The Experts below are selected from a list of 771 Experts worldwide ranked by ideXlab platform
Hiromu Kameoka - One of the best experts on this subject based on the ideXlab platform.
-
partial suppression of sos inducing activity of furylfuramide by dibasic acids from Ipomoea Nil in the salmonella typhimurium ta1535 psk1002 umu test
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Mitsuo Miyazawa, Hideo Shimamura, Sei-ichi Nakamura, Hiromu KameokaAbstract:An acidic fraction from Ipomoea Nil showed suppression of SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in the Salmonella typhimurium TA1535/pSK1002 umu test. The suppressive compounds in I. Nil were fractionated by SiO 2 column chromatography. The active compounds in the suppressing fraction were identified as butanedioic acid, pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, nonanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid, hexadecanedioic acid, 3,4-dimethoxycinnamic acid, and linoleic acid by GC and GC-MS. These dibasic acids partially suppressed the SOS-inducing activity of AF-2. The dose-response values of all dibasic acids were in the range 0.3-1.5 μmol/mL. Tetradecanedioic acid suppressed 70% of the SOS-inducing activity of AF-2 under 1.5 μmol/mL. In addition tetradecanedioic acid was assayed with other mutagens (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]indole (Glu-P-1)), and it also showed a suppressive effect on each mutagen. The ID 50 (50% inhibitory dose) values of tetradecanedioic acid for AF-2, Trp-P-1, and Glu-P-1 were estimated as 0.44, 0.80, and 0.65 μmol/mL, respectively
-
Partial Suppression of SOS-Inducing Activity of Furylfuramide by Dibasic Acids from Ipomoea Nil in the Salmonella typhimurium TA1535/pSK1002 umu Test
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Mitsuo Miyazawa, Hideo Shimamura, Sei-ichi Nakamura, Hiromu KameokaAbstract:An acidic fraction from Ipomoea Nil showed suppression of SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in the Salmonella typhimurium TA1535/pSK1002 umu test. The suppressive compounds in I. Nil were fractionated by SiO 2 column chromatography. The active compounds in the suppressing fraction were identified as butanedioic acid, pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, nonanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid, hexadecanedioic acid, 3,4-dimethoxycinnamic acid, and linoleic acid by GC and GC-MS. These dibasic acids partially suppressed the SOS-inducing activity of AF-2. The dose-response values of all dibasic acids were in the range 0.3-1.5 μmol/mL. Tetradecanedioic acid suppressed 70% of the SOS-inducing activity of AF-2 under 1.5 μmol/mL. In addition tetradecanedioic acid was assayed with other mutagens (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]indole (Glu-P-1)), and it also showed a suppressive effect on each mutagen. The ID 50 (50% inhibitory dose) values of tetradecanedioic acid for AF-2, Trp-P-1, and Glu-P-1 were estimated as 0.44, 0.80, and 0.65 μmol/mL, respectively
Mitsuo Miyazawa - One of the best experts on this subject based on the ideXlab platform.
-
partial suppression of sos inducing activity of furylfuramide by dibasic acids from Ipomoea Nil in the salmonella typhimurium ta1535 psk1002 umu test
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Mitsuo Miyazawa, Hideo Shimamura, Sei-ichi Nakamura, Hiromu KameokaAbstract:An acidic fraction from Ipomoea Nil showed suppression of SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in the Salmonella typhimurium TA1535/pSK1002 umu test. The suppressive compounds in I. Nil were fractionated by SiO 2 column chromatography. The active compounds in the suppressing fraction were identified as butanedioic acid, pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, nonanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid, hexadecanedioic acid, 3,4-dimethoxycinnamic acid, and linoleic acid by GC and GC-MS. These dibasic acids partially suppressed the SOS-inducing activity of AF-2. The dose-response values of all dibasic acids were in the range 0.3-1.5 μmol/mL. Tetradecanedioic acid suppressed 70% of the SOS-inducing activity of AF-2 under 1.5 μmol/mL. In addition tetradecanedioic acid was assayed with other mutagens (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]indole (Glu-P-1)), and it also showed a suppressive effect on each mutagen. The ID 50 (50% inhibitory dose) values of tetradecanedioic acid for AF-2, Trp-P-1, and Glu-P-1 were estimated as 0.44, 0.80, and 0.65 μmol/mL, respectively
-
Partial Suppression of SOS-Inducing Activity of Furylfuramide by Dibasic Acids from Ipomoea Nil in the Salmonella typhimurium TA1535/pSK1002 umu Test
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Mitsuo Miyazawa, Hideo Shimamura, Sei-ichi Nakamura, Hiromu KameokaAbstract:An acidic fraction from Ipomoea Nil showed suppression of SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in the Salmonella typhimurium TA1535/pSK1002 umu test. The suppressive compounds in I. Nil were fractionated by SiO 2 column chromatography. The active compounds in the suppressing fraction were identified as butanedioic acid, pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, nonanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid, hexadecanedioic acid, 3,4-dimethoxycinnamic acid, and linoleic acid by GC and GC-MS. These dibasic acids partially suppressed the SOS-inducing activity of AF-2. The dose-response values of all dibasic acids were in the range 0.3-1.5 μmol/mL. Tetradecanedioic acid suppressed 70% of the SOS-inducing activity of AF-2 under 1.5 μmol/mL. In addition tetradecanedioic acid was assayed with other mutagens (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]indole (Glu-P-1)), and it also showed a suppressive effect on each mutagen. The ID 50 (50% inhibitory dose) values of tetradecanedioic acid for AF-2, Trp-P-1, and Glu-P-1 were estimated as 0.44, 0.80, and 0.65 μmol/mL, respectively
Atsushi Hoshino - One of the best experts on this subject based on the ideXlab platform.
