The Experts below are selected from a list of 9876 Experts worldwide ranked by ideXlab platform

Swee Keong Yeap - One of the best experts on this subject based on the ideXlab platform.

  • transcriptome analysis of chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    The infectious bursal disease (IBD) is an acute immunosuppressive viral disease that significantly affects the economics of the poultry industry. The IBD virus (IBDV) was known to infect B Lymphocytes and activate macrophage and T Lymphocytes, but there are limited studies on the impact of IBDV infection on chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells. This study employed an mRNA sequencing approach to investigate the early regulation of gene expression patterns in chicken IEL-NK cells after infection with very virulent IBDV strain UPM0081. A total of 12,141 genes were expressed in uninfected chicken IEL-NK cells, and most of the genes with high expression were involved in the metabolic pathway, whereas most of the low expressed genes were involved in the cytokine-cytokine receptor pathway. A total of 1,266 genes were differentially expressed (DE) at 3 day-post-infection (dpi), and these DE genes were involved in inflammation, antiviral response and interferon stimulation. The innate immune response was activated as several genes involved in inflammation, antiviral response and recruitment of NK cells to the infected area were up-regulated. This is the first study to examine the whole transcriptome profile of chicken NK cells towards IBDV infection and provides better insight into the early immune response of chicken NK cells.

  • identification of reference genes in chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    Due to the limitations in the range of antibodies recognising avian viruses, quantitative real-time PCR (RT-qPCR) is still the most widely used method to evaluate the expression of immunologically related genes in avian viruses. The objective of this study was to identify suitable reference genes for mRNA expression analysis in chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells after infection with very-virulent infectious bursal disease virus (vvIBDV). Fifteen potential reference genes were selected based on the references available. The coefficient of variation percentage (CV%) and average count of these 15 genes were determined by NanoString technology for control and infected samples. The M and V values for shortlisted reference genes (ACTB, GAPDH, HMBS, HPRT1, SDHA, TUBB1 and YWHAZ) were calculated using geNorm and NormFinder. GAPDH, YWHAZ and HMBS were the most stably expressed genes. The expression levels of three innate immune response related target genes, CASP8, IL22 and TLR3, agreed in the NanoString and RNA sequencing (RNA-Seq) results using one or two reference genes for normalisation (not HMBS). In conclusion, GAPDH and YWHAZ could be used as reference genes for the normalisation of chicken IEL-NK cell gene responses to infection with vvIBDV.

Sook Yee Boo - One of the best experts on this subject based on the ideXlab platform.

  • transcriptome analysis of chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    The infectious bursal disease (IBD) is an acute immunosuppressive viral disease that significantly affects the economics of the poultry industry. The IBD virus (IBDV) was known to infect B Lymphocytes and activate macrophage and T Lymphocytes, but there are limited studies on the impact of IBDV infection on chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells. This study employed an mRNA sequencing approach to investigate the early regulation of gene expression patterns in chicken IEL-NK cells after infection with very virulent IBDV strain UPM0081. A total of 12,141 genes were expressed in uninfected chicken IEL-NK cells, and most of the genes with high expression were involved in the metabolic pathway, whereas most of the low expressed genes were involved in the cytokine-cytokine receptor pathway. A total of 1,266 genes were differentially expressed (DE) at 3 day-post-infection (dpi), and these DE genes were involved in inflammation, antiviral response and interferon stimulation. The innate immune response was activated as several genes involved in inflammation, antiviral response and recruitment of NK cells to the infected area were up-regulated. This is the first study to examine the whole transcriptome profile of chicken NK cells towards IBDV infection and provides better insight into the early immune response of chicken NK cells.

