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Allah Dad Talpur - One of the best experts on this subject based on the ideXlab platform.
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mentha piperita peppermint as feed additive enhanced growth performance survival immune response and disease resistance of asian seabass Lates Calcarifer bloch against vibrio harveyi infection
Aquaculture, 2014Co-Authors: Allah Dad TalpurAbstract:Abstract This study was aimed to assess the potential effects of Mentha piperita on the growth performance, immune response and protection against Vibrio harveyi infection in Asian sea bass Lates Calcarifer. A control diet without and a series of test diets incorporating M. piperita-diet (MP diet) at 1, 2, 3, 4 and 5 g/kg were prepared and fed to the fish daily for a four-week period. The results showed that MP diet in feed led to reduced mortalities and significantly improved survival, weight gain and feed conversion ratio for treated groups over the control. Dietary M. piperita significantly increased erythrocytes, leucocytes, haematocrit, haemoglobin, phagocytic activity, respiratory burst, lysozyme, anti-protease and bactericidal activities in treated fish. Significantly higher (p
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nutritional effects of ginger zingiber officinale roscoe on immune response of asian sea bass Lates Calcarifer bloch and disease resistance against vibrio harveyi
Aquaculture, 2013Co-Authors: Allah Dad Talpur, Mhd Ikhwanuddin, Abolmunafi Ambok BolongAbstract:Abstract The present study was aimed at determining the effects of ginger (Zingiber officinale Roscoe) as feed additive on Asian sea bass, Lates Calcarifer culture. Experimental diets containing ginger 1, 2, 3, 5 and 10 g/kg of feed were fed to L. Calcarifer and control was fed with no ginger. After the feeding trial for 15 days, fish were challenged with Vibrio harveyi and mortality was recorded for two weeks. Ginger diet led to control of experimental infection in L. Calcarifer. Highest survival (86.6%) was achieved in groups fed with ginger at 5 and 10 g/kg feed respectively, compared to the control (26.7%). In addition, there was a significant increase in weight gain, growth and feed conversion in those fish fed ginger diet. Ginger diet influenced the haematological parameters, biochemical indices and immunological activities. The number of erythrocytes (RBC) and leucocytes (WBC) was significantly (p
Muhammad A. B. Siddik - One of the best experts on this subject based on the ideXlab platform.
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influence of fish protein hydrolysate produced from industrial residues on antioxidant activity cytokine expression and gut microbial communities in juvenile barramundi Lates Calcarifer
Fish & Shellfish Immunology, 2020Co-Authors: Muhammad A. B. Siddik, Janet Howieson, Ravi Fotedar, Reaz Chaklader, Javed Foysal, Sanjay GuptaAbstract:Abstract The present study investigated the supplemental effects of tuna hydrolysate (TH) in poultry by-product meal (PBM) and dietary fishmeal (FM) diets on antioxidant enzymatic activities, gut microbial communities and expression of cytokine genes in the distal intestine of juvenile barramundi, Lates Calcarifer. Fish were fed with fermented (FPBM + TH) as well as non-fermented PBM (PBM + TH) and FM (FMBD + TH) diets with 10% TH supplementation for 10 weeks. A basal diet prepared without TH supplementation served as control. The results showed that the activity of glutathione peroxidase was significantly higher in FPBM + TH than the control, while the malondialdehyde and catalase activities were unchanged. FPBM + TH diet significantly (P
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Growth and health of juvenile barramundi (Lates Calcarifer) challenged with DO hypoxia after feeding various inclusions of germinated, fermented and untreated peanut meals.