-
genome sequence and analysis of the japanese morning glory Ipomoea Nil
Nature Communications, 2016Co-Authors: Atsushi Hoshino, Eiji Nitasaka, Vasanthan Jayakumar, Atsushi Toyoda, Hideki Noguchi, Takehiko Itoh, Tadasu Shini, Yohei Minakuchi, Yuki KodaAbstract:Ipomoea is the largest genus in the family Convolvulaceae. Ipomoea Nil (Japanese morning glory) has been utilized as a model plant to study the genetic basis of floricultural traits, with over 1,500 mutant lines. In the present study, we have utilized second- and third-generation-sequencing platforms, and have reported a draft genome of I. Nil with a scaffold N50 of 2.88 Mb (contig N50 of 1.87 Mb), covering 98% of the 750 Mb genome. Scaffolds covering 91.42% of the assembly are anchored to 15 pseudo-chromosomes. The draft genome has enabled the identification and cataloguing of the Tpn1 family transposons, known as the major mutagen of I. Nil, and analysing the dwarf gene, CONTRACTED, located on the genetic map published in 1956. Comparative genomics has suggested that a whole genome duplication in Convolvulaceae, distinct from the recent Solanaceae event, has occurred after the divergence of the two sister families. Japanese morning glory (Ipomoea Nil) has diverse flowering traits. Here, the authors describe the reference genome sequence of I. Nil, annotations of genes and transposons, and compare evolution of the I. Nilgenome to other Convolvulaceae and Solanales genomes.
-
Development of EST-SSR markers of Ipomoea Nil
Breeding science, 2012Co-Authors: Hiroyuki Fukuoka, Shigeru Iida, Atsushi Hoshino, Asami Otaka, Eiji Nitasaka, Nobuyoshi Watanabe, Tsutomu KuboyamaAbstract:Although Japanese morning glory (Ipomoea Nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea Nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. Nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.
-
Identification of r mutations conferring white flowers in the Japanese morning glory (Ipomoea Nil)
Journal of Plant Research, 2009Co-Authors: Atsushi Hoshino, Kyeung-il Park, Shigeru IidaAbstract:The wild-type Japanese morning glory [ Ipomoea Nil (L.) Roth.] exhibits blue flowers with red stems, and spontaneous r mutants display white flowers with green stems. We have identified two r mutations, r1-1 and r1-2 , that are caused by insertions of Tpn1 -related DNA transposable elements, Tpn3 (5.6 kb) and Tpn6 (4.7 kb), respectively, into a unique intron of the CHS-D gene, which is responsible for flower and stem pigmentation. Both Tpn3 and Tpn6 , which belong to the En/Spm or CACTA superfamily, are nonautonomous elements lacking transposase genes but containing unrelated cellular DNA segments including exons and introns. Interestingly, r1-2 contains an additional 4-bp insertion at the Tpn3 integration site in r1-1 , presumably a footprint caused by the excision of Tpn3 . The results strengthen the previous notion that Tpn1 and its relatives are major spontaneous mutagens for generating various floriculturally important traits in I. Nil . Since I. Nil has an extensive history of genetic studies, molecular identification of classical spontaneous mutations would also facilitate reinterpretation of the abundant classical genetic data available.
