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Joseph E. Levinson - One of the best experts on this subject based on the ideXlab platform.
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antibodies to the 45 kda dek nuclear antigen in pauciarticular onset juvenile rheumatoid arthritis and Iridocyclitis selective association with mhc gene
The Journal of Rheumatology, 1997Co-Authors: Kevin J Murray, Edward H. Giannini, Joseph E. Levinson, David N. Glass, W Szer, Alexei A Grom, P Donnelly, I S SzerAbstract:Objective. To study the frequency of autoantibodies to the 45 kDa DEK nuclear antigen, a putative oncoprotein, in a sample of patients with juvenile rheumatoid arthritis (JRA), and to make correlations with disease subtype and complications such as Iridocyclitis. Class I and Class II HLA associations with reactivity to the antigen were also sought. Methods. Sera from 146 HLA typed patients with JRA representing all subtypes were analyzed for reactivity with the 45 kDa DEK protein by immunoblotting. The antigen was purified to near homogeneity from nuclei of HeLa cells. Results. Antibodies to DEK were found in 57% of all patients with JRA compared to 3% of controls (p < 0.0001). Antibodies were detected more frequently in pauciarticular onset (78%) than in polyarticular onset patients (29%; p < 0.01) and controls (3%; p < 0.0001). 97% of patients with JRA (regardless of onset subtype) and Iridocyclitis had anti-DEK antibodies compared to 47% of patients without eye disease (p < 0.0001). Anti-DEK antibodies were found more frequently in females compared to males in the pauciarticular onset disease group (84 vs 42%; p < 0.01). The occurrence of anti-DEK antibodies was closely associated with positive antinuclear antibody serology, and a strong association with the Class I gene HLA-A2 was also observed. Conclusion. Antibodies to the 45 kDa DEK protein are characteristic of the pauciarticular onset subtype of JRA, particularly in patients with a history of Iridocyclitis. The occurrence of anti-DEK antibodies is significantly but paradoxically associated with the presence of the HLA-A2 allele in such patients.
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Human leukocyte antigen-DRB1*1104 in the chronic Iridocyclitis of pauciarticular juvenile rheumatoid arthritis
The Journal of Pediatrics, 1992Co-Authors: Hector Melin-aldana, Edward H. Giannini, Janalee Taylor, Daniel J. Lovell, Joseph E. Levinson, Murray H. Passo, Joseph Ginsberg, Miles J. Burke, David N. GlassAbstract:To determine whether genetic markers for chronic Iridocyclitis could be identified, we used both serologic and oligonucleotide dot blot techniques to characterize immunogenetically 164 children with early-onset pauclarticular juvenile rheumatoid arthritis. Seventy-eight children (47.6%) had chronic Iridocyclitis and 86 (52.4%) had not had evidence of eye disease during a mean follow-up period after the onset of arthritis of 15.8 years (minimum of 5.5 years). Control subjects were 218 healthy, unrelated individuals. The analysis was limited to alleles known to be associated with an increased or decreased risk of early-onset pauciarticular juvenile rheumatoid arthritis or of chronic Iridocyclitis in this form of juvenile rheumatoid arthritis. Only one split of human leukocyte antigen (HLA)-DR5, HLA-DRB1 * 1104, showed a statistically significant association with a risk of chronic Iridocyclitis (chi-square value=7.52; p =0.036 adjusted; odds ratio 3.45); HLA-DQA1 * 0501 and HLA-DQB1 * 0301, both in linkage disequilibrium with HLA-DRB1 * 1104, also were significantly associated with eye disease. Patients with both the DRB1 * 1104 and DPB1 * 0201 genes had a 7.7-fold increased risk for chronic Iridocyclitis compared with that for other patients. The presence of HLA-DRB1 * 1104 was about four times as specific, but only about one third as sensitive, as antinuclear antibodies in identifying patients at risk for eye disease. Although all children with early-onset pauciarticular juvenile rheumatoid arthritis should undergo periodic slit-lamp examinations, those with the HLA class II gene DRB1 * 1104 are at particularly high risk for eye disease, and we recommend that they be monitored carefully for its evolution.
