The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform

Jinzhong Zhao - One of the best experts on this subject based on the ideXlab platform.

  • A novel Knitted Scaffold made of microfiber/nanofiber core-sheath yarns for tendon tissue engineering.
    Biomaterials Science, 2020
    Co-Authors: Jiangyu Cai, Xianrui Xie, Liren Wang, Jia Jiang, Jinzhong Zhao
    Abstract:

    Tendon injury is common in sports and other rigorous activities, which may result in dysfunction and disability. Recently, Scaffolds with a Knitted structure have been widely applied for tendon tissue engineering. The purpose of this study was to fabricate a novel Knitted tendon Scaffold made of microfiber/nanofiber core-sheath yarns and evaluate the biocompatibility and the effect of tenogenic differentiation and tendon tissue regeneration in vitro and in vivo. Poly(e-caprolactone) (PCL) microfibers, PCL microfibers-PCL nanofibers (PCL-PCL) and PCL microfiber-silk fibroin/poly(l-lactic acid-co-e-caprolactone) nanofiber (SF/PLCL) core-sheath yarns were fabricated and then Knitted with an automatic knitting machine to produce PCL, PCL-PCL and PCL-SF/PLCL fabric Scaffolds. The characterization of the Scaffolds was performed by using scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy and an universal mechanical instrument. The in vitro experiment showed that rabbit bone marrow stem cells seeded on the Scaffolds exhibited an elongated morphology and proliferated better in the PCL-SF/PLCL group, as compared to the PCL and PCL-PCL groups. Moreover, the PCL-SF/PLCL Scaffold promoted the tenogenic differentiation of the cells for the highest expression levels of the tendon-related genes through down-regulating p-ERK1/2 expression among the three groups. Furthermore, the in vivo study in a rabbit patellar defect model demonstrated that the PCL-SF/PLCL Scaffold could enhance the tissue regeneration and remodeling process as indicated by the better structural and biomechanical properties according to the results of histology, immunohistochemistry, transmission electron microscope examination and biomechanical tests. Therefore, the PCL-SF/PLCL Scaffold is proved to be a promising biomaterial for tendon tissue engineering and a potential candidate for clinical treatment of tendon injury in the future.

  • a novel Knitted Scaffold made of microfiber nanofiber core sheath yarns for tendon tissue engineering
    Biomaterials Science, 2020
    Co-Authors: Jiangyu Cai, Xianrui Xie, Liren Wang, Jia Jiang, Jinzhong Zhao
    Abstract:

    Tendon injury is common in sports and other rigorous activities, which may result in dysfunction and disability. Recently, Scaffolds with a Knitted structure have been widely applied for tendon tissue engineering. The purpose of this study was to fabricate a novel Knitted tendon Scaffold made of microfiber/nanofiber core-sheath yarns and evaluate the biocompatibility and the effect of tenogenic differentiation and tendon tissue regeneration in vitro and in vivo. Poly(e-caprolactone) (PCL) microfibers, PCL microfibers-PCL nanofibers (PCL-PCL) and PCL microfiber-silk fibroin/poly(l-lactic acid-co-e-caprolactone) nanofiber (SF/PLCL) core-sheath yarns were fabricated and then Knitted with an automatic knitting machine to produce PCL, PCL-PCL and PCL-SF/PLCL fabric Scaffolds. The characterization of the Scaffolds was performed by using scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy and an universal mechanical instrument. The in vitro experiment showed that rabbit bone marrow stem cells seeded on the Scaffolds exhibited an elongated morphology and proliferated better in the PCL-SF/PLCL group, as compared to the PCL and PCL-PCL groups. Moreover, the PCL-SF/PLCL Scaffold promoted the tenogenic differentiation of the cells for the highest expression levels of the tendon-related genes through down-regulating p-ERK1/2 expression among the three groups. Furthermore, the in vivo study in a rabbit patellar defect model demonstrated that the PCL-SF/PLCL Scaffold could enhance the tissue regeneration and remodeling process as indicated by the better structural and biomechanical properties according to the results of histology, immunohistochemistry, transmission electron microscope examination and biomechanical tests. Therefore, the PCL-SF/PLCL Scaffold is proved to be a promising biomaterial for tendon tissue engineering and a potential candidate for clinical treatment of tendon injury in the future.

Kyaw Moe - One of the best experts on this subject based on the ideXlab platform.

