in bombyx mori larvae
Archives of Insect Biochemistry and Physiology, 2014Co-Authors: Weiwei Zhuo, Feng Chu, Lingfei Kong, Hui Tao, Yanghu SimaAbstract:Chitin synthase (CHS) is the key regulatory enzyme in chitin synthesis and excretion in insects, and a specific target of insecticides. We cloned a CHS B gene of Bombyx mori (BmChsB) and showed it to be midgut specific, highly expressed during the feeding Process in the larva. Knockdown of BmChsB expression in the third-instar larvae increased the number of nonmolting and abnormally molting larvae. Exposure to nikkomycin Z, a CHS inhibitor, reduced the amount of chitin in the peritrophic membrane of molted larvae, whereas abnormally elevated BmChsB mRNA levels were readily detected from the end of molting and in the newly molted larvae. Exogenous 20-hydroxyecdysone (20E) and methoprene, a juvenile hormone analogue, significantly upregulated the expression of BmChsB when the levels of endogenous molting hormone (MH) were low and the levels of endogenous juvenile hormone (JH) were high immediately after molting. When levels of endogenous MH were high and those of endogenous JH were low during the molting stage, exogenous 20E did not upregulate BmChsB expression and exogenous methoprene upregulated it negligibly. When the endogenous hormone levels were low during the mulberry-leaf Intake Process, BmChsB expression was upregulated by exogenous methoprene. We conclude that the expression of BmChsB is regulated by insect hormones, and directly affects the chitin-synthesis-dependent form of the peritrophic membrane and protects the food Intake and molting Process of silkworm larvae.
L Esserman - One of the best experts on this subject based on the ideXlab platform.
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abstract p2 15 02 web based patient Intake Process for breast care center the patient experience
Cancer Research, 2010Co-Authors: Es Hwang, M Melisko, M Mendelsohn, Hope S Rugo, S Schwab, Deborah Hamolsky, Cheryl Ewing, Michael Alvarado, Jong Heum Park, L EssermanAbstract:Background: An important driver of health information technology will be an increased incorporation of self-reported patient health data into patient care workflow. We undertook this study to determine the feasibility, satisfaction, and challenges reported by patients related to the introduction of an electronic patient Intake tool in an outpatient breast care clinic. Methods: A web-based self-reported new patient Intake Process was implemented in 2008 at an urban multi-disciplinary breast care clinic. The system was based on a platform developed by Dynamic Clinical Systems (DCS) which was designed to collect, warehouse, and provide reporting of data to inform patient treatment decisions. Patients new to the Breast Care Center with either breast symptoms or a newly diagnosed breast cancer were provided a weblink for data entry prior to the clinic visit. Patients were asked questions regarding medical/family history, symptoms, and psychosocial well-being. Data regarding the survey were compared among age groups using the chi square test. Results: The first full calendar year for which data were collected was 1/1/09-12/31/09 during which 1218 women responded to the survey. Data were evaluated among 4 age groups: 0-18 (n=9), 19-49 (n=508), 50-69 (n=586), and ≥70 (n=115). 400 women (36%) were newly diagnosed with breast cancer; 106 (10%) of respondents had stage IV breast cancer. Overall 94.3% of patients took the survey themselves; 87% took the survey at home, compared to 7% in the doctors’ office. 89% reported using a high-speed (DSL, cable, wireless) internet connection. 69% of respondents completed the survey in less than one hour. Patients who completed the survey in the clinic (n=55) cited lack of access to the internet as the most common reason the survey was not completed in advance of the clinic visit (n=21). 64% of patients indicated e-mail as their preferred contact method; fewer women over 70 preferred e-mail contact to phone compared to younger groups (43%; P Conclusion: Assessment of patients’ experience using a self-reported web-based patient Intake form was conducted during the first full year of implementation. Most patients successfully completed the survey and indicated a high satisfaction level with its use. However, some differences were seen between age groups. Overall, Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P2-15-02.
Weiwei Zhuo - One of the best experts on this subject based on the ideXlab platform.
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chitin synthase a a novel epidermal development regulation gene in the larvae of bombyx mori
Molecular Biology Reports, 2014Co-Authors: Weiwei Zhuo, Lingfei Kong, Yan Fang, Yanghu SimaAbstract:Chitin synthase is the key regulatory enzyme for chitin synthesis and excretion in insects, as well as a specific target of insecticides. The chitin synthase A gene (BmChsA) cloned from Bombyx mori, the model species of lepidopteran, is an epidermis-specific expressed gene during the molting stage. Knockdown BmChsA gene in 3rd instar larvae increased the number of non-molting and abnormal molting larvae. Exposure to nikkomycin Z, a chitin synthase inhibitor downregulated the expression of BmChsA and decreased the amount of epidermis chitin during the molting Process. The thickness of the new epidermis and its dense structure varied greatly. The exogenous hormones significantly upregulated the expression of BmChsA with low levels of endogenous MH and high levels of endogenous JH immediately after molting. With low levels of endogenous hormones during the mulberry Intake Process, BmChsA was rarely upregulated by exogenous hormones. With high levels of endogenous MH and low levels of endogenous JH during the molting stage, we did not detect the upregulation of BmChsA by exogenous hormones. The expression of BmChsA was regulated by endocrine hormones, which directly affected the chitin synthesis-dependent epidermal regeneration and molting Process.