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human leukocyte antigen drb1 1104 in the chronic Iridocyclitis of pauciarticular juvenile rheumatoid arthritis
The Journal of Pediatrics, 1992Co-Authors: Edward H. Giannini, Janalee Taylor, Daniel J. Lovell, Joseph E. Levinson, Hector MelinaldanaAbstract:To determine whether genetic markers for chronic Iridocyclitis could be identified, we used both serologic and oligonucleotide dot blot techniques to characterize immunogenetically 164 children with early-onset pauclarticular juvenile rheumatoid arthritis. Seventy-eight children (47.6%) had chronic Iridocyclitis and 86 (52.4%) had not had evidence of eye disease during a mean follow-up period after the onset of arthritis of 15.8 years (minimum of 5.5 years). Control subjects were 218 healthy, unrelated individuals. The analysis was limited to alleles known to be associated with an increased or decreased risk of early-onset pauciarticular juvenile rheumatoid arthritis or of chronic Iridocyclitis in this form of juvenile rheumatoid arthritis. Only one split of human leukocyte antigen (HLA)-DR5, HLA-DRB1 * 1104, showed a statistically significant association with a risk of chronic Iridocyclitis (chi-square value=7.52; p =0.036 adjusted; odds ratio 3.45); HLA-DQA1 * 0501 and HLA-DQB1 * 0301, both in linkage disequilibrium with HLA-DRB1 * 1104, also were significantly associated with eye disease. Patients with both the DRB1 * 1104 and DPB1 * 0201 genes had a 7.7-fold increased risk for chronic Iridocyclitis compared with that for other patients. The presence of HLA-DRB1 * 1104 was about four times as specific, but only about one third as sensitive, as antinuclear antibodies in identifying patients at risk for eye disease. Although all children with early-onset pauciarticular juvenile rheumatoid arthritis should undergo periodic slit-lamp examinations, those with the HLA class II gene DRB1 * 1104 are at particularly high risk for eye disease, and we recommend that they be monitored carefully for its evolution.
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The Iridocyclitis of early onset pauciarticular juvenile rheumatoid arthritis: outcome in immunogenetically characterized patients.
The Journal of Rheumatology, 1992Co-Authors: C. N. Malagon, Edward H. Giannini, Janalee Taylor, Joseph E. Levinson, Murray H. Passo, C. Van Kerckhove, D J Lovell, J. Ginsberg, M. J. Burke, David N. GlassAbstract:In a cohort of 72 patients with Iridocyclitis (iritis) and early onset pauciarticular juvenile rheumatoid arthritis (EOPA-JRA) the course of the eye disease was matched with ocular outcome. Chronicity of inflammation (greater than 6 months/episode) was correlated with complications of eye disease that caused impairment of vision. HLA antigens in these patients were compared with the HLA antigens in a cohort of 77 patients with EOPA-JRA in whom Iridocyclitis had failed to develop over a followup of 5 years or longer. HLA-DR5 (11) was correlated with the presence of eye disease, and HLA-DR1 with its absence; HLA-DRw8, which strongly predisposes to EOPA-JRA, was neutral with respect to eye disease.
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Longitudinal analysis of HLA associated risks for Iridocyclitis in juvenile rheumatoid arthritis.
The Journal of Rheumatology, 1991Co-Authors: Edward H. Giannini, Janalee Taylor, Joseph E. Levinson, Murray H. Passo, C. N. Malagon, C. Van Kerckhove, D J Lovell, J. Ginsberg, M. J. Burke, David N. GlassAbstract:The risk of Iridocyclitis in children with early onset pauciarticular juvenile rheumatoid arthritis (EOPA-JRA) has been shown to be associated with certain HLA haplotypes. Our report contains an actuarial analysis, using one-year intervals, of 161 subjects and estimates haplotype specific risks. Individuals who possess the major susceptibility haplotype HLA-DR5 (11) developed eye disease earlier and with a greater frequency than did those with the protective HLA-DR1 haplotype. Highly significant differences were found between the resulting life-table curves for HLA-DR5 and HLA-DR1 positive subjects (p = 0.00003). These time oriented risk estimates may aid clinicians in determining more precisely the probability of Iridocyclitis throughout the course of the disease in children with EOPA-JRA.
Edward H. Giannini - One of the best experts on this subject based on the ideXlab platform.
