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John P. Geibel - One of the best experts on this subject based on the ideXlab platform.
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a new method to measure intestinal secretion using fluorescein isothiocyanate inulin in small bowel of rats
Journal of Surgical Research, 2015Co-Authors: Armando Salim Munozabraham, Sami S Judeeba, Abedalrazaq Alkukhun, Tariq I Alfadda, Roger Patronlozano, Manuel I Rodriguezdavalos, John P. GeibelAbstract:Abstract Background Small Intestine Ischemia can be seen in various conditions such as intestinal transplantation. To further understand the pathologic disruption in Ischemia–reperfusion injury, we have developed a method to measure fluid changes in the intestinal lumen of rats. Methods Two 10-cm rat Intestine segments were procured, connected to the terminal apertures of a perfusion device, and continuously infused with 3 mL of HEPES solution (control solution) containing 50 μM of fluorescein isothiocyanate (FITC)-inulin. The perfusion device consists of concentric chambers that contain the perfused bowel segments, which are maintained at 37°C via H2O bath. The individual chamber has four apertures as follows: two fill and/or drain the surrounding HEPES solution on the blood side of the tissue. The others provide flow of HEPES solution containing FITC-inulin through the lumens. The experimental Intestine was infused with the same solution with 100 μM of Forskolin. A pump continuously circulated solutions at 6 mL/min. Samples were collected at 15-min intervals until 150 min and were measured by the nanoflourospectrometer. Results A mean of 6-μM decrease in the FITC-inulin concentration in the Forskolin-treated experimental Intestine was observed in comparison with that in the control Intestine. The FITC-inulin count dilution in the experimental Intestine is a result of an increase of fluid secretion produced by the effect of Forskolin, with P values Conclusions We demonstrate that it is possible to measure luminal fluid changes over time using our new modified perfusion system along with FITC-inulin to allow real-time determinations of fluid and/or electrolyte movement along the small Intestine.
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a new method to measure intestinal secretion using fluorescein isothiocyanate inulin in small bowel of rats
Journal of Surgical Research, 2015Co-Authors: Armando Salim Munozabraham, Sami S Judeeba, Abedalrazaq Alkukhun, Tariq I Alfadda, Roger Patronlozano, Manuel I Rodriguezdavalos, John P. GeibelAbstract:Abstract Background Small Intestine Ischemia can be seen in various conditions such as intestinal transplantation. To further understand the pathologic disruption in Ischemia–reperfusion injury, we have developed a method to measure fluid changes in the intestinal lumen of rats. Methods Two 10-cm rat Intestine segments were procured, connected to the terminal apertures of a perfusion device, and continuously infused with 3 mL of HEPES solution (control solution) containing 50 μM of fluorescein isothiocyanate (FITC)-inulin. The perfusion device consists of concentric chambers that contain the perfused bowel segments, which are maintained at 37°C via H2O bath. The individual chamber has four apertures as follows: two fill and/or drain the surrounding HEPES solution on the blood side of the tissue. The others provide flow of HEPES solution containing FITC-inulin through the lumens. The experimental Intestine was infused with the same solution with 100 μM of Forskolin. A pump continuously circulated solutions at 6 mL/min. Samples were collected at 15-min intervals until 150 min and were measured by the nanoflourospectrometer. Results A mean of 6-μM decrease in the FITC-inulin concentration in the Forskolin-treated experimental Intestine was observed in comparison with that in the control Intestine. The FITC-inulin count dilution in the experimental Intestine is a result of an increase of fluid secretion produced by the effect of Forskolin, with P values Conclusions We demonstrate that it is possible to measure luminal fluid changes over time using our new modified perfusion system along with FITC-inulin to allow real-time determinations of fluid and/or electrolyte movement along the small Intestine.
V.i. Novoselov - One of the best experts on this subject based on the ideXlab platform.
