Iron Binding Capacity

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Yoshinori Iwatani - One of the best experts on this subject based on the ideXlab platform.

  • Modification of the Colorimetric Assay for Serum Unsaturated Iron-Binding Capacity
    Clinical Chemistry, 2003
    Co-Authors: Hachiro Yamanishi, Shigeru Iyama, Yoshihisa Yamaguchi, Yuzuru Kanakura, Yoshinori Iwatani
    Abstract:

    We reported recently that total Iron-Binding Capacity (TIBC) values calculated from serum Iron and unsaturated Iron-Binding Capacity (UIBC) values were significantly lower than those obtained by a direct and fully automated TIBC assay (1)(2). We also reported that slopes of regression lines for calculated TIBC values plotted against serum transferrin (TRF) were ∼7% lower than the theoretical ratio of TIBC to TRF (TIBC/TRF = 25.1 μmol/g). We found that this could be attributed to underestimation of UIBC values by colorimetric methods. One possible reason for underestimation of UIBC values was insufficient saturation of TRF. We modified the assay conditions of a colorimetric method for UIBC measurement to improve the correspondence between TIBC values converted from TRF and those calculated from serum Iron and UIBC. Both serum Iron and UIBC were determined by colorimetric methods (Wako Pure Chemical Industries) with a Hitachi Model 7070 automated analyzer. Serum TRF concentrations were determined by a nephelometric assay on a Behring Nephelometer II analyzer (Dade Behring). UIBC values were determined by four modified methods (Table 1⇓ ). UIBCA was …

  • Total Iron-Binding Capacity calculated from serum transferrin concentration or serum Iron concentration and unsaturated Iron-Binding Capacity.
    Clinical chemistry, 2003
    Co-Authors: Hachiro Yamanishi, Shigeru Iyama, Yoshihisa Yamaguchi, Yuzuru Kanakura, Yoshinori Iwatani
    Abstract:

    Total Iron-Binding Capacity (TIBC) indicates the maximum amount of Iron needed to saturate plasma or serum transferrin (TRF), which is the primary Iron-transport protein (1). Theoretically, 1 mol of TRF [average molecular mass, 79 570 Da (2)] can bind 2 mol of Iron (55.8 Da) at two high-affinity Binding sites for ferric Iron (3). Therefore, TIBC correlates well with TRF concentration, and the theoretical ratio of TIBC (in μmol/L) to TRF (in g/L) is 25.1: TIBC (μmol/L) = 25.1 × TRF (g/L) (4)(5). Measurements of TIBC, serum Iron, and the percentage of Iron saturation of TRF are useful for the clinical diagnosis of Iron-deficiency anemia and chronic inflammatory disorders (6)(7) and as screening tests for other clinical conditions (8). TIBC is routinely determined (9)(10)(11)(12) by saturation of TRF with an excess predetermined amount of Iron, removal of the unbound Iron, and measurement of the Iron that is dissociated from TRF. For removal of the unbound Iron, magnesium carbonate (9), ion-exchange resin (10), alumina columns (11), or magnetic particles(12) are used. Most direct TIBC measurement methods require manual procedures that involve centrifugation or pretreatment of serum samples. As an alternative to direct measurement methods, TIBC values are also calculated from the sum of serum Iron and unsaturated Iron-Binding Capacity (UIBC), both of which are determined by colorimetric methods (calculation method). We developed a direct and fully automated TIBC (DTIBC) assay for use with an automated multipurpose analyzer (13)(14). A fully automated TIBC measurement method is also commercially available (15). In our previous study (14), TIBC values obtained by DTIBC assay correlated strongly with serum TRF concentrations ( r = 0.984; n = 59), and the slope of the regression line was consistent with the theoretical TIBC/TRF ratio. We …

Hachiro Yamanishi - One of the best experts on this subject based on the ideXlab platform.