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antibodies to the 45 kda dek nuclear antigen in pauciarticular onset juvenile rheumatoid arthritis and Iridocyclitis selective association with mhc gene
The Journal of Rheumatology, 1997Co-Authors: Kevin J Murray, Edward H. Giannini, Joseph E. Levinson, David N. Glass, W Szer, Alexei A Grom, P Donnelly, I S SzerAbstract:Objective. To study the frequency of autoantibodies to the 45 kDa DEK nuclear antigen, a putative oncoprotein, in a sample of patients with juvenile rheumatoid arthritis (JRA), and to make correlations with disease subtype and complications such as Iridocyclitis. Class I and Class II HLA associations with reactivity to the antigen were also sought. Methods. Sera from 146 HLA typed patients with JRA representing all subtypes were analyzed for reactivity with the 45 kDa DEK protein by immunoblotting. The antigen was purified to near homogeneity from nuclei of HeLa cells. Results. Antibodies to DEK were found in 57% of all patients with JRA compared to 3% of controls (p < 0.0001). Antibodies were detected more frequently in pauciarticular onset (78%) than in polyarticular onset patients (29%; p < 0.01) and controls (3%; p < 0.0001). 97% of patients with JRA (regardless of onset subtype) and Iridocyclitis had anti-DEK antibodies compared to 47% of patients without eye disease (p < 0.0001). Anti-DEK antibodies were found more frequently in females compared to males in the pauciarticular onset disease group (84 vs 42%; p < 0.01). The occurrence of anti-DEK antibodies was closely associated with positive antinuclear antibody serology, and a strong association with the Class I gene HLA-A2 was also observed. Conclusion. Antibodies to the 45 kDa DEK protein are characteristic of the pauciarticular onset subtype of JRA, particularly in patients with a history of Iridocyclitis. The occurrence of anti-DEK antibodies is significantly but paradoxically associated with the presence of the HLA-A2 allele in such patients.
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Human leukocyte antigen-DRB1*1104 in the chronic Iridocyclitis of pauciarticular juvenile rheumatoid arthritis
The Journal of Pediatrics, 1992Co-Authors: Hector Melin-aldana, Edward H. Giannini, Janalee Taylor, Daniel J. Lovell, Joseph E. Levinson, Murray H. Passo, Joseph Ginsberg, Miles J. Burke, David N. GlassAbstract:To determine whether genetic markers for chronic Iridocyclitis could be identified, we used both serologic and oligonucleotide dot blot techniques to characterize immunogenetically 164 children with early-onset pauclarticular juvenile rheumatoid arthritis. Seventy-eight children (47.6%) had chronic Iridocyclitis and 86 (52.4%) had not had evidence of eye disease during a mean follow-up period after the onset of arthritis of 15.8 years (minimum of 5.5 years). Control subjects were 218 healthy, unrelated individuals. The analysis was limited to alleles known to be associated with an increased or decreased risk of early-onset pauciarticular juvenile rheumatoid arthritis or of chronic Iridocyclitis in this form of juvenile rheumatoid arthritis. Only one split of human leukocyte antigen (HLA)-DR5, HLA-DRB1 * 1104, showed a statistically significant association with a risk of chronic Iridocyclitis (chi-square value=7.52; p =0.036 adjusted; odds ratio 3.45); HLA-DQA1 * 0501 and HLA-DQB1 * 0301, both in linkage disequilibrium with HLA-DRB1 * 1104, also were significantly associated with eye disease. Patients with both the DRB1 * 1104 and DPB1 * 0201 genes had a 7.7-fold increased risk for chronic Iridocyclitis compared with that for other patients. The presence of HLA-DRB1 * 1104 was about four times as specific, but only about one third as sensitive, as antinuclear antibodies in identifying patients at risk for eye disease. Although all children with early-onset pauciarticular juvenile rheumatoid arthritis should undergo periodic slit-lamp examinations, those with the HLA class II gene DRB1 * 1104 are at particularly high risk for eye disease, and we recommend that they be monitored carefully for its evolution.
