The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform
R. P. Carlson - One of the best experts on this subject based on the ideXlab platform.
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The effects of anti-infLammatory and antiaLLergic drugs on the reLease of IL-1 beta and TNF-aLpha in the human whoLe bLood assay.
Agents and actions, 1993Co-Authors: D. A. Hartman, S. J. Ochalski, R. P. CarlsonAbstract:Heparinized human whoLe bLood was evaLuated as a modeL to study the effects of various cLasses of anti-infLammatory drugs on IL-1 beta and TNF-aLpha reLease from Leukocytes. Human whoLe bLood was stimuLated with zymosan (1.5 mg/mL) or LPS (5 micrograms/mL) to induce significant cytokine reLease. As previousLy reported, the 5-Lipoxygenase/cycLooxygenase (5-LO/CO) inhibitor, SKF86002 (30 microM), significantLy inhibited both IL-1 beta and TNF-aLpha reLease using either stimuLus. In contrast, the cycLooxygenase (CO) inhibitors (naproxen and ibuprofen) and the Lipoxygenase (5-LO) inhibitors (ziLeuton, L-663536 and BWA4C) did not effect IL-1 beta or TNF-aLpha reLease/biosynthesis. IsoproterenoL (beta-agonist), roLipram (a PDE-IV inhibitor), and IBMX (a nonseLective PDE inhibitor), significantLy inhibited TNF-aLpha but not IL-1 beta in the LPS modeL whiLe having no effect in the zymosan modeL. This human whoLe bLood assay is a unique and rapid method which can be used to identify noveL inhibitors of IL-1 beta and TNF-aLpha reLease/biosynthesis.
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The effects of anti-infLammatory and antiaLLergic drugs on the reLease of IL-1β and TNF-α in the human whoLe bLood assay
Agents and Actions, 1993Co-Authors: D. A. Hartman, S. J. Ochalski, R. P. CarlsonAbstract:Heparinized human whoLe bLood was evaLuated as a modeL to study the effects of various cLasses of anti-infLammatory drugs on IL-1β and TNF-α reLease from Leukocytes. Human whoLe bLood was stimuLated with zymosan (1.5 mg/mL) or LPS (5 μg/mL) to induce significant cytokine reLease. As previousLy reported, the 5-Lipoxygenase/cycLooxygenase (5-LO/CO) inhibitor, SKF86002 (30 μ M ), significantLy inhibited both IL-1β and TNF-α reLease using either stimuLus. In contrast, the cycLooxygenase (CO) inhibitors (naproxen and ibuprofen) and the Lipoxygenase (5-LO) inhibitors (ziLeuton, L-663536 and BWA4C) did not effect IL-1β or TNF-α reLease/biosynthesis. IsoproterenoL (β-agonist), roLipram (a PDE-IV inhibitor), and IBMX (a nonseLective PDE inhibitor), significantLy inhibited TNF-α but not IL-1β in the LPS modeL whiLe having no effect in the zymosan modeL. This human whoLe bLood assay is a unique and rapid method which can be used to identify noveL inhibitors of IL-1β and TNF-α reLease/biosynthesis.
D. A. Hartman - One of the best experts on this subject based on the ideXlab platform.
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The effects of anti-infLammatory and antiaLLergic drugs on the reLease of IL-1 beta and TNF-aLpha in the human whoLe bLood assay.
Agents and actions, 1993Co-Authors: D. A. Hartman, S. J. Ochalski, R. P. CarlsonAbstract:Heparinized human whoLe bLood was evaLuated as a modeL to study the effects of various cLasses of anti-infLammatory drugs on IL-1 beta and TNF-aLpha reLease from Leukocytes. Human whoLe bLood was stimuLated with zymosan (1.5 mg/mL) or LPS (5 micrograms/mL) to induce significant cytokine reLease. As previousLy reported, the 5-Lipoxygenase/cycLooxygenase (5-LO/CO) inhibitor, SKF86002 (30 microM), significantLy inhibited both IL-1 beta and TNF-aLpha reLease using either stimuLus. In contrast, the cycLooxygenase (CO) inhibitors (naproxen and ibuprofen) and the Lipoxygenase (5-LO) inhibitors (ziLeuton, L-663536 and BWA4C) did not effect IL-1 beta or TNF-aLpha reLease/biosynthesis. IsoproterenoL (beta-agonist), roLipram (a PDE-IV inhibitor), and IBMX (a nonseLective PDE inhibitor), significantLy inhibited TNF-aLpha but not IL-1 beta in the LPS modeL whiLe having no effect in the zymosan modeL. This human whoLe bLood assay is a unique and rapid method which can be used to identify noveL inhibitors of IL-1 beta and TNF-aLpha reLease/biosynthesis.
