The Experts below are selected from a list of 153 Experts worldwide ranked by ideXlab platform
Chen Xi - One of the best experts on this subject based on the ideXlab platform.
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combined detection of anti cyclic citrullinated peptide anti Keratin Antibody and rheumatoid factor in the diagnosis of rheumatoid arthritis
Journal of Shanxi Medical University, 2010Co-Authors: Chen XiAbstract:Objective To compare the sensitivity and specificity of anti-cyclic citrullinated peptide Antibody ( CCP) ,anti-Keratin Antibody( AKA) and rheumatoid factor ( RF) in the diagnosis of rheumatoid arthritis ( RA) ,and to explore the clinical value of combined detection of the three auto-antibodies. Methods Serum levels of anti-CCP,AKA and RF were detected in 184 RA patients and 118 patients with other autoimmune diseases using ELISA,indirect immunofluorescence and nephelometric assay,respectively. Results The sensitivities of anti-CCP,AKA and RF were respectively 78. 5% ,23. 9% and 67. 4% ,and the specificities were respectively 90. 6% ,90. 7% and 72. 9% . The areas under ROC curve of RF and anti-CCP were respectively 0. 684 and 0. 772( P 0. 01) . The positive rates of the three auto-antibodies in RA patients were significantly higher than in non-RA patients ( P 0. 05) . The sensitivity of combined detection of the three auto-antibodies was up to 94. 1% . Conclusion For the three antibodies,anti-CCP has the highest sensitivity for diagnosing RA,while AKA has the highest specificity. Combined detection of the three auto-antibodies will improve the sensitivity in the diagnosis of RA.
G E Sale - One of the best experts on this subject based on the ideXlab platform.
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positive identification of enterocytes by Keratin Antibody staining of sloughed intestinal tissue in severe gvhd
Bone Marrow Transplantation, 1993Co-Authors: M R R Silva, K Henne, G E SaleAbstract:Nine marrow allograft recipients who developed GVHD of the gastrointestinal tract accompanied by the passage of ropey necrotic material per rectum were studied. The material, resembling necrotic intestinal mucosa, was evaluated for the presence of epithelial cells using monoclonal antibodies for Keratin and macrophages. Two Keratins (AE1/AE3 and 34βE12) were detected within cells in all cases while macrophages were found in all but one case. In three cases, some cells were positive for both antibodies suggesting the presence of phagocytosed Keratin in macrophage cytoplasm. Search for organisms with Gram and methenamine silver stains showed bacteria in six cases and fungus in one
Jingang An - One of the best experts on this subject based on the ideXlab platform.
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complement activation contributes to the anti methicillin resistant staphylococcus aureus effect of natural anti Keratin Antibody
Biochemical and Biophysical Research Communications, 2015Co-Authors: Jingang An, Zhengxiao Li, Yingying Dong, Jiawen WuAbstract:Methicillin-resistant Staphylococcus aureus (MRSA) remains a major public health problem worldwide because of its strong resistance to a variety of antibiotics. Natural immunoglobulin (Ig) M antibodies have been reported to protect against microbial infections. In the present study, the function of a monoclonal natural anti-Keratin Antibody IgM (named 3B4) in MRSA infection was evaluated. The binding of 3B4 to MRSA was studied using immunofluorescence assay and flow cytometry (FCM). The binding of 3B4 to mannose-binding lectin (MBL) and complement activation were detected by ELISA. For the in vivo study, transgenic mice for the VH gene from 3B4 (TgVH 3B4) were used. After infection, the bacterial burden was examined in the kidney, spleen and enterocelia. Inflammatory cytokine levels and the neutrophil ratio in peritoneal lavage fluid (PLF) were assessed by ELISA and FCM, respectively. Additionally, the total serum hemolytic activity (CH50) in the early stage of infection was detected by ELISA. The results showed that 3B4 bound directly to MRSA and MBL, and the interaction between 3B4 and MRSA/MBL led to the activation of the classic and the MBL pathway in vitro. After 48 h of MRSA infection, the bacterial load in the kidney, spleen and enterocelia was significantly decreased in TgVH 3B4 mice (P < 0.05) compared with wild-type mice. Levels of IL-6, TNF-α, and IFN-γ were increased after MRSA infection. The levels of IL-6 and TNF-α in TgVH 3B4 mice were decreased by 49.1% and 59.4% compared to wild-type mice. Additionally, the neutrophil ratio in the PLF of TgVH 3B4 mice was decreased by 65.9%. The CH50 value was significantly higher in TgVH 3B4 mice than in wild-type mice, indicating that 3B4 promoted the activation of the complement system in MRSA infected mice. The results reveal an important role of 3B4 in the anti-MRSA immune response, and the complement activation contributes to this effect.
