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Ewa Kulczykowska - One of the best experts on this subject based on the ideXlab platform.
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nonapeptides arginine vasotocin and Isotocin in fishes advantage of bioactive molecules measurement
Frontiers in Marine Science, 2020Co-Authors: Ewa Sokolowska, Magdalena Gozdowska, Ewa KulczykowskaAbstract:The aim of this article is to share with fish physiologists and behaviourists our thoughts and hints regarding the involvement of nonapeptides in various physiological and behavioural processes in fishes and the advantage of measurements of bioavailable molecules. Arginine vasotocin (AVT) and Isotocin (IT) are biologically active nonapeptides in teleosts which are produced in separate neurosecretory neurons in the hypothalamic nuclei. They act as neurotransmitters and/or neuromodulators in the central nervous system (CNS) or as peripheral hormones, when they are released in the neurohypophysis and distributed via the bloodstream. Biosynthesis of bioactive AVT and IT is a complicated multistage process and nonapeptides have multiple sites of action. Different analytical approaches, including determination of pro-AVT and pro-IT mRNA in the hypothalamus or AVT and IT concentration in the brain, pituitary and plasma are used depending on the aim of the study. However, the level of biologically-active molecules is more straightforwardly related to their current action in the CNS and/or peripheral organs compared with indirect proxies of nonapeptides production such as the expression of pro-peptides mRNA. Therefore analysis of AVT and IT is highly recommended in many studies. Herein we compiled various works in which bioavailable AVT and IT were analysed in order to highlight the importance of such approach. Moreover, we compared the fluctuation of nonapeptide levels in the plasma with that in the brain of round gobies in successive reproductive phases. We also showed that neither AVT nor IT level was associated with fish age.
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Whole brain nonapeptide’ levels in clients (Naso elegans).
2017Co-Authors: Marta C. Soares, Sónia C. Cardoso, Renata Mazzei, Gonçalo I. André, Marta Morais, Magdalena Gozdowska, Hanna Kalamarz-kubiak, Ewa KulczykowskaAbstract:(A) levels of arginine vasotocin (AVT) and (B) levels of Isotocin (IT) in four treatment groups: a) sympatric cleaner (Labroides dimidiatus), b) allopatric cleaner (Elacatinus evelynae), c) conspecific (N. elegans) and d) ball, expressed as AVT (pmol/mg) and IT (pmol/mg). Medians (full lines) and interquartile range are presented in boxes.
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annual changes in brain concentration of arginine vasotocin and Isotocin correspond with phases of reproductive cycle in round goby neogobius melanostomus
Chronobiology International, 2015Co-Authors: Ewa Sokolowska, Agnieszka Kleszczynska, M Nietrzeba, Ewa KulczykowskaAbstract:Reproductive cycle of seasonally breeding fish is synchronized with changes of photoperiod and temperature in environment. We hypothesize that arginine vasotocin (AVT) and Isotocin (IT) are involved in timing and synchronization of seasonal reproductive activity in the round goby (Neogobius melanostomus). To verify this hypothesis, we examined the annual profiles of brain AVT and IT in round goby males and females in relation to their reproductive cycle. Wild round gobies were exposed to annual environmental changes in their natural habitats from where they were sampled monthly over a year. AVT and IT were measured using HPLC with fluorescence detection preceded by solid-phase extraction. This study shows seasonal variations in brain AVT and IT levels. Profiles of changes were similar in males and females: the peak of AVT was observed before spawning in March-April, whereas that of IT during spawning in May–June. Furthermore, the lowest AVT level was noted out of breeding season from November to January, ...