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chitin synthase b a midgut specific gene induced by insect hormones and involved in food Intake in bombyx mori larvae
Archives of Insect Biochemistry and Physiology, 2014Co-Authors: Weiwei Zhuo, Feng Chu, Lingfei Kong, Hui Tao, Yanghu SimaAbstract:Chitin synthase (CHS) is the key regulatory enzyme in chitin synthesis and excretion in insects, and a specific target of insecticides. We cloned a CHS B gene of Bombyx mori (BmChsB) and showed it to be midgut specific, highly expressed during the feeding Process in the larva. Knockdown of BmChsB expression in the third-instar larvae increased the number of nonmolting and abnormally molting larvae. Exposure to nikkomycin Z, a CHS inhibitor, reduced the amount of chitin in the peritrophic membrane of molted larvae, whereas abnormally elevated BmChsB mRNA levels were readily detected from the end of molting and in the newly molted larvae. Exogenous 20-hydroxyecdysone (20E) and methoprene, a juvenile hormone analogue, significantly upregulated the expression of BmChsB when the levels of endogenous molting hormone (MH) were low and the levels of endogenous juvenile hormone (JH) were high immediately after molting. When levels of endogenous MH were high and those of endogenous JH were low during the molting stage, exogenous 20E did not upregulate BmChsB expression and exogenous methoprene upregulated it negligibly. When the endogenous hormone levels were low during the mulberry-leaf Intake Process, BmChsB expression was upregulated by exogenous methoprene. We conclude that the expression of BmChsB is regulated by insect hormones, and directly affects the chitin-synthesis-dependent form of the peritrophic membrane and protects the food Intake and molting Process of silkworm larvae.
Zhijun Peng - One of the best experts on this subject based on the ideXlab platform.
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effects of variable valve lift on in cylinder air motion
Energies, 2015Co-Authors: Tianyou Wang, Daming Liu, Gangde Wang, Bingqian Tan, Zhijun PengAbstract:An investigation into in-cylinder swirl and tumble flow characteristics with reduced maximum valve lifts (MVL) is presented. The experimental work was conducted in the modified four-valve optical spark-ignition (SI) test engine with three different MVL. Particle image velocimetry (PIV) was employed for measuring in-cylinder air motion and measurement results were analyzed for examining flow field, swirl and tumble ratio variation and fluctuating kinetic energy distribution. Results of ensemble-averaged flow fields show that reduced MVL could produce strong swirl flow velocity, then resulted in very regular swirl motion in the late stage of the Intake Process. The strong swirl flow can maintain very well until the late compression stage. The reduction of MVL can also increase both high-frequency and low-frequency swirl flow fluctuating kinetic energy remarkably. Regarding tumble flow, results demonstrate that lower MVLs result in more horizontal Intake flow velocity vectors which can be easily detected under the valve seat area. Although the result of lower MVLs show a higher tumble ratio when the piston is close to the bottom dead centre (BDC), higher MVLs substantially produce higher tumble ratios which can be confirmed when most cylinder area lies in the measuring range.
Lingfei Kong - One of the best experts on this subject based on the ideXlab platform.
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chitin synthase a a novel epidermal development regulation gene in the larvae of bombyx mori
Molecular Biology Reports, 2014Co-Authors: Weiwei Zhuo, Lingfei Kong, Yan Fang, Yanghu SimaAbstract:Chitin synthase is the key regulatory enzyme for chitin synthesis and excretion in insects, as well as a specific target of insecticides. The chitin synthase A gene (BmChsA) cloned from Bombyx mori, the model species of lepidopteran, is an epidermis-specific expressed gene during the molting stage. Knockdown BmChsA gene in 3rd instar larvae increased the number of non-molting and abnormal molting larvae. Exposure to nikkomycin Z, a chitin synthase inhibitor downregulated the expression of BmChsA and decreased the amount of epidermis chitin during the molting Process. The thickness of the new epidermis and its dense structure varied greatly. The exogenous hormones significantly upregulated the expression of BmChsA with low levels of endogenous MH and high levels of endogenous JH immediately after molting. With low levels of endogenous hormones during the mulberry Intake Process, BmChsA was rarely upregulated by exogenous hormones. With high levels of endogenous MH and low levels of endogenous JH during the molting stage, we did not detect the upregulation of BmChsA by exogenous hormones. The expression of BmChsA was regulated by endocrine hormones, which directly affected the chitin synthesis-dependent epidermal regeneration and molting Process.
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chitin synthase b a midgut specific gene induced by insect hormones and involved in food Intake in bombyx mori larvae
Archives of Insect Biochemistry and Physiology, 2014Co-Authors: Weiwei Zhuo, Feng Chu, Lingfei Kong, Hui Tao, Yanghu SimaAbstract:Chitin synthase (CHS) is the key regulatory enzyme in chitin synthesis and excretion in insects, and a specific target of insecticides. We cloned a CHS B gene of Bombyx mori (BmChsB) and showed it to be midgut specific, highly expressed during the feeding Process in the larva. Knockdown of BmChsB expression in the third-instar larvae increased the number of nonmolting and abnormally molting larvae. Exposure to nikkomycin Z, a CHS inhibitor, reduced the amount of chitin in the peritrophic membrane of molted larvae, whereas abnormally elevated BmChsB mRNA levels were readily detected from the end of molting and in the newly molted larvae. Exogenous 20-hydroxyecdysone (20E) and methoprene, a juvenile hormone analogue, significantly upregulated the expression of BmChsB when the levels of endogenous molting hormone (MH) were low and the levels of endogenous juvenile hormone (JH) were high immediately after molting. When levels of endogenous MH were high and those of endogenous JH were low during the molting stage, exogenous 20E did not upregulate BmChsB expression and exogenous methoprene upregulated it negligibly. When the endogenous hormone levels were low during the mulberry-leaf Intake Process, BmChsB expression was upregulated by exogenous methoprene. We conclude that the expression of BmChsB is regulated by insect hormones, and directly affects the chitin-synthesis-dependent form of the peritrophic membrane and protects the food Intake and molting Process of silkworm larvae.