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human leukocyte antigen drb1 1104 in the chronic Iridocyclitis of pauciarticular juvenile rheumatoid arthritis
The Journal of Pediatrics, 1992Co-Authors: Edward H. Giannini, Janalee Taylor, Daniel J. Lovell, Joseph E. Levinson, Hector MelinaldanaAbstract:To determine whether genetic markers for chronic Iridocyclitis could be identified, we used both serologic and oligonucleotide dot blot techniques to characterize immunogenetically 164 children with early-onset pauclarticular juvenile rheumatoid arthritis. Seventy-eight children (47.6%) had chronic Iridocyclitis and 86 (52.4%) had not had evidence of eye disease during a mean follow-up period after the onset of arthritis of 15.8 years (minimum of 5.5 years). Control subjects were 218 healthy, unrelated individuals. The analysis was limited to alleles known to be associated with an increased or decreased risk of early-onset pauciarticular juvenile rheumatoid arthritis or of chronic Iridocyclitis in this form of juvenile rheumatoid arthritis. Only one split of human leukocyte antigen (HLA)-DR5, HLA-DRB1 * 1104, showed a statistically significant association with a risk of chronic Iridocyclitis (chi-square value=7.52; p =0.036 adjusted; odds ratio 3.45); HLA-DQA1 * 0501 and HLA-DQB1 * 0301, both in linkage disequilibrium with HLA-DRB1 * 1104, also were significantly associated with eye disease. Patients with both the DRB1 * 1104 and DPB1 * 0201 genes had a 7.7-fold increased risk for chronic Iridocyclitis compared with that for other patients. The presence of HLA-DRB1 * 1104 was about four times as specific, but only about one third as sensitive, as antinuclear antibodies in identifying patients at risk for eye disease. Although all children with early-onset pauciarticular juvenile rheumatoid arthritis should undergo periodic slit-lamp examinations, those with the HLA class II gene DRB1 * 1104 are at particularly high risk for eye disease, and we recommend that they be monitored carefully for its evolution.
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The Iridocyclitis of early onset pauciarticular juvenile rheumatoid arthritis: outcome in immunogenetically characterized patients.
The Journal of Rheumatology, 1992Co-Authors: C. N. Malagon, Edward H. Giannini, Janalee Taylor, Joseph E. Levinson, Murray H. Passo, C. Van Kerckhove, D J Lovell, J. Ginsberg, M. J. Burke, David N. GlassAbstract:In a cohort of 72 patients with Iridocyclitis (iritis) and early onset pauciarticular juvenile rheumatoid arthritis (EOPA-JRA) the course of the eye disease was matched with ocular outcome. Chronicity of inflammation (greater than 6 months/episode) was correlated with complications of eye disease that caused impairment of vision. HLA antigens in these patients were compared with the HLA antigens in a cohort of 77 patients with EOPA-JRA in whom Iridocyclitis had failed to develop over a followup of 5 years or longer. HLA-DR5 (11) was correlated with the presence of eye disease, and HLA-DR1 with its absence; HLA-DRw8, which strongly predisposes to EOPA-JRA, was neutral with respect to eye disease.
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Longitudinal analysis of HLA associated risks for Iridocyclitis in juvenile rheumatoid arthritis.
The Journal of Rheumatology, 1991Co-Authors: Edward H. Giannini, Janalee Taylor, Joseph E. Levinson, Murray H. Passo, C. N. Malagon, C. Van Kerckhove, D J Lovell, J. Ginsberg, M. J. Burke, David N. GlassAbstract:The risk of Iridocyclitis in children with early onset pauciarticular juvenile rheumatoid arthritis (EOPA-JRA) has been shown to be associated with certain HLA haplotypes. Our report contains an actuarial analysis, using one-year intervals, of 161 subjects and estimates haplotype specific risks. Individuals who possess the major susceptibility haplotype HLA-DR5 (11) developed eye disease earlier and with a greater frequency than did those with the protective HLA-DR1 haplotype. Highly significant differences were found between the resulting life-table curves for HLA-DR5 and HLA-DR1 positive subjects (p = 0.00003). These time oriented risk estimates may aid clinicians in determining more precisely the probability of Iridocyclitis throughout the course of the disease in children with EOPA-JRA.
Yasuo Tano - One of the best experts on this subject based on the ideXlab platform.
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detecting varicella zoster virus dna in Iridocyclitis using polymerase chain reaction a case of zoster sine herpete
Archives of Ophthalmology, 1995Co-Authors: Shuji Yamamoto, Rei Tada, Yoshikazu Shimomura, Deborah Pavanlangston, Edmund C Dunkel, Yasuo TanoAbstract:Varicella-zoster virus (VZV) is a recognized etiologic agent in Iridocyclitis. 1 However, diagnosing zoster Iridocyclitis without the presence of dermatitis (zoster sine herpete 2 ) is difficult. We describe a patient with Iridocyclitis in whom VZV DNA was detected using the polymerase chain reaction (PCR). To our knowledge, this is the first such case of PCR detection of VZV DNA in zoster keratouveitis. Report of a Case. A healthy 72-year-old woman, referred to us with a diagnosis of anterior uveitis in the right eye, complained of redness and photophobia in the right eye. Best corrected visual acuities were 20/40 OD and 20/20 OS. Intraocular pressures were 2 mm Hg in the right eye and 12 mm Hg in the left. Slit-lamp examination showed severe Descemet's folds with pigmented keratic precipitates in the right eye ( Figure 1 , left) and mild cells and flare in the anterior chamber. The corneal epithelium was intact.
