The Experts below are selected from a list of 1674 Experts worldwide ranked by ideXlab platform
Bahri Patir - One of the best experts on this subject based on the ideXlab platform.
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Polymorphisms of Kappa-Casein gene and their effects on milk production traits in Holstein, Jersey and Brown Swiss cattle*
Animal Production Science, 2018Co-Authors: Murad Gurses, Huseyin Yuce, Ebru Onalan Etem, Bahri PatirAbstract:The objectives of this study were to detect Kappa-Casein (κ-CN, CSN3, CASK) gene polymorphisms and to investigate the association between the κ-CN gene polymorphisms and milk yield and composition in cattle. For this purpose, a total of 365 blood and milk samples were collected from Holstein, Jersey and Brown Swiss cows. Polymorphisms of κ-CN gene were detected by the PCR and restriction fragment length polymorphism. A 776-bp fragment of exon IV was amplified by PCR and digested with HindIII and HaeIII restriction endonuclease to distinguish allele A, B and E. The analysis of associations between κ-CN genotypes and milk yield and composition were conducted with the use of the general linear model procedure. As a result, allele frequencies were detected as 0.736, 0.357, 0.308 for A allele, 0.186, 0.643, 0.692 for B allele in Holstein, Brown Swiss and Jersey breeds, respectively, and 0.079 for E allele in Holstein. This study also demonstrated the presence of E allele in Turkish Holstein. Although κ-CN genotypes were associated with protein and solids-not-fat content of milk in Hostein and Brown Swiss, they were associated with milk fat content in Jerseys (P < 0.05).
Peter Dovc - One of the best experts on this subject based on the ideXlab platform.
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polymorphisms in the Kappa Casein csn3 gene in horse and comparative analysis of its promoter and coding region
Animal Genetics, 2008Co-Authors: S Hobor, Tanja Kunej, Peter DovcAbstract:The major parts of the coding region and promoter of the equine Kappa Casein (CSN3) gene were sequenced and compared among several species. Four SNPs were identified in the CSN3 gene: two in exon 1 and two in exon 4. The SNPs were genotyped in six Slovenian horse breeds using RFLP and two different PCR-based methods. The highest variation in genotype frequencies was found in the Slovenian cold-blood breed. The SNPs in exon 4 may cause a change in the amino acid sequence and may alter chemical/functional properties of the protein. Using horse-specific primers, we obtained 400 bp of exon 4 sequence from zebra and donkey. Two SNPs within the zebra exon 4 sequence were discovered; both presumably caused amino acid substitutions. Within the equine promoter sequence, 15 SNPs were found and 12 of them could be involved in the gain/loss of potential transcription factor (TF) binding sites. Using a comparative genomics approach, we obtained 1482 bp of the promoter sequence from zebra and donkey. Sequence alignment revealed highly conserved blocks of promoter sequence among nine species (sheep, goat, cow, zebra, donkey, horse, chimp, macaque and human) and clustered these species in three distinct groups. Consensus binding sites for TFs STAT5, C/EBP, NF1 and STAT6, previously demonstrated to be associated with expression, were located within conserved regions. Four promoter regions were tested for specific binding of TFs using electrophoretic mobility shift assays. Predicted binding sites for C/EBP and NF1 were confirmed and one conserved region was specifically detected by a yet-uncharacterized TF.
Murad Gurses - One of the best experts on this subject based on the ideXlab platform.
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Polymorphisms of Kappa-Casein gene and their effects on milk production traits in Holstein, Jersey and Brown Swiss cattle*
Animal Production Science, 2018Co-Authors: Murad Gurses, Huseyin Yuce, Ebru Onalan Etem, Bahri PatirAbstract:The objectives of this study were to detect Kappa-Casein (κ-CN, CSN3, CASK) gene polymorphisms and to investigate the association between the κ-CN gene polymorphisms and milk yield and composition in cattle. For this purpose, a total of 365 blood and milk samples were collected from Holstein, Jersey and Brown Swiss cows. Polymorphisms of κ-CN gene were detected by the PCR and restriction fragment length polymorphism. A 776-bp fragment of exon IV was amplified by PCR and digested with HindIII and HaeIII restriction endonuclease to distinguish allele A, B and E. The analysis of associations between κ-CN genotypes and milk yield and composition were conducted with the use of the general linear model procedure. As a result, allele frequencies were detected as 0.736, 0.357, 0.308 for A allele, 0.186, 0.643, 0.692 for B allele in Holstein, Brown Swiss and Jersey breeds, respectively, and 0.079 for E allele in Holstein. This study also demonstrated the presence of E allele in Turkish Holstein. Although κ-CN genotypes were associated with protein and solids-not-fat content of milk in Hostein and Brown Swiss, they were associated with milk fat content in Jerseys (P < 0.05).
C Boscos - One of the best experts on this subject based on the ideXlab platform.