  • Assembly of bone marrow stromal cell sheets with Knitted poly (L-lactide) Scaffold for engineering ligament analogs.
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Hong Wei Ouyang, Siew Lok Toh, James C.h. Goh, Tong Earn Tay, Kyaw Moe
    Abstract:

    The current cell seeding technique has several disadvantages, such as low efficiency of cell attachment to Scaffolds and the limited strength of cell–gel composite adhesion to Scaffold. These problems warrant further study to improve the assembly of cell to Scaffold. Therefore this study aims to fabricate a bone marrow stromal cells (bMSCs) sheet and assemble it on a Knitted poly (L-lactide) (PLLA) Scaffold for engineering ligament analogs. bMSCs were cultured to form a cell sheet in the presence of ascorbic acid. Once a sheet of bMSCs was obtained, it was assembled onto the Knitted Scaffold by a wrapping technique. Then the assembled structure was held in place in a spinner flask for 4 weeks. The macromorphology, histology, and biomechanics of the grafts were evaluated. The composite of cell sheet/PLLA Scaffold constructs had transformed into tissuelike ligament analogs. Immunohistochemical analysis showed that the components of the analogs were similar to that of ligament tissues, consisting primarily of collagen type I and small amount of collagen type III and tenascin. The failure force of the cell/Scaffold assembly under tension (46.68 ± 2.29 N) was higher than that of the Scaffold group (43.58 ± 2.41 N; p < 0.05), but tensile stiffness of the cell/Scaffold group (20.6 ± 1.417 N/mm) was significantly lower than that of the Scaffold group (27.6 ± 1.449 N/mm; p < 0.05). These data showed that the incorporation of bMSCs sheet onto the PLLA Scaffold could make the analog stronger and more stretchable. Therefore the approach of assembling bMSCs sheet onto Knitted PLLA Scaffold is promising for producing tissuelike and functional ligament analogs under dynamic fluid situation for the purpose of anterior cruciate ligament (ACL) reconstruction. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005

  • Assembly of bone marrow stromal cell sheets with Knitted poly (L-lactide) Scaffold for engineering ligament analogs
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Hong Wei Ouyang, Siew Lok Toh, James C.h. Goh, Tong Earn Tay, Kyaw Moe
    Abstract:

    The current cell seeding technique has several disadvantages, such as low efficiency of cell attachment to Scaffolds and the limited strength of cell-gel composite adhesion to Scaffold. These problems warrant further study to improve the assembly of cell to Scaffold. Therefore this study aims to fabricate a bone marrow stromal cells (bMSCs) sheet and assemble it on a Knitted poly (L-lactide) (PLLA) Scaffold for engineering ligament analogs. bMSCs were cultured to form a cell sheet in the presence of ascorbic acid. Once a sheet of bMSCs was obtained, it was assembled onto the Knitted Scaffold by a wrapping technique. Then the assembled structure was held in place in a spinner flask for 4 weeks. The macromorphology, histology, and biomechanics of the grafts were evaluated. The composite of cell sheet/PLLA Scaffold constructs had transformed into tissuelike ligament analogs. Immuno-histochemical analysis showed that the components of the analogs were similar to that of ligament tissues, consisting primarily of collagen type I and small amount of collagen type III and tenascin. The failure force of the cell/Scaffold assembly under tension (46.68 ′ 2.29 N) was higher than that of the Scaffold group (43.58 ′ 2.41 N; p < 0.05), but tensile stiffness of the cell/Scaffold group (20.6 ′ 1.417 N/mm) was significantly lower than that of the Scaffold group (27.6 ′ 1.449 N/mm;p < 0.05). These data showed that the incorporation of bMSCs sheet onto the PLLA Scaffold could make the analog stronger and more stretchable. Therefore the approach of assembling bMSCs sheet onto Knitted PLLA Scaffold is promising for producing tissuelike and functional ligament analogs under dynamic fluid situation for the purpose of anterior cruciate ligament (ACL) reconstruction.

Hong Wei Ouyang - One of the best experts on this subject based on the ideXlab platform.

  • Assembly of bone marrow stromal cell sheets with Knitted poly (L-lactide) Scaffold for engineering ligament analogs.
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Hong Wei Ouyang, Siew Lok Toh, James C.h. Goh, Tong Earn Tay, Kyaw Moe
    Abstract:

    The current cell seeding technique has several disadvantages, such as low efficiency of cell attachment to Scaffolds and the limited strength of cell–gel composite adhesion to Scaffold. These problems warrant further study to improve the assembly of cell to Scaffold. Therefore this study aims to fabricate a bone marrow stromal cells (bMSCs) sheet and assemble it on a Knitted poly (L-lactide) (PLLA) Scaffold for engineering ligament analogs. bMSCs were cultured to form a cell sheet in the presence of ascorbic acid. Once a sheet of bMSCs was obtained, it was assembled onto the Knitted Scaffold by a wrapping technique. Then the assembled structure was held in place in a spinner flask for 4 weeks. The macromorphology, histology, and biomechanics of the grafts were evaluated. The composite of cell sheet/PLLA Scaffold constructs had transformed into tissuelike ligament analogs. Immunohistochemical analysis showed that the components of the analogs were similar to that of ligament tissues, consisting primarily of collagen type I and small amount of collagen type III and tenascin. The failure force of the cell/Scaffold assembly under tension (46.68 ± 2.29 N) was higher than that of the Scaffold group (43.58 ± 2.41 N; p < 0.05), but tensile stiffness of the cell/Scaffold group (20.6 ± 1.417 N/mm) was significantly lower than that of the Scaffold group (27.6 ± 1.449 N/mm; p < 0.05). These data showed that the incorporation of bMSCs sheet onto the PLLA Scaffold could make the analog stronger and more stretchable. Therefore the approach of assembling bMSCs sheet onto Knitted PLLA Scaffold is promising for producing tissuelike and functional ligament analogs under dynamic fluid situation for the purpose of anterior cruciate ligament (ACL) reconstruction. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005

  • Towards an ideal polymer Scaffold for tendon/ligament tissue engineering
    Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics, 2005
    Co-Authors: Sambit Sahoo, Hong Wei Ouyang, James Cho-hong Goh, T E Tay, Siew Lok Toh
    Abstract:

    Tissueengineering holds promise in treating injured tendons and ligaments byreplacing the injured tissues with "engineered tissues" with identical mechanicaland functional characteristics. A biocompatible, biodegradable, porous Scaffold withoptimized architecture, sufficient surface area for cell attachment, growth andproliferation, faborable mechanical properties, and suitable degradation rate is apre-requisite to achieve success with this aproach. Knitted poly(lactide-co-glycolide)(PLGA) Scaffolds comprising of microfibers of 25 micron diameter werecoated with PLGA nanofibers on their surfaces by electrospinning technique. A cell suspension of pig bone marrow stromal cells(BMSC) was seeded on the Scaffolds by pipetting, and thecell-Scaffold constructs were cultured in a CO2 incubator, at 37°Cfor 1-2 weeks. The "engineered tissues" were then assessedfor cell attachment and proliferation, tissue formation, and mechanical properties. Nanofibers, of diameter 300-900 nm, were spread randomly overthe Knitted Scaffold. The reduction in pore-size from about1 mm (in the Knitted Scaffold) to a few micrometers(in the nano-microScaffold) allowed cell seeding by direct pipetting, andeliminated the need of a cell-delivery system like fibrin gel. BMSCs were seen to attach and proliferate well onthe nano-microScaffold, producing abundant extracellular matrix. Mechanical testing revealedthat the cell-seeded nano-microScaffolds possessed slightly higher values of failureload, elastic-region stiffness and toe-region stiffness, than the unseeded Scaffolds. The combination of superior mechanical strength and integrity ofKnitted microfibers, with the large surface area and improved hydrophilicityof the electrospun nanofibers facilitated cell attachment and new tissueformation. This holds promise in tissue engineering of tendon/ligament. ©2005 COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.

  • Assembly of bone marrow stromal cell sheets with Knitted poly (L-lactide) Scaffold for engineering ligament analogs
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Hong Wei Ouyang, Siew Lok Toh, James C.h. Goh, Tong Earn Tay, Kyaw Moe
    Abstract:

    The current cell seeding technique has several disadvantages, such as low efficiency of cell attachment to Scaffolds and the limited strength of cell-gel composite adhesion to Scaffold. These problems warrant further study to improve the assembly of cell to Scaffold. Therefore this study aims to fabricate a bone marrow stromal cells (bMSCs) sheet and assemble it on a Knitted poly (L-lactide) (PLLA) Scaffold for engineering ligament analogs. bMSCs were cultured to form a cell sheet in the presence of ascorbic acid. Once a sheet of bMSCs was obtained, it was assembled onto the Knitted Scaffold by a wrapping technique. Then the assembled structure was held in place in a spinner flask for 4 weeks. The macromorphology, histology, and biomechanics of the grafts were evaluated. The composite of cell sheet/PLLA Scaffold constructs had transformed into tissuelike ligament analogs. Immuno-histochemical analysis showed that the components of the analogs were similar to that of ligament tissues, consisting primarily of collagen type I and small amount of collagen type III and tenascin. The failure force of the cell/Scaffold assembly under tension (46.68 ′ 2.29 N) was higher than that of the Scaffold group (43.58 ′ 2.41 N; p < 0.05), but tensile stiffness of the cell/Scaffold group (20.6 ′ 1.417 N/mm) was significantly lower than that of the Scaffold group (27.6 ′ 1.449 N/mm;p < 0.05). These data showed that the incorporation of bMSCs sheet onto the PLLA Scaffold could make the analog stronger and more stretchable. Therefore the approach of assembling bMSCs sheet onto Knitted PLLA Scaffold is promising for producing tissuelike and functional ligament analogs under dynamic fluid situation for the purpose of anterior cruciate ligament (ACL) reconstruction.

Siew Lok Toh - One of the best experts on this subject based on the ideXlab platform.

  • Assembly of bone marrow stromal cell sheets with Knitted poly (L-lactide) Scaffold for engineering ligament analogs.
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Hong Wei Ouyang, Siew Lok Toh, James C.h. Goh, Tong Earn Tay, Kyaw Moe
    Abstract:

    The current cell seeding technique has several disadvantages, such as low efficiency of cell attachment to Scaffolds and the limited strength of cell–gel composite adhesion to Scaffold. These problems warrant further study to improve the assembly of cell to Scaffold. Therefore this study aims to fabricate a bone marrow stromal cells (bMSCs) sheet and assemble it on a Knitted poly (L-lactide) (PLLA) Scaffold for engineering ligament analogs. bMSCs were cultured to form a cell sheet in the presence of ascorbic acid. Once a sheet of bMSCs was obtained, it was assembled onto the Knitted Scaffold by a wrapping technique. Then the assembled structure was held in place in a spinner flask for 4 weeks. The macromorphology, histology, and biomechanics of the grafts were evaluated. The composite of cell sheet/PLLA Scaffold constructs had transformed into tissuelike ligament analogs. Immunohistochemical analysis showed that the components of the analogs were similar to that of ligament tissues, consisting primarily of collagen type I and small amount of collagen type III and tenascin. The failure force of the cell/Scaffold assembly under tension (46.68 ± 2.29 N) was higher than that of the Scaffold group (43.58 ± 2.41 N; p < 0.05), but tensile stiffness of the cell/Scaffold group (20.6 ± 1.417 N/mm) was significantly lower than that of the Scaffold group (27.6 ± 1.449 N/mm; p < 0.05). These data showed that the incorporation of bMSCs sheet onto the PLLA Scaffold could make the analog stronger and more stretchable. Therefore the approach of assembling bMSCs sheet onto Knitted PLLA Scaffold is promising for producing tissuelike and functional ligament analogs under dynamic fluid situation for the purpose of anterior cruciate ligament (ACL) reconstruction. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005

  • Towards an ideal polymer Scaffold for tendon/ligament tissue engineering
    Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics, 2005
    Co-Authors: Sambit Sahoo, Hong Wei Ouyang, James Cho-hong Goh, T E Tay, Siew Lok Toh
    Abstract:

    Tissueengineering holds promise in treating injured tendons and ligaments byreplacing the injured tissues with "engineered tissues" with identical mechanicaland functional characteristics. A biocompatible, biodegradable, porous Scaffold withoptimized architecture, sufficient surface area for cell attachment, growth andproliferation, faborable mechanical properties, and suitable degradation rate is apre-requisite to achieve success with this aproach. Knitted poly(lactide-co-glycolide)(PLGA) Scaffolds comprising of microfibers of 25 micron diameter werecoated with PLGA nanofibers on their surfaces by electrospinning technique. A cell suspension of pig bone marrow stromal cells(BMSC) was seeded on the Scaffolds by pipetting, and thecell-Scaffold constructs were cultured in a CO2 incubator, at 37°Cfor 1-2 weeks. The "engineered tissues" were then assessedfor cell attachment and proliferation, tissue formation, and mechanical properties. Nanofibers, of diameter 300-900 nm, were spread randomly overthe Knitted Scaffold. The reduction in pore-size from about1 mm (in the Knitted Scaffold) to a few micrometers(in the nano-microScaffold) allowed cell seeding by direct pipetting, andeliminated the need of a cell-delivery system like fibrin gel. BMSCs were seen to attach and proliferate well onthe nano-microScaffold, producing abundant extracellular matrix. Mechanical testing revealedthat the cell-seeded nano-microScaffolds possessed slightly higher values of failureload, elastic-region stiffness and toe-region stiffness, than the unseeded Scaffolds. The combination of superior mechanical strength and integrity ofKnitted microfibers, with the large surface area and improved hydrophilicityof the electrospun nanofibers facilitated cell attachment and new tissueformation. This holds promise in tissue engineering of tendon/ligament. ©2005 COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.