Janalee Taylor - One of the best experts on this subject based on the ideXlab platform.
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Human leukocyte antigen-DRB1*1104 in the chronic Iridocyclitis of pauciarticular juvenile rheumatoid arthritis
The Journal of Pediatrics, 1992Co-Authors: Hector Melin-aldana, Edward H. Giannini, Janalee Taylor, Daniel J. Lovell, Joseph E. Levinson, Murray H. Passo, Joseph Ginsberg, Miles J. Burke, David N. GlassAbstract:To determine whether genetic markers for chronic Iridocyclitis could be identified, we used both serologic and oligonucleotide dot blot techniques to characterize immunogenetically 164 children with early-onset pauclarticular juvenile rheumatoid arthritis. Seventy-eight children (47.6%) had chronic Iridocyclitis and 86 (52.4%) had not had evidence of eye disease during a mean follow-up period after the onset of arthritis of 15.8 years (minimum of 5.5 years). Control subjects were 218 healthy, unrelated individuals. The analysis was limited to alleles known to be associated with an increased or decreased risk of early-onset pauciarticular juvenile rheumatoid arthritis or of chronic Iridocyclitis in this form of juvenile rheumatoid arthritis. Only one split of human leukocyte antigen (HLA)-DR5, HLA-DRB1 * 1104, showed a statistically significant association with a risk of chronic Iridocyclitis (chi-square value=7.52; p =0.036 adjusted; odds ratio 3.45); HLA-DQA1 * 0501 and HLA-DQB1 * 0301, both in linkage disequilibrium with HLA-DRB1 * 1104, also were significantly associated with eye disease. Patients with both the DRB1 * 1104 and DPB1 * 0201 genes had a 7.7-fold increased risk for chronic Iridocyclitis compared with that for other patients. The presence of HLA-DRB1 * 1104 was about four times as specific, but only about one third as sensitive, as antinuclear antibodies in identifying patients at risk for eye disease. Although all children with early-onset pauciarticular juvenile rheumatoid arthritis should undergo periodic slit-lamp examinations, those with the HLA class II gene DRB1 * 1104 are at particularly high risk for eye disease, and we recommend that they be monitored carefully for its evolution.
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human leukocyte antigen drb1 1104 in the chronic Iridocyclitis of pauciarticular juvenile rheumatoid arthritis
The Journal of Pediatrics, 1992Co-Authors: Edward H. Giannini, Janalee Taylor, Daniel J. Lovell, Joseph E. Levinson, Hector MelinaldanaAbstract:To determine whether genetic markers for chronic Iridocyclitis could be identified, we used both serologic and oligonucleotide dot blot techniques to characterize immunogenetically 164 children with early-onset pauclarticular juvenile rheumatoid arthritis. Seventy-eight children (47.6%) had chronic Iridocyclitis and 86 (52.4%) had not had evidence of eye disease during a mean follow-up period after the onset of arthritis of 15.8 years (minimum of 5.5 years). Control subjects were 218 healthy, unrelated individuals. The analysis was limited to alleles known to be associated with an increased or decreased risk of early-onset pauciarticular juvenile rheumatoid arthritis or of chronic Iridocyclitis in this form of juvenile rheumatoid arthritis. Only one split of human leukocyte antigen (HLA)-DR5, HLA-DRB1 * 1104, showed a statistically significant association with a risk of chronic Iridocyclitis (chi-square value=7.52; p =0.036 adjusted; odds ratio 3.45); HLA-DQA1 * 0501 and HLA-DQB1 * 0301, both in linkage disequilibrium with HLA-DRB1 * 1104, also were significantly associated with eye disease. Patients with both the DRB1 * 1104 and DPB1 * 0201 genes had a 7.7-fold increased risk for chronic Iridocyclitis compared with that for other patients. The presence of HLA-DRB1 * 1104 was about four times as specific, but only about one third as sensitive, as antinuclear antibodies in identifying patients at risk for eye disease. Although all children with early-onset pauciarticular juvenile rheumatoid arthritis should undergo periodic slit-lamp examinations, those with the HLA class II gene DRB1 * 1104 are at particularly high risk for eye disease, and we recommend that they be monitored carefully for its evolution.
