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Meysman F.j.r. - One of the best experts on this subject based on the ideXlab platform.

  • Quantification of Cable Bacteria in Marine Sediments via qPCR
    'Frontiers Media SA', 2020
    Co-Authors: Geelhoed, Jeanine S., Van De Velde, S.j.f., Meysman F.j.r.
    Abstract:

    Cable bacteria (Deltaproteobacteria, Desulfobulbaceae) are long filamentous sulfur-oxidizing bacteria that generate long-distance electric currents running through the bacterial filaments. This way, they couple the oxidation of sulfide in deeper sediment layers to the reduction of oxygen or nitrate near the sediment-water Interface. Cable bacteria are found in a wide range of aquatic sediments, but an accurate procedure to assess their abundance is lacking. We developed a qPCR approach that quantifies Cable bacteria in relation to other bacteria within the family Desulfobulbaceae. Primer sets targeting Cable bacteria, Desulfobulbaceae and the total bacterial community were applied in qPCR with DNA extracted from marine sediment incubations. Amplicon sequencing of the 16S rRNA gene V4 region confirmed that Cable bacteria were accurately enumerated by qPCR, and suggested novel diversity of Cable bacteria. The conjoint quantification of current densities and cell densities revealed that individual filaments carry a mean current of ∼110 pA and have a cell specific oxygen consumption rate of 69 fmol O2 cell–1 day–1. Overall, the qPCR method enables a better quantitative assessment of Cable bacteria abundance, providing new metabolic insights at filament and cell level, and improving our understanding of the microbial ecology of electrogenic sediments.BT/Environmental Biotechnolog

  • Quantification of Cable Bacteria in Marine Sediments via qPCR
    'Frontiers Media SA', 2020
    Co-Authors: Geelhoed, Jeanine S., Van De Velde, S.j.f., Meysman F.j.r.
    Abstract:

    Cable bacteria (Deltaproteobacteria, Desulfobulbaceae) are long filamentous sulfur-oxidizing bacteria that generate long-distance electric currents running through the bacterial filaments. This way, they couple the oxidation of sulfide in deeper sediment layers to the reduction of oxygen or nitrate near the sediment-water Interface. Cable bacteria are found in a wide range of aquatic sediments, but an accurate procedure to assess their abundance is lacking. We developed a qPCR approach that quantifies Cable bacteria in relation to other bacteria within the family Desulfobulbaceae. Primer sets targeting Cable bacteria, Desulfobulbaceae and the total bacterial community were applied in qPCR with DNA extracted from marine sediment incubations. Amplicon sequencing of the 16S rRNA gene V4 region confirmed that Cable bacteria were accurately enumerated by qPCR, and suggested novel diversity of Cable bacteria. The conjoint quantification of current densities and cell densities revealed that individual filaments carry a mean current of ∼110 pA and have a cell specific oxygen consumption rate of 69 fmol O2 cell–1 day–1. Overall, the qPCR method enables a better quantitative assessment of Cable bacteria abundance, providing new metabolic insights at filament and cell level, and improving our understanding of the microbial ecology of electrogenic sediments.

Geelhoed, Jeanine S. - One of the best experts on this subject based on the ideXlab platform.

  • Quantification of Cable Bacteria in Marine Sediments via qPCR
    'Frontiers Media SA', 2020
    Co-Authors: Geelhoed, Jeanine S., Van De Velde, S.j.f., Meysman F.j.r.
    Abstract:

    Cable bacteria (Deltaproteobacteria, Desulfobulbaceae) are long filamentous sulfur-oxidizing bacteria that generate long-distance electric currents running through the bacterial filaments. This way, they couple the oxidation of sulfide in deeper sediment layers to the reduction of oxygen or nitrate near the sediment-water Interface. Cable bacteria are found in a wide range of aquatic sediments, but an accurate procedure to assess their abundance is lacking. We developed a qPCR approach that quantifies Cable bacteria in relation to other bacteria within the family Desulfobulbaceae. Primer sets targeting Cable bacteria, Desulfobulbaceae and the total bacterial community were applied in qPCR with DNA extracted from marine sediment incubations. Amplicon sequencing of the 16S rRNA gene V4 region confirmed that Cable bacteria were accurately enumerated by qPCR, and suggested novel diversity of Cable bacteria. The conjoint quantification of current densities and cell densities revealed that individual filaments carry a mean current of ∼110 pA and have a cell specific oxygen consumption rate of 69 fmol O2 cell–1 day–1. Overall, the qPCR method enables a better quantitative assessment of Cable bacteria abundance, providing new metabolic insights at filament and cell level, and improving our understanding of the microbial ecology of electrogenic sediments.BT/Environmental Biotechnolog

