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Mustaque Hossain - One of the best experts on this subject based on the ideXlab platform.

  • Aggregate Retention in Chip Seal
    Transportation Research Record, 2012
    Co-Authors: Farhana Rahman, M Shahidul Islam, Haritha Y Musty, Mustaque Hossain
    Abstract:

    Chip seal is a widely used preventive maintenance treatment for flexible pavements. However, a major problem with chip seal is the damage caused by loose aggregates from newly placed seals, partly because of a lack of compatibility between aggregate and asphalt emulsion. In this study, limestone, crushed gravel, synthetic lightweight aggregates, and recycled asphalt pavement materials were studied in the laboratory with two polymer-modified asphalt emulsions to find the aggregate-emulsion combination that would result in maximum chip retention. Replicate specimens were tested in the ASTM D7000 sweep test for each aggregate-emulsion combination. Lightweight aggregates and gravel were also tested in a newly developed test setup for simulating the sweep test. Test results show that lightweight aggregates perform better than gravel. However, lightweight aggregate sources play an important role in chip retention. Emulsion is the most significant factor that affects chip loss. Statistical analysis identified th...

  • Chip Seal with Lightweight Aggregates for Low-Volume Roads
    Transportation Research Record, 2011
    Co-Authors: Shahidul Islam, Mustaque Hossain
    Abstract:

    Chip seal is widely used for preventive maintenance of low-volume roads in many states, including Kansas. Although there are no distinct design guidelines for using lightweight aggregates in chip seals, these aggregates are extensively used in Kansas to limit windshield damage caused by flying chips. Lightweight aggregates were used with polymer-modified asphalt emulsion to determine proper aggregate and emulsion application rates to minimize chip loss in longer-lasting chip seals on low-volume roads. A statistical analysis was conducted to evaluate chip seal performance characteristics that include aggregate retention, aggregate embedment depth, and rutting. Results show that aggregate retention and embedment depth depend on aggregate–emulsion interaction, whereas rutting depends on the type of aggregates used.

Xu-dong Tang - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of aggregate/aggresome structures formed by polyhedrin of Bombyx mori nucleopolyhedrovirus.
    Scientific Reports, 2015
    Co-Authors: Zhong-jian Guo, Liu-xing Tao, Xian-yun Dong, Ting Tian, Xu-dong Tang
    Abstract:

    Virus infections often lead to formation of aggregates and aggresomes in host cells. In this study, production of aggregates and aggresomes by the highly expressed protein polyhedrin of Bombyx mori nucleopolyhedrovirus (BmNPV) at 24 h postinfection (p.i.) was detected with a fluorescent molecular dye, and verified by colocalization of polyhedrin with aggresomal markers, GFP-250 and γ-tubulin. Polyhedrin aggregates showed hallmark characteristics of aggresomes: formation was microtubule-dependent; they colocalized with heat shock cognates/proteins of the 70-kDa family (HSC/HSP70s), ubiquitinated proteins and recruited the mitochondria. Aggregated polyhedrin protein gradually gained its active conformation accompanying progress of BmNPV infection. At 48 h p.i. recovered polyhedrin bound directly to Bombyx mori microtubule-associated protein 1-light chain 3 (BmLC3), an autophagosome marker, and was colocalized with BmLC3 to the isolation membrane of autophagosome, implying the involvement of polyhedrin in cellular autophagy. Inhibition of autophagy by 3-methyladenine (3-MA) dramatically resulted in decrease of polyhedrin expression and polyhedra particle production. These observations suggested that highly expressed polyhedrin forms aggregate to get involved in cellular autophagy then play an important role in polyhedra production.

  • characterization of aggregate aggresome structures formed by polyhedrin of bombyx mori nucleopolyhedrovirus
    Scientific Reports, 2015
    Co-Authors: Zhong-jian Guo, Liu-xing Tao, Xian-yun Dong, Ting Tian, Xu-dong Tang
    Abstract:

    Virus infections often lead to formation of aggregates and aggresomes in host cells. In this study, production of aggregates and aggresomes by the highly expressed protein polyhedrin of Bombyx mori nucleopolyhedrovirus (BmNPV) at 24 h postinfection (p.i.) was detected with a fluorescent molecular dye, and verified by colocalization of polyhedrin with aggresomal markers, GFP-250 and γ-tubulin. Polyhedrin aggregates showed hallmark characteristics of aggresomes: formation was microtubule-dependent; they colocalized with heat shock cognates/proteins of the 70-kDa family (HSC/HSP70s), ubiquitinated proteins and recruited the mitochondria. Aggregated polyhedrin protein gradually gained its active conformation accompanying progress of BmNPV infection. At 48 h p.i. recovered polyhedrin bound directly to Bombyx mori microtubule-associated protein 1-light chain 3 (BmLC3), an autophagosome marker, and was colocalized with BmLC3 to the isolation membrane of autophagosome, implying the involvement of polyhedrin in cellular autophagy. Inhibition of autophagy by 3-methyladenine (3-MA) dramatically resulted in decrease of polyhedrin expression and polyhedra particle production. These observations suggested that highly expressed polyhedrin forms aggregate to get involved in cellular autophagy then play an important role in polyhedra production.

Zhong-jian Guo - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of aggregate/aggresome structures formed by polyhedrin of Bombyx mori nucleopolyhedrovirus.
    Scientific Reports, 2015
    Co-Authors: Zhong-jian Guo, Liu-xing Tao, Xian-yun Dong, Ting Tian, Xu-dong Tang
    Abstract:

    Virus infections often lead to formation of aggregates and aggresomes in host cells. In this study, production of aggregates and aggresomes by the highly expressed protein polyhedrin of Bombyx mori nucleopolyhedrovirus (BmNPV) at 24 h postinfection (p.i.) was detected with a fluorescent molecular dye, and verified by colocalization of polyhedrin with aggresomal markers, GFP-250 and γ-tubulin. Polyhedrin aggregates showed hallmark characteristics of aggresomes: formation was microtubule-dependent; they colocalized with heat shock cognates/proteins of the 70-kDa family (HSC/HSP70s), ubiquitinated proteins and recruited the mitochondria. Aggregated polyhedrin protein gradually gained its active conformation accompanying progress of BmNPV infection. At 48 h p.i. recovered polyhedrin bound directly to Bombyx mori microtubule-associated protein 1-light chain 3 (BmLC3), an autophagosome marker, and was colocalized with BmLC3 to the isolation membrane of autophagosome, implying the involvement of polyhedrin in cellular autophagy. Inhibition of autophagy by 3-methyladenine (3-MA) dramatically resulted in decrease of polyhedrin expression and polyhedra particle production. These observations suggested that highly expressed polyhedrin forms aggregate to get involved in cellular autophagy then play an important role in polyhedra production.

  • characterization of aggregate aggresome structures formed by polyhedrin of bombyx mori nucleopolyhedrovirus
    Scientific Reports, 2015
    Co-Authors: Zhong-jian Guo, Liu-xing Tao, Xian-yun Dong, Ting Tian, Xu-dong Tang
    Abstract:

    Virus infections often lead to formation of aggregates and aggresomes in host cells. In this study, production of aggregates and aggresomes by the highly expressed protein polyhedrin of Bombyx mori nucleopolyhedrovirus (BmNPV) at 24 h postinfection (p.i.) was detected with a fluorescent molecular dye, and verified by colocalization of polyhedrin with aggresomal markers, GFP-250 and γ-tubulin. Polyhedrin aggregates showed hallmark characteristics of aggresomes: formation was microtubule-dependent; they colocalized with heat shock cognates/proteins of the 70-kDa family (HSC/HSP70s), ubiquitinated proteins and recruited the mitochondria. Aggregated polyhedrin protein gradually gained its active conformation accompanying progress of BmNPV infection. At 48 h p.i. recovered polyhedrin bound directly to Bombyx mori microtubule-associated protein 1-light chain 3 (BmLC3), an autophagosome marker, and was colocalized with BmLC3 to the isolation membrane of autophagosome, implying the involvement of polyhedrin in cellular autophagy. Inhibition of autophagy by 3-methyladenine (3-MA) dramatically resulted in decrease of polyhedrin expression and polyhedra particle production. These observations suggested that highly expressed polyhedrin forms aggregate to get involved in cellular autophagy then play an important role in polyhedra production.

Shahidul Islam - One of the best experts on this subject based on the ideXlab platform.

  • Chip Seal with Lightweight Aggregates for Low-Volume Roads
    Transportation Research Record, 2011
    Co-Authors: Shahidul Islam, Mustaque Hossain
    Abstract:

    Chip seal is widely used for preventive maintenance of low-volume roads in many states, including Kansas. Although there are no distinct design guidelines for using lightweight aggregates in chip seals, these aggregates are extensively used in Kansas to limit windshield damage caused by flying chips. Lightweight aggregates were used with polymer-modified asphalt emulsion to determine proper aggregate and emulsion application rates to minimize chip loss in longer-lasting chip seals on low-volume roads. A statistical analysis was conducted to evaluate chip seal performance characteristics that include aggregate retention, aggregate embedment depth, and rutting. Results show that aggregate retention and embedment depth depend on aggregate–emulsion interaction, whereas rutting depends on the type of aggregates used.

  • Evaluation of lightweight aggregates in chip seal
    2010
    Co-Authors: Shahidul Islam
    Abstract:

    Chip seal, also known as seal coat, is widely used as a low-cost, thin surface treatment in preventive maintenance of asphalt pavements. Loosening of aggregate particles from chip-sealed pavement and associated windshield damage of vehicle is a common problem. Thus Kansas Department of Transportation uses lightweight aggregates as cover materials for chip seals. Although this has decreased wind shield damage problems, extensive chip loss on seal-coated pavements in the state has been reported. In this project, lightweight aggregates were studied in the laboratory to determine the effect of moisture content and electrical charge on chip loss. Results indicate that retention of aggregated depends upon the prevailing charges of aggregate and emulsion particles. Moisture condition of the aggregates does not have any effect on chip loss. A new sweep test machine has been developed to assess chip loss and it was found to be better than the sweep test recommended by the American Society for Testing and Materials (ASTM).

Ting Tian - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of aggregate/aggresome structures formed by polyhedrin of Bombyx mori nucleopolyhedrovirus.
    Scientific Reports, 2015
    Co-Authors: Zhong-jian Guo, Liu-xing Tao, Xian-yun Dong, Ting Tian, Xu-dong Tang
    Abstract:

    Virus infections often lead to formation of aggregates and aggresomes in host cells. In this study, production of aggregates and aggresomes by the highly expressed protein polyhedrin of Bombyx mori nucleopolyhedrovirus (BmNPV) at 24 h postinfection (p.i.) was detected with a fluorescent molecular dye, and verified by colocalization of polyhedrin with aggresomal markers, GFP-250 and γ-tubulin. Polyhedrin aggregates showed hallmark characteristics of aggresomes: formation was microtubule-dependent; they colocalized with heat shock cognates/proteins of the 70-kDa family (HSC/HSP70s), ubiquitinated proteins and recruited the mitochondria. Aggregated polyhedrin protein gradually gained its active conformation accompanying progress of BmNPV infection. At 48 h p.i. recovered polyhedrin bound directly to Bombyx mori microtubule-associated protein 1-light chain 3 (BmLC3), an autophagosome marker, and was colocalized with BmLC3 to the isolation membrane of autophagosome, implying the involvement of polyhedrin in cellular autophagy. Inhibition of autophagy by 3-methyladenine (3-MA) dramatically resulted in decrease of polyhedrin expression and polyhedra particle production. These observations suggested that highly expressed polyhedrin forms aggregate to get involved in cellular autophagy then play an important role in polyhedra production.

  • characterization of aggregate aggresome structures formed by polyhedrin of bombyx mori nucleopolyhedrovirus
    Scientific Reports, 2015
    Co-Authors: Zhong-jian Guo, Liu-xing Tao, Xian-yun Dong, Ting Tian, Xu-dong Tang
    Abstract:

    Virus infections often lead to formation of aggregates and aggresomes in host cells. In this study, production of aggregates and aggresomes by the highly expressed protein polyhedrin of Bombyx mori nucleopolyhedrovirus (BmNPV) at 24 h postinfection (p.i.) was detected with a fluorescent molecular dye, and verified by colocalization of polyhedrin with aggresomal markers, GFP-250 and γ-tubulin. Polyhedrin aggregates showed hallmark characteristics of aggresomes: formation was microtubule-dependent; they colocalized with heat shock cognates/proteins of the 70-kDa family (HSC/HSP70s), ubiquitinated proteins and recruited the mitochondria. Aggregated polyhedrin protein gradually gained its active conformation accompanying progress of BmNPV infection. At 48 h p.i. recovered polyhedrin bound directly to Bombyx mori microtubule-associated protein 1-light chain 3 (BmLC3), an autophagosome marker, and was colocalized with BmLC3 to the isolation membrane of autophagosome, implying the involvement of polyhedrin in cellular autophagy. Inhibition of autophagy by 3-methyladenine (3-MA) dramatically resulted in decrease of polyhedrin expression and polyhedra particle production. These observations suggested that highly expressed polyhedrin forms aggregate to get involved in cellular autophagy then play an important role in polyhedra production.