The Experts below are selected from a list of 375 Experts worldwide ranked by ideXlab platform
Jongsang Kim - One of the best experts on this subject based on the ideXlab platform.
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improvement of memory and cognitive function by alantolactone and isoalantolactone in mouse model 629 9
The FASEB Journal, 2014Co-Authors: Ji Yeon Seo, Seong Soon Kim, Hye Ryung Kang, Bo Ram Kim, Alexandra Zakharova, Ji Eun Woo, Mi Hye Kim, Mi Kyung Han, Jongsang KimAbstract:Our previous study demonstrated that alantolactone and its isoform isolated from Inula helenium had neuroprotective and antioxidative effects in primary cortical neurons. This study was conducted t...
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isoalantolactone from Inula helenium caused nrf2 mediated induction of detoxifying enzymes
Journal of Medicinal Food, 2009Co-Authors: Ji Yeon Seo, Soon Sung Lim, Jisun Lim, In Ae Lee, Jung Han Yoon Park, Jia Park, Hyo Jung Kim, Se Jin Choi, Hui Jung Kang, Jongsang KimAbstract:Our previous study demonstrated that methanolic extract of Inula helenium (Elecampane) has the potential to induce detoxifying enzymes such as NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) (NQO1, QR) activity and glutathione S-transferase (GST) and found isoalantolactone and alantolactone as the active components. In this study we investigated the detoxifying enzyme-inducing potential of isoalantolactone, which is present in I. helenium and has a structure similar to that of alantolactone. The compound induced QR in a dose-dependent manner in both Hepa1c1c7 cells and its mutant BPRc1 cells lacking the arylhydrocarbon receptor translocator. Like with most phase 2 enzyme inducers, other phase 2 detoxifying enzymes, including GST, glutathione reductase, gamma-glutamylcysteine synthetase, and heme oxygenase-1, were also induced by isoalantolactone in a dose-dependent manner in the cultured cells. Furthermore, isoalantolactone caused a proportionate increase in luciferase activity depending upon concentration and exposure time in the reporter assay in which HepG2-C8 cells, transfectants carrying antioxidant response element-luciferase gene, were used. The nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) was stimulated by the compound and attenuated by phosphatidylinositol 3-kinase inhibitors such as LY294002 and wortmannin. In conclusion, isoalantolactone is a candidate for chemoprevention and acts as potent phase 2 enzyme inducer by stimulating the accumulation of Nrf2 in the nucleus.
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nrf2 mediated induction of detoxifying enzymes by alantolactone present in Inula helenium
Phytotherapy Research, 2008Co-Authors: Ji Yeon Seo, Soon Sung Lim, Ju Ryoung Kim, Jisun Lim, In Ae Lee, Eun Ji Kim, Jung Han Yoon Park, Jongsang KimAbstract:Our previous study showed that a methanol extract of Inula helenium had the potential to induce detoxifying enzymes such as quinone reductase (QR) and glutathione S-transferase (GST) activity. In this study the methanol extract was further fractionated using silica gel chromatography and vacuum liquid chromatography, to yield pure compounds alantolactone and isoalantolactone as QR inducers. Alantolactone caused a dose-dependent induction of antioxidant enzymes including QR, GST, γ-glutamylcysteine synthase, glutathione reductase, and heme oxygenase 1 in hepa1c1c7 mouse hepatoma cells. The compound increased the luciferase activity of HepG2-C8 cells, transfectants carrying antioxidant response element (ARE)-luciferase gene, in a dose-dependent manner, suggesting ARE-mediated transcriptional activation of antioxidant enzymes. Alantolactone also stimulated the nuclear accumulation of Nrf2 that was inhibited by phosphatidylinositol 3-kinase (PI3K) inhibitors. In conclusion, alantolactone appears to induce detoxifying enzymes via activation of PI3K and JNK signaling pathways, leading to translocation of Nrf2, and subsequent interaction between Nrf2 and ARE in the encoding genes. Copyright © 2008 John Wiley & Sons, Ltd.
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induction of detoxifying enzyme by sesquiterpenes present in Inula helenium
Journal of Medicinal Food, 2007Co-Authors: Soon Sung Lim, Ju Ryoung Kim, Eun Ji Kim, Jung Han Yoon Park, Hyun Ae Lim, Chan Ho Jang, Young Kyoon Kim, T Konishi, Jongsang KimAbstract:Our previous study showed that the methanolic extract of Inula helenium (elecampane) had the potential to induce detoxifying enzymes such as quinine reductase (QR) and glutathione S-transferase. In this study we further fractionated the methanolic extract into hexane-, dichloromethane-, butanol-, and water-soluble fractions according to polarity. The hexane fraction showed the highest QR-inducing activity and also induced glutathione S-transferase in a dose-dependent manner. Its potential to induce the reporter activity suggested an antioxidant response element-mediated mechanism of action in the induction of phase II detoxifying enzymes. Intraperitoneal injection of the hexane fraction of I. helenium into ICR mice caused a significant increase of QR activity in liver, kidney, small intestine, and stomach. Sesquiterpenes, isolated from the hexane fraction, appeared to be major components responsible for QR induction. Among the seven compounds tested in this study, alantolactone, isoalantolactone, and 5α-e...
Ji Yeon Seo - One of the best experts on this subject based on the ideXlab platform.
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improvement of memory and cognitive function by alantolactone and isoalantolactone in mouse model 629 9
The FASEB Journal, 2014Co-Authors: Ji Yeon Seo, Seong Soon Kim, Hye Ryung Kang, Bo Ram Kim, Alexandra Zakharova, Ji Eun Woo, Mi Hye Kim, Mi Kyung Han, Jongsang KimAbstract:Our previous study demonstrated that alantolactone and its isoform isolated from Inula helenium had neuroprotective and antioxidative effects in primary cortical neurons. This study was conducted t...
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isoalantolactone from Inula helenium caused nrf2 mediated induction of detoxifying enzymes
Journal of Medicinal Food, 2009Co-Authors: Ji Yeon Seo, Soon Sung Lim, Jisun Lim, In Ae Lee, Jung Han Yoon Park, Jia Park, Hyo Jung Kim, Se Jin Choi, Hui Jung Kang, Jongsang KimAbstract:Our previous study demonstrated that methanolic extract of Inula helenium (Elecampane) has the potential to induce detoxifying enzymes such as NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) (NQO1, QR) activity and glutathione S-transferase (GST) and found isoalantolactone and alantolactone as the active components. In this study we investigated the detoxifying enzyme-inducing potential of isoalantolactone, which is present in I. helenium and has a structure similar to that of alantolactone. The compound induced QR in a dose-dependent manner in both Hepa1c1c7 cells and its mutant BPRc1 cells lacking the arylhydrocarbon receptor translocator. Like with most phase 2 enzyme inducers, other phase 2 detoxifying enzymes, including GST, glutathione reductase, gamma-glutamylcysteine synthetase, and heme oxygenase-1, were also induced by isoalantolactone in a dose-dependent manner in the cultured cells. Furthermore, isoalantolactone caused a proportionate increase in luciferase activity depending upon concentration and exposure time in the reporter assay in which HepG2-C8 cells, transfectants carrying antioxidant response element-luciferase gene, were used. The nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) was stimulated by the compound and attenuated by phosphatidylinositol 3-kinase inhibitors such as LY294002 and wortmannin. In conclusion, isoalantolactone is a candidate for chemoprevention and acts as potent phase 2 enzyme inducer by stimulating the accumulation of Nrf2 in the nucleus.
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nrf2 mediated induction of detoxifying enzymes by alantolactone present in Inula helenium
Phytotherapy Research, 2008Co-Authors: Ji Yeon Seo, Soon Sung Lim, Ju Ryoung Kim, Jisun Lim, In Ae Lee, Eun Ji Kim, Jung Han Yoon Park, Jongsang KimAbstract:Our previous study showed that a methanol extract of Inula helenium had the potential to induce detoxifying enzymes such as quinone reductase (QR) and glutathione S-transferase (GST) activity. In this study the methanol extract was further fractionated using silica gel chromatography and vacuum liquid chromatography, to yield pure compounds alantolactone and isoalantolactone as QR inducers. Alantolactone caused a dose-dependent induction of antioxidant enzymes including QR, GST, γ-glutamylcysteine synthase, glutathione reductase, and heme oxygenase 1 in hepa1c1c7 mouse hepatoma cells. The compound increased the luciferase activity of HepG2-C8 cells, transfectants carrying antioxidant response element (ARE)-luciferase gene, in a dose-dependent manner, suggesting ARE-mediated transcriptional activation of antioxidant enzymes. Alantolactone also stimulated the nuclear accumulation of Nrf2 that was inhibited by phosphatidylinositol 3-kinase (PI3K) inhibitors. In conclusion, alantolactone appears to induce detoxifying enzymes via activation of PI3K and JNK signaling pathways, leading to translocation of Nrf2, and subsequent interaction between Nrf2 and ARE in the encoding genes. Copyright © 2008 John Wiley & Sons, Ltd.
Renyi Qin - One of the best experts on this subject based on the ideXlab platform.