  • Modification of the Colorimetric Assay for Serum Unsaturated Iron-Binding Capacity
    Clinical Chemistry, 2003
    Co-Authors: Hachiro Yamanishi, Shigeru Iyama, Yoshihisa Yamaguchi, Yuzuru Kanakura, Yoshinori Iwatani
    Abstract:

    We reported recently that total Iron-Binding Capacity (TIBC) values calculated from serum Iron and unsaturated Iron-Binding Capacity (UIBC) values were significantly lower than those obtained by a direct and fully automated TIBC assay (1)(2). We also reported that slopes of regression lines for calculated TIBC values plotted against serum transferrin (TRF) were ∼7% lower than the theoretical ratio of TIBC to TRF (TIBC/TRF = 25.1 μmol/g). We found that this could be attributed to underestimation of UIBC values by colorimetric methods. One possible reason for underestimation of UIBC values was insufficient saturation of TRF. We modified the assay conditions of a colorimetric method for UIBC measurement to improve the correspondence between TIBC values converted from TRF and those calculated from serum Iron and UIBC. Both serum Iron and UIBC were determined by colorimetric methods (Wako Pure Chemical Industries) with a Hitachi Model 7070 automated analyzer. Serum TRF concentrations were determined by a nephelometric assay on a Behring Nephelometer II analyzer (Dade Behring). UIBC values were determined by four modified methods (Table 1⇓ ). UIBCA was …

  • Total Iron-Binding Capacity calculated from serum transferrin concentration or serum Iron concentration and unsaturated Iron-Binding Capacity.
    Clinical chemistry, 2003
    Co-Authors: Hachiro Yamanishi, Shigeru Iyama, Yoshihisa Yamaguchi, Yuzuru Kanakura, Yoshinori Iwatani
    Abstract:

    Total Iron-Binding Capacity (TIBC) indicates the maximum amount of Iron needed to saturate plasma or serum transferrin (TRF), which is the primary Iron-transport protein (1). Theoretically, 1 mol of TRF [average molecular mass, 79 570 Da (2)] can bind 2 mol of Iron (55.8 Da) at two high-affinity Binding sites for ferric Iron (3). Therefore, TIBC correlates well with TRF concentration, and the theoretical ratio of TIBC (in μmol/L) to TRF (in g/L) is 25.1: TIBC (μmol/L) = 25.1 × TRF (g/L) (4)(5). Measurements of TIBC, serum Iron, and the percentage of Iron saturation of TRF are useful for the clinical diagnosis of Iron-deficiency anemia and chronic inflammatory disorders (6)(7) and as screening tests for other clinical conditions (8). TIBC is routinely determined (9)(10)(11)(12) by saturation of TRF with an excess predetermined amount of Iron, removal of the unbound Iron, and measurement of the Iron that is dissociated from TRF. For removal of the unbound Iron, magnesium carbonate (9), ion-exchange resin (10), alumina columns (11), or magnetic particles(12) are used. Most direct TIBC measurement methods require manual procedures that involve centrifugation or pretreatment of serum samples. As an alternative to direct measurement methods, TIBC values are also calculated from the sum of serum Iron and unsaturated Iron-Binding Capacity (UIBC), both of which are determined by colorimetric methods (calculation method). We developed a direct and fully automated TIBC (DTIBC) assay for use with an automated multipurpose analyzer (13)(14). A fully automated TIBC measurement method is also commercially available (15). In our previous study (14), TIBC values obtained by DTIBC assay correlated strongly with serum TRF concentrations ( r = 0.984; n = 59), and the slope of the regression line was consistent with the theoretical TIBC/TRF ratio. We …

O. F. Idris - One of the best experts on this subject based on the ideXlab platform.

  • Serum Iron and serum IronBinding Capacity in the Dromedary (Camelus dromedarius)
    Journal of Zoology, 2010
    Co-Authors: G. Tartour, O. F. Idris
    Abstract:

    Serum Iron and the Binding Capacity of serum were investigated in 66 pack and 70 riding camels in the Sudan. The total Iron-Binding Capacity was comparable with means and ranges recorded for other domestic animals but the serum Iron and the saturation concentration were both lower. Intersex differences were observed only in the total Iron-Binding Capacity and the saturation concentration. Both serum Iron and total Iron-Binding Capacity were greater in the riding than in the pack animals. The results also suggested absence of relationship between the process of ageing in the camel and the various parameters.