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The Iridocyclitis of early onset pauciarticular juvenile rheumatoid arthritis: outcome in immunogenetically characterized patients.
The Journal of Rheumatology, 1992Co-Authors: C. N. Malagon, Edward H. Giannini, Janalee Taylor, Joseph E. Levinson, Murray H. Passo, C. Van Kerckhove, D J Lovell, J. Ginsberg, M. J. Burke, David N. GlassAbstract:In a cohort of 72 patients with Iridocyclitis (iritis) and early onset pauciarticular juvenile rheumatoid arthritis (EOPA-JRA) the course of the eye disease was matched with ocular outcome. Chronicity of inflammation (greater than 6 months/episode) was correlated with complications of eye disease that caused impairment of vision. HLA antigens in these patients were compared with the HLA antigens in a cohort of 77 patients with EOPA-JRA in whom Iridocyclitis had failed to develop over a followup of 5 years or longer. HLA-DR5 (11) was correlated with the presence of eye disease, and HLA-DR1 with its absence; HLA-DRw8, which strongly predisposes to EOPA-JRA, was neutral with respect to eye disease.
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Longitudinal analysis of HLA associated risks for Iridocyclitis in juvenile rheumatoid arthritis.
The Journal of Rheumatology, 1991Co-Authors: Edward H. Giannini, Janalee Taylor, Joseph E. Levinson, Murray H. Passo, C. N. Malagon, C. Van Kerckhove, D J Lovell, J. Ginsberg, M. J. Burke, David N. GlassAbstract:The risk of Iridocyclitis in children with early onset pauciarticular juvenile rheumatoid arthritis (EOPA-JRA) has been shown to be associated with certain HLA haplotypes. Our report contains an actuarial analysis, using one-year intervals, of 161 subjects and estimates haplotype specific risks. Individuals who possess the major susceptibility haplotype HLA-DR5 (11) developed eye disease earlier and with a greater frequency than did those with the protective HLA-DR1 haplotype. Highly significant differences were found between the resulting life-table curves for HLA-DR5 and HLA-DR1 positive subjects (p = 0.00003). These time oriented risk estimates may aid clinicians in determining more precisely the probability of Iridocyclitis throughout the course of the disease in children with EOPA-JRA.
David N. Glass - One of the best experts on this subject based on the ideXlab platform.
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antibodies to the 45 kda dek nuclear antigen in pauciarticular onset juvenile rheumatoid arthritis and Iridocyclitis selective association with mhc gene
The Journal of Rheumatology, 1997Co-Authors: Kevin J Murray, Edward H. Giannini, Joseph E. Levinson, David N. Glass, W Szer, Alexei A Grom, P Donnelly, I S SzerAbstract:Objective. To study the frequency of autoantibodies to the 45 kDa DEK nuclear antigen, a putative oncoprotein, in a sample of patients with juvenile rheumatoid arthritis (JRA), and to make correlations with disease subtype and complications such as Iridocyclitis. Class I and Class II HLA associations with reactivity to the antigen were also sought. Methods. Sera from 146 HLA typed patients with JRA representing all subtypes were analyzed for reactivity with the 45 kDa DEK protein by immunoblotting. The antigen was purified to near homogeneity from nuclei of HeLa cells. Results. Antibodies to DEK were found in 57% of all patients with JRA compared to 3% of controls (p < 0.0001). Antibodies were detected more frequently in pauciarticular onset (78%) than in polyarticular onset patients (29%; p < 0.01) and controls (3%; p < 0.0001). 97% of patients with JRA (regardless of onset subtype) and Iridocyclitis had anti-DEK antibodies compared to 47% of patients without eye disease (p < 0.0001). Anti-DEK antibodies were found more frequently in females compared to males in the pauciarticular onset disease group (84 vs 42%; p < 0.01). The occurrence of anti-DEK antibodies was closely associated with positive antinuclear antibody serology, and a strong association with the Class I gene HLA-A2 was also observed. Conclusion. Antibodies to the 45 kDa DEK protein are characteristic of the pauciarticular onset subtype of JRA, particularly in patients with a history of Iridocyclitis. The occurrence of anti-DEK antibodies is significantly but paradoxically associated with the presence of the HLA-A2 allele in such patients.