  • Assembly of bone marrow stromal cell sheets with Knitted poly (L-lactide) Scaffold for engineering ligament analogs
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Hong Wei Ouyang, Siew Lok Toh, James C.h. Goh, Tong Earn Tay, Kyaw Moe
    Abstract:

    The current cell seeding technique has several disadvantages, such as low efficiency of cell attachment to Scaffolds and the limited strength of cell-gel composite adhesion to Scaffold. These problems warrant further study to improve the assembly of cell to Scaffold. Therefore this study aims to fabricate a bone marrow stromal cells (bMSCs) sheet and assemble it on a Knitted poly (L-lactide) (PLLA) Scaffold for engineering ligament analogs. bMSCs were cultured to form a cell sheet in the presence of ascorbic acid. Once a sheet of bMSCs was obtained, it was assembled onto the Knitted Scaffold by a wrapping technique. Then the assembled structure was held in place in a spinner flask for 4 weeks. The macromorphology, histology, and biomechanics of the grafts were evaluated. The composite of cell sheet/PLLA Scaffold constructs had transformed into tissuelike ligament analogs. Immuno-histochemical analysis showed that the components of the analogs were similar to that of ligament tissues, consisting primarily of collagen type I and small amount of collagen type III and tenascin. The failure force of the cell/Scaffold assembly under tension (46.68 ′ 2.29 N) was higher than that of the Scaffold group (43.58 ′ 2.41 N; p < 0.05), but tensile stiffness of the cell/Scaffold group (20.6 ′ 1.417 N/mm) was significantly lower than that of the Scaffold group (27.6 ′ 1.449 N/mm;p < 0.05). These data showed that the incorporation of bMSCs sheet onto the PLLA Scaffold could make the analog stronger and more stretchable. Therefore the approach of assembling bMSCs sheet onto Knitted PLLA Scaffold is promising for producing tissuelike and functional ligament analogs under dynamic fluid situation for the purpose of anterior cruciate ligament (ACL) reconstruction.

James C.h. Goh - One of the best experts on this subject based on the ideXlab platform.

  • Silk-Based Scaffold for Ligament Tissue Engineering
    IFMBE Proceedings, 2008
    Co-Authors: Haifeng Liu, Hongbin Fan, E. J. W. Wong, S. Lok Toh, James C.h. Goh
    Abstract:

    In recent years, silk has been increasingly studied as the Scaffold for ligament tissue engineering due to the biocompatibility, slow degradability, and remarkable mechanical properties. Braided silk Scaffold modified with short polypeptide also significantly increases collagen synthesis on it. To increase cell attachment and tissue infiltration, the braided Scaffold can be incorporated with silk-gelatin microsponges. A novel silk cable-reinforced gelatin/silk fibroin hybrid Scaffold was fabricated, which, apart from providing proper mechanical strength and enlarged surface area, also supported the proliferation and differentiation of MSCs on it. The Knitted silk mesh is another important silk-based Scaffold for its excellent mechanical properties and good nutrients transport. To prevent cells from leaking out of Scaffold after seeding, freeze-dried silk microsponges were incorporated into the macro pores of Knitted Scaffold. In vitro culture demonstrated that MSCs on Scaffolds proliferated vigorously and produced abundant collagen. The transcription levels of ligament-specific genes (collagen I, collagen III, and tenascin-C) also increased significantly with time. The comparison of MSCs and fibroblasts as cell sources for ligament tissue engineering demonstrated that MSC was the most suitable candidate for its vigorous proliferation and ECM production. The MSCs/Knitted Scaffolds were implanted into rabbits to regenerate ACL in vivo. After 24 weeks, histology observation showed that MSCs were distributed throughout the regenerated ligament and exhibited fibroblast morphology. The key ligament ECM components including collagen I, collagen III, and tenascin-C were produced prominently. Furthermore, direct ligament-bone insertion with typical four zones (bone, mineralized fibrocartilage, fibrocartilage, ligament) was reconstructed, which resembled the native structures of ACL-bone insertion. The tensile strength of regenerated ligament also met the mechanical requirements of daily activities. In conclusion, the results imply that silk Scaffold has great potentials in future clinical applications.

