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Rajib Lochan Goswamee - One of the best experts on this subject based on the ideXlab platform.
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harmful weed to prospective adsorbent low temperature carbonized Ipomoea carnea stem carbon coated with aluminum oxyhydroxide nanoparticles for defluoridation
Environmental Science and Pollution Research, 2019Co-Authors: Jitu Saikia, Susmita Sarmah, Pinky Saikia, Rajib Lochan GoswameeAbstract:Gainful utilization of stems of the pernicious weed, Ipomoea carnea, to prepare good quality carbon and its modification with aluminum oxyhydroxide (AlOOH) nanoparticles for efficient defluoridation from contaminated drinking water is discussed in this paper. Surface functional groups are enhanced by functionalization of the carbons under acid treatment which acted as anchor to the AlOOH nanoparticles. Formation of AlOOH particles over the carbon surface is confirmed from X-ray diffractometry analysis. The AlOOH–carbon nanocomposite showed higher fluoride removal capacity than the neat AlOOH nanoparticles with a maximum removal capacity in the range of 46.55–53.71 mg g−1. Reaction kinetics and isotherm studies showed that fluoride adsorption is quite feasible on the adsorbent surface. The column study showed the possibility of the adsorbent for large-scale applications. The adsorbent can be regenerated by a mild treatment with 0.1 N NaOH solutions. The adsorbent is highly capable for defluoridation from synthetic as well as fluoride-contaminated natural water and, thus, can be used as an alternative for commercial defluoridation adsorbents. The use of Ipomoea carnea for defluoridation can be a way of producing low-cost adsorbent material, and the use for such purposes may also be helpful to control the weed up to a good extent.
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reductant free synthesis of silver carbon nanocomposite using low temperature carbonized Ipomoea carnea stem carbon and study of its antibacterial property
Journal of environmental chemical engineering, 2018Co-Authors: Jitu Saikia, Susmita Sarmah, Rajib Lochan Goswamee, Nguadi Blaise Allou, Palakshi Bordoloi, Champa GogoiAbstract:Abstract Use of environmentally benign routes toward green synthesis of materials for pollution control is very important in recent times. This work reports one step reductant free novel approach for deposition of stabilized silver nano particles using Ipomoea carnea weed derived low temperature carbonized carbon and its prospective application towards bacteria inhibition. Low temperature carbonized Ipomoea stem carbon worked as support as well as reductant for rapid synthesis of silver nanoparticles in a very mild conditions using water as solvent in room temperature (23 °C). Reduction of silver salt to metallic silver was confirmed from x-ray diffractometry. FESEM, HRTEM images showed formation of well dispersed silver nanocrystals on the planar carbon surface. Successful, gram scale synthesis of the material was also carried out by this method. Enormous hydroxyl groups generated on low temperature carbonization in the carbon surface pushes the reaction to form silver nanoparticles. The bacteria growth inhibition test was used to evaluate the antibacterial property of the materials against Escherichia Coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus Subtilis and also tested in real pond water condition. The free radical scavenging activity of the nanocomposite was observed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Our approach of using harmful weed Ipomoea carnea to obtain valuable materials will not only help in value addition also it may be a strategy to control the weed upto some extent.
Jitu Saikia - One of the best experts on this subject based on the ideXlab platform.
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harmful weed to prospective adsorbent low temperature carbonized Ipomoea carnea stem carbon coated with aluminum oxyhydroxide nanoparticles for defluoridation
Environmental Science and Pollution Research, 2019Co-Authors: Jitu Saikia, Susmita Sarmah, Pinky Saikia, Rajib Lochan GoswameeAbstract:Gainful utilization of stems of the pernicious weed, Ipomoea carnea, to prepare good quality carbon and its modification with aluminum oxyhydroxide (AlOOH) nanoparticles for efficient defluoridation from contaminated drinking water is discussed in this paper. Surface functional groups are enhanced by functionalization of the carbons under acid treatment which acted as anchor to the AlOOH nanoparticles. Formation of AlOOH particles over the carbon surface is confirmed from X-ray diffractometry analysis. The AlOOH–carbon nanocomposite showed higher fluoride removal capacity than the neat AlOOH nanoparticles with a maximum removal capacity in the range of 46.55–53.71 mg g−1. Reaction kinetics and isotherm studies showed that fluoride adsorption is quite feasible on the adsorbent surface. The column study showed the possibility of the adsorbent for large-scale applications. The adsorbent can be regenerated by a mild treatment with 0.1 N NaOH solutions. The adsorbent is highly capable for defluoridation from synthetic as well as fluoride-contaminated natural water and, thus, can be used as an alternative for commercial defluoridation adsorbents. The use of Ipomoea carnea for defluoridation can be a way of producing low-cost adsorbent material, and the use for such purposes may also be helpful to control the weed up to a good extent.
