The Experts below are selected from a list of 63 Experts worldwide ranked by ideXlab platform

Menglin Cong - One of the best experts on this subject based on the ideXlab platform.

  • the effects of neuregulin 1β on Intrafusal Muscle Fiber formation in neuromuscular coculture of dorsal root ganglion explants and skeletal Muscle cells
    Skeletal Muscle, 2018
    Co-Authors: Yuan Qiao, Menglin Cong
    Abstract:

    Background The formation of Intrafusal Muscle (IM) Fibers and their contact with afferent proprioceptive axons is critical for construction, function, and maintenance of the stretch reflex. Many factors affect the formation of IM Fibers. Finding new factors and mechanisms of IM Fiber formation is essential for the reconstruction of stretch reflex arc after injury.

  • The effects of neuregulin-1β on Intrafusal Muscle Fiber formation in neuromuscular coculture of dorsal root ganglion explants and skeletal Muscle cells
    BMC, 2018
    Co-Authors: Yuan Qiao, Menglin Cong
    Abstract:

    Abstract Background The formation of Intrafusal Muscle (IM) Fibers and their contact with afferent proprioceptive axons is critical for construction, function, and maintenance of the stretch reflex. Many factors affect the formation of IM Fibers. Finding new factors and mechanisms of IM Fiber formation is essential for the reconstruction of stretch reflex arc after injury. Methods We established a coculture system of organotypic dorsal root ganglion (DRG) explants and dissociated skeletal Muscle (SKM) cells. The formation of IM Fibers was observed in this coculture system after neuregulin-1β (NRG-1β) incubation. Results We found that NRG-1β promoted outgrowth of neurites and migration of neurons from the organotypic DRG explants and that this correlated with an induction of growth-associated protein 43 (GAP-43) expression. NRG-1β also increased the amount of nuclear bag Fibers and nuclear chain Fibers by elevating the proportion of tyrosine kinase receptor C (TrkC) phenotypic DRG neurons. In addition, we found that the effects of NRG-1β could be blocked by inhibiting ERK1/2, PI3K/Akt, and JAK2/STAT3 signaling pathways. Conclusion These data imply that NRG-1β promoted neurite outgrowth and neuronal migration from the organotypic DRG explants and that this correlated with an induction of GAP-43 expression. The modulating effects of NRG-1β on TrkC DRG neuronal phenotype may link to promote IM Fiber formation. The effects produced by NRG-1β in this neuromuscular coculture system provide new data for the therapeutic potential on IM Fiber formation after Muscle injury

Warren G. Tourtellotte - One of the best experts on this subject based on the ideXlab platform.

  • egr3 dependent Muscle spindle stretch receptor Intrafusal Muscle Fiber differentiation and fusimotor innervation homeostasis
    The Journal of Neuroscience, 2015
    Co-Authors: Michelle Oliveira Fernandes, Warren G. Tourtellotte
    Abstract:

    Muscle stretch proprioceptors (Muscle spindles) are required for stretch reflexes and locomotor control. Proprioception abnormalities are observed in many human neuropathies, but the mechanisms involved in establishing and maintaining Muscle spindle innervation and function are still poorly understood. During skeletal Muscle development, sensory (Ia-afferent) innervation induces contacted myotubes to transform into Intrafusal Muscle Fibers that form the stretch receptor core. The transcriptional regulator Egr3 is induced in Ia-afferent contacted myotubes by Neuregulin1 (Nrg1)/ErbB receptor signaling and it has an essential role in spindle morphogenesis and function. Because Egr3 is widely expressed during development and has a pleiotropic function, whether Egr3 functions primarily in skeletal Muscle, Ia-afferent neurons, or in Schwann cells that myelinate Ia-afferent axons remains unresolved. In the present studies, cell-specific ablation of Egr3 in mice showed that it has a skeletal Muscle autonomous function in stretch receptor development. Moreover, using genetic tracing, we found that Ia-afferent contacted Egr3-deficient myotubes were induced in normal numbers, but their development was blocked to generate one to two shortened Fibers that failed to express some characteristic myosin heavy chain (MyHC) proteins. These “spindle remnants” persisted into adulthood, remained innervated by Ia-afferents, and expressed neurotrophin3 (NT3), which is required for Ia-afferent neuron survival. However, they were not innervated by fusimotor axons and they did not express glial derived neurotrophic factor (GDNF), which is essential for fusimotor neuron survival. These results demonstrate that Egr3 has an essential role in regulating gene expression that promotes normal Intrafusal Muscle Fiber differentiation and fusimotor innervation homeostasis.