  • serum Iron and serum Iron Binding Capacity in the dromedary camelus dromedarius
    Journal of Zoology, 2010
    Co-Authors: G. Tartour, O. F. Idris
    Abstract:

    Serum Iron and the Binding Capacity of serum were investigated in 66 pack and 70 riding camels in the Sudan. The total Iron-Binding Capacity was comparable with means and ranges recorded for other domestic animals but the serum Iron and the saturation concentration were both lower. Intersex differences were observed only in the total Iron-Binding Capacity and the saturation concentration. Both serum Iron and total Iron-Binding Capacity were greater in the riding than in the pack animals. The results also suggested absence of relationship between the process of ageing in the camel and the various parameters.

Paul C Adams - One of the best experts on this subject based on the ideXlab platform.

  • biological variability of transferrin saturation and unsaturated Iron Binding Capacity
    The American Journal of Medicine, 2007
    Co-Authors: Paul C Adams, David M Reboussin, Richard D Press, James C Barton, Ronald T Acton, Godfrey C Moses, Catherine Leiendeckerfoster, Gordon D Mclaren, Fitzroy W Dawkins, Victor R Gordeuk
    Abstract:

    Abstract Background Transferrin saturation is widely considered the preferred screening test for hemochromatosis. Unsaturated Iron-Binding Capacity has similar performance at lower cost. However, the within-person biological variability of both these tests may limit their ability at commonly used cut points to detect HFE C282Y homozygous patients. Methods The Hemochromatosis and Iron Overload Screening Study screened 101,168 primary care participants for Iron overload using transferrin saturation, unsaturated Iron-Binding Capacity, ferritin, and HFE C282Y and H63D genotyping. Transferrin saturation and unsaturated Iron-Binding Capacity were performed at initial screening and again when selected participants and controls returned for a clinical examination several months later. A missed case was defined as a C282Y homozygote who had transferrin saturation below the cut point (45% for women, 50% for men) or unsaturated Iron-Binding Capacity above the cut point (150 μmol/L for women, 125 μmol/L for men) at the initial screening or the clinical examination, or both, regardless of serum ferritin. Results There were 209 C282Y previously undiagnosed homozygotes with transferrin saturation and unsaturated Iron-Binding Capacity testing performed at the initial screening and clinical examination. Sixty-eight C282Y homozygotes (33%) would have been missed at these transferrin saturation cut points (19 men, 49 women; median serum ferritin level of 170 μg/L; first and third quartiles, 50 and 474 μg/L), and 58 homozygotes (28%) would have been missed at the unsaturated Iron-Binding Capacity cut points (20 men, 38 women; median serum ferritin level of 168 μg/L; first and third quartiles, 38 and 454 μg/L). There was no advantage to using fasting samples. Conclusions The within-person biological variability of transferrin saturation and unsaturated Iron-Binding Capacity limits their usefulness as an initial screening test for expressing C282Y homozygotes.

  • Unsaturated Iron-Binding Capacity: a screening test for C282Y hemochromatosis?
    Clinical chemistry, 2000
    Co-Authors: Paul C Adams, Vipin Bhayana
    Abstract:

    Transferrin saturation (TS) has been recommended for screening for hemochromatosis (1). It is widely available, and results may be increased even in young adults with hemochromatosis. The TS assay is a two-step assay with serum Iron in the numerator and total Iron-Binding Capacity, unsaturated Iron-Binding Capacity (UIBC), or serum transferrin in the denominator. Serum Iron/(serum Iron + UIBC) equals the TS. In a previous study, we compared UIBC to TS as a screening test for C282Y hemochromatosis in a population of asymptomatic voluntary blood donors (2). Because blood donation could potentially affect Iron status, we have reevaluated TS and UIBC in referred hemochromatosis patients and used first-time blood donors as control cases (n = 386, all …

Yoshihisa Yamaguchi - One of the best experts on this subject based on the ideXlab platform.

