The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Yulong Yin - One of the best experts on this subject based on the ideXlab platform.
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Developmental changes in Intercellular Junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods
Journal of Animal Science and Biotechnology, 2016Co-Authors: Jing Wang, Bie Tan, Guangran Li, Liming Zeng, Xia Xiong, Fengna Li, Xiangfeng Kong, Bo Huang, Gang Liu, Yulong YinAbstract:BackgroundThe intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different Intercellular Junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, Intercellular Junctions and voltage-gated potassium (Kv) channels in the intestine of piglets during the suckling and post-weaning periods.ResultsSamples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen (PCNA)-positive cells and alkaline phosphatase (AKP) activity, as well as the abundances of E-cadherin, occludin, and Kv1.5 mRNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1 to d 21 during the suckling period (P
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Developmental changes in Intercellular Junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods.
Journal of animal science and biotechnology, 2016Co-Authors: Jing Wang, Bie Tan, Liming Zeng, Xia Xiong, Xiangfeng Kong, Bo Huang, Gang Liu, Yulong YinAbstract:Background The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different Intercellular Junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, Intercellular Junctions and voltage-gated potassium (Kv) channels in the intestine of piglets during the suckling and post-weaning periods.
Wayne A Vogl - One of the best experts on this subject based on the ideXlab platform.
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internalization of intact Intercellular Junctions in the testis by clathrin actin mediated endocytic structures tubulobulbar complexes
Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology, 2018Co-Authors: Arlo Adams, Aarati Sriram, Wayne A VoglAbstract:Sertoli cells of the mammalian seminiferous epithelium form unique subcellular actin-related structures at Intercellular Junctions. The appearance of these so called "tubulobulbar complexes" (TBCs) precedes both sperm release at the apex of the epithelium and the movement of early spermatogenic cells out of the spermatogonial stem cell niche at the base of the epithelium. TBCs are considered to be part of the mechanism of junction endocytosis by Sertoli cells. The structures contain junction proteins and morphologically identifiable Junctions, and are associated with markers of endocytosis. Here we review the current state of knowledge about the structure and function of TBCs. As the complexes form, they morphologically resemble and have the molecular signature of clathrin-coated pits with extremely long necks. As they mature, the actin filament networks around the "necks" of the structures progressively disassemble and the membrane cores expand or swell into distinct "bulbs". These bulbs acquire extensive membrane contact sites with associated cisternae of endoplasmic reticulum. Eventually the bulbs undergo scission and continue through endosomal compartments of the Sertoli cells. The morphology and composition of TBC indicates to us that the structures likely evolved from the basic clathrin-mediated endocytosis mechanism common to cells generally, and along the way they incorporated unique features to accommodate the cyclic turnover of massive and "intact" Intercellular Junctions that occurs during spermatogenesis. Anat Rec, 301:2080-2085, 2018. © 2018 Wiley Periodicals, Inc.
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tubulobulbar complexes are Intercellular podosome like structures that internalize intact Intercellular Junctions during epithelial remodeling events in the rat testis
Biology of Reproduction, 2009Co-Authors: Jnelle S Young, Julian A Guttman, Kuljeet Vaid, Wayne A VoglAbstract:Tubulobulbar complexes are actin-related double-membrane projections that resemble podosomes in other systems and form at Intercellular Junctions in the seminiferous epithelium of the mammalian testis. They are proposed to internalize intact Junctions during sperm release and during the translocation of spermatocytes through basal junction complexes between neighboring Sertoli cells. In this study we probe apical tubulobulbar complexes in fixed epithelial fragments and fixed frozen sections of rat and mouse testes for junction molecules reported to be present at apical sites of attachment (ectoplasmic specializations) between Sertoli cells and spermatids. The adhesion molecules nectin 2 (PVRL2), nectin 3 (PVRL3) and alpha 6 integrin (ITGA6) are present in the elongate parts of tubulobulbar complexes and concentrated at their distal ends. Tubulobulbar complexes contain cortactin (CTTN), a key component of podosomes, and vesicles at the distal ends of tubulobulbar complexes that contain junction molecules are related to early endosome antigen (EEA1). N-cadherin (CDH2), a protein reported to be present at ectoplasmic specializations, is not localized to these unique Junctions or to tubulobulbar complexes but, rather, is primarily concentrated at desmosomes in basal regions of the epithelium. Our results are consistent with the conclusion that tubulobulbar complexes are podosome-like structures that are responsible for internalizing intact Intercellular Junctions during spermatogenesis.
Jing Wang - One of the best experts on this subject based on the ideXlab platform.
