The Experts below are selected from a list of 78 Experts worldwide ranked by ideXlab platform
Per Stahl Skov - One of the best experts on this subject based on the ideXlab platform.
-
is a positive Intracutaneous Test induced by penicillin mediated by histamine a cutaneous microdialysis study in penicillin allergic patients
Clinical and Translational Allergy, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslevjensen, Per Stahl SkovAbstract:Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive. To investigate real-time histamine release from a positive Intracutaneous Test induced by penicillin in patients with positive and negative challenges to penicillin. Skin microdialysis was performed in 21 penicillin-allergic patients with positive skin Test, 13 non-allergic volunteers serving as negative controls, and 7 grass pollen-allergic patients serving as positive controls. Histamine was measured by microdialysis after skin Test with penicillin/grass/NaCl. Penicillin challenge was subsequently performed in 12 of the patients. Only 10/21 patients (47.6%) were skin Test positive at microdialysis. During microdialysis 13 single Intracutaneous Tests were positive and histamine was detected in 4/13 occurring in four challenge positive patients. Thirteen/21 patients (61.9%) were deemed allergic to penicillin; eight had positive skin Test. Two patients with positive skin Test were challenge negative. In grass pollen allergic patients, 7/7 had a positive Intracutaneous Test to grass and all released histamine in the wheals. All 13 negative controls had negative Intracutaneous Test to penicillin and no histamine release. Histamine was only detected in the minority of positive Intracutaneous Tests with penicillin in penicillin-allergic patients. Other mediators may be involved.
-
is a positive Intracutaneous Test induced by penicillin mediated by histamine a cutaneous microdialysis study in penicillin allergic patients
Clinical and Translational Allergy, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslevjensen, Per Stahl SkovAbstract:Background Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive.
-
Is a positive Intracutaneous Test induced by penicillin mediated by histamine? A cutaneous microdialysis study in penicillin-allergic patients
BMC, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslev-jensen, Per Stahl SkovAbstract:Abstract Background Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive. Objective To investigate real-time histamine release from a positive Intracutaneous Test induced by penicillin in patients with positive and negative challenges to penicillin. Methods Skin microdialysis was performed in 21 penicillin-allergic patients with positive skin Test, 13 non-allergic volunteers serving as negative controls, and 7 grass pollen-allergic patients serving as positive controls. Histamine was measured by microdialysis after skin Test with penicillin/grass/NaCl. Penicillin challenge was subsequently performed in 12 of the patients. Results Only 10/21 patients (47.6%) were skin Test positive at microdialysis. During microdialysis 13 single Intracutaneous Tests were positive and histamine was detected in 4/13 occurring in four challenge positive patients. Thirteen/21 patients (61.9%) were deemed allergic to penicillin; eight had positive skin Test. Two patients with positive skin Test were challenge negative. In grass pollen allergic patients, 7/7 had a positive Intracutaneous Test to grass and all released histamine in the wheals. All 13 negative controls had negative Intracutaneous Test to penicillin and no histamine release. Conclusion Histamine was only detected in the minority of positive Intracutaneous Tests with penicillin in penicillin-allergic patients. Other mediators may be involved
-
Intracutaneous injection of autologous serum with specific IgE against penicillin followed by Intracutaneous Test with penicillin, does not elicit a positive Intracutaneous Test
Clinical and Translational Allergy, 2015Co-Authors: Line K. Tannert, Sidsel Falkencrone, Per Stahl Skov, Charlotte Gotthard Moertz, Carsten Bindslev-jensenAbstract:Background In patients allergic to penicillin only a minority of those who have specific IgE against penicillin also have a positive Intracutaneous Test (ICT) to penicillin and vice versa. This is in contrast to findings in patients with allergy to classical type 1 allergens, e.g. grass or peanuts. Besides that, our investigations indicates that histamine is only released in the minority of positive Intracutaneous Tests. The reason for this is not known. A hypothesis could be that the specific IgE against penicillin measured in serum is not present in the skin. The aim of this project is to investigate, if you by priming the skin with autologous serum in patients with specific IgE to penicillin subsequently are able to develop a positive ICT upon Testing.
Line K. Tannert - One of the best experts on this subject based on the ideXlab platform.
