The Experts below are selected from a list of 240 Experts worldwide ranked by ideXlab platform
Hedayat Shirzad - One of the best experts on this subject based on the ideXlab platform.
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development of a Latex Agglutination Test as a simple and rapid method for diagnosis of trichomonas vaginalis infection
Avicenna journal of medical biotechnology, 2010Co-Authors: Hossein Yousofi Darani, Firuzeh Ahmadi, Nozhat Zabardast, Hossein Ali Yousefi, Hedayat ShirzadAbstract:Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a Latex Agglutination Test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichomonas vaginalis was harvested from TYIS33 culture medium and anti Trichomonas vaginalis antiserum was raised in rabbits. Salt precipitation method was used for antibody purification. Polyesteren Latex particles coated with purified antibody and used for detection of Trichomonas vaginalis. Clinical samples of vaginal discharge were collected from 500 women and examined for Trichomonas vaginalis by using wet mount, culture and Latex Agglutination Tests. Sensitivity and specificity of Latex Test was determined considering culture as golden standard. Sensitivity and specificity of Latex Agglutination Test was 100% and 81% and those of wet mount were 33.3% and 100%, respectively. Positive and negative predictive values of Latex Agglutination Test were 6% and 100%, respectively. Due to inconvenient sensitivity and specificity of the Latex Agglutination Test developed in this study, further work is recommended to improve the Test.
M. Hanif - One of the best experts on this subject based on the ideXlab platform.
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Large‐scale validation of a Latex Agglutination Test for diagnosis of tuberculosis
Fems Immunology and Medical Microbiology, 2003Co-Authors: Sangeeta Bhaskar, Jayant Nagesh Banavaliker, M. HanifAbstract:Large-scale validation of a simple Latex Agglutination Test for the diagnosis of tuberculosis is described. Soluble antigens extracted from a non-pathogenic saprophytic mycobacterium, Mycobacterium w, which shares antigenic determinants with Mycobacterium tuberculosis, were covalently linked to carboxylated polystyrene Latex beads. Batch to batch reproducibility of coated Latex was ensured. Latex reagents were standardized to overcome non-specific Agglutination. Reagents of the Test are stable for 1 year at 4°C. A total of 1058 serum samples of pulmonary and extrapulmonary tuberculosis patients or patients with other pulmonary diseases and healthy controls living in endemic areas were Tested. Sensitivity of 94% for pulmonary tuberculosis and 87% for extrapulmonary tuberculosis was obtained. Specificity is 92.2% for healthy controls and patients with other respiratory diseases. We conclude that the Latex Agglutination Test can be utilized for mass screening for both pulmonary and extrapulmonary tuberculosis where diagnosis by existing methods is much more difficult.
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large scale validation of a Latex Agglutination Test for diagnosis of tuberculosis
Fems Immunology and Medical Microbiology, 2003Co-Authors: Sangeeta Bhaskar, Jayant Nagesh Banavaliker, M. HanifAbstract:Large-scale validation of a simple Latex Agglutination Test for the diagnosis of tuberculosis is described. Soluble antigens extracted from a non-pathogenic saprophytic mycobacterium, Mycobacterium w, which shares antigenic determinants with Mycobacterium tuberculosis, were covalently linked to carboxylated polystyrene Latex beads. Batch to batch reproducibility of coated Latex was ensured. Latex reagents were standardized to overcome non-specific Agglutination. Reagents of the Test are stable for 1 year at 4°C. A total of 1058 serum samples of pulmonary and extrapulmonary tuberculosis patients or patients with other pulmonary diseases and healthy controls living in endemic areas were Tested. Sensitivity of 94% for pulmonary tuberculosis and 87% for extrapulmonary tuberculosis was obtained. Specificity is 92.2% for healthy controls and patients with other respiratory diseases. We conclude that the Latex Agglutination Test can be utilized for mass screening for both pulmonary and extrapulmonary tuberculosis where diagnosis by existing methods is much more difficult.
Hossein Yousofi Darani - One of the best experts on this subject based on the ideXlab platform.
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development of a Latex Agglutination Test as a simple and rapid method for diagnosis of trichomonas vaginalis infection
Avicenna journal of medical biotechnology, 2010Co-Authors: Hossein Yousofi Darani, Firuzeh Ahmadi, Nozhat Zabardast, Hossein Ali Yousefi, Hedayat ShirzadAbstract:Trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. In this study, development of a Latex Agglutination Test using native antigens for rapid diagnosis of trichomoniasis is investigated. Trichomonas vaginalis was harvested from TYIS33 culture medium and anti Trichomonas vaginalis antiserum was raised in rabbits. Salt precipitation method was used for antibody purification. Polyesteren Latex particles coated with purified antibody and used for detection of Trichomonas vaginalis. Clinical samples of vaginal discharge were collected from 500 women and examined for Trichomonas vaginalis by using wet mount, culture and Latex Agglutination Tests. Sensitivity and specificity of Latex Test was determined considering culture as golden standard. Sensitivity and specificity of Latex Agglutination Test was 100% and 81% and those of wet mount were 33.3% and 100%, respectively. Positive and negative predictive values of Latex Agglutination Test were 6% and 100%, respectively. Due to inconvenient sensitivity and specificity of the Latex Agglutination Test developed in this study, further work is recommended to improve the Test.
Huanchun Chen - One of the best experts on this subject based on the ideXlab platform.
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a rapid assay for detecting antibody against bluetongue virus with a Latex Agglutination Test using recombinant vp7 antigen
Journal of Veterinary Diagnostic Investigation, 2010Co-Authors: Junxing Yang, Huanchun Chen, Jianqiang Lv, Bing Chen, Zhouxi RuanAbstract:A Latex Agglutination Test (LAT) for detecting antibody against Bluetongue virus (BTV) in ruminants was developed using Latex beads coupled with recombinant VP7 protein. Compared with competitive enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the LAT were 99.0% and 93.0%, respectively. There was excellent agreement between the results obtained by competitive ELISA and the LAT (kappa = 0.930). Because it is rapid and easy to use, the LAT could be used for BTV antibody detection, especially for screening many serum samples.
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A slide Latex Agglutination Test for the rapid detection of antibodies in serum against porcine parvovirus.
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2006Co-Authors: J. Lü, J. Zhao, Liurong Fang, Qigai He, Huanchun ChenAbstract:Summary A slide Latex Agglutination Test (LAT) was developed and evaluated to detect serum antibodies against porcine parvovirus. Porcine parvovirus antigen was obtained by 10% PEG-6000 and 0.5 mol/l sodium chloride precipitation, and inactivated by 0.1% methanal. Two per cent suspensions of Latex particles (0.5–0.8 μm) were coated by adding an equal volume of porcine parvovirus antigen at 0.34 μg/ml. Repeatability of Latex Agglutination Test was evaluated with a panel of 100 sera using the same and different antigen lots. A good agreement between LAT and haemAgglutination inhibit assay was observed. Because of convenience and speed of performance, this method would be used widely in clinic examination.
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Latex Agglutination Test for monitoring antibodies to avian influenza virus subtype h5n1
Journal of Clinical Microbiology, 2005Co-Authors: Xiaojuan Xu, Zhengjun Yu, Hongchao Li, Huanchun ChenAbstract:A Latex Agglutination Test (LAT) based on polystyrene beads sensitized with inactivated avian influenza virus H5N1 particles was developed. Compared with the hemAgglutination inhibition Test, the sensitivity and specificity of the LAT were 88.8 and 97.6%, respectively, in detecting 830 serum samples from vaccinated chickens. The Test has application potential in field practice.
Sangeeta Bhaskar - One of the best experts on this subject based on the ideXlab platform.
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Large‐scale validation of a Latex Agglutination Test for diagnosis of tuberculosis
Fems Immunology and Medical Microbiology, 2003Co-Authors: Sangeeta Bhaskar, Jayant Nagesh Banavaliker, M. HanifAbstract:Large-scale validation of a simple Latex Agglutination Test for the diagnosis of tuberculosis is described. Soluble antigens extracted from a non-pathogenic saprophytic mycobacterium, Mycobacterium w, which shares antigenic determinants with Mycobacterium tuberculosis, were covalently linked to carboxylated polystyrene Latex beads. Batch to batch reproducibility of coated Latex was ensured. Latex reagents were standardized to overcome non-specific Agglutination. Reagents of the Test are stable for 1 year at 4°C. A total of 1058 serum samples of pulmonary and extrapulmonary tuberculosis patients or patients with other pulmonary diseases and healthy controls living in endemic areas were Tested. Sensitivity of 94% for pulmonary tuberculosis and 87% for extrapulmonary tuberculosis was obtained. Specificity is 92.2% for healthy controls and patients with other respiratory diseases. We conclude that the Latex Agglutination Test can be utilized for mass screening for both pulmonary and extrapulmonary tuberculosis where diagnosis by existing methods is much more difficult.
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large scale validation of a Latex Agglutination Test for diagnosis of tuberculosis
Fems Immunology and Medical Microbiology, 2003Co-Authors: Sangeeta Bhaskar, Jayant Nagesh Banavaliker, M. HanifAbstract:Large-scale validation of a simple Latex Agglutination Test for the diagnosis of tuberculosis is described. Soluble antigens extracted from a non-pathogenic saprophytic mycobacterium, Mycobacterium w, which shares antigenic determinants with Mycobacterium tuberculosis, were covalently linked to carboxylated polystyrene Latex beads. Batch to batch reproducibility of coated Latex was ensured. Latex reagents were standardized to overcome non-specific Agglutination. Reagents of the Test are stable for 1 year at 4°C. A total of 1058 serum samples of pulmonary and extrapulmonary tuberculosis patients or patients with other pulmonary diseases and healthy controls living in endemic areas were Tested. Sensitivity of 94% for pulmonary tuberculosis and 87% for extrapulmonary tuberculosis was obtained. Specificity is 92.2% for healthy controls and patients with other respiratory diseases. We conclude that the Latex Agglutination Test can be utilized for mass screening for both pulmonary and extrapulmonary tuberculosis where diagnosis by existing methods is much more difficult.
