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Lorraine B Ware - One of the best experts on this subject based on the ideXlab platform.
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soluble Intercellular Adhesion Molecule 1 and clinical outcomes in patients with acute lung injury
Intensive Care Medicine, 2009Co-Authors: Carolyn S Calfee, Mark D Eisner, Polly E Parsons, Taylor B Thompson, Edward R Conner, Michael A Matthay, Lorraine B WareAbstract:Objective To determine if levels of soluble Intercellular Adhesion Molecule-1 (sICAM-1), a marker of alveolar epithelial and endothelial injury, differ in patients with hydrostatic pulmonary edema and acute lung injury (ALI) and are associated with clinical outcomes in patients with ALI.
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plasma Intercellular Adhesion Molecule 1 and von willebrand factor in primary graft dysfunction after lung transplantation
American Journal of Transplantation, 2007Co-Authors: M Covarrubias, Lorraine B Ware, Steven M Kawut, J De Andrade, Aaron P Milstone, Ann Weinacker, Jonathan B Orens, Vibha N Lama, Keith M Wille, Scarlett L BellamyAbstract:Primary graft dysfunction (PGD), a form of acute lung injury occurring within 72 h following lung transplantation, is characterized by pulmonary edema and diffuse alveolar damage. We hypothesized that higher concentrations of Intercellular Adhesion Molecule-1 (ICAM-1) and von Willebrand factor (vWF) would be associated with the occurrence of PGD. A total of 128 lung transplant recipients among 7 lung transplant centers were enrolled in a multicenter, prospective, cohort study. Blood specimens were collected preoperatively and at 6, 24, 48 and 72 h following lung transplantation. The primary outcome was Grade 3 PGD at 72 h after transplant. Logistic regression and generalized estimating equations (GEE) were used to analyze plasma ICAM-1 and vWF. At each postoperative timepoint, mean plasma ICAM-1 concentrations were higher for patients with PGD versus no PGD. The GEE contrast estimate for the association of plasma ICAM-1 with PGD was 107.5 ng/mL (95% CI 38.7, 176.3), p = 0.002. In the multivariate analyses, this finding was independent of all clinical variables except pulmonary artery pressures prior to transplant. There was no association between plasma vWF levels and PGD. We conclude that higher levels of plasma ICAM-1 are associated with PGD following lung transplantation.
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early elevation of plasma soluble Intercellular Adhesion Molecule 1 in pediatric acute lung injury identifies patients at increased risk of death and prolonged mechanical ventilation
Pediatric Critical Care Medicine, 2003Co-Authors: Heidi R Flori, Lorraine B Ware, David V Glidden, Michael A MatthayAbstract:ObjectiveTo determine whether soluble Intercellular Adhesion Molecule (sICAM)-1, a biological marker of alveolar epithelial and lung endothelial injury and alveolar macrophage activation, is elevated in the plasma of pediatric patients with acute lung injury and to examine whether elevated plasma sI
Robert Rothlein - One of the best experts on this subject based on the ideXlab platform.