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effect of Kappa Casein and beta lactoglobulin loci on milk production traits and reproductive performance of holstein cows
Journal of Dairy Science, 2005Co-Authors: A Tsiaras, G G Bargouli, Georgios Banos, C BoscosAbstract:The effects of Kappa-Casein (Kappa-CN) and beta-lactoglobulin (beta-LG) loci on milk production traits (milk, fat, protein, and lactose yield, fat, protein, and lactose content) and reproductive performance (gestation length, calving interval, age at first and second calving, number of services per conception) was estimated for 278 Holstein cows in the first 2 lactations. Genotypes of Kappa-CN and beta-LG were determined by alkaline and acidic polyacrylamide gel electrophoresis. Milk production was recorded daily. Single-trait, mixed, linear models were used for the statistical analysis of the data. Results indicated that Kappa-CN genotypes affected significantly protein yield and content (genotype AB > genotype AA). A tendency for increased milk and fat yield of animals having AB Kappa-CN genotype was also found. Fat content and lactose yield and content were not affected. In the beta-LG system, significant differences were detected for milk yield (AB > AA), fat yield (BB and AB > AA), fat content (BB > AA and AB), and lactose yield (AB > AA). A tendency for higher protein yield was also observed (AB > AA). The beta-LG locus had no significant effect on protein and lactose content. No associations between polymorphisms at the Kappa-CN locus and reproductive performance were found. There was a tendency, however, for cows with AB genotype to have older age at first and second calving. In the beta-LG system, cows with AA genotype had significantly shorter gestation length than did those with AB or BB genotype. No differences were detected between beta-LG polymorphisms for the other reproductive traits.
Cairns, Michael T. - One of the best experts on this subject based on the ideXlab platform.
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Bovine Kappa-Casein gene promoter haplotypes with potential implications for milk protein expression
Elsevier ScienceDirect, 2016Co-Authors: Keating A. F., Davoren P., Smith T. J., Cairns, Michael T.Abstract:Journal articleGenetic analysis of the Kappa-Casein gene (CSN3) promoter regions of 42 cattle representing 9 different breeds revealed that 2 distinct haplotypes (A and B) exist at this locus, differing from each other by single base changes at positions -514 (T/G), -426 (T/C), and -384 ( T/C), where haplotype A has bases T, T, and T and haplotype B has bases G, C, and C. The AA and AB haplotypes were found to occur at a higher frequency in the animals tested, with 69.0 and 21.4% being homozygous and heterozygous, respectively. The sequences that include these polymorphisms are potentially important in transcriptional regulation of the Kappa-Casein gene, because they contain putative sites for binding of many transcription factors. Linkage disequilibrium between the Kappa-Casein promoter haplotype and either one of the 2 major Kappa-Casein coding sequence haplotypes was not evident. The A allele is dominant in all groups (dairy, beef, and dual purpose) with an allele frequency of 80% and is higher among high-yielding dairy animals (88.9%) than among beef animals (75%). The AB haplotype is comparatively rare in the dairy cattle (11.1%) compared with both beef and dual-purpose animals. The BB haplotype, though rare overall (9.5%), is much higher in dual-purpose animals (18.8%) than dairy (5.6%) animals. In contrast, the B allele is much more representative of the Kappa-Casein promoters from other ruminants.Enterprise Ireland; Teagasc Walsh fellowshi
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Bovine Kappa-Casein gene promoter haplotypes with potential implications for milk protein expression
'American Dairy Science Association', 2016Co-Authors: Keating A. F., Davoren P., Smith T. J., Cairns, Michael T.Abstract:Journal articleGenetic analysis of the Kappa-Casein gene (CSN3) promoter regions of 42 cattle representing 9 different breeds revealed that 2 distinct haplotypes (A and B) exist at this locus, differing from each other by single base changes at positions -514 (T/G), -426 (T/C), and -384 ( T/C), where haplotype A has bases T, T, and T and haplotype B has bases G, C, and C. The AA and AB haplotypes were found to occur at a higher frequency in the animals tested, with 69.0 and 21.4% being homozygous and heterozygous, respectively. The sequences that include these polymorphisms are potentially important in transcriptional regulation of the Kappa-Casein gene, because they contain putative sites for binding of many transcription factors. Linkage disequilibrium between the Kappa-Casein promoter haplotype and either one of the 2 major Kappa-Casein coding sequence haplotypes was not evident. The A allele is dominant in all groups (dairy, beef, and dual purpose) with an allele frequency of 80% and is higher among high-yielding dairy animals (88.9%) than among beef animals (75%). The AB haplotype is comparatively rare in the dairy cattle (11.1%) compared with both beef and dual-purpose animals. The BB haplotype, though rare overall (9.5%), is much higher in dual-purpose animals (18.8%) than dairy (5.6%) animals. In contrast, the B allele is much more representative of the Kappa-Casein promoters from other ruminants.Enterprise Ireland; Teagasc Walsh fellowshippeer-reviewe