'Public Library of Science (PLoS)', 2020Co-Authors: Binh Van Vo, Muhammad A. B. Siddik, Ravi Fotedar, Md Reaz Chaklader, Ashfaqun Nahar, Md Javed Foysal, Dien Phan Bui, Huy Quang NguyenAbstract:Peanut (Arachis hypogaea) is mainly grown for oil extraction and the remaining oil-free seed referred as peanut meal (PM) leaves with high protein content which can be a possible substitute for fishmeal in aqua-diets. This study evaluates the suitability of three types of processed peanut seeds, namely untreated PM (UPM), fermented PM (FPM), and germinated PM (GPM) from peanut seeds to replace fishmeal in barramundi (Lates Calcarifer) diets cultured under a commercial production environment. Nine formulated diets having 3 inclusion levels from the 3 different peanuts (15%, 30% and 60% fishmeal replacement) were evaluated against a control without PM. The performance of various types and levels of PMs was assessed by examining the growth, gut and liver condition and survival of fish after eight weeks of feeding the test diets. The immunological responses of juvenile barramundi were assessed by exposing the fish to the hypoxic conditions for 4 hours. The results showed that fermentation and germination significantly (P
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beneficial effects of tuna hydrolysate in poultry by product meal diets on growth immune response intestinal health and disease resistance to vibrio harveyi in juvenile barramundi Lates Calcarifer
Fish & Shellfish Immunology, 2019Co-Authors: Muhammad A. B. Siddik, Janet Howieson, Ravi FotedarAbstract:Abstract This study was conducted to investigate the effects that tuna hydrolysate (TH) supplementation in poultry by-product meal (PBM) diets would have on growth, immunity and resistance to Vibrio harveyi infection in juvenile barramundi, Lates Calcarifer. Five isonitrogenous and isocaloric diets containing fishmeal (FM) without TH supplementation (control) and four diets with 10% TH supplementation viz. a FM protein diet (FMBD + TH), a 75% PBM protein diet (LPBM + TH) and two 90% PBM protein diets, either bioprocessed (BPBM + TH) or unprocessed (HPBM + TH), were formulated for juvenile barramundi, Lates Calcarifer. The diets were fed to triplicate groups of juvenile barramundi (average pool weight 12.63 ± 0.11 g) for 10 weeks. Significantly (P
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Dietary tuna hydrolysate moduLates growth performance, immune response, intestinal morphology and resistance to Streptococcus iniae in juvenile barramundi, Lates Calcarifer
Scientific Reports, 2018Co-Authors: Muhammad A. B. Siddik, Janet Howieson, Gavin J. Partridge, Ravi Fotedar, Hosna GholipourkananiAbstract:This study investigated the effects of tuna hydrolysate (TH) inclusion in fishmeal (FM) based diets on the growth performance, innate immune response, intestinal health and resistance to Streptococcus iniae infection in juvenile barramundi, Lates Calcarifer . Five isonitrogenous and isoenergetic experimental diets were prepared with TH, replacing FM at levels of 0% (control) 5%, 10%, 15% and 20%, and fed fish to apparent satiation three times daily for 8 weeks. The results showed that fish fed diets containing 5% and 10% TH had significantly higher final body weight and specific growth rate than the control. A significant reduction in blood glucose was found in fish fed 10%, 15% and 20% TH compared to those in the control whereas none of the other measured blood and serum indices were influenced by TH inclusion. Histological observation revealed a significant enhancement in goblet cell numbers in distal intestine of fish fed 5 to 10% TH in the diet. Moreover, fish fed 10% TH exhibited the highest resistance against Streptococcus iniae infection during a bacterial challenge trial. These findings therefore demonstrate that the replacement of 5 to 10% FM with TH improves growth, immune response, intestinal health and disease resistance in juvenile barramundi.
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Growth, biochemical response and liver health of juvenile barramundi (Lates Calcarifer) fed fermented and non-fermented tuna hydrolysate as fishmeal protein replacement ingredients
PeerJ Inc., 2018Co-Authors: Muhammad A. B. Siddik, Janet Howieson, Ilham Ilham, Ravi FotedarAbstract:Conventional aquaculture feed materials available in Australia are expensive, which has prompted the search for alternatives that would be cost-effective and locally available. The present study was undertaken in order to maximize the use of a tuna hydrolysate (TH), which was produced locally from the tuna-processing discards. The growth performance, biochemical status, antioxidant capacity and liver health of juvenile barramundi (Lates Calcarifer) were assessed. Two series of isonitrogenous and isocaloric diets labelled as TH50, TH75 (non-fermented tuna hydrolysate) and FTH50, FTH75 (fermented tuna hydrolysate) were formulated to replace FM at 50% and 75%, respectively. A basal diet without the TH supplementation was used as a control. The experimental diets were fed to the triplicate groups of fish three times a day for 56 days. The results of the experiment revealed that fish fed on both fermented and non-fermented TH-containing diets significantly reduced (p
Jinyong Zhang - One of the best experts on this subject based on the ideXlab platform.