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neuropeptides Isotocin and arginine vasotocin in urophysis of three fish species
Fish Physiology and Biochemistry, 2013Co-Authors: Magdalena Gozdowska, Marek ślebioda, Ewa KulczykowskaAbstract:In this study, for the first time, both neuropeptides Isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish species. We have used high-performance liquid chromatographic assay with fluorescence detection (HPLC-FL) preceded by solid-phase extraction (SPE) and liquid chromatography-electrospray ionization triple-quadrupole tandem mass spectrometry (LC-ESI MS/MS) technique to determine both neuropeptides in urophysis of three fish species. The efficiency of peptide’s SPE extraction was 79–85 %. In HPLC-FL method, the limits of detection (LOD) and quantification (LOQ) were estimated as 1.0 and 3.4 pmol/mL for IT and 0.25 and 2.20 pmol/mL for AVT. In LC–MS/MS method, LOD and LOQ were estimated as 0.4 and 1.2 pmol/mL for IT and 0.06 and 0.2 pmol/mL for AVT. The chromatographic methods are good alternative for RIA, because enable to measure both nonapeptides simultaneously in one sample. In round goby (Neogobius melanostomus), three-spined stickleback (Gasterosteus aculeatus) and sea bream (Sparus aurata), urophysial IT concentrations ranged between 0.056 and 0.678 pmol/mg tissue and AVT concentrations ranged between 0.0008 (or even below detection threshold) and 0.084 pmol/mg tissue.
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stocking density influences brain arginine vasotocin avt and Isotocin it levels in males and females of three spined stickleback gasterosteus aculeatus
General and Comparative Endocrinology, 2013Co-Authors: Agnieszka Kleszczynska, Ewa KulczykowskaAbstract:Arginine vasotocin (AVT) and Isotocin (IT) are fish neurohormones produced in separate parvocellular and magnocellular preoptic neurons of Teleostei. Apart from well-established peripheral action as hormones they are important neurotransmitters in central nervous system in fish. In the present study, we examined an influence of stocking density on whole brain AVT and IT concentrations in males and females of three-spined stickleback (Gasterosteus aculeatus). In males, the highest AVT levels have been found at stocking densities of 10 and 30 individuals per 30-l tank. On the other hand, in females, AVT concentration was significantly higher in those kept alone. Brain IT concentrations significantly increased along with stocking density only in females and did not change in males. The sex-dependent responses indicate a different stimulation of AVT and IT neurons in males and females. Consequently, roles of the neurohormones in males and females exposed to stress of overcrowding must be different.
Agnieszka Kleszczynska - One of the best experts on this subject based on the ideXlab platform.
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annual changes in brain concentration of arginine vasotocin and Isotocin correspond with phases of reproductive cycle in round goby neogobius melanostomus
Chronobiology International, 2015Co-Authors: Ewa Sokolowska, Agnieszka Kleszczynska, M Nietrzeba, Ewa KulczykowskaAbstract:Reproductive cycle of seasonally breeding fish is synchronized with changes of photoperiod and temperature in environment. We hypothesize that arginine vasotocin (AVT) and Isotocin (IT) are involved in timing and synchronization of seasonal reproductive activity in the round goby (Neogobius melanostomus). To verify this hypothesis, we examined the annual profiles of brain AVT and IT in round goby males and females in relation to their reproductive cycle. Wild round gobies were exposed to annual environmental changes in their natural habitats from where they were sampled monthly over a year. AVT and IT were measured using HPLC with fluorescence detection preceded by solid-phase extraction. This study shows seasonal variations in brain AVT and IT levels. Profiles of changes were similar in males and females: the peak of AVT was observed before spawning in March-April, whereas that of IT during spawning in May–June. Furthermore, the lowest AVT level was noted out of breeding season from November to January, ...
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urotensin ii inhibits arginine vasotocin and stimulates Isotocin release from nerve endings in the pituitary of gilthead sea bream sparus aurata
Journal of Experimental Zoology, 2014Co-Authors: Hanna Kalamarzkubiak, Agnieszka Kleszczynska, Iris Meiri Ashkenazi, Hanna RosenfeldAbstract:The aim of this in vitro study was to determine whether arginine vasotocin (AVT) and Isotocin (IT) release from nerve endings is affected by urotensin II (UII) in gilthead sea bream pituitary. Primary cultures of pituitary cells were exposed to 10−12, 10−10, and 10−8 M UII for 6, 24, and 48 hr. AVT and IT contents were determined in the culture media by high performance liquid chromatography (HPLC). UII at all doses decreased AVT release after 6, 24, and 48 hr of incubation. IT release was increased by UII only after 24 hr of incubation. This study, for the first time, indicates that UII affects AVT and IT release from nerve endings in the pituitary of Sparus aurata. It is presumed that UII together with AVT and IT may control response to different salinities in fishes. J. Exp. Zool. 321A: 467–471, 2014. © 2014 Wiley Periodicals, Inc.