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Human leukocyte antigen-DRB1*1104 in the chronic Iridocyclitis of pauciarticular juvenile rheumatoid arthritis
The Journal of Pediatrics, 1992Co-Authors: Hector Melin-aldana, Edward H. Giannini, Janalee Taylor, Daniel J. Lovell, Joseph E. Levinson, Murray H. Passo, Joseph Ginsberg, Miles J. Burke, David N. GlassAbstract:To determine whether genetic markers for chronic Iridocyclitis could be identified, we used both serologic and oligonucleotide dot blot techniques to characterize immunogenetically 164 children with early-onset pauclarticular juvenile rheumatoid arthritis. Seventy-eight children (47.6%) had chronic Iridocyclitis and 86 (52.4%) had not had evidence of eye disease during a mean follow-up period after the onset of arthritis of 15.8 years (minimum of 5.5 years). Control subjects were 218 healthy, unrelated individuals. The analysis was limited to alleles known to be associated with an increased or decreased risk of early-onset pauciarticular juvenile rheumatoid arthritis or of chronic Iridocyclitis in this form of juvenile rheumatoid arthritis. Only one split of human leukocyte antigen (HLA)-DR5, HLA-DRB1 * 1104, showed a statistically significant association with a risk of chronic Iridocyclitis (chi-square value=7.52; p =0.036 adjusted; odds ratio 3.45); HLA-DQA1 * 0501 and HLA-DQB1 * 0301, both in linkage disequilibrium with HLA-DRB1 * 1104, also were significantly associated with eye disease. Patients with both the DRB1 * 1104 and DPB1 * 0201 genes had a 7.7-fold increased risk for chronic Iridocyclitis compared with that for other patients. The presence of HLA-DRB1 * 1104 was about four times as specific, but only about one third as sensitive, as antinuclear antibodies in identifying patients at risk for eye disease. Although all children with early-onset pauciarticular juvenile rheumatoid arthritis should undergo periodic slit-lamp examinations, those with the HLA class II gene DRB1 * 1104 are at particularly high risk for eye disease, and we recommend that they be monitored carefully for its evolution.
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The Iridocyclitis of early onset pauciarticular juvenile rheumatoid arthritis: outcome in immunogenetically characterized patients.
The Journal of Rheumatology, 1992Co-Authors: C. N. Malagon, Edward H. Giannini, Janalee Taylor, Joseph E. Levinson, Murray H. Passo, C. Van Kerckhove, D J Lovell, J. Ginsberg, M. J. Burke, David N. GlassAbstract:In a cohort of 72 patients with Iridocyclitis (iritis) and early onset pauciarticular juvenile rheumatoid arthritis (EOPA-JRA) the course of the eye disease was matched with ocular outcome. Chronicity of inflammation (greater than 6 months/episode) was correlated with complications of eye disease that caused impairment of vision. HLA antigens in these patients were compared with the HLA antigens in a cohort of 77 patients with EOPA-JRA in whom Iridocyclitis had failed to develop over a followup of 5 years or longer. HLA-DR5 (11) was correlated with the presence of eye disease, and HLA-DR1 with its absence; HLA-DRw8, which strongly predisposes to EOPA-JRA, was neutral with respect to eye disease.
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Longitudinal analysis of HLA associated risks for Iridocyclitis in juvenile rheumatoid arthritis.
The Journal of Rheumatology, 1991Co-Authors: Edward H. Giannini, Janalee Taylor, Joseph E. Levinson, Murray H. Passo, C. N. Malagon, C. Van Kerckhove, D J Lovell, J. Ginsberg, M. J. Burke, David N. GlassAbstract:The risk of Iridocyclitis in children with early onset pauciarticular juvenile rheumatoid arthritis (EOPA-JRA) has been shown to be associated with certain HLA haplotypes. Our report contains an actuarial analysis, using one-year intervals, of 161 subjects and estimates haplotype specific risks. Individuals who possess the major susceptibility haplotype HLA-DR5 (11) developed eye disease earlier and with a greater frequency than did those with the protective HLA-DR1 haplotype. Highly significant differences were found between the resulting life-table curves for HLA-DR5 and HLA-DR1 positive subjects (p = 0.00003). These time oriented risk estimates may aid clinicians in determining more precisely the probability of Iridocyclitis throughout the course of the disease in children with EOPA-JRA.