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outbreak of trypanosomiasis in net cage cultured barramundi Lates Calcarifer perciformes latidae associated with trypanosoma epinepheli kinetoplastida in south china sea
Aquaculture, 2019Co-Authors: Dan Luo, X H Liu, Hiroshi Sato, Jinyong ZhangAbstract:Abstract The barramundi or Asian Sea bass (Lates Calcarifer) is of high economic importance for aquaculture worldwide. The present work report a trypanosomiosis of net cage reared barramundi in South China Sea and identified Trypanosoma epinepheli to be the causative agent, which expand the host range of this blood flagellate. This epizootic trypanosomiosis was of distinctly seasonal pattern which occurred from middle-to-late March to middle-to-late May, when water temperature ranged from 21 to 28°C and salinity from 30-33ppt.The infected fish showed typical clinical symptoms of fish trypanosomiosis, including loss of appetite, lethargy, slight emaciation, slightly darken body, swimming lonely and restlessly at the water surface. Mild anemia in gills and remarkable splenomegaly were observed after necropsy. Histopathological investigations showed distinct pathological changes in gill, brain, liver, spleen and kidney, almost consistent with the previous report. This study is the first report of trypanosomiasis in this increasingly important mariculture fish in China.
Bhernama, Bhayu Gita - One of the best experts on this subject based on the ideXlab platform.
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EKSTRAKSI GELATIN DARI TULANG IKAN KAKAP PUTIH (Lates Calcarifer) DENGAN ASAM HCl
'Universitas Nusa Bangsa', 2020Co-Authors: Bhernama, Bhayu GitaAbstract:Extraction of Gelatin from Fish White Bone (Lates Calcarifer) with HCl Concentration Variance Gelatin is a food added ingredient used in emulsifiers, thickeners, food stabilizers. Gelatin is a type of protein in the form of gel obtained from the denaturation of skin, bone and fish tissue collagen denaturation. The process of making gelatin from the bones of white snapper (Lates Calcarifer) uses the HCl acid method with the parameters of yield, water content, ash content, and protein content. The immersion process carried out with varoius of HCl acid concentration 3%, 7% and 11%.The results show that the best value of% yield at a concentration of 7% of 1.90%, 10.16% water content. Ash content 3%. Protein content is 3.25%. FTIR spectrum revealed the presence of amida group at wave number 3269 cm-1, amide I; II; and Amida III sequentially at wave number 1656 cm-1; 1525.69 cm-1; 1161.15 cm-1.Keywords : Gelatin ; collagen ; Lates Calcarifer ; FTIRABSTRAK Gelatin merupakan bahan tambah pangan yang digunakan dalam pengemulsi, pengental, penstabil makanan. Gelatin merupakan salah satu jenis protein yang berbentuk gel yang didapatkan dari hasil denaturasi kolagen kulit, tulang dan jaringan ikan. Proses ekstrak gelatin dari bahan tulang ikan kakap putih (Lates Calcarifer) menggunakan asam HCl dengan parameter rendemen, kadar air, kadar abu, kadar protein dan analisis FTIR. Proses perendaman dilakukan variasi asam HCl 3%, 7% dan 11%. Dari hasil penelitian didapatkan nilai % rendemen tertinggi pada konsentrasi 7% sebesar 1,90%, dengan nilai kadar air 10,16%, kadar abu 3,00%, kadar protein 3,25%. Dari spektrum FTIR didapatkan gugus Amida A pada bilangan gelombang 3269 cm-1, amida I ; II ; dan amida III secara berturut-turut pada bilangan gelombang 1656 cm-1 ; 1525,69 cm-1 ; 1161,15 cm-1.Kata kunci : Gelatin ; ikan kakap putih ; FTI
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EKSTRAKSI GELATIN DARI TULANG IKAN KAKAP PUTIH (Lates Calcarifer) DENGAN VARIASI KONSENTRASI ASAM HCl