-
Japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase, due to 4-bp insertio
The Plant journal : for cell and molecular biology, 2005Co-Authors: Yasumasa Morita, Norio Saito, Atsushi Hoshino, Eiji Nitasaka, Yasumasa Kikuchi, Hiroaki Okuhara, Eiichiro Ono, Yoshikazu Tanaka, Yuko Fukui, Hiroshi NoguchiAbstract:Bright blue or red flowers in the Japanese morning glory (Ipomoea Nil) contain anthocyanidin 3-O-sophoroside derivatives, whereas the reddish-brown or purplish-gray petals in its dusky mutants accumulate anthocyanidin 3-O-glucoside derivatives. The Dusky gene was found to encode a novel glucosyltransferase, UDP-glucose:anthocyanidin 3-O-glucoside-2''-O-glucosyltransferase (3GGT), which mediates the glucosylation of anthocyanidin 3-O-glucosides to yield anthocyanidin 3-O-sophorosides. Ipomoea Nil carries one copy of the 3GGT gene that contains no intron and produces 1.6-kbp transcripts mainly in the petals and tubes of flower buds at around 24 h before flower opening. The gene products of both In3GGT in I. Nil and Ip3GGT in the common morning glory (Ipomoea purpurea) comprise 459 amino acids and showed a close relationship to the petunia UDP-rhamnose:anthocyanidin 3-O-glucoside-6''-O-rhamnosyltransferase (3RT), which controls the addition of a rhamnose molecule to anthocyanidin 3-O-glucosides for conversion into anthocyanidin 3-O-rutinosides. All of the 30 dusky mutants tested were found to carry the 4-bp insertion mutations GGAT or CGAT at an identical position near the 3' end of the gene, and the insertions caused frameshift mutations. The expected 3GGT enzymatic activities were found in the crude extracts of Escherichia coli, in which the 3GGT cDNA of I. Nil or I. purpurea was expressed, while no such activity was detected in the extracts expressed with the dusky mutant cDNAs containing 4-bp insertions. Moreover, the introduced Ip3GGT cDNA efficiently produced 3GGT that converted cyanidin 3-O-glucoside into cyanidin 3-O-sophoroside in transgenic petunia plants.
-
Acylated peonidin glycosides from duskish mutant flowers of Ipomoea Nil.
Phytochemistry, 2005Co-Authors: Norio Saito, Yasumasa Morita, Kenjiro Toki, Atsushi Shigihara, Shigeru Iida, Atsushi Hoshino, Toshio HondaAbstract:Abstract Five acylated peonidin glycosides were isolated from the pale gray-purple flowers of a duskish mutant in the Japanese morning glory ( Ipomoea Nil or Pharbitis Nil ) as major pigments, along with a known anthocyanin, Heavenly Blue Anthocyanin (HBA). Three of these were based on peonidin 3-sophoroside and two on peonidin 3-sophoroside-5-glucoside as their deacylanthocyanins; both deacylanthocyanins were acylated with caffeic acid and/or glucosylcaffeic acids. By spectroscopic and chemical methods, the structures of the former three pigments were determined to be 3- O -[2- O -(6- O -( trans -caffeoyl)-β- D -glucopyranosyl)-β- D -glucopyranoside], 3- O -[2- O -(6- O -(3- O -(β- D -glucopyranosyl)- trans -caffeoyl)-β- D -glucopyranosyl)-6- O -(4- O -(6- O -(3- O -(β- D -glucopyranosyl)- trans -caffeoyl)-β- D -glucopyranosyl)- trans -caffeoyl)-β-glucopyranoside], and 3- O -[2- O -(6- O -( trans -caffeoyl)-β- D -glucopyranosyl)-6- O -(4- O -(6- O -(3- O -(β- D -glucopyranosyl)- trans -caffeoyl)-β- D -glucopyranosyl)- trans -caffeoyl)-β- D -glucopyranoside] of peonidin. The structures of the latter two pigments were also confirmed as 3- O -[2- O -(6- O -( trans -caffeoyl)-β- D -glucopyranosyl)-β- D -glucopyranoside]-5- O -β- D -glucopyranoside, and 3- O -[2- O -(6- O -( trans -caffeoyl)-β- D -glucopyranosyl)-6- O -(4- O -(6- O -(3- O -(β- D -glucopyranosyl)- trans -caffeoyl)-β- D -glucopyranosyl)- trans -caffeoyl)-β- D -glucopyranoside]-5- O -β- D -glucopyranoside of peonidin. The mutation affecting glycosylation and acylation in anthocyanin biosynthesis of Japanese morning glory was discussed.
Hideo Shimamura - One of the best experts on this subject based on the ideXlab platform.