  • identification of reference genes in chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    Due to the limitations in the range of antibodies recognising avian viruses, quantitative real-time PCR (RT-qPCR) is still the most widely used method to evaluate the expression of immunologically related genes in avian viruses. The objective of this study was to identify suitable reference genes for mRNA expression analysis in chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells after infection with very-virulent infectious bursal disease virus (vvIBDV). Fifteen potential reference genes were selected based on the references available. The coefficient of variation percentage (CV%) and average count of these 15 genes were determined by NanoString technology for control and infected samples. The M and V values for shortlisted reference genes (ACTB, GAPDH, HMBS, HPRT1, SDHA, TUBB1 and YWHAZ) were calculated using geNorm and NormFinder. GAPDH, YWHAZ and HMBS were the most stably expressed genes. The expression levels of three innate immune response related target genes, CASP8, IL22 and TLR3, agreed in the NanoString and RNA sequencing (RNA-Seq) results using one or two reference genes for normalisation (not HMBS). In conclusion, GAPDH and YWHAZ could be used as reference genes for the normalisation of chicken IEL-NK cell gene responses to infection with vvIBDV.

Sheau Wei Tan - One of the best experts on this subject based on the ideXlab platform.

  • transcriptome analysis of chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    The infectious bursal disease (IBD) is an acute immunosuppressive viral disease that significantly affects the economics of the poultry industry. The IBD virus (IBDV) was known to infect B Lymphocytes and activate macrophage and T Lymphocytes, but there are limited studies on the impact of IBDV infection on chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells. This study employed an mRNA sequencing approach to investigate the early regulation of gene expression patterns in chicken IEL-NK cells after infection with very virulent IBDV strain UPM0081. A total of 12,141 genes were expressed in uninfected chicken IEL-NK cells, and most of the genes with high expression were involved in the metabolic pathway, whereas most of the low expressed genes were involved in the cytokine-cytokine receptor pathway. A total of 1,266 genes were differentially expressed (DE) at 3 day-post-infection (dpi), and these DE genes were involved in inflammation, antiviral response and interferon stimulation. The innate immune response was activated as several genes involved in inflammation, antiviral response and recruitment of NK cells to the infected area were up-regulated. This is the first study to examine the whole transcriptome profile of chicken NK cells towards IBDV infection and provides better insight into the early immune response of chicken NK cells.

  • identification of reference genes in chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    Due to the limitations in the range of antibodies recognising avian viruses, quantitative real-time PCR (RT-qPCR) is still the most widely used method to evaluate the expression of immunologically related genes in avian viruses. The objective of this study was to identify suitable reference genes for mRNA expression analysis in chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells after infection with very-virulent infectious bursal disease virus (vvIBDV). Fifteen potential reference genes were selected based on the references available. The coefficient of variation percentage (CV%) and average count of these 15 genes were determined by NanoString technology for control and infected samples. The M and V values for shortlisted reference genes (ACTB, GAPDH, HMBS, HPRT1, SDHA, TUBB1 and YWHAZ) were calculated using geNorm and NormFinder. GAPDH, YWHAZ and HMBS were the most stably expressed genes. The expression levels of three innate immune response related target genes, CASP8, IL22 and TLR3, agreed in the NanoString and RNA sequencing (RNA-Seq) results using one or two reference genes for normalisation (not HMBS). In conclusion, GAPDH and YWHAZ could be used as reference genes for the normalisation of chicken IEL-NK cell gene responses to infection with vvIBDV.

Noorjahan Banu Alitheen - One of the best experts on this subject based on the ideXlab platform.

  • transcriptome analysis of chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    The infectious bursal disease (IBD) is an acute immunosuppressive viral disease that significantly affects the economics of the poultry industry. The IBD virus (IBDV) was known to infect B Lymphocytes and activate macrophage and T Lymphocytes, but there are limited studies on the impact of IBDV infection on chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells. This study employed an mRNA sequencing approach to investigate the early regulation of gene expression patterns in chicken IEL-NK cells after infection with very virulent IBDV strain UPM0081. A total of 12,141 genes were expressed in uninfected chicken IEL-NK cells, and most of the genes with high expression were involved in the metabolic pathway, whereas most of the low expressed genes were involved in the cytokine-cytokine receptor pathway. A total of 1,266 genes were differentially expressed (DE) at 3 day-post-infection (dpi), and these DE genes were involved in inflammation, antiviral response and interferon stimulation. The innate immune response was activated as several genes involved in inflammation, antiviral response and recruitment of NK cells to the infected area were up-regulated. This is the first study to examine the whole transcriptome profile of chicken NK cells towards IBDV infection and provides better insight into the early immune response of chicken NK cells.