'Universitas Nusa Bangsa', 2020Co-Authors: Bhernama, Bhayu GitaAbstract:Extraction of Gelatin from Fish White Bone (Lates Calcarifer) with HCl Concentration Variance Gelatin is a food added ingredient used in emulsifiers, thickeners, food stabilizers. Gelatin is a type of protein in the form of gel obtained from the denaturation of skin, bone and fish tissue collagen denaturation. The process of making gelatin from the bones of white snapper (Lates Calcarifer) uses the HCl acid method with the parameters of yield, water content, ash content, and protein content. The immersion process carried out with varoius of HCl acid concentration 3%, 7% and 11%.The results show that the best value of% yield at a concentration of 7% of 1.90%, 10.16% water content. Ash content 3%. Protein content is 3.25%. FTIR spectrum revealed the presence of amida group at wave number 3269 cm-1, amide I; II; and Amida III sequentially at wave number 1656 cm-1; 1525.69 cm-1; 1161.15 cm-1.Keywords : Gelatin ; collagen ; Lates Calcarifer ; FTIRABSTRAK Gelatin merupakan bahan tambah pangan yang digunakan dalam pengemulsi, pengental, penstabil makanan. Gelatin merupakan salah satu jenis protein yang berbentuk gel yang didapatkan dari hasil denaturasi kolagen kulit, tulang dan jaringan ikan. Proses ekstrak gelatin dari bahan tulang ikan kakap putih (Lates Calcarifer) menggunakan asam HCl dengan parameter rendemen, kadar air, kadar abu, kadar protein dan analisis FTIR. Proses perendaman dilakukan variasi asam HCl 3%, 7% dan 11%. Dari hasil penelitian didapatkan nilai % rendemen tertinggi pada konsentrasi 7% sebesar 1,90%, dengan nilai kadar air 10,16%, kadar abu 3,00%, kadar protein 3,25%. Dari spektrum FTIR didapatkan gugus Amida A pada bilangan gelombang 3269 cm-1, amida I ; II ; dan amida III secara berturut-turut pada bilangan gelombang 1656 cm-1 ; 1525,69 cm-1 ; 1161,15 cm-1.Kata kunci : Gelatin ; ikan kakap putih ; FTI
Luc Grisez - One of the best experts on this subject based on the ideXlab platform.
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A Novel Virus Causes Scale Drop Disease in Lates Calcarifer
PLoS pathogens, 2015Co-Authors: Ad De Groof, Lars Guelen, Martin Deijs, Yorick Andreas Van Der Wal, Masato Miyata, Lotte Van Grinsven, Bartjan Simmelink, Yvonne Maria Johanna Corina Biermann, Luc GrisezAbstract:From 1992 onwards, outbreaks of a previously unknown illness have been reported in Asian seabass (Lates Calcarifer) kept in maricultures in Southeast Asia. The most striking symptom of this emerging disease is the loss of scales. It was referred to as scale drop syndrome, but the etiology remained enigmatic. By using a next-generation virus discovery technique, VIDISCA-454, sequences of an unknown virus were detected in serum of diseased fish. The near complete genome sequence of the virus was determined, which shows a unique genome organization, and low levels of identity to known members of the Iridoviridae. Based on homology of a series of putatively encoded proteins, the virus is a novel member of the Megalocytivirus genus of the Iridoviridae family. The virus was isolated and propagated in cell culture, where it caused a cytopathogenic effect in infected Asian seabass kidney and brain cells. Electron microscopy revealed icosahedral virions of about 140 nm, characteristic for the Iridoviridae. In vitro cultured virus induced scale drop syndrome in Asian seabass in vivo and the virus could be reisolated from these infected fish. These findings show that the virus is the causative agent for the scale drop syndrome, as each of Koch’s postuLates is fulfilled. We have named the virus Scale Drop Disease Virus. Vaccines prepared from BEI- and formalin inactivated virus, as well as from E. coli produced major capsid protein provide efficacious protection against scale drop disease.