  • Assembly of bone marrow stromal cell sheets with Knitted poly (L-lactide) Scaffold for engineering ligament analogs.
    Journal of Biomedical Materials Research Part B, 2005
    Co-Authors: Hong Wei Ouyang, Siew Lok Toh, James C.h. Goh, Tong Earn Tay, Kyaw Moe
    Abstract:

    The current cell seeding technique has several disadvantages, such as low efficiency of cell attachment to Scaffolds and the limited strength of cell–gel composite adhesion to Scaffold. These problems warrant further study to improve the assembly of cell to Scaffold. Therefore this study aims to fabricate a bone marrow stromal cells (bMSCs) sheet and assemble it on a Knitted poly (L-lactide) (PLLA) Scaffold for engineering ligament analogs. bMSCs were cultured to form a cell sheet in the presence of ascorbic acid. Once a sheet of bMSCs was obtained, it was assembled onto the Knitted Scaffold by a wrapping technique. Then the assembled structure was held in place in a spinner flask for 4 weeks. The macromorphology, histology, and biomechanics of the grafts were evaluated. The composite of cell sheet/PLLA Scaffold constructs had transformed into tissuelike ligament analogs. Immunohistochemical analysis showed that the components of the analogs were similar to that of ligament tissues, consisting primarily of collagen type I and small amount of collagen type III and tenascin. The failure force of the cell/Scaffold assembly under tension (46.68 ± 2.29 N) was higher than that of the Scaffold group (43.58 ± 2.41 N; p < 0.05), but tensile stiffness of the cell/Scaffold group (20.6 ± 1.417 N/mm) was significantly lower than that of the Scaffold group (27.6 ± 1.449 N/mm; p < 0.05). These data showed that the incorporation of bMSCs sheet onto the PLLA Scaffold could make the analog stronger and more stretchable. Therefore the approach of assembling bMSCs sheet onto Knitted PLLA Scaffold is promising for producing tissuelike and functional ligament analogs under dynamic fluid situation for the purpose of anterior cruciate ligament (ACL) reconstruction. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005

  • Assembly of bone marrow stromal cell sheets with Knitted poly (L-lactide) Scaffold for engineering ligament analogs
    Journal of Biomedical Materials Research, 2005
    Co-Authors: Hong Wei Ouyang, Siew Lok Toh, James C.h. Goh, Tong Earn Tay, Kyaw Moe
    Abstract:

    The current cell seeding technique has several disadvantages, such as low efficiency of cell attachment to Scaffolds and the limited strength of cell-gel composite adhesion to Scaffold. These problems warrant further study to improve the assembly of cell to Scaffold. Therefore this study aims to fabricate a bone marrow stromal cells (bMSCs) sheet and assemble it on a Knitted poly (L-lactide) (PLLA) Scaffold for engineering ligament analogs. bMSCs were cultured to form a cell sheet in the presence of ascorbic acid. Once a sheet of bMSCs was obtained, it was assembled onto the Knitted Scaffold by a wrapping technique. Then the assembled structure was held in place in a spinner flask for 4 weeks. The macromorphology, histology, and biomechanics of the grafts were evaluated. The composite of cell sheet/PLLA Scaffold constructs had transformed into tissuelike ligament analogs. Immuno-histochemical analysis showed that the components of the analogs were similar to that of ligament tissues, consisting primarily of collagen type I and small amount of collagen type III and tenascin. The failure force of the cell/Scaffold assembly under tension (46.68 ′ 2.29 N) was higher than that of the Scaffold group (43.58 ′ 2.41 N; p < 0.05), but tensile stiffness of the cell/Scaffold group (20.6 ′ 1.417 N/mm) was significantly lower than that of the Scaffold group (27.6 ′ 1.449 N/mm;p < 0.05). These data showed that the incorporation of bMSCs sheet onto the PLLA Scaffold could make the analog stronger and more stretchable. Therefore the approach of assembling bMSCs sheet onto Knitted PLLA Scaffold is promising for producing tissuelike and functional ligament analogs under dynamic fluid situation for the purpose of anterior cruciate ligament (ACL) reconstruction.