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in vitro effect of cortisol and urotensin i on arginine vasotocin and Isotocin secretion from pituitary cells of gilthead sea bream sparus aurata
Journal of Fish Biology, 2014Co-Authors: Hanna Kalamarzkubiak, Agnieszka Kleszczynska, I Meiriashkenazi, Hanna RosenfeldAbstract:This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and Isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4 × 10(-8) , 1·4 × 10(-7) and 0·4 × 10(-6) M cortisol and 10(-12) , 10(-10) and 10(-8) M UI for 6, 24 and 48 h, respectively. AVT and IT contents were determined in the culture media by high-performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6 h of incubation at all doses. After 24 h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic-pituitary-interrenal (HPI) axis in this species.
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stocking density influences brain arginine vasotocin avt and Isotocin it levels in males and females of three spined stickleback gasterosteus aculeatus
General and Comparative Endocrinology, 2013Co-Authors: Agnieszka Kleszczynska, Ewa KulczykowskaAbstract:Arginine vasotocin (AVT) and Isotocin (IT) are fish neurohormones produced in separate parvocellular and magnocellular preoptic neurons of Teleostei. Apart from well-established peripheral action as hormones they are important neurotransmitters in central nervous system in fish. In the present study, we examined an influence of stocking density on whole brain AVT and IT concentrations in males and females of three-spined stickleback (Gasterosteus aculeatus). In males, the highest AVT levels have been found at stocking densities of 10 and 30 individuals per 30-l tank. On the other hand, in females, AVT concentration was significantly higher in those kept alone. Brain IT concentrations significantly increased along with stocking density only in females and did not change in males. The sex-dependent responses indicate a different stimulation of AVT and IT neurons in males and females. Consequently, roles of the neurohormones in males and females exposed to stress of overcrowding must be different.
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hypothalamic arginine vasotocin and Isotocin are involved in stress response in fish
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2009Co-Authors: Ewa Kulczykowska, Magdalena Gozdowska, Agnieszka Kleszczynska, Gonzalo Martinezrodriguez, Hanna Kalamarz, M Nietrzeba, Juan Miguel ManceraAbstract:Resumen de la presentacion de un poster en: 26th Congress of the new European Society of Comparative Biochemistry and Physiology (2009, 6-9 september, Innsbruck, Austria). Session 5: The role of stress in organism performance, development and evolution.
Akihisa Urano - One of the best experts on this subject based on the ideXlab platform.
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attenuation of diurnal rhythms in plasma levels of melatonin and cortisol and hypothalamic contents of vasotocin and Isotocin mrnas in pre spawning chum salmon
General and Comparative Endocrinology, 2004Co-Authors: Daisuke Saito, Hironori Ando, Qiong Shi, Akihisa UranoAbstract:In the present study, diurnal changes in plasma levels of melatonin and cortisol, and hypothalamic contents of neurohypophysial hormone mRNAs were examined in pre-spawning chum salmon, Oncorhynchus keta. From late November to early December, homing fish were captured at two sites along their migratory pathway on the Sanriku coast, Japan. Fish captured in the seawater (SW) environment were transferred to SW aquaria, and fish captured in the freshwater (FW) environment were to FW aquaria. They were maintained under natural photoperiod of approximately 10L:14D and sacrificed at 4-h interval through 24-h period. Plasma levels of melatonin were determined by radioimmunoassay, while cortisol levels were determined by enzyme immunoassay. Hypothalamic contents of vasotocin and Isotocin mRNAs were determined by quantitative dot-blot hybridization assay. The melatonin levels showed weak nocturnal elevations in the SW and FW males, and FW females. The levels were maximal at 22:00 and minimal at 10:00 or 14:00, however the amplitudes were smaller than those reported in the previous studies using immature salmonids. The levels of vasotocin and Isotocin mRNAs were higher in the males at all time points. The mRNA levels, however, did not show any diurnal variations in either of group. The same applied to plasma cortisol levels. These results indicate that the diurnal endocrine rhythms were attenuated in pre-spawning chum salmon, in contrast to the prominent diurnal rhythms in immature salmonids.