  • identification of reference genes in chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    Due to the limitations in the range of antibodies recognising avian viruses, quantitative real-time PCR (RT-qPCR) is still the most widely used method to evaluate the expression of immunologically related genes in avian viruses. The objective of this study was to identify suitable reference genes for mRNA expression analysis in chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells after infection with very-virulent infectious bursal disease virus (vvIBDV). Fifteen potential reference genes were selected based on the references available. The coefficient of variation percentage (CV%) and average count of these 15 genes were determined by NanoString technology for control and infected samples. The M and V values for shortlisted reference genes (ACTB, GAPDH, HMBS, HPRT1, SDHA, TUBB1 and YWHAZ) were calculated using geNorm and NormFinder. GAPDH, YWHAZ and HMBS were the most stably expressed genes. The expression levels of three innate immune response related target genes, CASP8, IL22 and TLR3, agreed in the NanoString and RNA sequencing (RNA-Seq) results using one or two reference genes for normalisation (not HMBS). In conclusion, GAPDH and YWHAZ could be used as reference genes for the normalisation of chicken IEL-NK cell gene responses to infection with vvIBDV.

Abdul Rahman Omar - One of the best experts on this subject based on the ideXlab platform.

  • transcriptome analysis of chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    The infectious bursal disease (IBD) is an acute immunosuppressive viral disease that significantly affects the economics of the poultry industry. The IBD virus (IBDV) was known to infect B Lymphocytes and activate macrophage and T Lymphocytes, but there are limited studies on the impact of IBDV infection on chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells. This study employed an mRNA sequencing approach to investigate the early regulation of gene expression patterns in chicken IEL-NK cells after infection with very virulent IBDV strain UPM0081. A total of 12,141 genes were expressed in uninfected chicken IEL-NK cells, and most of the genes with high expression were involved in the metabolic pathway, whereas most of the low expressed genes were involved in the cytokine-cytokine receptor pathway. A total of 1,266 genes were differentially expressed (DE) at 3 day-post-infection (dpi), and these DE genes were involved in inflammation, antiviral response and interferon stimulation. The innate immune response was activated as several genes involved in inflammation, antiviral response and recruitment of NK cells to the infected area were up-regulated. This is the first study to examine the whole transcriptome profile of chicken NK cells towards IBDV infection and provides better insight into the early immune response of chicken NK cells.

  • identification of reference genes in chicken Intraepithelial Lymphocyte natural killer cells infected with very virulent infectious bursal disease virus
    Scientific Reports, 2020
    Co-Authors: Sook Yee Boo, Sheau Wei Tan, Noorjahan Banu Alitheen, Abdul Rahman Omar, Swee Keong Yeap
    Abstract:

    Due to the limitations in the range of antibodies recognising avian viruses, quantitative real-time PCR (RT-qPCR) is still the most widely used method to evaluate the expression of immunologically related genes in avian viruses. The objective of this study was to identify suitable reference genes for mRNA expression analysis in chicken Intraepithelial Lymphocyte natural killer (IEL-NK) cells after infection with very-virulent infectious bursal disease virus (vvIBDV). Fifteen potential reference genes were selected based on the references available. The coefficient of variation percentage (CV%) and average count of these 15 genes were determined by NanoString technology for control and infected samples. The M and V values for shortlisted reference genes (ACTB, GAPDH, HMBS, HPRT1, SDHA, TUBB1 and YWHAZ) were calculated using geNorm and NormFinder. GAPDH, YWHAZ and HMBS were the most stably expressed genes. The expression levels of three innate immune response related target genes, CASP8, IL22 and TLR3, agreed in the NanoString and RNA sequencing (RNA-Seq) results using one or two reference genes for normalisation (not HMBS). In conclusion, GAPDH and YWHAZ could be used as reference genes for the normalisation of chicken IEL-NK cell gene responses to infection with vvIBDV.