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reductant free synthesis of silver carbon nanocomposite using low temperature carbonized Ipomoea carnea stem carbon and study of its antibacterial property
Journal of environmental chemical engineering, 2018Co-Authors: Jitu Saikia, Susmita Sarmah, Rajib Lochan Goswamee, Nguadi Blaise Allou, Palakshi Bordoloi, Champa GogoiAbstract:Abstract Use of environmentally benign routes toward green synthesis of materials for pollution control is very important in recent times. This work reports one step reductant free novel approach for deposition of stabilized silver nano particles using Ipomoea carnea weed derived low temperature carbonized carbon and its prospective application towards bacteria inhibition. Low temperature carbonized Ipomoea stem carbon worked as support as well as reductant for rapid synthesis of silver nanoparticles in a very mild conditions using water as solvent in room temperature (23 °C). Reduction of silver salt to metallic silver was confirmed from x-ray diffractometry. FESEM, HRTEM images showed formation of well dispersed silver nanocrystals on the planar carbon surface. Successful, gram scale synthesis of the material was also carried out by this method. Enormous hydroxyl groups generated on low temperature carbonization in the carbon surface pushes the reaction to form silver nanoparticles. The bacteria growth inhibition test was used to evaluate the antibacterial property of the materials against Escherichia Coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus Subtilis and also tested in real pond water condition. The free radical scavenging activity of the nanocomposite was observed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Our approach of using harmful weed Ipomoea carnea to obtain valuable materials will not only help in value addition also it may be a strategy to control the weed upto some extent.
Susmita Sarmah - One of the best experts on this subject based on the ideXlab platform.
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harmful weed to prospective adsorbent low temperature carbonized Ipomoea carnea stem carbon coated with aluminum oxyhydroxide nanoparticles for defluoridation
Environmental Science and Pollution Research, 2019Co-Authors: Jitu Saikia, Susmita Sarmah, Pinky Saikia, Rajib Lochan GoswameeAbstract:Gainful utilization of stems of the pernicious weed, Ipomoea carnea, to prepare good quality carbon and its modification with aluminum oxyhydroxide (AlOOH) nanoparticles for efficient defluoridation from contaminated drinking water is discussed in this paper. Surface functional groups are enhanced by functionalization of the carbons under acid treatment which acted as anchor to the AlOOH nanoparticles. Formation of AlOOH particles over the carbon surface is confirmed from X-ray diffractometry analysis. The AlOOH–carbon nanocomposite showed higher fluoride removal capacity than the neat AlOOH nanoparticles with a maximum removal capacity in the range of 46.55–53.71 mg g−1. Reaction kinetics and isotherm studies showed that fluoride adsorption is quite feasible on the adsorbent surface. The column study showed the possibility of the adsorbent for large-scale applications. The adsorbent can be regenerated by a mild treatment with 0.1 N NaOH solutions. The adsorbent is highly capable for defluoridation from synthetic as well as fluoride-contaminated natural water and, thus, can be used as an alternative for commercial defluoridation adsorbents. The use of Ipomoea carnea for defluoridation can be a way of producing low-cost adsorbent material, and the use for such purposes may also be helpful to control the weed up to a good extent.
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reductant free synthesis of silver carbon nanocomposite using low temperature carbonized Ipomoea carnea stem carbon and study of its antibacterial property
Journal of environmental chemical engineering, 2018Co-Authors: Jitu Saikia, Susmita Sarmah, Rajib Lochan Goswamee, Nguadi Blaise Allou, Palakshi Bordoloi, Champa GogoiAbstract:Abstract Use of environmentally benign routes toward green synthesis of materials for pollution control is very important in recent times. This work reports one step reductant free novel approach for deposition of stabilized silver nano particles using Ipomoea carnea weed derived low temperature carbonized carbon and its prospective application towards bacteria inhibition. Low temperature carbonized Ipomoea stem carbon worked as support as well as reductant for rapid synthesis of silver nanoparticles in a very mild conditions using water as solvent in room temperature (23 °C). Reduction of silver salt to metallic silver was confirmed from x-ray diffractometry. FESEM, HRTEM images showed formation of well dispersed silver nanocrystals on the planar carbon surface. Successful, gram scale synthesis of the material was also carried out by this method. Enormous hydroxyl groups generated on low temperature carbonization in the carbon surface pushes the reaction to form silver nanoparticles. The bacteria growth inhibition test was used to evaluate the antibacterial property of the materials against Escherichia Coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus Subtilis and also tested in real pond water condition. The free radical scavenging activity of the nanocomposite was observed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Our approach of using harmful weed Ipomoea carnea to obtain valuable materials will not only help in value addition also it may be a strategy to control the weed upto some extent.