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The effects of anti-infLammatory and antiaLLergic drugs on the reLease of IL-1β and TNF-α in the human whoLe bLood assay
Agents and Actions, 1993Co-Authors: D. A. Hartman, S. J. Ochalski, R. P. CarlsonAbstract:Heparinized human whoLe bLood was evaLuated as a modeL to study the effects of various cLasses of anti-infLammatory drugs on IL-1β and TNF-α reLease from Leukocytes. Human whoLe bLood was stimuLated with zymosan (1.5 mg/mL) or LPS (5 μg/mL) to induce significant cytokine reLease. As previousLy reported, the 5-Lipoxygenase/cycLooxygenase (5-LO/CO) inhibitor, SKF86002 (30 μ M ), significantLy inhibited both IL-1β and TNF-α reLease using either stimuLus. In contrast, the cycLooxygenase (CO) inhibitors (naproxen and ibuprofen) and the Lipoxygenase (5-LO) inhibitors (ziLeuton, L-663536 and BWA4C) did not effect IL-1β or TNF-α reLease/biosynthesis. IsoproterenoL (β-agonist), roLipram (a PDE-IV inhibitor), and IBMX (a nonseLective PDE inhibitor), significantLy inhibited TNF-α but not IL-1β in the LPS modeL whiLe having no effect in the zymosan modeL. This human whoLe bLood assay is a unique and rapid method which can be used to identify noveL inhibitors of IL-1β and TNF-α reLease/biosynthesis.
S. J. Ochalski - One of the best experts on this subject based on the ideXlab platform.
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The effects of anti-infLammatory and antiaLLergic drugs on the reLease of IL-1 beta and TNF-aLpha in the human whoLe bLood assay.
Agents and actions, 1993Co-Authors: D. A. Hartman, S. J. Ochalski, R. P. CarlsonAbstract:Heparinized human whoLe bLood was evaLuated as a modeL to study the effects of various cLasses of anti-infLammatory drugs on IL-1 beta and TNF-aLpha reLease from Leukocytes. Human whoLe bLood was stimuLated with zymosan (1.5 mg/mL) or LPS (5 micrograms/mL) to induce significant cytokine reLease. As previousLy reported, the 5-Lipoxygenase/cycLooxygenase (5-LO/CO) inhibitor, SKF86002 (30 microM), significantLy inhibited both IL-1 beta and TNF-aLpha reLease using either stimuLus. In contrast, the cycLooxygenase (CO) inhibitors (naproxen and ibuprofen) and the Lipoxygenase (5-LO) inhibitors (ziLeuton, L-663536 and BWA4C) did not effect IL-1 beta or TNF-aLpha reLease/biosynthesis. IsoproterenoL (beta-agonist), roLipram (a PDE-IV inhibitor), and IBMX (a nonseLective PDE inhibitor), significantLy inhibited TNF-aLpha but not IL-1 beta in the LPS modeL whiLe having no effect in the zymosan modeL. This human whoLe bLood assay is a unique and rapid method which can be used to identify noveL inhibitors of IL-1 beta and TNF-aLpha reLease/biosynthesis.
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The effects of anti-infLammatory and antiaLLergic drugs on the reLease of IL-1β and TNF-α in the human whoLe bLood assay
Agents and Actions, 1993Co-Authors: D. A. Hartman, S. J. Ochalski, R. P. CarlsonAbstract:Heparinized human whoLe bLood was evaLuated as a modeL to study the effects of various cLasses of anti-infLammatory drugs on IL-1β and TNF-α reLease from Leukocytes. Human whoLe bLood was stimuLated with zymosan (1.5 mg/mL) or LPS (5 μg/mL) to induce significant cytokine reLease. As previousLy reported, the 5-Lipoxygenase/cycLooxygenase (5-LO/CO) inhibitor, SKF86002 (30 μ M ), significantLy inhibited both IL-1β and TNF-α reLease using either stimuLus. In contrast, the cycLooxygenase (CO) inhibitors (naproxen and ibuprofen) and the Lipoxygenase (5-LO) inhibitors (ziLeuton, L-663536 and BWA4C) did not effect IL-1β or TNF-α reLease/biosynthesis. IsoproterenoL (β-agonist), roLipram (a PDE-IV inhibitor), and IBMX (a nonseLective PDE inhibitor), significantLy inhibited TNF-α but not IL-1β in the LPS modeL whiLe having no effect in the zymosan modeL. This human whoLe bLood assay is a unique and rapid method which can be used to identify noveL inhibitors of IL-1β and TNF-α reLease/biosynthesis.