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alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy lysosome pathway via targeting tfeb
Toxicology and Applied Pharmacology, 2018Co-Authors: Xiuhui Shi, Min Zhou, Yan Zhao, Chunle Zhao, Min Wang, Shutao Pan, Xingjun Guo, Renyi QinAbstract:The lysosome is emerging as a central regulator of the autophagic process, which plays a critical role in tumor growth and chemoresistance. Alantolactone, which is a natural compound produced by Inula helenium, has been shown to induce apoptosis in numerous cancer types. However, the mechanism by which alantolactone regulates apoptosis is still poorly understood. In this work, we observed that alantolactone caused the accumulation of autophagosomes due to impaired autophagic degradation and substantially inhibited the activity and expression of CTSB/CTSD proteins that when depleted caused lysosomal dysfunction. Furthermore, we found that alantolactone inhibited the proliferation of pancreatic cancer cells in vitro and in vivo and enhanced the chemosensitivity of pancreatic cancer cells to oxaliplatin. In addition, a reduction in TFEB levels was a critical event in the apoptosis and cell death caused by alantolactone. Our data demonstrated that alantolactone, which impaired autophagic degradation, was a pharmacological inhibitor of autophagy in pancreatic cancer cells and markedly enhanced the chemosensitivity of pancreatic cancer cells to oxaliplatin.
Xiang Li - One of the best experts on this subject based on the ideXlab platform.
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alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy lysosome pathway via targeting tfeb
Toxicology and Applied Pharmacology, 2018Co-Authors: Ruizhi He, Min Zhou, Yan Zhao, Chunle Zhao, Min Wang, Xiang LiAbstract:Abstract The lysosome is emerging as a central regulator of the autophagic process, which plays a critical role in tumor growth and chemoresistance. Alantolactone, which is a natural compound produced by Inula helenium, has been shown to induce apoptosis in numerous cancer types. However, the mechanism by which alantolactone regulates apoptosis is still poorly understood. In this work, we observed that alantolactone caused the accumulation of autophagosomes due to impaired autophagic degradation and substantially inhibited the activity and expression of CTSB/CTSD proteins that when depleted caused lysosomal dysfunction. Furthermore, we found that alantolactone inhibited the proliferation of pancreatic cancer cells in vitro and in vivo and enhanced the chemosensitivity of pancreatic cancer cells to oxaliplatin. In addition, a reduction in TFEB levels was a critical event in the apoptosis and cell death caused by alantolactone. Our data demonstrated that alantolactone, which impaired autophagic degradation, was a pharmacological inhibitor of autophagy in pancreatic cancer cells and markedly enhanced the chemosensitivity of pancreatic cancer cells to oxaliplatin.
Weidong Zhang - One of the best experts on this subject based on the ideXlab platform.
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total sesquiterpene lactones prepared from Inula helenium l has potentials in prevention and therapy of rheumatoid arthritis
Journal of Ethnopharmacology, 2017Co-Authors: Shuang Gao, Qun Wang, Xinhui Tian, Yunheng Shen, Weidong ZhangAbstract:Abstract Backgrounds Inula helenium L. is an herb with anti-inflammatory properties. Sesquiterpene lactones (SLs), mainly alantolactone (AL) and isoalantolactone (IAL), are considered as its active ingredients. However, the anti-inflammatory effects of SL-containing extracts of I. helenium have not been explored. Here we prepared total SLs from I. helenium (TSL-IHL), analyzed its chemical constituents, and performed cellular and animal studies to evaluate its anti-inflammatory activities. Materials and methods The chemical profile of TSL-IHL was analyzed by HPLC-UV. Its in vitro effects on the activation of signaling pathways and expression of inflammatory genes were examined by western blotting and quantitative real-time PCR, respectively, and compared with those of AL and IAL. Its in vivo anti-inflammatory effects were evaluated in adjuvant- and collagen-induced arthritis rat models. Results Chemical analysis showed that AL and IAL represent major constituents of TSL-IHL. TSL-IHL, as well as AL and IAL, could inhibit TNF- α -induced activation of NF- κ B and MAPK pathways in b. End3 cells, suppress the expressions of MMP-3, MCP-1, and IL-1 in TNF- α -stimulated synovial fibroblasts, and IL-1, IL-6, and iNOS in LPS-activated RAW 264.7 cells in a dose-dependent manner in the range of 0.6–2.4 μg/mL. Oral administration of TSL-IHL at 12.5−50 mg/kg could dose-dependently alleviate the arthritic severity and paw swelling in either developing or developed phases of arthritis of rats induced by adjuvant or collagen Conclusions These results indicated potentials of TSL-IHL in prevention and therapy of rheumatoid arthritis.