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alterations in circulating Intercellular Adhesion Molecule 1 and l selectin further evidence for chronic inflammation in ischemic heart disease
American Heart Journal, 1996Co-Authors: Robert Rothlein, Elizabeth Mainolfi, Herbert W Haught, Michael Mansour, Takashi Kei KishimotoAbstract:Abstract Atherosclerosis is increasingly thought to be a chronic inflammatory disease. Inflammation requires transmigration of leukocytes from the circulation to the tissues. Adhesion of leukocytes to endothelial cells is the initial event in an inflammatory response and is mediated by expression of several Adhesion Molecules. In this study we characterize the contribution of Intercellular Adhesion Molecule-1 (ICAM-1) and L-selectin in patients with different coronary artery disease syndromes. Serum concentrations of cICAM-1 and sL-selectin were measured by enzyme-linked immunosorbent assay in 31 patients with stable angina, 30 patients with unstable angina, 18 patients with acute myocardial infarction and 20 healthy subjects in a control group. All patients underwent coronary angiography. Mean (±SE) cICAM-1 levels were higher ( p p
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treatment of refractory rheumatoid arthritis with a monoclonal antibody to Intercellular Adhesion Molecule 1
Arthritis & Rheumatism, 1994Co-Authors: Arthur F Kavanaugh, Robert Rothlein, Laurie S Davis, Lisa A Nichols, Stephen Norris, Linda Scharschmidt, Peter E LipskyAbstract:Objective. To assess the safety and efficacy of a monoclonal antibody (MAb) to Intercellular Adhesion Molecule 1 (ICAM-1; CD54) in rheumatoid arthritis (RA). Methods. A phase I/II, open-label, dose-escalation study of 32 patients. Results. During treatment, a peripheral CD3+/CD4+ lymphocytosis was noted, and several patients demonstrated transient cutaneous anergy, which suggests that therapy modified T cell recirculation. Thirteen of the 23 patients who received 5 days of treatment demonstrated clinical improvement through day 29, and 9 of 23 through day 60. Adverse effects were minor and transient. Conclusion. Anti—ICAM-1 MAb therapy was well tolerated, resulted in a transient alteration in T lymphocyte recirculation, and effected clinical improvement in some RA patients.
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circulating Intercellular Adhesion Molecule 1 in amniotic fluid maternal serum α fetoprotein levels and intrauterine growth retardation
American Journal of Obstetrics and Gynecology, 1993Co-Authors: Carolyn Salafia, Greggory R Devore, Elizabeth Mainolfi, Joann C Kelly, John C Pezzullo, Robert RothleinAbstract:Objective: Our purpose was to determine if circulating Intercellular Adhesion Molecule-1, a marker of chronic inflammation, is present in amniotic fluid in midtrimester, is increased in patients with elevated maternal serum α-fetoprotein level, and is associated with intrauterine growth retardation. Study Design: Amniotic fluid circulating Intercellular Adhesion Molecule-1 levels were assayed by enzyme-linked immunoassay in 273 samples obtained by midtrimester amniocentesis in gestations involving a single, structurally normal fetus. The control group consisted of 108 patients with normal maternal serum α-fetoprotein levels and 165 patients with elevated levels. Intrauterine growth retardation was diagnosed if birth weight was Results: Circulating Intercellular Adhesion Molecule-1 was detectable in amniotic fluid in 105 of 273 samples (38%). In the control group it was detectable in amniotic fluid in seven of 108 (6%). In patients with elevated maternal serum α-fetoprotein 97 of 164 (59%) had detectable levels ( p p p Conclusions: Midtrimester amniotic fluid from normal pregnancies does not generally contain detectable circulating Intercellular Adhesion Molecule-1. Detectable amniotic fluid levels are significantly related to a birth weight
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reduction of central nervous system ischemic injury by monoclonal antibody to Intercellular Adhesion Molecule
Journal of Neurosurgery, 1991Co-Authors: Wayne M Clark, Robert Rothlein, Ken P Madden, Justin A ZivinAbstract:Activated leukocytes appear to be directly involved in potentiating ischemic central nervous system (CNS) injury. Adhesion of leukocytes to endothelium is essential for their migration and requires the binding of Adhesion receptors of the leukocyte (CD 18) to an Intercellular Adhesion Molecule (ICAM) on endothelium. Monoclonal antibodies to an ICAM can block leukocyte Adhesion and transendothelial migration. To determine the efficacy of anti-ICAM antibody treatment in preserving neurological function after CNS ischemia, two animal models were used. A 1-mg/kg dose of anti-ICAM was given to rabbits 30 minutes before induction of ischemia either in the spinal cord using temporary aortic occlusion or in the brain using intra-arterial microspheres. In this study, treatment with anti-ICAM produced a significant reduction in neurological deficits in the reversible spinal cord ischemia model but not in the irreversible brain ischemia model. This protective effect supports the active role of leukocytes in CNS reperfusion ischemic injury and offers potential for future therapy.
William R Wagner - One of the best experts on this subject based on the ideXlab platform.
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ultrasound imaging of acute cardiac transplant rejection with microbubbles targeted to Intercellular Adhesion Molecule 1
Circulation, 2003Co-Authors: Gregory E R Weller, William R Wagner, Melissa Csikari, Alexander L Klibanov, David Fischer, Flordeliza S VillanuevaAbstract:Background— Noninvasive techniques for detecting acute cardiac transplant rejection are limited. We hypothesized that ultrasound contrast microbubbles targeted to the endothelial cell (EC) inflammatory marker Intercellular Adhesion Molecule-1 (ICAM-1) would selectively bind to rejecting versus nonrejecting myocardium and that myocardial contrast echocardiography can therefore detect acute rejection. Methods and Results— Lipid-based microbubbles were conjugated to anti-rat ICAM-1 (MBICAM) or isotype control antibody (MBControl). In vitro MBICAM Adhesion to cultured rat ECs, as assessed in a parallel plate flow apparatus, was greater to inflammatory versus normal ECs (11±3 versus 3±2 microbubbles/EC, P<0.005). In vivo abdominal heterotopic heart transplantation was performed in rats (rejection group: Brown Norway to Lewis strain; control group: Lewis to Lewis or Brown Norway to Brown Norway). Triggered myocardial contrast echocardiography was performed during intravenous MBICAM or MBControl (2.5×106) inject...
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microbubbles targeted to Intercellular Adhesion Molecule 1 bind to activated coronary artery endothelial cells
Circulation, 1998Co-Authors: Flordeliza S Villanueva, Ron J Jankowski, Sasha Klibanov, Maris L Pina, Sean Alber, Simon C Watkins, Gary H Brandenburger, William R WagnerAbstract:Background—Preclinical atherosclerosis is associated with increased endothelial cell (EC) expression of leukocyte Adhesion Molecules (LAMs), which mediate monocyte Adhesion during atherogenesis. Identification of cell-surface LAMs may uniquely allow assessment of endothelial function, but there are no in vivo methods for detecting LAMs. We tested a new microbubble designed to bind to and allow specific ultrasound detection of Intercellular Adhesion Molecule-1 (ICAM-1). Methods and Results—A perfluorobutane gas–filled lipid-derived microsphere with monoclonal antibody to ICAM-1 covalently bound to the bubble shell was synthesized. Bubbles with either nonspecific IgG or no protein on the shell were synthesized as controls. Coverslips of cultured human coronary artery ECs were placed in a parallel-plate perfusion chamber and exposed to 1 of the 3 microbubble species, followed by perfusion with culture medium. Experiments were performed with either normal or interleukin-1β–activated ECs overexpressing ICAM-1,...
