The Experts below are selected from a list of 246 Experts worldwide ranked by ideXlab platform
Irvin S. Y. Chen - One of the best experts on this subject based on the ideXlab platform.
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Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through Intravenous Injection
Nature Medicine, 2005Co-Authors: Kouki Morizono, Yiming Xie, Mai Johnson, Hoorig Nassanian, Benhur Lee, Gene Errol Ringpis, Lily Wu, Irvin S. Y. ChenAbstract:Targeted gene transduction to specific tissues and organs through Intravenous Injection would be the ultimate preferred method of gene delivery. Here, we report successful targeting in a living animal through Intravenous Injection of a lentiviral vector pseudotyped with a modified chimeric Sindbis virus envelope (termed m168). m168 pseudotypes have high titer and high targeting specificity and, unlike other retroviral pseudotypes, have low nonspecific infectivity in liver and spleen. A mouse cancer model of metastatic melanoma was used to test Intravenous targeting with m168. Human P-glycoprotein was ectopically expressed on the surface of melanoma cells and targeted by the m168 pseudotyped lentiviral vector conjugated with antibody specific for P-glycoprotein. m168 pseudotypes successfully targeted metastatic melanoma cells growing in the lung after systemic administration by tail vein Injection. Further development of this targeting technology should result in applications not only for cancers but also for genetic, infectious and immune diseases.
Kouki Morizono - One of the best experts on this subject based on the ideXlab platform.
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Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through Intravenous Injection
Nature Medicine, 2005Co-Authors: Kouki Morizono, Yiming Xie, Mai Johnson, Hoorig Nassanian, Benhur Lee, Gene Errol Ringpis, Lily Wu, Irvin S. Y. ChenAbstract:Targeted gene transduction to specific tissues and organs through Intravenous Injection would be the ultimate preferred method of gene delivery. Here, we report successful targeting in a living animal through Intravenous Injection of a lentiviral vector pseudotyped with a modified chimeric Sindbis virus envelope (termed m168). m168 pseudotypes have high titer and high targeting specificity and, unlike other retroviral pseudotypes, have low nonspecific infectivity in liver and spleen. A mouse cancer model of metastatic melanoma was used to test Intravenous targeting with m168. Human P-glycoprotein was ectopically expressed on the surface of melanoma cells and targeted by the m168 pseudotyped lentiviral vector conjugated with antibody specific for P-glycoprotein. m168 pseudotypes successfully targeted metastatic melanoma cells growing in the lung after systemic administration by tail vein Injection. Further development of this targeting technology should result in applications not only for cancers but also for genetic, infectious and immune diseases.
Andrew P. Byrnes - One of the best experts on this subject based on the ideXlab platform.
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Rapid Kupffer cell death after Intravenous Injection of adenovirus vectors
Molecular Therapy, 2006Co-Authors: Elanchezhiyan Manickan, Jeffrey S. Smith, Jie Tian, Thomas L. Eggerman, Jay N. Lozier, Jacqueline Muller, Andrew P. ByrnesAbstract:When adenovirus vectors are injected Intravenously, they are quickly taken up by Kupffer cells in the liver. We report that this causes rapid necrosis of Kupffer cells in mice at doses of 1011 particles/kg or higher. By 10 min after Intravenous vector Injection, Kupffer cells were permeable to propidium iodide and trypan blue. This coincided with a sharp rise in serum lactate dehydrogenase. Ultrastructural examination showed degeneration of Kupffer cells, including complete disappearance of chromatin by 1 h. After an initial Intravenous Injection of vector, dead Kupffer cells were unable to take up a second dose of vector, and hepatic transgene expression from the second dose was augmented. Death of Kupffer cells did not affect serum levels of IL-6 or IL-12. There was no immediate change in the number of Kupffer cells in the liver, but a significant decline was found by 4 h after Injection of vector. Interestingly, substantial numbers of vector-containing Kupffer cells were found in pulmonary capillaries, indicating that they had been swept out of the liver. Together these results show that an Intravenous Injection of adenovirus vector causes synchronous and surprisingly rapid Kupffer cell death.
Yiming Xie - One of the best experts on this subject based on the ideXlab platform.
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Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through Intravenous Injection
Nature Medicine, 2005Co-Authors: Kouki Morizono, Yiming Xie, Mai Johnson, Hoorig Nassanian, Benhur Lee, Gene Errol Ringpis, Lily Wu, Irvin S. Y. ChenAbstract:Targeted gene transduction to specific tissues and organs through Intravenous Injection would be the ultimate preferred method of gene delivery. Here, we report successful targeting in a living animal through Intravenous Injection of a lentiviral vector pseudotyped with a modified chimeric Sindbis virus envelope (termed m168). m168 pseudotypes have high titer and high targeting specificity and, unlike other retroviral pseudotypes, have low nonspecific infectivity in liver and spleen. A mouse cancer model of metastatic melanoma was used to test Intravenous targeting with m168. Human P-glycoprotein was ectopically expressed on the surface of melanoma cells and targeted by the m168 pseudotyped lentiviral vector conjugated with antibody specific for P-glycoprotein. m168 pseudotypes successfully targeted metastatic melanoma cells growing in the lung after systemic administration by tail vein Injection. Further development of this targeting technology should result in applications not only for cancers but also for genetic, infectious and immune diseases.
Hoorig Nassanian - One of the best experts on this subject based on the ideXlab platform.
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Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through Intravenous Injection
Nature Medicine, 2005Co-Authors: Kouki Morizono, Yiming Xie, Mai Johnson, Hoorig Nassanian, Benhur Lee, Gene Errol Ringpis, Lily Wu, Irvin S. Y. ChenAbstract:Targeted gene transduction to specific tissues and organs through Intravenous Injection would be the ultimate preferred method of gene delivery. Here, we report successful targeting in a living animal through Intravenous Injection of a lentiviral vector pseudotyped with a modified chimeric Sindbis virus envelope (termed m168). m168 pseudotypes have high titer and high targeting specificity and, unlike other retroviral pseudotypes, have low nonspecific infectivity in liver and spleen. A mouse cancer model of metastatic melanoma was used to test Intravenous targeting with m168. Human P-glycoprotein was ectopically expressed on the surface of melanoma cells and targeted by the m168 pseudotyped lentiviral vector conjugated with antibody specific for P-glycoprotein. m168 pseudotypes successfully targeted metastatic melanoma cells growing in the lung after systemic administration by tail vein Injection. Further development of this targeting technology should result in applications not only for cancers but also for genetic, infectious and immune diseases.