The Experts below are selected from a list of 387 Experts worldwide ranked by ideXlab platform

Weber Leal De Moura - One of the best experts on this subject based on the ideXlab platform.

  • Morfologia das células do sangue periférico em emas (Rhea americana)
    Brazilian Journal of Veterinary Research and Animal Science, 2009
    Co-Authors: Eunice Anita De Moura Fortes, Airton Mendes Conde Júnior, Antônio Francisco De Sousa, Ezequiel Cardozo Saraiva De Almeida, Weber Leal De Moura
    Abstract:

    The rhea (Rhea americana) is a South American bird of the ratite group and of the Rheiformes order. It has been exploited for economical purposes, as cattle alternative in European and South American countries. In Brazil, the State of Rio Grande do Sul is outstanding, in rhea rearing and it is in the process of implantation in the Country Northeasty Region. This work aims to describe the morphology of the blood cells in rheas. In this work ten rheas were used, regardless age and sex. Two ml of peripheral blood were collected by puncture of the brachial vein with disposable syringe. The samples here partially used to make extensions with Leishman Stain. Seven types of nucleate cells have been observed through morphologic analysis on the light microscope. The erythrocyte revealed an elliptical form, with condensed nucleus of elliptical form; acidophilic cytoplasm. The thrombocyte revealed an elliptical form, with nucleus located in one of the polar regions; pale cytoplasm. As to the round-shaped leukocytes, within the granulocytes, the heterophils presented excentric, condensed, and lobulated nucleus; cytoplasm rich in fusiform salmon-colored granules. The eosinophils distinguish from the heterophils due to the round eosinophilic granules. The basophils stand out from the other granulocytes due to its large and central nucleus with round specific cytoplasmic and highly basophilic granules. Within the agranulocytes, the monocytes presented reniform nucleus, which is frequently central, with slack chromatin, with small areas of condensation; cytoplasm lightly basophilic and with vacuoles. The lymphocytes presented varies forms and sizes; large nucleus with slack chromatin with some nucleoli; scarce and basophilic cytoplasm. The cells of the peripheral blood of Rhea americana present on the light microscope morphology similar to the other birds which have already been studied.

  • Morfologia das células do sangue periférico em emas (Rhea americana)
    Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia, 2009
    Co-Authors: Fortes, Eunice Anita De Moura, Antônio Francisco De Sousa, Ezequiel Cardozo Saraiva De Almeida, Conde Júnior, Aírton Mendes, Weber Leal De Moura
    Abstract:

    The rhea (Rhea americana) is a South American bird of the ratite group and of the Rheiformes order. It has been exploited for economical purposes, as cattle alternative in European and South American countries. In Brazil, the State of Rio Grande do Sul is outstanding, in rhea rearing and it is in the process of implantation in the Country Northeasty Region. This work aims to describe the morphology of the blood cells in rheas. In this work ten rheas were used, regardless age and sex. Two ml of peripheral blood were collected by puncture of the brachial vein with disposable syringe. The samples here partially used to make extensions with Leishman Stain. Seven types of nucleate cells have been observed through morphologic analysis on the light microscope. The erythrocyte revealed an elliptical form, with condensed nucleus of elliptical form; acidophilic cytoplasm. The thrombocyte revealed an elliptical form, with nucleus located in one of the polar regions; pale cytoplasm. As to the round-shaped leukocytes, within the granulocytes, the heterophils presented excentric, condensed, and lobulated nucleus; cytoplasm rich in fusiform salmon-colored granules. The eosinophils distinguish from the heterophils due to the round eosinophilic granules. The basophils stand out from the other granulocytes due to its large and central nucleus with round specific cytoplasmic and highly basophilic granules. Within the agranulocytes, the monocytes presented reniform nucleus, which is frequently central, with slack chromatin, with small areas of condensation; cytoplasm lightly basophilic and with vacuoles. The lymphocytes presented varies forms and sizes; large nucleus with slack chromatin with some nucleoli; scarce and basophilic cytoplasm. The cells of the peripheral blood of Rhea americana present on the light microscope morphology similar to the other birds which have already been studied.A ema (Rhea americana) é uma ave sul-americana do grupo das ratitas, da ordem Rheiforme, freqüentemente explorada com fins econômicos, como pecuária alternativa em países europeus e sul-americanos. No Brasil, destaca-se o Rio Grande do Sul e, em fase inicial, o Nordeste. O presente estudo objetivou descrever a morfologia das células sangüíneas em ema. Neste trabalho foram utilizados dez exemplares, desconsiderando-se idade e sexo. Foram colhidos 3mL de sangue periférico por punção da veia braquial, com seringa descartável. As amostras foram utilizadas, em parte, na confecção de extensões coradas com Leishman. Feita a análise morfológica ao Microscópio de Luz, foram observados sete tipos celulares nucleados. O eritrócito mostrou-se elíptico, com núcleo geralmente condensado, de forma elíptica; citoplasma acidófilo. O trombócito apresentou-se elíptico, com núcleo localizado em um dos pólos; citoplasma pálido. Quanto aos leucócitos, de forma arredondada, entre os granulócitos os heterófilos apresentaram-se com núcleo excêntrico, condensado, lobulado; citoplasma rico em grânulos fusiformes de coloração salmão. Os eosinófilos distinguem-se dos heterófilos pelos grânulos arredondados eosinofílicos. Os basófilos destacam-se dos outros granulócitos pelo núcleo grande e central, com grânulos citoplasmáticos específicos arredondados e fortemente basofílicos. Entre os agranulócitos, os monócitos mostraram núcleo rineforme, freqüentemente central, de cromatina frouxa, com pequenas áreas de condensação; citoplasma levemente basofílico e com vacúolos. Os linfócitos apresentaram-se variados em forma e tamanho; núcleo grande com cromatina frouxa, com alguns nucléolos; citoplasma escasso e basofílico. As células do sangue periférico de Rhea americana apresentam ao Microscópio de Luz morfologia semelhante às demais aves já estudadas

Arjen M Dondorp - One of the best experts on this subject based on the ideXlab platform.

  • comparing Leishman and giemsa Staining for the assessment of peripheral blood smear preparations in a malaria endemic region in india
    Malaria Journal, 2014
    Co-Authors: Sanghamitra Sathpathi, Akshaya K Mohanty, Parthasarathi Satpathi, Saroj Kanta Mishra, Prativa Kumari Behera, Goutam Patel, Arjen M Dondorp
    Abstract:

    Microscopy of peripheral blood thin and thick films remains the reference for malaria diagnosis. Although Giemsa Staining is most commonly used, the Leishman Staining method provides better visualization of the nuclear chromatin pattern of cells. It is less well known whether accuracy of parasitaemia assessment is equally accurate with the latter method. Peripheral blood thin and thick smears from consecutive febrile patients admitted to Ispat General hospital, Rourkela, Odhisa, India, were Stained with Giemsa and Leishman Stain. Methods were compared for species identification, parasite quantification, and ability for identification of alternative diagnoses. Blood films from 1,180 fever patients were compared according to Staining method, of which 111 were identified as parasitaemic using Giemsa and 110 with Leishman Staining. The Kappa value as a measure of agreement between methods was 0.995 (p < 0.001), and the log10parasitaemia between methods were strongly correlated (r2 = 0.9981). In parasite negative patients, thin smear assessment contributed to making a diagnosis in 276/1,180 (23%) of cases. These assessments were better made in Leishman-Stained preparations, especially for the assessment of morphological changes in red and white cells. Leishman’s Staining method for thin and thick smears is a good alternative to Giemsa’s Stain for identifying Plasmodium parasites. The Leishman method is superior for visualization of red and white blood cell morphology.

Caetano, Flavio Henrique - One of the best experts on this subject based on the ideXlab platform.

  • Effects of exposition to polluted environments on blood cells of the fish Prochilodus lineatus
    Wiley-Blackwell, 2012
    Co-Authors: Pereira, Bruno Fiorelini, Da Silva Alves, Rebeca Mamede, Pitol, Dimitrius Leonardo, Senhorini, Jose Augusto, Gimenes De Alcantara Rocha, Rita De Cassia, Caetano, Flavio Henrique
    Abstract:

    In this study, we investigated the effects of contaminated water on the blood parameters of the fish Prochilodus lineatus exposed during 7 and 20 days. Blood was collected with heparinized syringes, and blood smears were prepared and Stained with Leishman Stain. Slides were examined and photographed using a Leica light microscope. Total white blood cell counts and differential counts of thrombocytes and leukocytes were obtained for statistical analysis. The group exposed to water samples from Lago Azul exhibited an increase in the number of leukocytes and in the total number of white blood cells, suggesting that the chemical contaminants in this environment were acting similarly to antigens in this fish species, causing the proliferation of defense cells. In the group exposed to detergent during 20 days, the number of thrombocytes decreased. These results suggest that the variations in the number of leukocytes were indicators of environmental pollution and that biodegradable detergents may, after a certain time of exposure, affect vital functions in fish, such as coagulation and prevention of infections, which directly involves thrombocytes. Microsc. Res. Tech., 2012. (c) 2011 Wiley Periodicals, Inc.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

  • Effects of exposition to polluted environments on blood cells of the fish Prochilodus lineatus
    WILEY-BLACKWELL, 2012
    Co-Authors: Pereira, Bruno Fiorelini, Da Silva Alves, Rebeca Mamede, Pitol, Dimitrius Leonardo, Senhorini, Jose Augusto, Gimenes De Alcantara Rocha, Rita De Cassia, Caetano, Flavio Henrique
    Abstract:

    In this study, we investigated the effects of contaminated water on the blood parameters of the fish Prochilodus lineatus exposed during 7 and 20 days. Blood was collected with heparinized syringes, and blood smears were prepared and Stained with Leishman Stain. Slides were examined and photographed using a Leica light microscope. Total white blood cell counts and differential counts of thrombocytes and leukocytes were obtained for statistical analysis. The group exposed to water samples from Lago Azul exhibited an increase in the number of leukocytes and in the total number of white blood cells, suggesting that the chemical contaminants in this environment were acting similarly to antigens in this fish species, causing the proliferation of defense cells. In the group exposed to detergent during 20 days, the number of thrombocytes decreased. These results suggest that the variations in the number of leukocytes were indicators of environmental pollution and that biodegradable detergents may, after a certain time of exposure, affect vital functions in fish, such as coagulation and prevention of infections, which directly involves thrombocytes. Microsc. Res. Tech., 2012. (c) 2011 Wiley Periodicals, Inc

  • Effects of exposition to polluted environments on blood cells of the fish Prochilodus lineatus
    MALDEN, 2012
    Co-Authors: Pereira, Bruno Fiorelini, Da Silva Alves, Rebeca Mamede, Pitol, Dimitrius Leonardo, Senhorini, Jose Augusto, Gimenes De Alcantara Rocha, Rita De Cassia, Caetano, Flavio Henrique
    Abstract:

    In this study, we investigated the effects of contaminated water on the blood parameters of the fish Prochilodus lineatus exposed during 7 and 20 days. Blood was collected with heparinized syringes, and blood smears were prepared and Stained with Leishman Stain. Slides were examined and photographed using a Leica light microscope. Total white blood cell counts and differential counts of thrombocytes and leukocytes were obtained for statistical analysis. The group exposed to water samples from Lago Azul exhibited an increase in the number of leukocytes and in the total number of white blood cells, suggesting that the chemical contaminants in this environment were acting similarly to antigens in this fish species, causing the proliferation of defense cells. In the group exposed to detergent during 20 days, the number of thrombocytes decreased. These results suggest that the variations in the number of leukocytes were indicators of environmental pollution and that biodegradable detergents may, after a certain time of exposure, affect vital functions in fish, such as coagulation and prevention of infections, which directly involves thrombocytes. Microsc. Res. Tech., 2012. (c) 2011 Wiley Periodicals, Inc.FAPESP [2009/17118-9]FAPES

Indira Kariveettil - One of the best experts on this subject based on the ideXlab platform.

  • a comparative study between conventional and modified Leishman Stain
    International Journal of Research, 2021
    Co-Authors: Rizwana Abdul Hye, Bindiya Gisuthan, Indira Kariveettil
    Abstract:

    Introduction: Leishman Stain has been used as the Stain of choice for peripheral blood films since many decades .But it has a disadvantage of consuming 15 minutes for the procedure alone thereby increasing the turn around time of peripheral smear reporting. In this study modified Leishman Stain was made by adding phenol to conventional Leishman to reduce the Staining time to 3 minutes without interfering with the quality of Stain. Aim: To study the quality of modified Leishman Stain in comparison with conventional preparation on peripheral blood smears. Materials and Methods: The present cross-sectional study was carried out in Central Haematology laboratory of a tertiary health care centre in Southern India. A pilot study was done to determine the ideal ratio of phenol to Leishman powder in preparing modified Stain without compromising the Staining quality. After obtaining consent, the blood sample was collected in anticoagulated vials. Sample size was calculated to be 85.Two thin wedge smears were prepared from each sample and one was Stained with conventional Leishman Stain and other with modified Leishman Stain. The Staining characteristics were assessed and scored by an experienced pathologist in terms of 6 parameters- RBC pattern, nuclear pattern, neutrophil granules, eosinophil granules, platelets and background Staining. The grades were 1, 2, 3 & 4 (poor, satisfied, good and excellent). Smears each with grades 2, 3 and 4 were selected for study. The results were analysed by Statistical Package for the Social Sciences (SPSS) software version 16.0. Result: The mean value for the Staining quality using conventional method was 4.73 and for modified method was 5.39, and the total score possible is 6. Photomicrograph showed excellent results with modified Leishman Stain. