The Experts below are selected from a list of 27 Experts worldwide ranked by ideXlab platform

Sudeshna Saha - One of the best experts on this subject based on the ideXlab platform.

  • High-temperature tolerant enzyme based extraction of lignocellulose derived C5 and C6 sugars for the integrated production of a promising supplement for a diesel/Jet-Engine fuel
    Fuel, 2020
    Co-Authors: Sibashish Baksi, Ujjaini Sarkar, Sneha Mukherjee, Jagdish Chandra Kuniyal, Sudeshna Saha
    Abstract:

    Abstract Development of a novel, integrated production of C8 ester butyl butyrate (BuB), a promising supplement for diesel/Jet-Engine Fuels, from lignocellulose derived C5 and C6 sugars is attempted by means of smart and innovative process design. In the pathway, enzymatic hydrolysis of biomass with high solid loading is an important step which requires reduced amount of water supply and energy input. However, increased substrate loading lead to diffusion limitations of enzymes, on top of nonproductive absorption of enzymes. Addition of s-glucosidase over cellulase is another reason to increase the cost of enzymes. Viscozyme can be considered as a troubleshooter in these cases where it can offset high substrate loading and increased monomeric sugar generation. In the present study, 17.61 g/L of glucose, along with 7.43 g/L of xylose, have been recovered with a viscozyme concentration of 54.4 g/L. Additionally, thermal deactivation of viscozyme has been investigated. The deactivation constant is found to be gradually increasing with escalated enzyme loading from 24.2 g/L to 54.4 g/L. In this study, it has been found that hydrolysis of biomass follows a first order pseudo-kinetics and the same is eventually used to determine the fractal kinetics of cellulose hydrolysis. Fractal kinetics showed that increment of enzyme concentration is associated with lower fractal dimensions and increased binding capacity of substrate and enzyme. Diffusion limitation of the present enzyme-substrate system has also been investigated. It is observed that faster diffusion, along with increased resistances, can be achieved with increased enzyme concentration.

  • Anaerobic Bio-reactor Modeling
    Computer Aided Chemical Engineering, 2016
    Co-Authors: Cansu Birgen, Heinz A. Preisig, Alexander Wentzel, Sidsel Markussen, Bernd Wittgens, Ujjaini Sarkar, Anindita Ganguly, Sudeshna Saha, Sibashish Baksi
    Abstract:

    Abstract EcoLodge aims at providing a proof-of-concept for a new, integrated biotechnological production process for C8 ester butyl butyrate, a promising supplement/substitute for diesel and Jet Engine Fuels, from lignocellulose derived C5 and C6 sugars. Butanol and butyric acid are the process intermediates produced via anaerobic fermentation. This paper focuses on mathematical model simulation for BuOH fermentation and integrated gas stripping unit. Inhibitory effects of butanol and substrate are identified as main obstacles for high productivity butanol fermentation. Therefore, in situ removal of inhibitory butanol from the bio-reactor by gas stripping is suggested taking the substrate inhibition into consideration. Mathematical models from the literature are employed together with experimentally estimated model parameters. Model simulations of the integrated unit are performed in continuous mode for determination of optimum values for substrate concentration in feed and feed flow rate. The full domain of the operating ranges were explored for three different inhibitory concentrations of butanol (5,8 and 10 g/l), which resulted in performing 14000 + simulation experiments. The results provide a clear insight into the feasible combinations of the operating conditions. The optimal operating range for 5 g/l is obtained in this study will be employed in experimental bio-reactor and gas stripping unit in the next phase of the project.

Sibashish Baksi - One of the best experts on this subject based on the ideXlab platform.

  • High-temperature tolerant enzyme based extraction of lignocellulose derived C5 and C6 sugars for the integrated production of a promising supplement for a diesel/Jet-Engine fuel
    Fuel, 2020
    Co-Authors: Sibashish Baksi, Ujjaini Sarkar, Sneha Mukherjee, Jagdish Chandra Kuniyal, Sudeshna Saha
    Abstract:

    Abstract Development of a novel, integrated production of C8 ester butyl butyrate (BuB), a promising supplement for diesel/Jet-Engine Fuels, from lignocellulose derived C5 and C6 sugars is attempted by means of smart and innovative process design. In the pathway, enzymatic hydrolysis of biomass with high solid loading is an important step which requires reduced amount of water supply and energy input. However, increased substrate loading lead to diffusion limitations of enzymes, on top of nonproductive absorption of enzymes. Addition of s-glucosidase over cellulase is another reason to increase the cost of enzymes. Viscozyme can be considered as a troubleshooter in these cases where it can offset high substrate loading and increased monomeric sugar generation. In the present study, 17.61 g/L of glucose, along with 7.43 g/L of xylose, have been recovered with a viscozyme concentration of 54.4 g/L. Additionally, thermal deactivation of viscozyme has been investigated. The deactivation constant is found to be gradually increasing with escalated enzyme loading from 24.2 g/L to 54.4 g/L. In this study, it has been found that hydrolysis of biomass follows a first order pseudo-kinetics and the same is eventually used to determine the fractal kinetics of cellulose hydrolysis. Fractal kinetics showed that increment of enzyme concentration is associated with lower fractal dimensions and increased binding capacity of substrate and enzyme. Diffusion limitation of the present enzyme-substrate system has also been investigated. It is observed that faster diffusion, along with increased resistances, can be achieved with increased enzyme concentration.