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gonadotropin releasing hormones modulate electrical activity of vasotocin and Isotocin neurons in the brain of rainbow trout
Neuroscience Letters, 2003Co-Authors: Daisuke Saito, Yoshihisa Hasegawa, Akihisa UranoAbstract:Abstract Gonadotropin-releasing hormone (GnRH) is widely distributed in the vertebrate brains; however, its significance in the brain function is poorly understood. Both GnRH and vasopressin-family hormones are involved in control of reproductive behavior. Anatomical evidence indicated the possible action of GnRH on classical neurosecretory neurons. In the present study, we examined whether GnRH modulates electrical activity of vasotocin (VT) and Isotocin (IT) neurons in the brain of rainbow trout ( Oncorhynchus mykiss ). Two forms of GnRH, salmon GnRH and chicken GnRH II, are present in the rainbow trout brain, and their fibers are localized in the close vicinity of VT and IT neurons. Applications of both GnRH forms elevated the frequency of cell-type-specific synchronous Ca 2+ pulses in VT and IT neurons that are blocked by a GnRH-receptor antagonist. Our results showed facilitatory actions of GnRHs on VT and IT neurons, suggesting that GnRH neurons modulate classical neurosecretory neurons to control reproductive behavior.
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Synchronized Periodic Ca2+ Pulses Define Neurosecretory Activities in Magnocellular Vasotocin and Isotocin Neurons
The Journal of Neuroscience, 2001Co-Authors: Daisuke Saito, Akihisa UranoAbstract:The electrical activity of magnocellular neurosecretory cells (NSCs) is correlated with the release rates of neurohypophysial hormones. NSCs may control their secretory activity in a cooperative manner by changing their electrical activity in response to changes in the internal milieu. In the present study, we applied confocal Ca 2 imaging to a sagittally hemisected rainbow trout brain to simultaneously monitor the neuronal activity of a number of NSCs. We found that NSCs in vitro showed synchronized pulsatile elevations of intracellular Ca 2 levels at regular intervals. Double immunostaining of vasotocin (VT) and Isotocin (IT) after the confocal imaging clarified that each of the VT and IT neuronal populations showed a distinct pattern of periodic Ca 2 pulses. Simultaneous cell-attached patch recordings ensured that individual Ca 2 pulses were associated with a phasic burst firing. Depolarizing stimuli by increasing the extracellular K concentration from 5 to 7‐9 mM reversibly shortened the interpulse intervals in both VT and IT neurons. Interpulse intervals but not durations of pulses were shortened by hypo-osmotic stimuli and prolonged by hyperosmotic stimuli, consistent with the osmoregulatory function of teleost NSCs. We therefore hypothesize that NSCs use intervals of synchronized periodic burst discharges to fit the levels of secretory activity to physiological requirements.
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effects of osmotic stimulation on expression of neurohypophysial hormone genes in pre spawning chum salmon
Zoological Science, 1996Co-Authors: Hironori Ando, Susumu Hyodo, Shuichi Hiraoka, Kaoru Kubokawa, Akihisa UranoAbstract:Abstract Changes in expression of vasotocin (VT) and Isotocin (IT) genes were analyzed in chum salmon during the last stages of spawning migration. Pre-spawning chum salmon were caught at following four locations in the Sanriku coast of the Pacific Ocean in Japan: 1) the off-coast area north to the Otsuchi Bay, 2) the mouth of the Otsuchi Bay, 3) inside of the Otsuchi Bay, and 4) the place 500 m upstream to the mouth of the Otsuchi River. In addition, effects of hypo-osmotic stimulation by transition from sea water (SW) to freshwater (FW) were examined in animals caught at the mouth of the Otsuchi Bay. The levels of VT and IT mRNAs in the forebrains were determined by Northern blot analysis. The plasma osmolality and the levels of Na+ and Cl− were also analyzed. Expression patterns of VT and IT genes were different between the males and the females. In the males, VT and IT gene expression were maintained essentially at the same levels from the off-coast area to the Otsuchi River. In contrast, in the femal...