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the effect of exogenous peroxiredoxin 6 on the state of mesenteric vessels and the small Intestine in Ischemia reperfusion injury
Biophysics, 2017Co-Authors: M G Sharapov, A E Gordeeva, V. K. Ravin, R G Goncharov, Irina V. Tikhonova, A A Temnov, Evgenevich Evgenij Fesenko, V.i. NovoselovAbstract:Oxidative stress is the main component of pathogenesis in Ischemia–reperfusion injury. The administration of exogenous antioxidants suppresses oxidative stress and may decrease the severity of Ischemia–reperfusion injury. The Intestine is one of the most sensitive organs to the effect of Ischemia–reperfusion. A rat model of a small Intestine Ischemia–reperfusion injury, based on occlusion of the superior mesenteric artery, was used in this work. Recombinant peroxiredoxin 6, a representative of an ancient family of peroxidases that are able to neutralize a broad range of both organic and inorganic peroxides, was used as an exogenous antioxidant. The intravenous administration of the exogenous peroxiredoxin 6 prior to Ischemia–reperfusion minimizes tissue injury and reduces apoptotic cell death in the Intestine and the mesenteric vessels. The impact of the exogenous peroxiredoxin 6 upon the NO level elevation in animal blood has been shown to be correlated with the enhanced inducible NO synthase expression. Thus, the use of exogenous peroxiredoxin 6 in Ischemia–reperfusion injury of the Intestine and the mesenteric vessels promotes normalization of the tissue redox homeostasis, structure protection, and restoration of the microvasculature.
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protective effect of peroxiredoxin 6 in Ischemia reperfusion induced damage of small Intestine
Digestive Diseases and Sciences, 2015Co-Authors: A E Gordeeva, M G Sharapov, A A Temnov, A. A. Charnagalov, Evgenevich Evgenij Fesenko, V.i. NovoselovAbstract:Strong oxidative stress starting in the epithelium upon restoration of blood cell circulation is a major cause of necrosis of the intestinal epithelium in Ischemia/reperfusion-induced damage. The purpose of this study was to investigate the tissue-protective effect of exogenous peroxiredoxin 6 (Prx6) in Ischemia/reperfusion-induced damage of small Intestine. The research was carried out using a model of acute superior mesenteric artery occlusion in Wistar male rats. Exogenous Prx6 was administrated intravenously 15 min prior to small Intestine Ischemia. The distribution of endogenous Prx6 in the small Intestine was determined by immunohistochemical analysis. The expression level of antioxidant enzymes was evaluated by RT-PCR in real time. Exogenous Prx6 injected to animals intravenously was detected in blood vessel lumens, and its diffuse distribution was subsequently confirmed in the intestinal epithelium. Expression analysis of genes coding for major antioxidant enzymes demonstrated a significant activation of SOD 1, SOD 3, Prx6, GPx2, GPx7 expression during I/R-induced damage of the small Intestine. Injection of exogenous Prx6 prior to induced Ischemia resulted in minimization of oxidative injury by reducing necrosis and apoptosis, by normalization of gene activity of antioxidant enzyme. It eventually led to a reduction of epithelium destruction in the small Intestine. By contrast, administration of a purified mutant form of Prx6 (Prx6C47S) without peroxidase activity had no protective effect. The application of exogenous Prx6 enables normalization of the antioxidant status of the small Intestine and reduction of cell destruction upon I/R-induced organ damage.
Weiwei Ding - One of the best experts on this subject based on the ideXlab platform.
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Comparisons of three surgical procedures on Intestine Ischemia reperfusion injury in a superior mesenteric artery injury model.