  • JCB: ARTICLE Transcriptional regulation of myotube fate specification and Intrafusal Muscle Fiber morphogenesis
    2013
    Co-Authors: Jennifer Whitehead, Xiaoguang Gao, Laurie Eldredge, John Carter, Warren G. Tourtellotte
    Abstract:

    Vertebrate Muscle spindle stretch receptors are important for limb position sensation (proprioception) and stretch reflexes. The structurally complex stretch receptor arises from a single myotube, which is transformed into multiple Intrafusal Muscle Fibers by sensory axon–dependent signal transduction that alters gene expression in the contacted myotubes. The sensory-derived signal transduction pathways that specify the fate of myotubes are very poorly understood. The zinc finger transcription factor, early growth response gene 3 (Egr3), is selectively expressed in sensory axon–contacted myotubes, and it is required for normal Intrafusal Muscle Fiber differentiation and spindle development. Here, we show that overexpression of Egr3 in primary myotubes in vitro lead

  • Transcriptional regulation of myotube fate specification and Intrafusal Muscle Fiber morphogenesis
    The Journal of cell biology, 2005
    Co-Authors: Y'vonne Albert, Jennifer Whitehead, Laurie C. Eldredge, John M. Carter, Xiaoguang Gao, Warren G. Tourtellotte
    Abstract:

    Vertebrate Muscle spindle stretch receptors are important for limb position sensation (proprioception) and stretch reflexes. The structurally complex stretch receptor arises from a single myotube, which is transformed into multiple Intrafusal Muscle Fibers by sensory axon-dependent signal transduction that alters gene expression in the contacted myotubes. The sensory-derived signal transduction pathways that specify the fate of myotubes are very poorly understood. The zinc finger transcription factor, early growth response gene 3 (Egr3), is selectively expressed in sensory axon-contacted myotubes, and it is required for normal Intrafusal Muscle Fiber differentiation and spindle development. Here, we show that overexpression of Egr3 in primary myotubes in vitro leads to the expression of a particular repertoire of genes, some of which we demonstrate are also regulated by Egr3 in developing Intrafusal Muscle Fibers within spindles. Thus, our results identify a network of genes that are regulated by Egr3 and are involved in Intrafusal Muscle Fiber differentiation. Moreover, we show that Egr3 mediates myotube fate specification that is induced by sensory innervation because skeletal myotubes that express Egr3 independent of other sensory axon regulation are transformed into Muscle Fibers with structural and molecular similarities to Intrafusal Muscle Fibers. Hence, Egr3 is a target gene that is regulated by sensory innervation and that mediates gene expression involved in myotube fate specification and Intrafusal Muscle Fiber morphogenesis.

Yuan Qiao - One of the best experts on this subject based on the ideXlab platform.

  • the effects of neuregulin 1β on Intrafusal Muscle Fiber formation in neuromuscular coculture of dorsal root ganglion explants and skeletal Muscle cells
    Skeletal Muscle, 2018
    Co-Authors: Yuan Qiao, Menglin Cong
    Abstract:

    Background The formation of Intrafusal Muscle (IM) Fibers and their contact with afferent proprioceptive axons is critical for construction, function, and maintenance of the stretch reflex. Many factors affect the formation of IM Fibers. Finding new factors and mechanisms of IM Fiber formation is essential for the reconstruction of stretch reflex arc after injury.

  • The effects of neuregulin-1β on Intrafusal Muscle Fiber formation in neuromuscular coculture of dorsal root ganglion explants and skeletal Muscle cells
    BMC, 2018
    Co-Authors: Yuan Qiao, Menglin Cong
    Abstract:

    Abstract Background The formation of Intrafusal Muscle (IM) Fibers and their contact with afferent proprioceptive axons is critical for construction, function, and maintenance of the stretch reflex. Many factors affect the formation of IM Fibers. Finding new factors and mechanisms of IM Fiber formation is essential for the reconstruction of stretch reflex arc after injury. Methods We established a coculture system of organotypic dorsal root ganglion (DRG) explants and dissociated skeletal Muscle (SKM) cells. The formation of IM Fibers was observed in this coculture system after neuregulin-1β (NRG-1β) incubation. Results We found that NRG-1β promoted outgrowth of neurites and migration of neurons from the organotypic DRG explants and that this correlated with an induction of growth-associated protein 43 (GAP-43) expression. NRG-1β also increased the amount of nuclear bag Fibers and nuclear chain Fibers by elevating the proportion of tyrosine kinase receptor C (TrkC) phenotypic DRG neurons. In addition, we found that the effects of NRG-1β could be blocked by inhibiting ERK1/2, PI3K/Akt, and JAK2/STAT3 signaling pathways. Conclusion These data imply that NRG-1β promoted neurite outgrowth and neuronal migration from the organotypic DRG explants and that this correlated with an induction of GAP-43 expression. The modulating effects of NRG-1β on TrkC DRG neuronal phenotype may link to promote IM Fiber formation. The effects produced by NRG-1β in this neuromuscular coculture system provide new data for the therapeutic potential on IM Fiber formation after Muscle injury

Y'vonne Albert - One of the best experts on this subject based on the ideXlab platform.