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Sexually Different Expression of Neurohypophysial Hormone Genes in the Preoptic Nucleus of Pre-Spawning Chum Salmon
Zoological Science, 1996Co-Authors: Yuki Ota, Hironori Ando, Masatoshi Ban, Hiroshi Ueda, Akihisa UranoAbstract:Abstract Vasotocin (VT) and Isotocin (IT) are teleost neurohypophysial hormones produced by neurosecretory neurons in the magnocellular part of preoptic nucleus (PM) of the hypothalamus. Several previous studies indicated that neurohypophysial hormones are involved in teleost reproductive behavior. The changes in the expression of VT and IT genes were thus studied by an in situ hybridization technique and an immunohistochemical avidin-biotin-complex method in pre-spawning chum salmon (Oncorhynchus keta). Male and female fish were caught at Atsuta, the mouth of the Ishikari Bay, and at Chitose, upstream to the Ishikari River in October, 1994. The former and the latter are referred to as bay fish and river fish, respectively. The intensity of autoradiographic hybridization signals were determined in individual neurosecretory neurons in the rostroventral, middle, and dorsocaudal parts of the PM. in the females, the levels of VT and IT mRNAs in the river fish were significantly lower than those in the bay fis...
Juan Antonio Martossitcha - One of the best experts on this subject based on the ideXlab platform.
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vasotocin and Isotocin regulate aquaporin 1 function in the sea bream
The Journal of Experimental Biology, 2015Co-Authors: Juan Antonio Martossitcha, Marco A Campinho, Juan Miguel Mancera, Gonzalo Martinezrodriguez, Juan FuentesAbstract:Aquaporins (AQPs) are specific transmembrane water channels with an important function in water homeostasis. In terrestrial vertebrates, AQP2 function is regulated by vasopressin (AVP) to accomplish key functions in osmoregulation. The endocrine control of aquaporin function in teleosts remains little studied. Therefore, in this study we investigated the regulatory role of vasotocin (AVTR) and Isotocin (ITR) receptors in Aqp1 paralog gene function in the teleost gilthead sea bream ( Sparus aurata ). The complete coding regions of Aqp1a , Aqp1b , AVTR V1a2-type , AVTR V2-type and ITR from sea bream were isolated. A Xenopus oocyte-swelling assay was used to functionally characterize AQP1 function and regulation by AVT and IT through their cognate receptors. Microinjection of oocytes with Aqp1b mRNA revealed regulation of water transport via PKA (IBMX+forskolin sensitive), whereas Aqp1a mRNA injection had the same effect via PKC signaling (PDBU sensitive). In the absence of expressed receptors, AVT and IT (10−8 mol l−1) were unable to modify AQP1 function. AVT regulated AQP1a and AQP1b function only when the AVTR V2-type was co-expressed. IT regulated AQP1a function, but not AQP1b, only when ITR was present. Considering that Aqp1a and Aqp1b gene expression in the sea bream intestine is highly salinity dependent in vivo , our results in ovo demonstrate a regulatory role for AVT and IT in AQP1 function in the sea bream in the processing of intestinal fluid to achieve osmoregulation.
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cloning of pro vasotocin and pro Isotocin in the gilthead seabream sparus aurata
2011Co-Authors: Juan Antonio Martossitcha, Juan Miguel Mancera, Gonzalo MartinezrodriguezAbstract:Trabajo presentado en el 8o Congreso de la Asociacion Iberica de Endocrinologia Comparada (AIEC), celebrado en Madrid del 5 al 7 de septiembre de 2011.
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melatonin vasotocin and Isotocin as biomarkers of the condition of fish
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2010Co-Authors: Ewa Kulczykowska, Magdalena Gozdowska, Juan Antonio Martossitcha, Juan Miguel Mancera, Hanna Kalamarz, M Nietrzeba, Gonzalo MartinezrodriguezAbstract:Trabajo resentado en el COST 867 International Workshop on Fish Welfare, celebrado en Madrid del 8 al 10 de febrero de 2011.
Magdalena Gozdowska - One of the best experts on this subject based on the ideXlab platform.
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nonapeptides arginine vasotocin and Isotocin in fishes advantage of bioactive molecules measurement
Frontiers in Marine Science, 2020Co-Authors: Ewa Sokolowska, Magdalena Gozdowska, Ewa KulczykowskaAbstract:The aim of this article is to share with fish physiologists and behaviourists our thoughts and hints regarding the involvement of nonapeptides in various physiological and behavioural processes in fishes and the advantage of measurements of bioavailable molecules. Arginine vasotocin (AVT) and Isotocin (IT) are biologically active nonapeptides in teleosts which are produced in separate neurosecretory neurons in the hypothalamic nuclei. They act as neurotransmitters and/or neuromodulators in the central nervous system (CNS) or as peripheral hormones, when they are released in the neurohypophysis and distributed via the bloodstream. Biosynthesis of bioactive AVT and IT is a complicated multistage process and nonapeptides have multiple sites of action. Different analytical approaches, including determination of pro-AVT and pro-IT mRNA in the hypothalamus or AVT and IT concentration in the brain, pituitary and plasma are used depending on the aim of the study. However, the level of biologically-active molecules is more straightforwardly related to their current action in the CNS and/or peripheral organs compared with indirect proxies of nonapeptides production such as the expression of pro-peptides mRNA. Therefore analysis of AVT and IT is highly recommended in many studies. Herein we compiled various works in which bioavailable AVT and IT were analysed in order to highlight the importance of such approach. Moreover, we compared the fluctuation of nonapeptide levels in the plasma with that in the brain of round gobies in successive reproductive phases. We also showed that neither AVT nor IT level was associated with fish age.
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Whole brain nonapeptide’ levels in clients (Naso elegans).
2017Co-Authors: Marta C. Soares, Sónia C. Cardoso, Renata Mazzei, Gonçalo I. André, Marta Morais, Magdalena Gozdowska, Hanna Kalamarz-kubiak, Ewa KulczykowskaAbstract:(A) levels of arginine vasotocin (AVT) and (B) levels of Isotocin (IT) in four treatment groups: a) sympatric cleaner (Labroides dimidiatus), b) allopatric cleaner (Elacatinus evelynae), c) conspecific (N. elegans) and d) ball, expressed as AVT (pmol/mg) and IT (pmol/mg). Medians (full lines) and interquartile range are presented in boxes.
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neuropeptides Isotocin and arginine vasotocin in urophysis of three fish species
Fish Physiology and Biochemistry, 2013Co-Authors: Magdalena Gozdowska, Marek ślebioda, Ewa KulczykowskaAbstract:In this study, for the first time, both neuropeptides Isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish species. We have used high-performance liquid chromatographic assay with fluorescence detection (HPLC-FL) preceded by solid-phase extraction (SPE) and liquid chromatography-electrospray ionization triple-quadrupole tandem mass spectrometry (LC-ESI MS/MS) technique to determine both neuropeptides in urophysis of three fish species. The efficiency of peptide’s SPE extraction was 79–85 %. In HPLC-FL method, the limits of detection (LOD) and quantification (LOQ) were estimated as 1.0 and 3.4 pmol/mL for IT and 0.25 and 2.20 pmol/mL for AVT. In LC–MS/MS method, LOD and LOQ were estimated as 0.4 and 1.2 pmol/mL for IT and 0.06 and 0.2 pmol/mL for AVT. The chromatographic methods are good alternative for RIA, because enable to measure both nonapeptides simultaneously in one sample. In round goby (Neogobius melanostomus), three-spined stickleback (Gasterosteus aculeatus) and sea bream (Sparus aurata), urophysial IT concentrations ranged between 0.056 and 0.678 pmol/mg tissue and AVT concentrations ranged between 0.0008 (or even below detection threshold) and 0.084 pmol/mg tissue.
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melatonin vasotocin and Isotocin as biomarkers of the condition of fish
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2010Co-Authors: Ewa Kulczykowska, Magdalena Gozdowska, Juan Antonio Martossitcha, Juan Miguel Mancera, Hanna Kalamarz, M Nietrzeba, Gonzalo MartinezrodriguezAbstract:Trabajo resentado en el COST 867 International Workshop on Fish Welfare, celebrado en Madrid del 8 al 10 de febrero de 2011.
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hypothalamic arginine vasotocin and Isotocin are involved in stress response in fish
Comparative Biochemistry and Physiology A-molecular & Integrative Physiology, 2009Co-Authors: Ewa Kulczykowska, Magdalena Gozdowska, Agnieszka Kleszczynska, Gonzalo Martinezrodriguez, Hanna Kalamarz, M Nietrzeba, Juan Miguel ManceraAbstract:Resumen de la presentacion de un poster en: 26th Congress of the new European Society of Comparative Biochemistry and Physiology (2009, 6-9 september, Innsbruck, Austria). Session 5: The role of stress in organism performance, development and evolution.