The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform
Jun Yamazaki - One of the best experts on this subject based on the ideXlab platform.
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association of caspase 14 and filaggrin expression with Keratinization of the oral mucosa and reconstruction culture rat models
Journal of Periodontal Research, 2014Co-Authors: Hiroshi Murakami, Shigehisa Aoki, Kazuhiko Okamura, Ryuji Sakagami, Jun YamazakiAbstract:Background and Objective Keratinization of the oral mucosa, such as the gingiva, has been shown to be important for periodontal health. Caspase-14 is a protease that plays a role in Keratinization of the epidermis. The objective of this study was to investigate whether the expression of caspase-14 is intimately linked with Keratinization and to examine the effect of the main component of green tea on the improvement of Keratinization in rat oral mucosal preparations. Material and Methods Histological and immunohistochemical analyses and quantitative mRNA measurements of caspase-14 and its substrate filaggrin were performed using different types of rat epithelial tissue and organotypic reconstruction culture models derived from epithelial cells and fibroblasts taken from the rat oral mucosa. Results In the skin, palate, buccal mucosa and esophagus, the degree of Keratinization appeared to be associated with expression of cytokeratin 10. The relative protein and mRNA expression levels of caspase-14 and filaggrin were consistent with the degree of Keratinization in the following order: skin > palate > buccal mucosa > esophagus. The culture models of palatal and buccal mucosa retained a stratified epithelial structure. Expression of caspase-14 appeared to be stronger in the palatal model than in the buccal model. Remarkably, epigallocatechin-3-gallate (EGCG) improved the localization of cytokeratins and increased the expression of caspase-14 and filaggrin. This expression was more intense in the palatal model than in the buccal model, indicating that both models maintain the intrinsic properties of Keratinization of the mucosa from where the cultured cells were derived. Conclusions These results suggest that Keratinization is closely associated with expression of caspase-14 and filaggrin. Our reconstruction models are promising tools for drug evaluation and show that EGCG is beneficial for improving both Keratinization and expression of the linked protease in the oral mucosa.
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Association of caspase‐14 and filaggrin expression with Keratinization of the oral mucosa and reconstruction culture rat models
Journal of Periodontal Research, 2013Co-Authors: Hiroshi Murakami, Shigehisa Aoki, Kazuhiko Okamura, Ryuji Sakagami, Jun YamazakiAbstract:Background and Objective Keratinization of the oral mucosa, such as the gingiva, has been shown to be important for periodontal health. Caspase-14 is a protease that plays a role in Keratinization of the epidermis. The objective of this study was to investigate whether the expression of caspase-14 is intimately linked with Keratinization and to examine the effect of the main component of green tea on the improvement of Keratinization in rat oral mucosal preparations. Material and Methods Histological and immunohistochemical analyses and quantitative mRNA measurements of caspase-14 and its substrate filaggrin were performed using different types of rat epithelial tissue and organotypic reconstruction culture models derived from epithelial cells and fibroblasts taken from the rat oral mucosa. Results In the skin, palate, buccal mucosa and esophagus, the degree of Keratinization appeared to be associated with expression of cytokeratin 10. The relative protein and mRNA expression levels of caspase-14 and filaggrin were consistent with the degree of Keratinization in the following order: skin > palate > buccal mucosa > esophagus. The culture models of palatal and buccal mucosa retained a stratified epithelial structure. Expression of caspase-14 appeared to be stronger in the palatal model than in the buccal model. Remarkably, epigallocatechin-3-gallate (EGCG) improved the localization of cytokeratins and increased the expression of caspase-14 and filaggrin. This expression was more intense in the palatal model than in the buccal model, indicating that both models maintain the intrinsic properties of Keratinization of the mucosa from where the cultured cells were derived. Conclusions These results suggest that Keratinization is closely associated with expression of caspase-14 and filaggrin. Our reconstruction models are promising tools for drug evaluation and show that EGCG is beneficial for improving both Keratinization and expression of the linked protease in the oral mucosa.
Nelly Boehm - One of the best experts on this subject based on the ideXlab platform.
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regulation of differentiation and keratin 10 expression by all trans retinoic acid during the estrous cycle in the rat vaginal epithelium
Cell and Tissue Research, 1996Co-Authors: Danielle Chateau, Nelly BoehmAbstract:In rodents, the vaginal epithelium shows cyclic changes with an alternating pattern of Keratinization under estrogen control and mucification under progesterone control. Retinoids are powerful regulators of cell differentiation, an excess of retinoids suppressing the keratinizing differentiation of keratinocytes. Here, we have examined the vaginal epithelium during the estrous cycle and compare the effects of retinoids on both types of hormonally induced differentiation, i.e. Keratinization and mucification. All-trans retinoic acid was administred either by daily injections during the estrous cycle or by a single injection before the estrogen rise; these two protocols gave similar results. Retinoic acid suppressed estrogen-induced vaginal Keratinization and cytokeratin K10 expression (a biochemical marker of terminal differentiation). Progesterone-induced mucification was not impaired; however, retinoic acid impeded mucous cell desquamation, suggesting an effect of retinoic acid on cell adhesiveness. Retinoic acid induced the appearance of apoptotic-like cells, as revealed by immunocytochemical staining of DNA fragmentation.