  • Transcriptional regulation of myotube fate specification and Intrafusal Muscle Fiber morphogenesis
    The Journal of cell biology, 2005
    Co-Authors: Y'vonne Albert, Jennifer Whitehead, Laurie C. Eldredge, John M. Carter, Xiaoguang Gao, Warren G. Tourtellotte
    Abstract:

    Vertebrate Muscle spindle stretch receptors are important for limb position sensation (proprioception) and stretch reflexes. The structurally complex stretch receptor arises from a single myotube, which is transformed into multiple Intrafusal Muscle Fibers by sensory axon-dependent signal transduction that alters gene expression in the contacted myotubes. The sensory-derived signal transduction pathways that specify the fate of myotubes are very poorly understood. The zinc finger transcription factor, early growth response gene 3 (Egr3), is selectively expressed in sensory axon-contacted myotubes, and it is required for normal Intrafusal Muscle Fiber differentiation and spindle development. Here, we show that overexpression of Egr3 in primary myotubes in vitro leads to the expression of a particular repertoire of genes, some of which we demonstrate are also regulated by Egr3 in developing Intrafusal Muscle Fibers within spindles. Thus, our results identify a network of genes that are regulated by Egr3 and are involved in Intrafusal Muscle Fiber differentiation. Moreover, we show that Egr3 mediates myotube fate specification that is induced by sensory innervation because skeletal myotubes that express Egr3 independent of other sensory axon regulation are transformed into Muscle Fibers with structural and molecular similarities to Intrafusal Muscle Fibers. Hence, Egr3 is a target gene that is regulated by sensory innervation and that mediates gene expression involved in myotube fate specification and Intrafusal Muscle Fiber morphogenesis.

Jennifer Whitehead - One of the best experts on this subject based on the ideXlab platform.

  • JCB: ARTICLE Transcriptional regulation of myotube fate specification and Intrafusal Muscle Fiber morphogenesis
    2013
    Co-Authors: Jennifer Whitehead, Xiaoguang Gao, Laurie Eldredge, John Carter, Warren G. Tourtellotte
    Abstract:

    Vertebrate Muscle spindle stretch receptors are important for limb position sensation (proprioception) and stretch reflexes. The structurally complex stretch receptor arises from a single myotube, which is transformed into multiple Intrafusal Muscle Fibers by sensory axon–dependent signal transduction that alters gene expression in the contacted myotubes. The sensory-derived signal transduction pathways that specify the fate of myotubes are very poorly understood. The zinc finger transcription factor, early growth response gene 3 (Egr3), is selectively expressed in sensory axon–contacted myotubes, and it is required for normal Intrafusal Muscle Fiber differentiation and spindle development. Here, we show that overexpression of Egr3 in primary myotubes in vitro lead

  • Transcriptional regulation of myotube fate specification and Intrafusal Muscle Fiber morphogenesis
    The Journal of cell biology, 2005
    Co-Authors: Y'vonne Albert, Jennifer Whitehead, Laurie C. Eldredge, John M. Carter, Xiaoguang Gao, Warren G. Tourtellotte
    Abstract:

    Vertebrate Muscle spindle stretch receptors are important for limb position sensation (proprioception) and stretch reflexes. The structurally complex stretch receptor arises from a single myotube, which is transformed into multiple Intrafusal Muscle Fibers by sensory axon-dependent signal transduction that alters gene expression in the contacted myotubes. The sensory-derived signal transduction pathways that specify the fate of myotubes are very poorly understood. The zinc finger transcription factor, early growth response gene 3 (Egr3), is selectively expressed in sensory axon-contacted myotubes, and it is required for normal Intrafusal Muscle Fiber differentiation and spindle development. Here, we show that overexpression of Egr3 in primary myotubes in vitro leads to the expression of a particular repertoire of genes, some of which we demonstrate are also regulated by Egr3 in developing Intrafusal Muscle Fibers within spindles. Thus, our results identify a network of genes that are regulated by Egr3 and are involved in Intrafusal Muscle Fiber differentiation. Moreover, we show that Egr3 mediates myotube fate specification that is induced by sensory innervation because skeletal myotubes that express Egr3 independent of other sensory axon regulation are transformed into Muscle Fibers with structural and molecular similarities to Intrafusal Muscle Fibers. Hence, Egr3 is a target gene that is regulated by sensory innervation and that mediates gene expression involved in myotube fate specification and Intrafusal Muscle Fiber morphogenesis.