  • Quantification of Cable Bacteria in Marine Sediments via qPCR
    'Frontiers Media SA', 2020
    Co-Authors: Geelhoed, Jeanine S., Van De Velde, S.j.f., Meysman F.j.r.
    Abstract:

    Cable bacteria (Deltaproteobacteria, Desulfobulbaceae) are long filamentous sulfur-oxidizing bacteria that generate long-distance electric currents running through the bacterial filaments. This way, they couple the oxidation of sulfide in deeper sediment layers to the reduction of oxygen or nitrate near the sediment-water Interface. Cable bacteria are found in a wide range of aquatic sediments, but an accurate procedure to assess their abundance is lacking. We developed a qPCR approach that quantifies Cable bacteria in relation to other bacteria within the family Desulfobulbaceae. Primer sets targeting Cable bacteria, Desulfobulbaceae and the total bacterial community were applied in qPCR with DNA extracted from marine sediment incubations. Amplicon sequencing of the 16S rRNA gene V4 region confirmed that Cable bacteria were accurately enumerated by qPCR, and suggested novel diversity of Cable bacteria. The conjoint quantification of current densities and cell densities revealed that individual filaments carry a mean current of ∼110 pA and have a cell specific oxygen consumption rate of 69 fmol O2 cell–1 day–1. Overall, the qPCR method enables a better quantitative assessment of Cable bacteria abundance, providing new metabolic insights at filament and cell level, and improving our understanding of the microbial ecology of electrogenic sediments.

Van De Velde, S.j.f. - One of the best experts on this subject based on the ideXlab platform.

  • Quantification of Cable Bacteria in Marine Sediments via qPCR
    'Frontiers Media SA', 2020
    Co-Authors: Geelhoed, Jeanine S., Van De Velde, S.j.f., Meysman F.j.r.
    Abstract:

    Cable bacteria (Deltaproteobacteria, Desulfobulbaceae) are long filamentous sulfur-oxidizing bacteria that generate long-distance electric currents running through the bacterial filaments. This way, they couple the oxidation of sulfide in deeper sediment layers to the reduction of oxygen or nitrate near the sediment-water Interface. Cable bacteria are found in a wide range of aquatic sediments, but an accurate procedure to assess their abundance is lacking. We developed a qPCR approach that quantifies Cable bacteria in relation to other bacteria within the family Desulfobulbaceae. Primer sets targeting Cable bacteria, Desulfobulbaceae and the total bacterial community were applied in qPCR with DNA extracted from marine sediment incubations. Amplicon sequencing of the 16S rRNA gene V4 region confirmed that Cable bacteria were accurately enumerated by qPCR, and suggested novel diversity of Cable bacteria. The conjoint quantification of current densities and cell densities revealed that individual filaments carry a mean current of ∼110 pA and have a cell specific oxygen consumption rate of 69 fmol O2 cell–1 day–1. Overall, the qPCR method enables a better quantitative assessment of Cable bacteria abundance, providing new metabolic insights at filament and cell level, and improving our understanding of the microbial ecology of electrogenic sediments.BT/Environmental Biotechnolog

  • Quantification of Cable Bacteria in Marine Sediments via qPCR
    'Frontiers Media SA', 2020
    Co-Authors: Geelhoed, Jeanine S., Van De Velde, S.j.f., Meysman F.j.r.
    Abstract:

    Cable bacteria (Deltaproteobacteria, Desulfobulbaceae) are long filamentous sulfur-oxidizing bacteria that generate long-distance electric currents running through the bacterial filaments. This way, they couple the oxidation of sulfide in deeper sediment layers to the reduction of oxygen or nitrate near the sediment-water Interface. Cable bacteria are found in a wide range of aquatic sediments, but an accurate procedure to assess their abundance is lacking. We developed a qPCR approach that quantifies Cable bacteria in relation to other bacteria within the family Desulfobulbaceae. Primer sets targeting Cable bacteria, Desulfobulbaceae and the total bacterial community were applied in qPCR with DNA extracted from marine sediment incubations. Amplicon sequencing of the 16S rRNA gene V4 region confirmed that Cable bacteria were accurately enumerated by qPCR, and suggested novel diversity of Cable bacteria. The conjoint quantification of current densities and cell densities revealed that individual filaments carry a mean current of ∼110 pA and have a cell specific oxygen consumption rate of 69 fmol O2 cell–1 day–1. Overall, the qPCR method enables a better quantitative assessment of Cable bacteria abundance, providing new metabolic insights at filament and cell level, and improving our understanding of the microbial ecology of electrogenic sediments.

Blake, George R. - One of the best experts on this subject based on the ideXlab platform.

  • High Speed Digital Fiber-Optic Links for Control, Video and RF Telemetry Data from Remote Locations
    International Foundation for Telemetering, 1993
    Co-Authors: Blake, George R.
    Abstract:

    International Telemetering Conference Proceedings / October 25-28, 1993 / Riviera Hotel and Convention Center, Las Vegas, NevadaWith the advent of high-speed parallel-to-serial conversion Interface standards reaching the 1.6 gigabit per second region, it is now possible to remote entire telemetry tracking systems over long distances without the need to maintain receivers, combiners, computers and antenna control units at the pedestal. In addition, it will soon be possible to digitize the RF signal(s) with high-speed flash-video analog-to-digital converters and transfer the data over these same links. This coupled with the improvements in fiber-optic L.E.D. and laser transmitters/ receivers and the constantly decreasing cost of fiber-optic Cable, will allow for the eventual elimination of copper cabling for these Interfaces. This will net a cost savings for the end user, provide for easier installations and increase the reliability of the overall system. This paper gives a brief history of the development of high-speed fiber-optic Interface links, then expands into current Interface standards and their utilization (such as MIL-STD-1553B, fiber data distributed Interface (FDDI), HIPPI, etc.). Finally, a glimpse into the future of telemetry systems and the possibilities to soon be realized as major manufacturers of fiber-optic Interfaces push well into the gigabit region. Topics include: • Replacement of existing copper Cables between controller and pedestal using fiber-optic Cable with Interfaces that are not software-based intensive (black boxes); • Multiplexing pedestal control and status lines, RF feed commands, scan reference signals, and boresite video camera control; • Connecting and controlling multiple controllers and/or pedestals through one common Interface Cable; • Operating multiple tracking stations through one remote antenna controller; • Digitized RF telemetry signals sent along with pedestal, feed, video commands and status.International Foundation for TelemeteringProceedings from the International Telemetering Conference are made available by the International Foundation for Telemetering and the University of Arizona Libraries. Visit http://www.telemetry.org/index.php/contact-us if you have questions about items in this collection

H De Paepe - One of the best experts on this subject based on the ideXlab platform.

  • percutaneous electrical nerve stimulation in children with therapy resistant nonneuropathic bladder sphincter dysfunction a pilot study
    The Journal of Urology, 2002
    Co-Authors: P. Hoebeke, Vande J Walle, C Renson, L Petillon, H De Paepe
    Abstract:

    ABSTRACTPurpose: We evaluated the effect of percutaneous electrical nerve stimulation on voiding dysfunction in a group of children with therapy resistant, nonneuropathic bladder sphincter dysfunction.Materials and Methods: In a prospective study 17 boys and 15 girls with mean age of 11.7 years underwent percutaneous electrical nerve stimulation after the failure of more than 2 years of urological and/or pharmacotherapy. The device for percutaneous electrical nerve stimulation consists of an Interface Cable, a surface electrode, a percutaneous needle and a portable stimulator. The needle is inserted at the tibial nerve level and a portable stimulator provided pulsations at a frequency of 20 Hz. for 30 minutes once weekly. Every 6 weeks the children were evaluated. Evaluation parameters were urgency, daytime incontinence, voiding frequency, the uroflowmetry curve and bladder capacity. When favorable results were observed after 6 sessions, therapy was continued for another 12 sessions. In 24 children antich...