Hiroshi Murakami - One of the best experts on this subject based on the ideXlab platform.
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association of caspase 14 and filaggrin expression with Keratinization of the oral mucosa and reconstruction culture rat models
Journal of Periodontal Research, 2014Co-Authors: Hiroshi Murakami, Shigehisa Aoki, Kazuhiko Okamura, Ryuji Sakagami, Jun YamazakiAbstract:Background and Objective Keratinization of the oral mucosa, such as the gingiva, has been shown to be important for periodontal health. Caspase-14 is a protease that plays a role in Keratinization of the epidermis. The objective of this study was to investigate whether the expression of caspase-14 is intimately linked with Keratinization and to examine the effect of the main component of green tea on the improvement of Keratinization in rat oral mucosal preparations. Material and Methods Histological and immunohistochemical analyses and quantitative mRNA measurements of caspase-14 and its substrate filaggrin were performed using different types of rat epithelial tissue and organotypic reconstruction culture models derived from epithelial cells and fibroblasts taken from the rat oral mucosa. Results In the skin, palate, buccal mucosa and esophagus, the degree of Keratinization appeared to be associated with expression of cytokeratin 10. The relative protein and mRNA expression levels of caspase-14 and filaggrin were consistent with the degree of Keratinization in the following order: skin > palate > buccal mucosa > esophagus. The culture models of palatal and buccal mucosa retained a stratified epithelial structure. Expression of caspase-14 appeared to be stronger in the palatal model than in the buccal model. Remarkably, epigallocatechin-3-gallate (EGCG) improved the localization of cytokeratins and increased the expression of caspase-14 and filaggrin. This expression was more intense in the palatal model than in the buccal model, indicating that both models maintain the intrinsic properties of Keratinization of the mucosa from where the cultured cells were derived. Conclusions These results suggest that Keratinization is closely associated with expression of caspase-14 and filaggrin. Our reconstruction models are promising tools for drug evaluation and show that EGCG is beneficial for improving both Keratinization and expression of the linked protease in the oral mucosa.
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Association of caspase‐14 and filaggrin expression with Keratinization of the oral mucosa and reconstruction culture rat models
Journal of Periodontal Research, 2013Co-Authors: Hiroshi Murakami, Shigehisa Aoki, Kazuhiko Okamura, Ryuji Sakagami, Jun YamazakiAbstract:Background and Objective Keratinization of the oral mucosa, such as the gingiva, has been shown to be important for periodontal health. Caspase-14 is a protease that plays a role in Keratinization of the epidermis. The objective of this study was to investigate whether the expression of caspase-14 is intimately linked with Keratinization and to examine the effect of the main component of green tea on the improvement of Keratinization in rat oral mucosal preparations. Material and Methods Histological and immunohistochemical analyses and quantitative mRNA measurements of caspase-14 and its substrate filaggrin were performed using different types of rat epithelial tissue and organotypic reconstruction culture models derived from epithelial cells and fibroblasts taken from the rat oral mucosa. Results In the skin, palate, buccal mucosa and esophagus, the degree of Keratinization appeared to be associated with expression of cytokeratin 10. The relative protein and mRNA expression levels of caspase-14 and filaggrin were consistent with the degree of Keratinization in the following order: skin > palate > buccal mucosa > esophagus. The culture models of palatal and buccal mucosa retained a stratified epithelial structure. Expression of caspase-14 appeared to be stronger in the palatal model than in the buccal model. Remarkably, epigallocatechin-3-gallate (EGCG) improved the localization of cytokeratins and increased the expression of caspase-14 and filaggrin. This expression was more intense in the palatal model than in the buccal model, indicating that both models maintain the intrinsic properties of Keratinization of the mucosa from where the cultured cells were derived. Conclusions These results suggest that Keratinization is closely associated with expression of caspase-14 and filaggrin. Our reconstruction models are promising tools for drug evaluation and show that EGCG is beneficial for improving both Keratinization and expression of the linked protease in the oral mucosa.
Danielle Chateau - One of the best experts on this subject based on the ideXlab platform.
