The Experts below are selected from a list of 246 Experts worldwide ranked by ideXlab platform
Bo Dong - One of the best experts on this subject based on the ideXlab platform.
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identification of differentially expressed proteins involved in the early Larval Development of the pacific oyster crassostrea gigas
Journal of Proteomics, 2012Co-Authors: Pin Huan, Hongxia Wang, Bo DongAbstract:The trochophore and D-veliger are two pivotal but distinct Developmental stages in bivalve Larval Development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early Larval Development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk Larval Development. Bivalve Larval Development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks. (c) 2012 Elsevier B.V. All rights reserved.
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Identification of differentially expressed proteins involved in the early Larval Development of the Pacific oyster Crassostrea gigas
Journal of Proteomics, 2012Co-Authors: Pin Huan, Hongxia Wang, Bo Dong, Baozhong LiuAbstract:The trochophore and D-veliger are two pivotal but distinct Developmental stages in bivalve Larval Development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early Larval Development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk Larval Development. Bivalve Larval Development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks. © 2012 Elsevier B.V.
Pin Huan - One of the best experts on this subject based on the ideXlab platform.
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identification of differentially expressed proteins involved in the early Larval Development of the pacific oyster crassostrea gigas
Journal of Proteomics, 2012Co-Authors: Pin Huan, Hongxia Wang, Bo DongAbstract:The trochophore and D-veliger are two pivotal but distinct Developmental stages in bivalve Larval Development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early Larval Development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk Larval Development. Bivalve Larval Development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks. (c) 2012 Elsevier B.V. All rights reserved.
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Identification of differentially expressed proteins involved in the early Larval Development of the Pacific oyster Crassostrea gigas
Journal of Proteomics, 2012Co-Authors: Pin Huan, Hongxia Wang, Bo Dong, Baozhong LiuAbstract:The trochophore and D-veliger are two pivotal but distinct Developmental stages in bivalve Larval Development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early Larval Development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk Larval Development. Bivalve Larval Development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks. © 2012 Elsevier B.V.
Baozhong Liu - One of the best experts on this subject based on the ideXlab platform.
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Identification of differentially expressed proteins involved in the early Larval Development of the Pacific oyster Crassostrea gigas
Journal of Proteomics, 2012Co-Authors: Pin Huan, Hongxia Wang, Bo Dong, Baozhong LiuAbstract:The trochophore and D-veliger are two pivotal but distinct Developmental stages in bivalve Larval Development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early Larval Development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk Larval Development. Bivalve Larval Development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks. © 2012 Elsevier B.V.
Hongxia Wang - One of the best experts on this subject based on the ideXlab platform.
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identification of differentially expressed proteins involved in the early Larval Development of the pacific oyster crassostrea gigas
Journal of Proteomics, 2012Co-Authors: Pin Huan, Hongxia Wang, Bo DongAbstract:The trochophore and D-veliger are two pivotal but distinct Developmental stages in bivalve Larval Development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early Larval Development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk Larval Development. Bivalve Larval Development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks. (c) 2012 Elsevier B.V. All rights reserved.
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Identification of differentially expressed proteins involved in the early Larval Development of the Pacific oyster Crassostrea gigas
Journal of Proteomics, 2012Co-Authors: Pin Huan, Hongxia Wang, Bo Dong, Baozhong LiuAbstract:The trochophore and D-veliger are two pivotal but distinct Developmental stages in bivalve Larval Development. Complex morphological and physiological processes are involved in the transition between these stages, yet the underlying molecular mechanisms remain largely elusive. In this study, a comparative proteomic analysis was conducted to identify the proteins related to early Larval Development of the Pacific oyster Crassostrea gigas. Fifty proteins showing different levels of expression were screened. These spots were further analyzed using LC-MS/MS and twenty of them were identified. Together with information from Western blotting and real-time PCR assay of some annotated genes, our results provide insight into novel aspects of cellular processes and protein function in oyster larvae. For example, it was shown that cell division was unexpectedly slowed down in D-veliger larvae, while substantial gene transcription and biochemical activity were revealed. "Quality control" proteins were identified for the first time to be involved in mollusk Larval Development. Bivalve Larval Development provides an elegant system for studying cellular processes and tissue morphogenesis. Our data shed light on the molecular mechanisms underlying these complex processes and regulatory networks. © 2012 Elsevier B.V.
Rui Miao - One of the best experts on this subject based on the ideXlab platform.
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an ecm to nucleus signaling pathway activates lysosomes for c elegans Larval Development
Developmental Cell, 2020Co-Authors: Rui Miao, Meijiao Li, Qianqian Zhang, Chonglin Yang, Xiaochen WangAbstract:Summary Lysosomes degrade macromolecular cargos, recycle catabolites, and serve as signaling platforms to maintain cell homeostasis, but their role at the tissue level is unclear. Here, we investigate lysosome regulation and function during C. elegans molting, a specialized extracellular matrix (ECM) remodeling process essential for Larval Development. We found that lysosomes are specifically activated in the epidermis at molt when the apical ECM (cuticle) is being replaced. Impaired lysosome function affects endocytic cargo degradation, suppresses elevated protein synthesis at molt, and causes molting defects. Disturbance of ECM-epidermis attachments triggers lysosomal activation and induces expression of the vacuolar H+-ATPase (V-ATPase), which is mediated by the GATA transcription factor ELT-3 and the STAT family protein STA-2. Our study reveals an ECM-to-nucleus signaling pathway that activates lysosomes to facilitate ECM remodeling essential for Larval Development.