-
is a positive Intracutaneous Test induced by penicillin mediated by histamine a cutaneous microdialysis study in penicillin allergic patients
Clinical and Translational Allergy, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslevjensen, Per Stahl SkovAbstract:Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive. To investigate real-time histamine release from a positive Intracutaneous Test induced by penicillin in patients with positive and negative challenges to penicillin. Skin microdialysis was performed in 21 penicillin-allergic patients with positive skin Test, 13 non-allergic volunteers serving as negative controls, and 7 grass pollen-allergic patients serving as positive controls. Histamine was measured by microdialysis after skin Test with penicillin/grass/NaCl. Penicillin challenge was subsequently performed in 12 of the patients. Only 10/21 patients (47.6%) were skin Test positive at microdialysis. During microdialysis 13 single Intracutaneous Tests were positive and histamine was detected in 4/13 occurring in four challenge positive patients. Thirteen/21 patients (61.9%) were deemed allergic to penicillin; eight had positive skin Test. Two patients with positive skin Test were challenge negative. In grass pollen allergic patients, 7/7 had a positive Intracutaneous Test to grass and all released histamine in the wheals. All 13 negative controls had negative Intracutaneous Test to penicillin and no histamine release. Histamine was only detected in the minority of positive Intracutaneous Tests with penicillin in penicillin-allergic patients. Other mediators may be involved.
-
is a positive Intracutaneous Test induced by penicillin mediated by histamine a cutaneous microdialysis study in penicillin allergic patients
Clinical and Translational Allergy, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslevjensen, Per Stahl SkovAbstract:Background Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive.
-
Is a positive Intracutaneous Test induced by penicillin mediated by histamine? A cutaneous microdialysis study in penicillin-allergic patients
BMC, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslev-jensen, Per Stahl SkovAbstract:Abstract Background Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive. Objective To investigate real-time histamine release from a positive Intracutaneous Test induced by penicillin in patients with positive and negative challenges to penicillin. Methods Skin microdialysis was performed in 21 penicillin-allergic patients with positive skin Test, 13 non-allergic volunteers serving as negative controls, and 7 grass pollen-allergic patients serving as positive controls. Histamine was measured by microdialysis after skin Test with penicillin/grass/NaCl. Penicillin challenge was subsequently performed in 12 of the patients. Results Only 10/21 patients (47.6%) were skin Test positive at microdialysis. During microdialysis 13 single Intracutaneous Tests were positive and histamine was detected in 4/13 occurring in four challenge positive patients. Thirteen/21 patients (61.9%) were deemed allergic to penicillin; eight had positive skin Test. Two patients with positive skin Test were challenge negative. In grass pollen allergic patients, 7/7 had a positive Intracutaneous Test to grass and all released histamine in the wheals. All 13 negative controls had negative Intracutaneous Test to penicillin and no histamine release. Conclusion Histamine was only detected in the minority of positive Intracutaneous Tests with penicillin in penicillin-allergic patients. Other mediators may be involved
-
Intracutaneous injection of autologous serum with specific IgE against penicillin followed by Intracutaneous Test with penicillin, does not elicit a positive Intracutaneous Test
Clinical and Translational Allergy, 2015Co-Authors: Line K. Tannert, Sidsel Falkencrone, Per Stahl Skov, Charlotte Gotthard Moertz, Carsten Bindslev-jensenAbstract:Background In patients allergic to penicillin only a minority of those who have specific IgE against penicillin also have a positive Intracutaneous Test (ICT) to penicillin and vice versa. This is in contrast to findings in patients with allergy to classical type 1 allergens, e.g. grass or peanuts. Besides that, our investigations indicates that histamine is only released in the minority of positive Intracutaneous Tests. The reason for this is not known. A hypothesis could be that the specific IgE against penicillin measured in serum is not present in the skin. The aim of this project is to investigate, if you by priming the skin with autologous serum in patients with specific IgE to penicillin subsequently are able to develop a positive ICT upon Testing.
Sidsel Falkencrone - One of the best experts on this subject based on the ideXlab platform.
-
is a positive Intracutaneous Test induced by penicillin mediated by histamine a cutaneous microdialysis study in penicillin allergic patients
Clinical and Translational Allergy, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslevjensen, Per Stahl SkovAbstract:Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive. To investigate real-time histamine release from a positive Intracutaneous Test induced by penicillin in patients with positive and negative challenges to penicillin. Skin microdialysis was performed in 21 penicillin-allergic patients with positive skin Test, 13 non-allergic volunteers serving as negative controls, and 7 grass pollen-allergic patients serving as positive controls. Histamine was measured by microdialysis after skin Test with penicillin/grass/NaCl. Penicillin challenge was subsequently performed in 12 of the patients. Only 10/21 patients (47.6%) were skin Test positive at microdialysis. During microdialysis 13 single Intracutaneous Tests were positive and histamine was detected in 4/13 occurring in four challenge positive patients. Thirteen/21 patients (61.9%) were deemed allergic to penicillin; eight had positive skin Test. Two patients with positive skin Test were challenge negative. In grass pollen allergic patients, 7/7 had a positive Intracutaneous Test to grass and all released histamine in the wheals. All 13 negative controls had negative Intracutaneous Test to penicillin and no histamine release. Histamine was only detected in the minority of positive Intracutaneous Tests with penicillin in penicillin-allergic patients. Other mediators may be involved.
-
is a positive Intracutaneous Test induced by penicillin mediated by histamine a cutaneous microdialysis study in penicillin allergic patients
Clinical and Translational Allergy, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslevjensen, Per Stahl SkovAbstract:Background Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive.
-
Is a positive Intracutaneous Test induced by penicillin mediated by histamine? A cutaneous microdialysis study in penicillin-allergic patients
BMC, 2017Co-Authors: Line K. Tannert, Sidsel Falkencrone, Charlotte G. Mortz, Carsten Bindslev-jensen, Per Stahl SkovAbstract:Abstract Background Diagnostic workup of penicillin allergy comprises skin Testing with penicillins, and patients are deemed allergic if skin Test is positive. However, the literature suggests that skin Test-positive patients may be challenge-negative, indicating that the skin Test may be falsely positive. Objective To investigate real-time histamine release from a positive Intracutaneous Test induced by penicillin in patients with positive and negative challenges to penicillin. Methods Skin microdialysis was performed in 21 penicillin-allergic patients with positive skin Test, 13 non-allergic volunteers serving as negative controls, and 7 grass pollen-allergic patients serving as positive controls. Histamine was measured by microdialysis after skin Test with penicillin/grass/NaCl. Penicillin challenge was subsequently performed in 12 of the patients. Results Only 10/21 patients (47.6%) were skin Test positive at microdialysis. During microdialysis 13 single Intracutaneous Tests were positive and histamine was detected in 4/13 occurring in four challenge positive patients. Thirteen/21 patients (61.9%) were deemed allergic to penicillin; eight had positive skin Test. Two patients with positive skin Test were challenge negative. In grass pollen allergic patients, 7/7 had a positive Intracutaneous Test to grass and all released histamine in the wheals. All 13 negative controls had negative Intracutaneous Test to penicillin and no histamine release. Conclusion Histamine was only detected in the minority of positive Intracutaneous Tests with penicillin in penicillin-allergic patients. Other mediators may be involved
-
Intracutaneous injection of autologous serum with specific IgE against penicillin followed by Intracutaneous Test with penicillin, does not elicit a positive Intracutaneous Test
Clinical and Translational Allergy, 2015Co-Authors: Line K. Tannert, Sidsel Falkencrone, Per Stahl Skov, Charlotte Gotthard Moertz, Carsten Bindslev-jensenAbstract:Background In patients allergic to penicillin only a minority of those who have specific IgE against penicillin also have a positive Intracutaneous Test (ICT) to penicillin and vice versa. This is in contrast to findings in patients with allergy to classical type 1 allergens, e.g. grass or peanuts. Besides that, our investigations indicates that histamine is only released in the minority of positive Intracutaneous Tests. The reason for this is not known. A hypothesis could be that the specific IgE against penicillin measured in serum is not present in the skin. The aim of this project is to investigate, if you by priming the skin with autologous serum in patients with specific IgE to penicillin subsequently are able to develop a positive ICT upon Testing.
John B. Bass - One of the best experts on this subject based on the ideXlab platform.