Silvana Lima Gorniak - One of the best experts on this subject based on the ideXlab platform.
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maternal ingestion of Ipomoea carnea effects on goat kid bonding and behavior
Toxins, 2016Co-Authors: Andre Tadeu Gotardo, James A Pfister, P C Raspantini, Silvana Lima GorniakAbstract:Ipomoea carnea is a toxic plant found in Brazil and other tropical and subtropical countries and often causes poisoning of livestock. The plant contains the alkaloids swainsonine and calystegines, which inhibit key cellular enzymes and cause systematic cell death. This study evaluated the behavioral effects of prenatal ingestion of this plant on dams and their kids. Twenty-four pregnant goats were randomly allocated into four treatment groups and received the following doses (g/kg BW) of fresh I. carnea: 0 (control group), 1.0 (IC1), 3.0 (IC3), and 5.0 (IC5) from day 27 of gestation until parturition. Dam and kid bonding and behavior were evaluated by several tests, immediately after birth until six weeks of age. Dams from IC3 and IC5 groups spent less time paying attention to the newborn. There was a lack of maternal-infant bonding due to I. carnea intoxication. Kids from treated dams had difficulty in standing, suckling, and in recognizing their mother hours after birth. I. carnea can also compromise the kids’ ability to learn and to retain spatial memory. We suggest that kids from pregnant goats given I. carnea during gestation have significant behavioral alterations and developmental delays that may compromise their survival.
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the use of ultrasonography to study teratogenicity in ruminants evaluation of Ipomoea carnea in goats
Birth Defects Research Part B-developmental and Reproductive Toxicology, 2012Co-Authors: Andre Tadeu Gotardo, James A Pfister, Helenice De Souza Spinosa, Breno H Schumaher, Anneliese De Souza Traldi, Paulo Cesar Maiorka, Silvana Lima GorniakAbstract:Background: Ipomoea carnea (I. carnea) is a poisonous plant found in Brazil and other tropical countries that often poison livestock. The plant contains the alkaloids calystegines and mainly swainsonine, which inhibit cellular enzymes and cause systematic cell death. The objective of this study was to evaluate the perinatal effects of I. carnea in goats. Methods: Forty-seven pregnant goats were randomly allocated into 5 treatment groups and given the following doses (g/kg BW) of I. carnea: 0 (IC0), 1.0 (IC1), 3.0 (IC3), 5.0 (IC5) and 7.5 (IC7). The treatment animals were given fresh I. carnea from day 27 of gestation to parturition. Weight gains and serum biochemistry were evaluated. Fetuses were evaluated using ultrasonographic measurements. Results: Goats from the IC7 group showed clinical signs of poisoning. Ultrasound examination revealed that I. carnea feeding in all treatment groups reduced fetal movement compared to the controls. There was an increase in the total number of birth defects (retrognathia and arthrogyposis) in the IC7 and IC5 groups compared to the controls. Conclusion: The results show that I. carnea has teratogenic potential in goats. In addition, ultrasounds were useful in evaluating fetotoxicity and teratogenicity. Birth Defects Res (Part B) 00:1–7, 2012. © 2012 Wiley Periodicals, Inc.
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the immunomodulatory effects of Ipomoea carnea in rats vary depending on life stage
Human & Experimental Toxicology, 2011Co-Authors: I M Hueza, Silvana Lima GorniakAbstract:Ipomoea carnea Jacq. ssp. fistulosa (Mart. Ex Choisy; Convolvulaceae; I. carnea) possesses a toxic component: an indolizidine alkaloid swainsonine (SW) that has immunomodulatory effects due to its ...
