The Experts below are selected from a list of 8970 Experts worldwide ranked by ideXlab platform
Yalong Bai - One of the best experts on this subject based on the ideXlab platform.
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A sensitive Lateral Flow Test strip based on silica nanoparticle/CdTe quantum dot composite reporter probes
RSC Advances, 2012Co-Authors: Yalong Bai, Chunyuan Tian, Xinlin Wei, Yuanfeng Wang, Dapeng Wang, Xianming ShiAbstract:Novel silica nanoparticle/CdTe quantum dot (QD) composite probes were prepared and used as reporters in fluorescent Lateral Flow Test strips. When α-fetoprotein (AFP) was used as a model analyte, the results indicated that the fluorescent Test strips were at least 10 times more sensitive than conventional gold-based Test strips.
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a sensitive Lateral Flow Test strip based on silica nanoparticle cdte quantum dot composite reporter probes
RSC Advances, 2012Co-Authors: Yalong Bai, Chunyuan Tian, Xinlin Wei, Yuanfeng Wang, Dapeng Wang, Xianming ShiAbstract:Novel silica nanoparticle/CdTe quantum dot (QD) composite probes were prepared and used as reporters in fluorescent Lateral Flow Test strips. When α-fetoprotein (AFP) was used as a model analyte, the results indicated that the fluorescent Test strips were at least 10 times more sensitive than conventional gold-based Test strips.
Xianming Shi - One of the best experts on this subject based on the ideXlab platform.
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A sensitive Lateral Flow Test strip based on silica nanoparticle/CdTe quantum dot composite reporter probes
RSC Advances, 2012Co-Authors: Yalong Bai, Chunyuan Tian, Xinlin Wei, Yuanfeng Wang, Dapeng Wang, Xianming ShiAbstract:Novel silica nanoparticle/CdTe quantum dot (QD) composite probes were prepared and used as reporters in fluorescent Lateral Flow Test strips. When α-fetoprotein (AFP) was used as a model analyte, the results indicated that the fluorescent Test strips were at least 10 times more sensitive than conventional gold-based Test strips.
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a sensitive Lateral Flow Test strip based on silica nanoparticle cdte quantum dot composite reporter probes
RSC Advances, 2012Co-Authors: Yalong Bai, Chunyuan Tian, Xinlin Wei, Yuanfeng Wang, Dapeng Wang, Xianming ShiAbstract:Novel silica nanoparticle/CdTe quantum dot (QD) composite probes were prepared and used as reporters in fluorescent Lateral Flow Test strips. When α-fetoprotein (AFP) was used as a model analyte, the results indicated that the fluorescent Test strips were at least 10 times more sensitive than conventional gold-based Test strips.
Byunggee Kim - One of the best experts on this subject based on the ideXlab platform.
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diagnostic ability of salivary matrix metalloproteinase 9 Lateral Flow Test point of care Test for periodontitis
Journal of Clinical Periodontology, 2020Co-Authors: Hyun-duck Kim, Chang-soo Lee, Hyun-jae Cho, Sumin Jeon, Youngnim Choi, Sungtae Kim, Danhee Kim, Hyun Jin Lee, Heejin Jeong, Byunggee KimAbstract:AIM This cross-sectional study aimed to examine the diagnostic ability of salivary matrix metalloproteinase (MMP)-9 Lateral Flow Test (LFT) point-of-care (POC) kit and develop an algorithm for diagnosis of periodontitis. MATERIALS AND METHODS Through Seoul National Dental Hospital, 137 participants (46 LFT negatives, 91 LFT positives) were recruited. For salivary diagnostics, 150 μl of the unstimulated saliva was applied to LFT-POC kit. To make a diagnosis of periodontitis, stage II-IV in modified new international classification system was used. Covariates encompassing age, sex, smoking and obesity were evaluated through face-to-face interview. Enzyme-linked immunosorbent assay was used for quantification of salivary MMP-9. To develop a diagnostic algorithm, multivariable logistic regression analysis was used. Receiver operating characteristic curve was applied for evaluating diagnostic ability. RESULTS Diagnostic ability of salivary MMP-9 LFT-POC Test was 0.82 (sensitivity of 0.92, specificity of 0.72) in total participants. Diagnostic algorithm using POC Test resulted in a response equation, that is algorithm score = -3.675 + 2.877*LFT + 0.034*age + 0.121*sex + 0.372*smoking + 0.192*obesity. Diagnostic ability of the algorithm was 0.88 (sensitivity of 0.92, specificity of 0.85) with cut-off score of 0.589. CONCLUSIONS Salivary MMP-9 LFT-POC kit showed appropriate diagnostic ability for periodontitis and would be an efficient tool for screening of periodontitis.