  • Modification of the Colorimetric Assay for Serum Unsaturated Iron-Binding Capacity
    Clinical Chemistry, 2003
    Co-Authors: Hachiro Yamanishi, Shigeru Iyama, Yoshihisa Yamaguchi, Yuzuru Kanakura, Yoshinori Iwatani
    Abstract:

    We reported recently that total Iron-Binding Capacity (TIBC) values calculated from serum Iron and unsaturated Iron-Binding Capacity (UIBC) values were significantly lower than those obtained by a direct and fully automated TIBC assay (1)(2). We also reported that slopes of regression lines for calculated TIBC values plotted against serum transferrin (TRF) were ∼7% lower than the theoretical ratio of TIBC to TRF (TIBC/TRF = 25.1 μmol/g). We found that this could be attributed to underestimation of UIBC values by colorimetric methods. One possible reason for underestimation of UIBC values was insufficient saturation of TRF. We modified the assay conditions of a colorimetric method for UIBC measurement to improve the correspondence between TIBC values converted from TRF and those calculated from serum Iron and UIBC. Both serum Iron and UIBC were determined by colorimetric methods (Wako Pure Chemical Industries) with a Hitachi Model 7070 automated analyzer. Serum TRF concentrations were determined by a nephelometric assay on a Behring Nephelometer II analyzer (Dade Behring). UIBC values were determined by four modified methods (Table 1⇓ ). UIBCA was …

  • Total Iron-Binding Capacity calculated from serum transferrin concentration or serum Iron concentration and unsaturated Iron-Binding Capacity.
    Clinical chemistry, 2003
    Co-Authors: Hachiro Yamanishi, Shigeru Iyama, Yoshihisa Yamaguchi, Yuzuru Kanakura, Yoshinori Iwatani
    Abstract:

    Total Iron-Binding Capacity (TIBC) indicates the maximum amount of Iron needed to saturate plasma or serum transferrin (TRF), which is the primary Iron-transport protein (1). Theoretically, 1 mol of TRF [average molecular mass, 79 570 Da (2)] can bind 2 mol of Iron (55.8 Da) at two high-affinity Binding sites for ferric Iron (3). Therefore, TIBC correlates well with TRF concentration, and the theoretical ratio of TIBC (in μmol/L) to TRF (in g/L) is 25.1: TIBC (μmol/L) = 25.1 × TRF (g/L) (4)(5). Measurements of TIBC, serum Iron, and the percentage of Iron saturation of TRF are useful for the clinical diagnosis of Iron-deficiency anemia and chronic inflammatory disorders (6)(7) and as screening tests for other clinical conditions (8). TIBC is routinely determined (9)(10)(11)(12) by saturation of TRF with an excess predetermined amount of Iron, removal of the unbound Iron, and measurement of the Iron that is dissociated from TRF. For removal of the unbound Iron, magnesium carbonate (9), ion-exchange resin (10), alumina columns (11), or magnetic particles(12) are used. Most direct TIBC measurement methods require manual procedures that involve centrifugation or pretreatment of serum samples. As an alternative to direct measurement methods, TIBC values are also calculated from the sum of serum Iron and unsaturated Iron-Binding Capacity (UIBC), both of which are determined by colorimetric methods (calculation method). We developed a direct and fully automated TIBC (DTIBC) assay for use with an automated multipurpose analyzer (13)(14). A fully automated TIBC measurement method is also commercially available (15). In our previous study (14), TIBC values obtained by DTIBC assay correlated strongly with serum TRF concentrations ( r = 0.984; n = 59), and the slope of the regression line was consistent with the theoretical TIBC/TRF ratio. We …