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efeitos embriotoxicos do tratamento pre natal com extrato aquoso de Ipomoea carnea em ratos
Brazilian Journal of Veterinary Research and Animal Science, 2008Co-Authors: Rosana Zoriki Hosomi, Silvana Lima Gorniak, Helenice De Souza Spinosa, Soraya Ferreira Habr, Sandra Witaker Penteado, Franci Mary Fantinato Varoli, Maria Martha BernardiAbstract:The embryotoxic effects of prenatal daily exposure to 0.0, 0.7, 3.0 or 15.0 mg/kg of the aqueous extract (AQE) from Ipomoea carnea (I. carnea) dried leaves on gestational days 521 were studied in rats. Maternal reproductive performance, skeletal and visceral abnormalities, and malformations were evaluated. Moreover, anatomopathological findings in dams following the treatment were recorded. Regarding the dams, our results show that body weight, weight gain, food and water consumption, and reproductive performance were all unaffected by exposure to the different doses of the AQE. Nonetheless, dams treated with AQE presented a dose-dependent cytoplasmic vacuolation in the liver, kidneys, thyroid and adrenal glands. Fetal examination did not show external abnormalities or malformations. Evidences of several skeletal and visceral abnormalities were found, particularly after the higher dose of AQE. A reduced ossification centers were also detected. The present data show that prenatal ingestion of the I. carnea AQE in rats induces embryotoxicity. These effects are attributed to an active principle from I. carnea acting on maternal homeostasis, or directly in the conception.
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the role of alkaloids in Ipomoea carnea toxicosis a study in rats
Experimental and Toxicologic Pathology, 2005Co-Authors: I M Hueza, Mitsue Haraguchi, Naoki Asano, Jose Luiz Guerra, Silvana Lima GorniakAbstract:Ipomoea carnea promotes in livestock a toxicosis histologically characterized by vacuolated cells in different organs. The toxic principles of I. carnea are the alkaloids swainsonine and calystegines B1, B2, B3 and C1. However, it has not been determined whether the effects observed in rats treated with this plant are only due to swainsonine or if the calystegines have some additive toxic effect. Thus, the aim of the present study was to evaluate in rats the toxic effects of the I. carnea aqueous fraction (AF) and of its different alkaloids when administered individually at the same concentration as in this fraction, for 14 days. No anorexic effect and/or alteration in body weight was observed in any group. The histopathologic study showed that while calystegines did not produce any toxic effects, swainsonine and I. carnea AF promoted vacuolation in different organs, being more severe in the animals from the I. carnea AF group and extensible to other organs evaluated. No alterations were detected in the central nervous system of rats of any group assayed. The results obtained here suggest that calystegines may act as coadjuvants of swainsonine in I. carnea toxicosis; however, little can be proposed about the neurotoxic effect of I. carnea since rats did not prove to be a good model for the reproduction of neuronal storage disease.
Medicherla V Jagannadham - One of the best experts on this subject based on the ideXlab platform.
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purification and characterization of a new chitinase from latex of Ipomoea carnea
Process Biochemistry, 2010Co-Authors: Ashok Kumar Patel, Arthur J G Moir, Vijay K. Singh, Ravi Prakash Yadav, Medicherla V JagannadhamAbstract:Abstract A novel enzyme with endochitinase/lysozyme activity was purified to homogeneity from latex of Ipomoea carnea subsp. fistulosa using latex collection, gum removal, ammonium sulphate precipitation, hydrophobic interaction, and anion exchange chromatography. The enzyme was glycosylated (5–6%) and homogeneous on SDS-PAGE; has a molecular mass of 30.06 kDa (MALDI-TOF) and an isoelectric point of pH 4.6. The enzyme exhibited chitinase activity for hydrolysis of glycol chitin and the chitinolytic activity was significantly inhibited by allosamidin and mercuric chloride. The enzyme is stable in the pH range of 4.0–9.5, 80 °C and the optimal activity was observed at pH 5.5 and 50 °C. The enzyme consists of 8 tryptophan, 14 tyrosine, 6 cysteine residues forming three disulfide bridges and the extinction coefficient was estimated as 21.35 M −1 cm −1 . The polyclonal antibody was raised in rabbit and immunodiffusion suggests that the antigenic determinants are unique. The first 15 N-terminal residues G-E-I-T-I-Y-W-G-Q-N-G-F-E-G-S exhibited high identity to other known plant chitinases. Owing to the economic purification, high yield, unique and extraordinary features, stability and behavior; the enzyme ICChII can be widely employed in agriculture, industry, environmental protection, and in recycling chitinous waste from arthropod shellfish and for chito-oligosaccharide production.
