The Experts below are selected from a list of 155526 Experts worldwide ranked by ideXlab platform
Christian Franck - One of the best experts on this subject based on the ideXlab platform.
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high resolution Large Deformation 3d traction force microscopy
Biophysical Journal, 2015Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction force microscopy (TFM) is a powerful approach of quantifying cell-material interactions, which over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions.This talk presents a new high-resolution 3D TFM algorithm, which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate traction forces. Based on our previous 3D TFM technique we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
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high resolution Large Deformation 3d traction force microscopy
PLOS ONE, 2014Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction Force Microscopy (TFM) is a powerful approach for quantifying cell-material interactions that over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality, almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions. Here we present a new high resolution 3D TFM algorithm which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate the traction forces. Based on our previous 3D TFM technique, we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
Jonathan S. Reichner - One of the best experts on this subject based on the ideXlab platform.
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high resolution Large Deformation 3d traction force microscopy
Biophysical Journal, 2015Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction force microscopy (TFM) is a powerful approach of quantifying cell-material interactions, which over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions.This talk presents a new high-resolution 3D TFM algorithm, which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate traction forces. Based on our previous 3D TFM technique we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
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high resolution Large Deformation 3d traction force microscopy
PLOS ONE, 2014Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction Force Microscopy (TFM) is a powerful approach for quantifying cell-material interactions that over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality, almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions. Here we present a new high resolution 3D TFM algorithm which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate the traction forces. Based on our previous 3D TFM technique, we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
Jennet Toyjanova - One of the best experts on this subject based on the ideXlab platform.
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high resolution Large Deformation 3d traction force microscopy
Biophysical Journal, 2015Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction force microscopy (TFM) is a powerful approach of quantifying cell-material interactions, which over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions.This talk presents a new high-resolution 3D TFM algorithm, which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate traction forces. Based on our previous 3D TFM technique we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
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high resolution Large Deformation 3d traction force microscopy
PLOS ONE, 2014Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction Force Microscopy (TFM) is a powerful approach for quantifying cell-material interactions that over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality, almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions. Here we present a new high resolution 3D TFM algorithm which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate the traction forces. Based on our previous 3D TFM technique, we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
Diane Hoffmankim - One of the best experts on this subject based on the ideXlab platform.
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high resolution Large Deformation 3d traction force microscopy
Biophysical Journal, 2015Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction force microscopy (TFM) is a powerful approach of quantifying cell-material interactions, which over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions.This talk presents a new high-resolution 3D TFM algorithm, which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate traction forces. Based on our previous 3D TFM technique we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
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high resolution Large Deformation 3d traction force microscopy
PLOS ONE, 2014Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction Force Microscopy (TFM) is a powerful approach for quantifying cell-material interactions that over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality, almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions. Here we present a new high resolution 3D TFM algorithm which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate the traction forces. Based on our previous 3D TFM technique, we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
Eyal Barkochba - One of the best experts on this subject based on the ideXlab platform.
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high resolution Large Deformation 3d traction force microscopy
Biophysical Journal, 2015Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction force microscopy (TFM) is a powerful approach of quantifying cell-material interactions, which over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions.This talk presents a new high-resolution 3D TFM algorithm, which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate traction forces. Based on our previous 3D TFM technique we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
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high resolution Large Deformation 3d traction force microscopy
PLOS ONE, 2014Co-Authors: Jennet Toyjanova, Eyal Barkochba, Cristina Lopezfagundo, Diane Hoffmankim, Jonathan S. Reichner, Christian FranckAbstract:Traction Force Microscopy (TFM) is a powerful approach for quantifying cell-material interactions that over the last two decades has contributed significantly to our understanding of cellular mechanosensing and mechanotransduction. In addition, recent advances in three-dimensional (3D) imaging and traction force analysis (3D TFM) have highlighted the significance of the third dimension in influencing various cellular processes. Yet irrespective of dimensionality, almost all TFM approaches have relied on a linear elastic theory framework to calculate cell surface tractions. Here we present a new high resolution 3D TFM algorithm which utilizes a Large Deformation formulation to quantify cellular displacement fields with unprecedented resolution. The results feature some of the first experimental evidence that cells are indeed capable of exerting Large material Deformations, which require the formulation of a new theoretical TFM framework to accurately calculate the traction forces. Based on our previous 3D TFM technique, we reformulate our approach to accurately account for Large material Deformation and quantitatively contrast and compare both linear and Large Deformation frameworks as a function of the applied cell Deformation. Particular attention is paid in estimating the accuracy penalty associated with utilizing a traditional linear elastic approach in the presence of Large Deformation gradients.