Boris B Dzantiev - One of the best experts on this subject based on the ideXlab platform.
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Highly sensitive Lateral Flow Test with indirect labelling for zearalenone in baby food
Food and Agricultural Immunology, 2020Co-Authors: Anastasiya V. Bartosh, Anatoly V Zherdev, Hua Kuang, Alina V. Petrakova, Alexandr Е. Urusov, Boris B DzantievAbstract:Maximum permissible levels of mycotoxins in baby food may be 1% of those in ordinary food. Therefore, highly sensitive methods of mycotoxin control are in demand. To detect such low amounts, expens...
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Gold nanoparticles of different shape for bicolor Lateral Flow Test
Analytical Biochemistry, 2018Co-Authors: Alina V. Petrakova, Anatoly V Zherdev, Alexandr E. Urusov, Boris B DzantievAbstract:Abstract Spherical gold nanoparticles are the most commonly used marker in Lateral Flow assays. However, the widespread practice of using identical coloration for the Test and control zones of Test strips can lead to erroneous interpretations of the assay's results. We propose an immunochromatographic Test strip with lines of different colors. For this purpose, gold nanoparticles of different shapes were used, namely blue nanoFlowers in the Test zone and red gold nanospheres in the control zone. A detailed synthesis procedure for nanoparticles and their conjugates is considered and design parameters for optimal results are described. For the first time, nanoparticles of different shapes have been combined in the Test strip with indirect labeling of specific antibodies (via their interaction with labeled secondary antibodies). Using the T-2 toxin (T2T) as an example, an instrumental detection limit of 30 pg/ml and a working range 0.06–0.9 ng/mL were achieved in an analysis of water-organic corn extracts.
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quantum dot based Lateral Flow immunoassay for detection of chloramphenicol in milk
Analytical and Bioanalytical Chemistry, 2013Co-Authors: Anna N Berlina, Nadezhda A Taranova, Yuri Yu Vengerov, Anatoly V Zherdev, Boris B DzantievAbstract:A novel rapid (20 min) fluorescent Lateral Flow Test for chloramphenicol (CAP) detection in milk was developed. The chosen format is a binding-inhibition assay. Water-soluble quantum dots with an emission peak at 625 nm were applied as a label. Milk samples were diluted by 20 % with phosphate buffer to eliminate the matrix effect. The result of the assay could be seen by eye under UV light excitation or registered by a portable power-dependent photometer. The limit of CAP detection by the second approach is 0.2 ng/mL, and the limit of quantitation is 0.3 ng/mL.
Chunyuan Tian - One of the best experts on this subject based on the ideXlab platform.
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A sensitive Lateral Flow Test strip based on silica nanoparticle/CdTe quantum dot composite reporter probes
RSC Advances, 2012Co-Authors: Yalong Bai, Chunyuan Tian, Xinlin Wei, Yuanfeng Wang, Dapeng Wang, Xianming ShiAbstract:Novel silica nanoparticle/CdTe quantum dot (QD) composite probes were prepared and used as reporters in fluorescent Lateral Flow Test strips. When α-fetoprotein (AFP) was used as a model analyte, the results indicated that the fluorescent Test strips were at least 10 times more sensitive than conventional gold-based Test strips.
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a sensitive Lateral Flow Test strip based on silica nanoparticle cdte quantum dot composite reporter probes
RSC Advances, 2012Co-Authors: Yalong Bai, Chunyuan Tian, Xinlin Wei, Yuanfeng Wang, Dapeng Wang, Xianming ShiAbstract:Novel silica nanoparticle/CdTe quantum dot (QD) composite probes were prepared and used as reporters in fluorescent Lateral Flow Test strips. When α-fetoprotein (AFP) was used as a model analyte, the results indicated that the fluorescent Test strips were at least 10 times more sensitive than conventional gold-based Test strips.