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icchi a glycosylated chitinase from the latex of Ipomoea carnea
Phytochemistry, 2009Co-Authors: Ashok Kumar Patel, Arthur J G Moir, Vijay K. Singh, Ravi Prakash Yadav, Medicherla V JagannadhamAbstract:A multi-functional enzyme ICChI with chitinase/lysozyme/exochitinase activity from the latex of Ipomoea carnea subsp. fistulosa was purified to homogeneity using ammonium sulphate precipitation, hydrophobic interaction and size exclusion chromatography. The enzyme is glycosylated (14–15%), has a molecular mass of 34.94 kDa (MALDI–TOF) and an isoelectric point of pH 5.3. The enzyme is stable in pH range 5.0– 9.0, 80 C and the optimal activity is observed at pH 6.0 and 60 C. Using p-nitrophenyl-N-acetyl-b-D-glucosaminide, the kinetic parameters Km, Vmax, Kcat and specificity constant of the enzyme were calculated as 0.5 mM, 2.5 � 10
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crystallization and preliminary x ray analysis of carnein a serine protease from Ipomoea carnea
Acta Crystallographica Section F-structural Biology and Crystallization Communications, 2009Co-Authors: Ashok Kumar Patel, Vijay K. Singh, Medicherla V Jagannadham, Niels Van Oosterwijk, Henriette J Rozeboom, K H Kalk, Roland J Siezen, Bauke W DijkstraAbstract:Carnein is an 80 kDa subtilisin-like serine protease from the latex of the plant Ipomoea carnea which displays an exceptional resistance to chemical and thermal denaturation. In order to obtain the first crystal structure of a plant subtilisin and to gain insight into the structural determinants underlying its remarkable stability, carnein was isolated from I. carnea latex, purified and crystallized by the hanging-drop vapour-diffusion method. A data set was collected to 2.0 A resolution in-house from a single crystal at 110 K. The crystals belonged to the trigonal space group P3121 or P3221, with unit-cell parameters a = b = 126.9, c = 84.6 A, α = β = 90, γ = 120°. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient is 2.46 A3 Da−1, corresponding to a solvent content of 50%. Structure determination of the enzyme is in progress.
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Crystallization and preliminary X-ray analysis of carnein, a serine protease from Ipomoea carnea.
Acta crystallographica. Section F Structural biology and crystallization communications, 2009Co-Authors: Ashok Kumar Patel, Medicherla V Jagannadham, Niels Van Oosterwijk, Henriette J Rozeboom, K H Kalk, Roland J Siezen, Vijay Kumar Singh, Bauke W DijkstraAbstract:Carnein is an 80 kDa subtilisin-like serine protease from the latex of the plant Ipomoea carnea which displays an exceptional resistance to chemical and thermal denaturation. In order to obtain the first crystal structure of a plant subtilisin and to gain insight into the structural determinants underlying its remarkable stability, carnein was isolated from I. carnea latex, purified and crystallized by the hanging-drop vapour-diffusion method. A data set was collected to 2.0 A resolution in-house from a single crystal at 110 K. The crystals belonged to the trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 126.9, c = 84.6 A, alpha = beta = 90, gamma = 120 degrees. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient is 2.46 A(3) Da(-1), corresponding to a solvent content of 50%. Structure determination of the enzyme is in progress.
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biochemical and spectroscopic characterization of morning glory peroxidase from an invasive and hallucinogenic plant weed Ipomoea carnea
Journal of Agricultural and Food Chemistry, 2008Co-Authors: Ashok Kumar Patel, Arthur J G Moir, Vijay K. Singh, Medicherla V JagannadhamAbstract:A novel heme peroxidase MGP from the latex of Ipomoea carnea subsp. fistulosa (morning glory) belonging to the Convolvulaceae family was purified to homogeneity using ammonium sulfate precipitation, anion exchange, hydrophobic interaction, and gel filtration chromatography. The enzyme is glycosylated and has a molecular mass of 42.06 kDa (MALDI-TOF) and an isoelectric point of pH 4.3. The enzyme has high yield, broad substrate specificity, and a high stability toward pH, temperature, chaotrophs, and organic solvents. The extinction coefficient (e280 1%) of the enzyme was estimated as 20.56 and it consists of 13 tryptophan, 9 tyrosine, and 8 cysteine residues forming 4 disulfide bridges. There is significant effect of inhibitors targeting S−S bridges (mercaptoethanol, l-cysteine, glutathione), as well as of inhibitors targeting heme (sodium azide and hydroxylamine) on peroxidase activity, whereas inhibition was not observed with ethylmaleinimide due to the absence of reduced cysteine in the enzyme. Polyclo...