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regulation of differentiation and keratin 10 expression by all trans retinoic acid during the estrous cycle in the rat vaginal epithelium
Cell and Tissue Research, 1996Co-Authors: Danielle Chateau, Nelly BoehmAbstract:In rodents, the vaginal epithelium shows cyclic changes with an alternating pattern of Keratinization under estrogen control and mucification under progesterone control. Retinoids are powerful regulators of cell differentiation, an excess of retinoids suppressing the keratinizing differentiation of keratinocytes. Here, we have examined the vaginal epithelium during the estrous cycle and compare the effects of retinoids on both types of hormonally induced differentiation, i.e. Keratinization and mucification. All-trans retinoic acid was administred either by daily injections during the estrous cycle or by a single injection before the estrogen rise; these two protocols gave similar results. Retinoic acid suppressed estrogen-induced vaginal Keratinization and cytokeratin K10 expression (a biochemical marker of terminal differentiation). Progesterone-induced mucification was not impaired; however, retinoic acid impeded mucous cell desquamation, suggesting an effect of retinoic acid on cell adhesiveness. Retinoic acid induced the appearance of apoptotic-like cells, as revealed by immunocytochemical staining of DNA fragmentation.
Ryuji Sakagami - One of the best experts on this subject based on the ideXlab platform.
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association of caspase 14 and filaggrin expression with Keratinization of the oral mucosa and reconstruction culture rat models
Journal of Periodontal Research, 2014Co-Authors: Hiroshi Murakami, Shigehisa Aoki, Kazuhiko Okamura, Ryuji Sakagami, Jun YamazakiAbstract:Background and Objective Keratinization of the oral mucosa, such as the gingiva, has been shown to be important for periodontal health. Caspase-14 is a protease that plays a role in Keratinization of the epidermis. The objective of this study was to investigate whether the expression of caspase-14 is intimately linked with Keratinization and to examine the effect of the main component of green tea on the improvement of Keratinization in rat oral mucosal preparations. Material and Methods Histological and immunohistochemical analyses and quantitative mRNA measurements of caspase-14 and its substrate filaggrin were performed using different types of rat epithelial tissue and organotypic reconstruction culture models derived from epithelial cells and fibroblasts taken from the rat oral mucosa. Results In the skin, palate, buccal mucosa and esophagus, the degree of Keratinization appeared to be associated with expression of cytokeratin 10. The relative protein and mRNA expression levels of caspase-14 and filaggrin were consistent with the degree of Keratinization in the following order: skin > palate > buccal mucosa > esophagus. The culture models of palatal and buccal mucosa retained a stratified epithelial structure. Expression of caspase-14 appeared to be stronger in the palatal model than in the buccal model. Remarkably, epigallocatechin-3-gallate (EGCG) improved the localization of cytokeratins and increased the expression of caspase-14 and filaggrin. This expression was more intense in the palatal model than in the buccal model, indicating that both models maintain the intrinsic properties of Keratinization of the mucosa from where the cultured cells were derived. Conclusions These results suggest that Keratinization is closely associated with expression of caspase-14 and filaggrin. Our reconstruction models are promising tools for drug evaluation and show that EGCG is beneficial for improving both Keratinization and expression of the linked protease in the oral mucosa.
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Association of caspase‐14 and filaggrin expression with Keratinization of the oral mucosa and reconstruction culture rat models
Journal of Periodontal Research, 2013Co-Authors: Hiroshi Murakami, Shigehisa Aoki, Kazuhiko Okamura, Ryuji Sakagami, Jun YamazakiAbstract:Background and Objective Keratinization of the oral mucosa, such as the gingiva, has been shown to be important for periodontal health. Caspase-14 is a protease that plays a role in Keratinization of the epidermis. The objective of this study was to investigate whether the expression of caspase-14 is intimately linked with Keratinization and to examine the effect of the main component of green tea on the improvement of Keratinization in rat oral mucosal preparations. Material and Methods Histological and immunohistochemical analyses and quantitative mRNA measurements of caspase-14 and its substrate filaggrin were performed using different types of rat epithelial tissue and organotypic reconstruction culture models derived from epithelial cells and fibroblasts taken from the rat oral mucosa. Results In the skin, palate, buccal mucosa and esophagus, the degree of Keratinization appeared to be associated with expression of cytokeratin 10. The relative protein and mRNA expression levels of caspase-14 and filaggrin were consistent with the degree of Keratinization in the following order: skin > palate > buccal mucosa > esophagus. The culture models of palatal and buccal mucosa retained a stratified epithelial structure. Expression of caspase-14 appeared to be stronger in the palatal model than in the buccal model. Remarkably, epigallocatechin-3-gallate (EGCG) improved the localization of cytokeratins and increased the expression of caspase-14 and filaggrin. This expression was more intense in the palatal model than in the buccal model, indicating that both models maintain the intrinsic properties of Keratinization of the mucosa from where the cultured cells were derived. Conclusions These results suggest that Keratinization is closely associated with expression of caspase-14 and filaggrin. Our reconstruction models are promising tools for drug evaluation and show that EGCG is beneficial for improving both Keratinization and expression of the linked protease in the oral mucosa.