The Journal of surgical research, 2009Co-Authors: Weiwei Ding, Kun ZhaoAbstract:Background Temporary ligation, primary anastomosis, and temporary shunt have been reported to deal with superior mesenteric artery (SMA) injuries. We aimed to investigate which brought minimal Ischemia reperfusion injury in a hypothermic traumatic shock swine model. Methods SMA was completely clamped while pigs were hemorrhaged to a mean arterial pressure (MAP) of 40mm Hg. Animals were then randomized into temporary ligation (A, n = 8), primary anastomosis (B, n = 8), temporary shunt (C, n = 8), and control groups (n = 4). Animals in group A remained SMA interrupted for additional 1h while the other groups underwent the corresponding procedures immediately. Intestine injury was assessed by histologic examination and measurement of lipid peroxidations at the end of Ischemia and experiment. Results Overall mortality rate was 50%, 25%, and 0% in groups A, B, and C, respectively (P Conclusion Our study suggests that temporary shunt insertion might be preferred as it shortens Ischemia time, alleviates intestinal Ischemia/reperfusion injury, and thus decreases early mortality in this animal model.
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establishment of an acute superior mesenteric artery injury model for damage control surgery
Journal of Surgical Research, 2009Co-Authors: Weiwei Ding, Xingjiang Wu, Guanwen Gong, Qingxin Meng, Lideng Ni, Jieshou LiAbstract:Background Managements of superior mesenteric artery (SMA) injuries are difficult and often result in a disappointing outcome. Damage control surgery (DCS) has been approved to be an effective and reliable strategy for severe trauma victims. We aimed to build up a severe trauma-shock-hypothermia model of SMA injuries for DCS study and determine the optimal time to institute DCS. Methods Pigs were anesthetized and instrumented with arterial and a thermodilution cardiac output catheter. SMA flow was interrupted while animals were hemorrhaged to 45% estimated blood volume. Pigs were maintained shock and Intestine Ischemia for three durations: Intestine Ischemia for 30 min (I-30; n = 6), 60 min (I-60; n = 6), and 90 min (I-90; n = 6). Cold lactated Ringer's (10 mL/kg) was infused to induce hypothermia. SMA was then declamped and kept in reperfusion for 6 h. Hemodynamic data and serum samples were collected during shock and resuscitation. Distal ileum was collected at the end of Ischemia and reperfusion. Results All animals presented with disastrous conditions at the end of Ischemia: low temperature, severe acidosis, decreased blood pressure, depressed cardiac output, and oxygen delivery. I-90 animals suffered the lowest temperature, the most severe acidosis, lowest blood pressure, and depressed cardiac output and oxygen delivery. Coagulopathy developed in I-90, whereas normal prothrombin time and thrombin time were detected in I-30 and I-60. Aspartate aminotransferase, lactate dehydrogenase, creatine kinase, and alkaline phosphatase were equally within groups (P > 0.05). All (6/6) of I-30, 83.3% (5/6) of I-60, and 16.7% (1/6) of I-90 pigs survived (P Conclusions We first built up an acute SMA injury animal model for DCS investigations and determined that the optimal institution time of DCS was before 60 min after SMA injury in the trauma-shock-hypothermia swine model.
Armando Salim Munozabraham - One of the best experts on this subject based on the ideXlab platform.
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a new method to measure intestinal secretion using fluorescein isothiocyanate inulin in small bowel of rats
Journal of Surgical Research, 2015Co-Authors: Armando Salim Munozabraham, Sami S Judeeba, Abedalrazaq Alkukhun, Tariq I Alfadda, Roger Patronlozano, Manuel I Rodriguezdavalos, John P. GeibelAbstract:Abstract Background Small Intestine Ischemia can be seen in various conditions such as intestinal transplantation. To further understand the pathologic disruption in Ischemia–reperfusion injury, we have developed a method to measure fluid changes in the intestinal lumen of rats. Methods Two 10-cm rat Intestine segments were procured, connected to the terminal apertures of a perfusion device, and continuously infused with 3 mL of HEPES solution (control solution) containing 50 μM of fluorescein isothiocyanate (FITC)-inulin. The perfusion device consists of concentric chambers that contain the perfused bowel segments, which are maintained at 37°C via H2O bath. The individual chamber has four apertures as follows: two fill and/or drain the surrounding HEPES solution on the blood side of the tissue. The others provide flow of HEPES solution containing FITC-inulin through the lumens. The experimental Intestine was infused with the same solution with 100 μM of Forskolin. A pump continuously circulated solutions at 6 mL/min. Samples were collected at 15-min intervals until 150 min and were measured by the nanoflourospectrometer. Results A mean of 6-μM decrease in the FITC-inulin concentration in the Forskolin-treated experimental Intestine was observed in comparison with that in the control Intestine. The FITC-inulin count dilution in the experimental Intestine is a result of an increase of fluid secretion produced by the effect of Forskolin, with P values Conclusions We demonstrate that it is possible to measure luminal fluid changes over time using our new modified perfusion system along with FITC-inulin to allow real-time determinations of fluid and/or electrolyte movement along the small Intestine.