-
partial suppression of sos inducing activity of furylfuramide by dibasic acids from Ipomoea Nil in the salmonella typhimurium ta1535 psk1002 umu test
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Mitsuo Miyazawa, Hideo Shimamura, Sei-ichi Nakamura, Hiromu KameokaAbstract:An acidic fraction from Ipomoea Nil showed suppression of SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in the Salmonella typhimurium TA1535/pSK1002 umu test. The suppressive compounds in I. Nil were fractionated by SiO 2 column chromatography. The active compounds in the suppressing fraction were identified as butanedioic acid, pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, nonanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid, hexadecanedioic acid, 3,4-dimethoxycinnamic acid, and linoleic acid by GC and GC-MS. These dibasic acids partially suppressed the SOS-inducing activity of AF-2. The dose-response values of all dibasic acids were in the range 0.3-1.5 μmol/mL. Tetradecanedioic acid suppressed 70% of the SOS-inducing activity of AF-2 under 1.5 μmol/mL. In addition tetradecanedioic acid was assayed with other mutagens (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]indole (Glu-P-1)), and it also showed a suppressive effect on each mutagen. The ID 50 (50% inhibitory dose) values of tetradecanedioic acid for AF-2, Trp-P-1, and Glu-P-1 were estimated as 0.44, 0.80, and 0.65 μmol/mL, respectively
-
Partial Suppression of SOS-Inducing Activity of Furylfuramide by Dibasic Acids from Ipomoea Nil in the Salmonella typhimurium TA1535/pSK1002 umu Test
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Mitsuo Miyazawa, Hideo Shimamura, Sei-ichi Nakamura, Hiromu KameokaAbstract:An acidic fraction from Ipomoea Nil showed suppression of SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in the Salmonella typhimurium TA1535/pSK1002 umu test. The suppressive compounds in I. Nil were fractionated by SiO 2 column chromatography. The active compounds in the suppressing fraction were identified as butanedioic acid, pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, nonanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid, hexadecanedioic acid, 3,4-dimethoxycinnamic acid, and linoleic acid by GC and GC-MS. These dibasic acids partially suppressed the SOS-inducing activity of AF-2. The dose-response values of all dibasic acids were in the range 0.3-1.5 μmol/mL. Tetradecanedioic acid suppressed 70% of the SOS-inducing activity of AF-2 under 1.5 μmol/mL. In addition tetradecanedioic acid was assayed with other mutagens (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]indole (Glu-P-1)), and it also showed a suppressive effect on each mutagen. The ID 50 (50% inhibitory dose) values of tetradecanedioic acid for AF-2, Trp-P-1, and Glu-P-1 were estimated as 0.44, 0.80, and 0.65 μmol/mL, respectively
Sei-ichi Nakamura - One of the best experts on this subject based on the ideXlab platform.
-
partial suppression of sos inducing activity of furylfuramide by dibasic acids from Ipomoea Nil in the salmonella typhimurium ta1535 psk1002 umu test
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Mitsuo Miyazawa, Hideo Shimamura, Sei-ichi Nakamura, Hiromu KameokaAbstract:An acidic fraction from Ipomoea Nil showed suppression of SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in the Salmonella typhimurium TA1535/pSK1002 umu test. The suppressive compounds in I. Nil were fractionated by SiO 2 column chromatography. The active compounds in the suppressing fraction were identified as butanedioic acid, pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, nonanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid, hexadecanedioic acid, 3,4-dimethoxycinnamic acid, and linoleic acid by GC and GC-MS. These dibasic acids partially suppressed the SOS-inducing activity of AF-2. The dose-response values of all dibasic acids were in the range 0.3-1.5 μmol/mL. Tetradecanedioic acid suppressed 70% of the SOS-inducing activity of AF-2 under 1.5 μmol/mL. In addition tetradecanedioic acid was assayed with other mutagens (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]indole (Glu-P-1)), and it also showed a suppressive effect on each mutagen. The ID 50 (50% inhibitory dose) values of tetradecanedioic acid for AF-2, Trp-P-1, and Glu-P-1 were estimated as 0.44, 0.80, and 0.65 μmol/mL, respectively
-
Partial Suppression of SOS-Inducing Activity of Furylfuramide by Dibasic Acids from Ipomoea Nil in the Salmonella typhimurium TA1535/pSK1002 umu Test
Journal of Agricultural and Food Chemistry, 1995Co-Authors: Mitsuo Miyazawa, Hideo Shimamura, Sei-ichi Nakamura, Hiromu KameokaAbstract:An acidic fraction from Ipomoea Nil showed suppression of SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) in the Salmonella typhimurium TA1535/pSK1002 umu test. The suppressive compounds in I. Nil were fractionated by SiO 2 column chromatography. The active compounds in the suppressing fraction were identified as butanedioic acid, pentanedioic acid, hexanedioic acid, heptanedioic acid, octanedioic acid, nonanedioic acid, decanedioic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid, hexadecanedioic acid, 3,4-dimethoxycinnamic acid, and linoleic acid by GC and GC-MS. These dibasic acids partially suppressed the SOS-inducing activity of AF-2. The dose-response values of all dibasic acids were in the range 0.3-1.5 μmol/mL. Tetradecanedioic acid suppressed 70% of the SOS-inducing activity of AF-2 under 1.5 μmol/mL. In addition tetradecanedioic acid was assayed with other mutagens (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]indole (Glu-P-1)), and it also showed a suppressive effect on each mutagen. The ID 50 (50% inhibitory dose) values of tetradecanedioic acid for AF-2, Trp-P-1, and Glu-P-1 were estimated as 0.44, 0.80, and 0.65 μmol/mL, respectively