  • Anaerobic Bio-reactor Modeling
    Computer Aided Chemical Engineering, 2016
    Co-Authors: Cansu Birgen, Heinz A. Preisig, Alexander Wentzel, Sidsel Markussen, Bernd Wittgens, Ujjaini Sarkar, Anindita Ganguly, Sudeshna Saha, Sibashish Baksi
    Abstract:

    Abstract EcoLodge aims at providing a proof-of-concept for a new, integrated biotechnological production process for C8 ester butyl butyrate, a promising supplement/substitute for diesel and Jet Engine Fuels, from lignocellulose derived C5 and C6 sugars. Butanol and butyric acid are the process intermediates produced via anaerobic fermentation. This paper focuses on mathematical model simulation for BuOH fermentation and integrated gas stripping unit. Inhibitory effects of butanol and substrate are identified as main obstacles for high productivity butanol fermentation. Therefore, in situ removal of inhibitory butanol from the bio-reactor by gas stripping is suggested taking the substrate inhibition into consideration. Mathematical models from the literature are employed together with experimentally estimated model parameters. Model simulations of the integrated unit are performed in continuous mode for determination of optimum values for substrate concentration in feed and feed flow rate. The full domain of the operating ranges were explored for three different inhibitory concentrations of butanol (5,8 and 10 g/l), which resulted in performing 14000 + simulation experiments. The results provide a clear insight into the feasible combinations of the operating conditions. The optimal operating range for 5 g/l is obtained in this study will be employed in experimental bio-reactor and gas stripping unit in the next phase of the project.

Jagdish Chandra Kuniyal - One of the best experts on this subject based on the ideXlab platform.

  • High-temperature tolerant enzyme based extraction of lignocellulose derived C5 and C6 sugars for the integrated production of a promising supplement for a diesel/Jet-Engine fuel
    Fuel, 2020
    Co-Authors: Sibashish Baksi, Ujjaini Sarkar, Sneha Mukherjee, Jagdish Chandra Kuniyal, Sudeshna Saha
    Abstract:

    Abstract Development of a novel, integrated production of C8 ester butyl butyrate (BuB), a promising supplement for diesel/Jet-Engine Fuels, from lignocellulose derived C5 and C6 sugars is attempted by means of smart and innovative process design. In the pathway, enzymatic hydrolysis of biomass with high solid loading is an important step which requires reduced amount of water supply and energy input. However, increased substrate loading lead to diffusion limitations of enzymes, on top of nonproductive absorption of enzymes. Addition of s-glucosidase over cellulase is another reason to increase the cost of enzymes. Viscozyme can be considered as a troubleshooter in these cases where it can offset high substrate loading and increased monomeric sugar generation. In the present study, 17.61 g/L of glucose, along with 7.43 g/L of xylose, have been recovered with a viscozyme concentration of 54.4 g/L. Additionally, thermal deactivation of viscozyme has been investigated. The deactivation constant is found to be gradually increasing with escalated enzyme loading from 24.2 g/L to 54.4 g/L. In this study, it has been found that hydrolysis of biomass follows a first order pseudo-kinetics and the same is eventually used to determine the fractal kinetics of cellulose hydrolysis. Fractal kinetics showed that increment of enzyme concentration is associated with lower fractal dimensions and increased binding capacity of substrate and enzyme. Diffusion limitation of the present enzyme-substrate system has also been investigated. It is observed that faster diffusion, along with increased resistances, can be achieved with increased enzyme concentration.

Sneha Mukherjee - One of the best experts on this subject based on the ideXlab platform.

  • High-temperature tolerant enzyme based extraction of lignocellulose derived C5 and C6 sugars for the integrated production of a promising supplement for a diesel/Jet-Engine fuel
    Fuel, 2020
    Co-Authors: Sibashish Baksi, Ujjaini Sarkar, Sneha Mukherjee, Jagdish Chandra Kuniyal, Sudeshna Saha
    Abstract:

    Abstract Development of a novel, integrated production of C8 ester butyl butyrate (BuB), a promising supplement for diesel/Jet-Engine Fuels, from lignocellulose derived C5 and C6 sugars is attempted by means of smart and innovative process design. In the pathway, enzymatic hydrolysis of biomass with high solid loading is an important step which requires reduced amount of water supply and energy input. However, increased substrate loading lead to diffusion limitations of enzymes, on top of nonproductive absorption of enzymes. Addition of s-glucosidase over cellulase is another reason to increase the cost of enzymes. Viscozyme can be considered as a troubleshooter in these cases where it can offset high substrate loading and increased monomeric sugar generation. In the present study, 17.61 g/L of glucose, along with 7.43 g/L of xylose, have been recovered with a viscozyme concentration of 54.4 g/L. Additionally, thermal deactivation of viscozyme has been investigated. The deactivation constant is found to be gradually increasing with escalated enzyme loading from 24.2 g/L to 54.4 g/L. In this study, it has been found that hydrolysis of biomass follows a first order pseudo-kinetics and the same is eventually used to determine the fractal kinetics of cellulose hydrolysis. Fractal kinetics showed that increment of enzyme concentration is associated with lower fractal dimensions and increased binding capacity of substrate and enzyme. Diffusion limitation of the present enzyme-substrate system has also been investigated. It is observed that faster diffusion, along with increased resistances, can be achieved with increased enzyme concentration.

Ujjaini Sarkar - One of the best experts on this subject based on the ideXlab platform.

  • High-temperature tolerant enzyme based extraction of lignocellulose derived C5 and C6 sugars for the integrated production of a promising supplement for a diesel/Jet-Engine fuel
    Fuel, 2020
    Co-Authors: Sibashish Baksi, Ujjaini Sarkar, Sneha Mukherjee, Jagdish Chandra Kuniyal, Sudeshna Saha
    Abstract:

    Abstract Development of a novel, integrated production of C8 ester butyl butyrate (BuB), a promising supplement for diesel/Jet-Engine Fuels, from lignocellulose derived C5 and C6 sugars is attempted by means of smart and innovative process design. In the pathway, enzymatic hydrolysis of biomass with high solid loading is an important step which requires reduced amount of water supply and energy input. However, increased substrate loading lead to diffusion limitations of enzymes, on top of nonproductive absorption of enzymes. Addition of s-glucosidase over cellulase is another reason to increase the cost of enzymes. Viscozyme can be considered as a troubleshooter in these cases where it can offset high substrate loading and increased monomeric sugar generation. In the present study, 17.61 g/L of glucose, along with 7.43 g/L of xylose, have been recovered with a viscozyme concentration of 54.4 g/L. Additionally, thermal deactivation of viscozyme has been investigated. The deactivation constant is found to be gradually increasing with escalated enzyme loading from 24.2 g/L to 54.4 g/L. In this study, it has been found that hydrolysis of biomass follows a first order pseudo-kinetics and the same is eventually used to determine the fractal kinetics of cellulose hydrolysis. Fractal kinetics showed that increment of enzyme concentration is associated with lower fractal dimensions and increased binding capacity of substrate and enzyme. Diffusion limitation of the present enzyme-substrate system has also been investigated. It is observed that faster diffusion, along with increased resistances, can be achieved with increased enzyme concentration.

  • Anaerobic Bio-reactor Modeling
    Computer Aided Chemical Engineering, 2016
    Co-Authors: Cansu Birgen, Heinz A. Preisig, Alexander Wentzel, Sidsel Markussen, Bernd Wittgens, Ujjaini Sarkar, Anindita Ganguly, Sudeshna Saha, Sibashish Baksi
    Abstract:

    Abstract EcoLodge aims at providing a proof-of-concept for a new, integrated biotechnological production process for C8 ester butyl butyrate, a promising supplement/substitute for diesel and Jet Engine Fuels, from lignocellulose derived C5 and C6 sugars. Butanol and butyric acid are the process intermediates produced via anaerobic fermentation. This paper focuses on mathematical model simulation for BuOH fermentation and integrated gas stripping unit. Inhibitory effects of butanol and substrate are identified as main obstacles for high productivity butanol fermentation. Therefore, in situ removal of inhibitory butanol from the bio-reactor by gas stripping is suggested taking the substrate inhibition into consideration. Mathematical models from the literature are employed together with experimentally estimated model parameters. Model simulations of the integrated unit are performed in continuous mode for determination of optimum values for substrate concentration in feed and feed flow rate. The full domain of the operating ranges were explored for three different inhibitory concentrations of butanol (5,8 and 10 g/l), which resulted in performing 14000 + simulation experiments. The results provide a clear insight into the feasible combinations of the operating conditions. The optimal operating range for 5 g/l is obtained in this study will be employed in experimental bio-reactor and gas stripping unit in the next phase of the project.