-
the tuberculin skin Test
Clinical Infectious Diseases, 1993Co-Authors: Robin E Huebner, Maybelle F Schein, John B. BassAbstract:nounced the discovery of a cure for tuberculosis. The cure consisted of giving patients subcutaneous doses of tuberculin, a brownish, transparent liquid obtained from culture filtrates of MWcobacterium tuberculosis. This treatment caused a febrile reaction within 4 or 5 hours. In most cases, the fever was accompanied by vomiting, rigors, and other constitutional symptoms. Koch reported that the administration of increasing daily doses of tuberculin resulted in the rapid healing of mild cases of tuberculosis and in slow, progressive improvement in more serious cases [1]. Patients who did not have tuberculosis experienced slight pains in the limbs and transient fatigue when injected with tuberculin. Less than a year after the announcement, Koch's tuberculin was disproved as a cure for consumption [1]. Koch did not realize that he had discovered what would become one of the most widely used diagnostic Tests ever developed. The observation of different responses to tuberculin in patients with and without tuberculosis led to the development of new methods of administering the antigen. The use of these new methods eliminated systemic symptoms but caused a local reaction at the injection site. Various methods of administration were Tested, including the Pirquet cutaneous Test, the Moro percutaneous patch Test, the Mantoux Intracutaneous Test, and the Calmette conjunctival Test [1]. The first three methods remain in use. By the early 1930s tuberculin skin Testing had become a method for screening apparently healthy persons for infection with M. tuberculosis. At that time it was common practice to give a series of four or five graded doses of tuberculin before excluding the possibility of tuberculous infection. As the use of the tuberculin skin Test increased, evidence accumulated that not all reactions to purified protein derivative
Kenji Tadokoro - One of the best experts on this subject based on the ideXlab platform.
-
the diagnostic value of a new whole blood histamine release Test using paper disc coupled antigens results compared with Intracutaneous Test rast and eye Test
Arerugī (Allergy), 1991Co-Authors: Kenji Tadokoro, Haruhisa Mita, Kazuo Akiyama, T Hayakawa, Maki Hasegawa, Yuji Maeda, Hiroshi Yasueda, T ShidaAbstract:We have developed a novel histamine release Test (HR) using whole blood and antigen-coupled RAST paper discs, for the screening of allergens rather in a short time with a small amount of blood. Histamine was determined by a RIA kit. In order to evaluate the diagnostic value, the results of the Test were compared with those of RAST, Intracutaneous Tests, and eye Tests in 45 allergic patients. HR correlates better with RAST than Intracutaneous Test in almost all antigens. The closest positive correlation with the other Tests was seen in mite allergen, followed by pollen, foods and mould spores in that order. When the HR was positive for house dust, 100% of the patients were also positive for the eye Test, which is reported to closely correlate with bronchial provocation Tests. HR seemed to be a useful Test not only for the screening but also for the determination of pathogenic allergens.
-
topical thrombin induced ige mediated anaphylaxis rast analysis and skin Test studies
The Journal of Allergy and Clinical Immunology, 1991Co-Authors: Kenji Tadokoro, Takayuki Ohtoshi, Shigeru Takafuji, Kazunori Nakajima, Shuji Suzuki, Kazuhiko Yamamoto, Koji Ito, Terumasa Miyamoto, Masaharu MuranakaAbstract:Bovine topical thrombin (BTT) is a heterologous plasma thrombin concentrate that has been frequently used for the hemostasis since the 1940s. Recently, three patients in Japan went into shock after the topical application of BTT at lesion sites, and two of these patients had received BTT repeatedly. The clinical symptoms and the increased anti-BTT percent RAST counts suggest that these reactions were shock mediated by anti-BTT IgE antibodies. The RAST-inhibition analysis suggested that the antigenic substance(s) were bovine-specific moiety(ies) mainly involved in the contaminant rather than bovine thrombin itself. The skin Tests were studied to predict such allergic reactions. The Intracutaneous Test provoked nonspecific reactions even at the low concentrations of BTT. The prospective study on the predictive value of the prick Test with 1000 U/ml (1 mg/ml) of BTT in 192 patients suggested that it is useful to detect highly sensitive patients. In addition, the increased levels of anti-BTT IgE antibodies in patients 1 month after the single administration of BTT suggested the immunogenicity of the topical application of BTT.