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microbubbles targeted to Intercellular Adhesion Molecule 1 bind to activated coronary artery endothelial cells
Circulation, 1998Co-Authors: Flordeliza S Villanueva, Ron J Jankowski, Sasha Klibanov, Maris L Pina, Sean Alber, Simon C Watkins, Gary H Brandenburger, William R WagnerAbstract:BACKGROUND: Preclinical atherosclerosis is associated with increased endothelial cell (EC) expression of leukocyte Adhesion Molecules (LAMs), which mediate monocyte Adhesion during atherogenesis. Identification of cell-surface LAMs may uniquely allow assessment of endothelial function, but there are no in vivo methods for detecting LAMs. We tested a new microbubble designed to bind to and allow specific ultrasound detection of Intercellular Adhesion Molecule-1 (ICAM-1). METHODS AND RESULTS: A perfluorobutane gas-filled lipid-derived microsphere with monoclonal antibody to ICAM-1 covalently bound to the bubble shell was synthesized. Bubbles with either nonspecific IgG or no protein on the shell were synthesized as controls. Coverslips of cultured human coronary artery ECs were placed in a parallel-plate perfusion chamber and exposed to 1 of the 3 microbubble species, followed by perfusion with culture medium. Experiments were performed with either normal or interleukin-1beta-activated ECs overexpressing ICAM-1, and bubble adherence was quantified with epifluorescent videomicroscopy. There was limited adherence of control bubbles to normal or activated ECs, whereas a 40-fold increase in Adhesion occurred when anti-ICAM-1-conjugated bubbles were exposed to activated ECs compared with normal ECs (8.1+/-3.5 versus 0.21+/-0.09 bubbles per cell, respectively, P<0.001). Although diminished, this difference persisted even after perfusion at higher wall shear rates. CONCLUSIONS: A gas-filled microbubble with anti-ICAM-1 antibody on its shell specifically binds to activated ECs overexpressing ICAM-1. Diagnostic ultrasound in conjunction with targeted contrast agents has the unique potential to characterize cell phenotype in vivo.
Michael A Matthay - One of the best experts on this subject based on the ideXlab platform.
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soluble Intercellular Adhesion Molecule 1 and clinical outcomes in patients with acute lung injury
Intensive Care Medicine, 2009Co-Authors: Carolyn S Calfee, Mark D Eisner, Polly E Parsons, Taylor B Thompson, Edward R Conner, Michael A Matthay, Lorraine B WareAbstract:Objective To determine if levels of soluble Intercellular Adhesion Molecule-1 (sICAM-1), a marker of alveolar epithelial and endothelial injury, differ in patients with hydrostatic pulmonary edema and acute lung injury (ALI) and are associated with clinical outcomes in patients with ALI.
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early elevation of plasma soluble Intercellular Adhesion Molecule 1 in pediatric acute lung injury identifies patients at increased risk of death and prolonged mechanical ventilation
Pediatric Critical Care Medicine, 2003Co-Authors: Heidi R Flori, Lorraine B Ware, David V Glidden, Michael A MatthayAbstract:ObjectiveTo determine whether soluble Intercellular Adhesion Molecule (sICAM)-1, a biological marker of alveolar epithelial and lung endothelial injury and alveolar macrophage activation, is elevated in the plasma of pediatric patients with acute lung injury and to examine whether elevated plasma sI
David R Jacobs - One of the best experts on this subject based on the ideXlab platform.
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polymorphisms in the icam1 gene predict circulating soluble Intercellular Adhesion Molecule 1 sicam 1
Atherosclerosis, 2011Co-Authors: Suzette J Bielinski, Alexander P Reiner, Deborah A Nickerson, Christopher S Carlson, Kent R Bailey, Bharat Thyagarajan, Leslie A Lange, Eric Boerwinkle, David R JacobsAbstract:Abstract Objective Polymorphisms within the ICAM1 structural gene have been shown to influence circulating levels of soluble Intercellular Adhesion Molecule-1 (sICAM-1) but their relation to atherosclerosis has not been clearly established. We sought to determine whether ICAM1 SNPs are associated with circulating sICAM-1 concentration, coronary artery calcium (CAC), and common and internal carotid intima medial thickness (IMT). Methods and Results 3550 black and white Coronary Artery Risk Development in Young Adults (CARDIA) Study subjects who participated in the year 15 and/or 20 examinations and were part of the Young Adult Longitudinal Study of Antioxidants (YALTA) ancillary study were included in this analysis. In whites, rs5498 was significantly associated with sICAM-1 ( p ICAM1 SNPs. Conclusions In CARDIA, ICAM1 DNA segment variants were associated with sICAM-1 protein level including the novel finding that levels differ by the functional variant rs5491. However, ICAM1 SNPs were not strongly related to either IMT or CAC. Our findings in CARDIA suggest that ICAM1 variants are not major early contributors to subclinical atherosclerosis.