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Developmental changes in Intercellular Junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods
Journal of Animal Science and Biotechnology, 2016Co-Authors: Jing Wang, Bie Tan, Guangran Li, Liming Zeng, Xia Xiong, Fengna Li, Xiangfeng Kong, Bo Huang, Gang Liu, Yulong YinAbstract:BackgroundThe intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different Intercellular Junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, Intercellular Junctions and voltage-gated potassium (Kv) channels in the intestine of piglets during the suckling and post-weaning periods.ResultsSamples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen (PCNA)-positive cells and alkaline phosphatase (AKP) activity, as well as the abundances of E-cadherin, occludin, and Kv1.5 mRNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1 to d 21 during the suckling period (P
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Developmental changes in Intercellular Junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods.
Journal of animal science and biotechnology, 2016Co-Authors: Jing Wang, Bie Tan, Liming Zeng, Xia Xiong, Xiangfeng Kong, Bo Huang, Gang Liu, Yulong YinAbstract:Background The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different Intercellular Junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, Intercellular Junctions and voltage-gated potassium (Kv) channels in the intestine of piglets during the suckling and post-weaning periods.
Jnelle S Young - One of the best experts on this subject based on the ideXlab platform.
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tubulobulbar complexes are Intercellular podosome like structures that internalize intact Intercellular Junctions during epithelial remodeling events in the rat testis
Biology of Reproduction, 2009Co-Authors: Jnelle S Young, Julian A Guttman, Kuljeet Vaid, Wayne A VoglAbstract:Tubulobulbar complexes are actin-related double-membrane projections that resemble podosomes in other systems and form at Intercellular Junctions in the seminiferous epithelium of the mammalian testis. They are proposed to internalize intact Junctions during sperm release and during the translocation of spermatocytes through basal junction complexes between neighboring Sertoli cells. In this study we probe apical tubulobulbar complexes in fixed epithelial fragments and fixed frozen sections of rat and mouse testes for junction molecules reported to be present at apical sites of attachment (ectoplasmic specializations) between Sertoli cells and spermatids. The adhesion molecules nectin 2 (PVRL2), nectin 3 (PVRL3) and alpha 6 integrin (ITGA6) are present in the elongate parts of tubulobulbar complexes and concentrated at their distal ends. Tubulobulbar complexes contain cortactin (CTTN), a key component of podosomes, and vesicles at the distal ends of tubulobulbar complexes that contain junction molecules are related to early endosome antigen (EEA1). N-cadherin (CDH2), a protein reported to be present at ectoplasmic specializations, is not localized to these unique Junctions or to tubulobulbar complexes but, rather, is primarily concentrated at desmosomes in basal regions of the epithelium. Our results are consistent with the conclusion that tubulobulbar complexes are podosome-like structures that are responsible for internalizing intact Intercellular Junctions during spermatogenesis.
Detlev Drenckhahn - One of the best experts on this subject based on the ideXlab platform.
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Role of transglutaminase 1 in stabilisation of Intercellular Junctions of the vascular endothelium
Histochemistry and cell biology, 2004Co-Authors: Werner Baumgartner, Nikola Golenhofen, Agnes Weth, Takashi Hiiragi, Rob Saint, Martin Griffin, Detlev DrenckhahnAbstract:Microvascular endothelial monolayers from mouse myocardium (MyEnd) cultured for up to 5 days postconfluency became increasingly resistant to various barrier-compromising stimuli such as low extracellular Ca2+ and treatment with the Ca2+ ionophore A23187 and with the actin depolymerising compound cytochalasin D. In contrast, microvascular endothelial monolayers from mouse lung microvessels (PulmEnd) remained sensitive to these conditions during the entire culture period which corresponds to the well-known in vivo sensitivity of the lung microvasculature to Ca2+depletion and cytochalasin D treatment. One molecular difference between pulmonary and myocardial endothelial cells was found to be transglutaminase 1 (TGase1) which is strongly expressed in myocardial endothelial cells but is absent from pulmonary endothelial cells. Resistance of MyEnd cells to barrier-breaking conditions correlated strongly with translocation of TGase1 to Intercellular Junctions. Simultaneous inhibition of intracellular and extracellular TGase activity by monodansylcadaverine (MDC) strongly weakened barrier properties of MyEnd monolayers, whereas inhibition of extracellular TGases by the membrane-impermeable active site-directed TGase inhibitor R281 did not reduce barrier properties. Weakening of barrier properties could be also induced in MyEnd cells by downregulation of TGase1 expression using RNAi-based gene silencing. These findings suggest that crosslinking activity of intracellular TGase1 at Intercellular Junctions may play a role in controlling barrier properties of endothelial monolayers.