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a new method to measure intestinal secretion using fluorescein isothiocyanate inulin in small bowel of rats
Journal of Surgical Research, 2015Co-Authors: Armando Salim Munozabraham, Sami S Judeeba, Abedalrazaq Alkukhun, Tariq I Alfadda, Roger Patronlozano, Manuel I Rodriguezdavalos, John P. GeibelAbstract:Abstract Background Small Intestine Ischemia can be seen in various conditions such as intestinal transplantation. To further understand the pathologic disruption in Ischemia–reperfusion injury, we have developed a method to measure fluid changes in the intestinal lumen of rats. Methods Two 10-cm rat Intestine segments were procured, connected to the terminal apertures of a perfusion device, and continuously infused with 3 mL of HEPES solution (control solution) containing 50 μM of fluorescein isothiocyanate (FITC)-inulin. The perfusion device consists of concentric chambers that contain the perfused bowel segments, which are maintained at 37°C via H2O bath. The individual chamber has four apertures as follows: two fill and/or drain the surrounding HEPES solution on the blood side of the tissue. The others provide flow of HEPES solution containing FITC-inulin through the lumens. The experimental Intestine was infused with the same solution with 100 μM of Forskolin. A pump continuously circulated solutions at 6 mL/min. Samples were collected at 15-min intervals until 150 min and were measured by the nanoflourospectrometer. Results A mean of 6-μM decrease in the FITC-inulin concentration in the Forskolin-treated experimental Intestine was observed in comparison with that in the control Intestine. The FITC-inulin count dilution in the experimental Intestine is a result of an increase of fluid secretion produced by the effect of Forskolin, with P values Conclusions We demonstrate that it is possible to measure luminal fluid changes over time using our new modified perfusion system along with FITC-inulin to allow real-time determinations of fluid and/or electrolyte movement along the small Intestine.
Kun Zhao - One of the best experts on this subject based on the ideXlab platform.
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Comparisons of three surgical procedures on Intestine Ischemia reperfusion injury in a superior mesenteric artery injury model.
The Journal of surgical research, 2009Co-Authors: Weiwei Ding, Kun ZhaoAbstract:Background Temporary ligation, primary anastomosis, and temporary shunt have been reported to deal with superior mesenteric artery (SMA) injuries. We aimed to investigate which brought minimal Ischemia reperfusion injury in a hypothermic traumatic shock swine model. Methods SMA was completely clamped while pigs were hemorrhaged to a mean arterial pressure (MAP) of 40mm Hg. Animals were then randomized into temporary ligation (A, n = 8), primary anastomosis (B, n = 8), temporary shunt (C, n = 8), and control groups (n = 4). Animals in group A remained SMA interrupted for additional 1h while the other groups underwent the corresponding procedures immediately. Intestine injury was assessed by histologic examination and measurement of lipid peroxidations at the end of Ischemia and experiment. Results Overall mortality rate was 50%, 25%, and 0% in groups A, B, and C, respectively (P Conclusion Our study suggests that temporary shunt insertion might be preferred as it shortens Ischemia time, alleviates intestinal Ischemia/reperfusion injury, and thus decreases early mortality in this animal model.
