The Experts below are selected from a list of 3441 Experts worldwide ranked by ideXlab platform
J. Konečný - One of the best experts on this subject based on the ideXlab platform.
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Perforation Peritonitis in Primary Intestinal Tuberculosis
Digestive surgery, 2001Co-Authors: R. Šefr, P. Rotterová, J. KonečnýAbstract:Primary Intestinal Tuberculosis is unusual in European and North American countries today. Its diagnosis is often surprising and differentiation from inflammatory bowel diseases is difficult. The auth
Sun-joo Kim - One of the best experts on this subject based on the ideXlab platform.
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7113 The value of nested polymerase chain reaction in diagnosis of Intestinal Tuberculosis.
Gastrointestinal Endoscopy, 2000Co-Authors: Moon-ho Lee, Eun Joo Kim, Il-kwon Chung, Hong-soo Kim, Sang-heum Park, Sun-joo KimAbstract:Background) The pathologic examination, the demonstration of AFB bacilli and cultural confirmation of mycobacterium Tuberculosis from endoscopically biopsied mucosal specimens are required to establish non-operative diagnosis of Intestinal Tuberculosis. But it is often difficult to achieve because of a low sensitivity and/or a low specificity of each methods. Recently it was known that PCR assay provides a rapid ,sensitive, reliable methods to detect mycobacterum Tuberculosis in tissue specimens. We investigated the usefulness of nested PCR to identify mycobacterium Tuberculosis DNA in endoscopically biopsied specimens (formalin fixed, paraffin-embbeded tissue) of Intestinal Tuberculosis. Specimens from Crohn's disease and colon cancer were used as a negative control of nested PCR assay. Patients and Methods) 22 endoscopically biopsied specimens of Intestinal Tuberculosis confirmed by pathology, microbiology and clinical course were investigated. 8 specimens of Crohn's disease and 8 specimens of colon cancer were also analyzed. DNA was extracted from paraffinembedded tissue. The target DNA was a repetitive DNA(IS6110) of mycobacterium Tuberculosis. The primers for the first PCR were KBN5 and KBN6 (324 bp) and the primer for the second PCR were KBN7 and KBN8 (285bp). Results) M.Tuberculosis DNA was not detected in all specimens of Crohn's disease and colon cancer. 36.4% (8/22) of Intestinal Tuberculosis revealed a positive result for M.Tuberculosis DNA. The diagnostic rate of Intestinal Tuberculosis by pathologic examination was 32%. Pathologic examination combined with microbiologic examination raised the diagnostic rate to 55%. The diagnostic rate of Intestinal Tuberculosis by PCR combined with pathologic and microbiologic examination was 68%. Conclusion) The sensitivity of nested PCR for diagnosing Intestinal Tuberculosis in endoscopically biopsied specimens was not higher than expected, but PCR assay combined with conventional methods may give a better result for the endoscopic diagnosis of Intestinal Tuberculosis.
R. Šefr - One of the best experts on this subject based on the ideXlab platform.
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Perforation Peritonitis in Primary Intestinal Tuberculosis
Digestive surgery, 2001Co-Authors: R. Šefr, P. Rotterová, J. KonečnýAbstract:Primary Intestinal Tuberculosis is unusual in European and North American countries today. Its diagnosis is often surprising and differentiation from inflammatory bowel diseases is difficult. The auth
Moon-ho Lee - One of the best experts on this subject based on the ideXlab platform.
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7113 The value of nested polymerase chain reaction in diagnosis of Intestinal Tuberculosis.
Gastrointestinal Endoscopy, 2000Co-Authors: Moon-ho Lee, Eun Joo Kim, Il-kwon Chung, Hong-soo Kim, Sang-heum Park, Sun-joo KimAbstract:Background) The pathologic examination, the demonstration of AFB bacilli and cultural confirmation of mycobacterium Tuberculosis from endoscopically biopsied mucosal specimens are required to establish non-operative diagnosis of Intestinal Tuberculosis. But it is often difficult to achieve because of a low sensitivity and/or a low specificity of each methods. Recently it was known that PCR assay provides a rapid ,sensitive, reliable methods to detect mycobacterum Tuberculosis in tissue specimens. We investigated the usefulness of nested PCR to identify mycobacterium Tuberculosis DNA in endoscopically biopsied specimens (formalin fixed, paraffin-embbeded tissue) of Intestinal Tuberculosis. Specimens from Crohn's disease and colon cancer were used as a negative control of nested PCR assay. Patients and Methods) 22 endoscopically biopsied specimens of Intestinal Tuberculosis confirmed by pathology, microbiology and clinical course were investigated. 8 specimens of Crohn's disease and 8 specimens of colon cancer were also analyzed. DNA was extracted from paraffinembedded tissue. The target DNA was a repetitive DNA(IS6110) of mycobacterium Tuberculosis. The primers for the first PCR were KBN5 and KBN6 (324 bp) and the primer for the second PCR were KBN7 and KBN8 (285bp). Results) M.Tuberculosis DNA was not detected in all specimens of Crohn's disease and colon cancer. 36.4% (8/22) of Intestinal Tuberculosis revealed a positive result for M.Tuberculosis DNA. The diagnostic rate of Intestinal Tuberculosis by pathologic examination was 32%. Pathologic examination combined with microbiologic examination raised the diagnostic rate to 55%. The diagnostic rate of Intestinal Tuberculosis by PCR combined with pathologic and microbiologic examination was 68%. Conclusion) The sensitivity of nested PCR for diagnosing Intestinal Tuberculosis in endoscopically biopsied specimens was not higher than expected, but PCR assay combined with conventional methods may give a better result for the endoscopic diagnosis of Intestinal Tuberculosis.
Fergus Shanahan - One of the best experts on this subject based on the ideXlab platform.
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Intestinal Tuberculosis mimicking Crohn's disease: lessons relearned in a new era.
European journal of gastroenterology & hepatology, 2007Co-Authors: Vikrant Sibartie, William O. Kirwan, Seamus O'mahony, W. A. Stack, Fergus ShanahanAbstract:Over 400 000 cases of Tuberculosis existed in Europe in 2002, 1% of which were Intestinal Tuberculosis. With population migrations on the increase, physicians may have to face an increase in Intestinal Tuberculosis. One of the attributes of Intestinal Tuberculosis is its ability to present in nonspecific ways and to mimic other disorders, in particular inflammatory bowel disease. We present a case series of Intestinal Tuberculosis presenting as inflammatory bowel disease and referred for management to a specialized clinic in inflammatory bowel disease, followed by a discussion of the difficulties encountered with this condition. We highlight the consequences that misdiagnosis can have, in an era where population demographics are changing in Europe and where immunomodulators and biological agents have the potential to do more harm than good.