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host defence against c albicans infections in igh transgenic mice with vh derived from a natural anti Keratin Antibody
Cellular Microbiology, 2007Co-Authors: Wei Li, Jingang An, Meng Fu, Ying Xing, Ping Zhang, Xin Zhang, Yaochun Wang, Chengxin Li, Rong Tian, Wenjing SuAbstract:Summary Fungal infections have been increasing and life-threatening in recent years, but host immune responses, especially the humoral immunity, to fungi have not been fully understood. In the present study, we report that natural antibodies from unimmunized mice bind to Candida albicans. We established a monoclonal natural Antibody, 3B4, which recognized a surface antigen located at germ tubes of C. albicans. The 3B4 Antibody protected mice from C. albicans-induced death in passive immunization, by mechanisms involving suppressing germ tube formation and modulating phagocytosis. Interestingly, 3B4 also bound to a self-antigen Keratin. To further study the generation and anti-C. albicans activities of natural antibodies in vivo, we constructed a μ chain transgenic mouse (TgVH3B4) using the VH gene from 3B4. TgVH3B4 had elevated serum anti-Keratin/C. albicans IgM, and were resistant to C. albicans infections. Analyses of B cell development showed that in TgVH3B4, B cells secreting the anti-Keratin/C. albicans antibodies were enriched in the B1 B cell compartment. Our findings reveal an important role of Keratin-reactive natural antibodies in anti-C. albicans immune responses, and suggest that Keratin may function in selecting B cells into the B1 B cell compartment, where natural antibodies are made to fight fungal infections.
Mei Yifang - One of the best experts on this subject based on the ideXlab platform.
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clinical significance of anti cyclic citrullinated peptide anti Keratin Antibody rheumatoid factor and glucose 6 phosphate isomerase in detection of rheumatoid arthritis
Journal of Harbin Medical University, 2012Co-Authors: Mei YifangAbstract:Objective To compare the sensitivity and specificity of anti-cyclic citrullinated peptide(CCP) Antibody,anti-Keratin Antibody(AKA),rheumatoid factor(RF) and glucose-6-phosphate isomerase(GPI)in the diagnosis of rheumatoid arthritis(RA).Methods Serum levels of anti-CCP,AKA,RF and GPI were detected in 103 RA patients,91 patients with non-RA autoimmune diseases and 80 healthy controls.Anti-CCP and GPI were detected by ELISA,AKA was detected with indirect immunofluorescence.RF was detected by nephelometric assay.The positive percents of anti-CCP,AKA,RF and GPI were compared in RA patients,non-RA patients and healthy controls.The differences of positive percents of anti-CCP,AKA,RF and GPI in RA patients were compared.The correlations of anti-CCP and RF,GPI were observed.Results The sensitivities of anti-CCP,AKA,RF and GPI were 88.4%,68.0%,90.3% and 84.5% respectively.The specificities were 94.7%,96.5%,71.3% and 71.0% respectively.The positive percent of anti-CCP was obviously higher than AKA in the RA patients(P0.01),and there was an obvious correlation between anti-CCP and RF(P0.01).Conclusion RF has the highest sensitivity for diagnosing RA,while AKA has the highest specificity.Anti-CCP is a good serology index with high sensitivity and specificity for diagnosing RA,so there is great value in the clinical application.
Jiawen Wu - One of the best experts on this subject based on the ideXlab platform.
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complement activation contributes to the anti methicillin resistant staphylococcus aureus effect of natural anti Keratin Antibody
Biochemical and Biophysical Research Communications, 2015Co-Authors: Jingang An, Zhengxiao Li, Yingying Dong, Jiawen WuAbstract:Methicillin-resistant Staphylococcus aureus (MRSA) remains a major public health problem worldwide because of its strong resistance to a variety of antibiotics. Natural immunoglobulin (Ig) M antibodies have been reported to protect against microbial infections. In the present study, the function of a monoclonal natural anti-Keratin Antibody IgM (named 3B4) in MRSA infection was evaluated. The binding of 3B4 to MRSA was studied using immunofluorescence assay and flow cytometry (FCM). The binding of 3B4 to mannose-binding lectin (MBL) and complement activation were detected by ELISA. For the in vivo study, transgenic mice for the VH gene from 3B4 (TgVH 3B4) were used. After infection, the bacterial burden was examined in the kidney, spleen and enterocelia. Inflammatory cytokine levels and the neutrophil ratio in peritoneal lavage fluid (PLF) were assessed by ELISA and FCM, respectively. Additionally, the total serum hemolytic activity (CH50) in the early stage of infection was detected by ELISA. The results showed that 3B4 bound directly to MRSA and MBL, and the interaction between 3B4 and MRSA/MBL led to the activation of the classic and the MBL pathway in vitro. After 48 h of MRSA infection, the bacterial load in the kidney, spleen and enterocelia was significantly decreased in TgVH 3B4 mice (P < 0.05) compared with wild-type mice. Levels of IL-6, TNF-α, and IFN-γ were increased after MRSA infection. The levels of IL-6 and TNF-α in TgVH 3B4 mice were decreased by 49.1% and 59.4% compared to wild-type mice. Additionally, the neutrophil ratio in the PLF of TgVH 3B4 mice was decreased by 65.9%. The CH50 value was significantly higher in TgVH 3B4 mice than in wild-type mice, indicating that 3B4 promoted the activation of the complement system in MRSA infected mice. The results reveal an important role of 3B4 in the anti-MRSA immune response, and the complement activation contributes to this effect.
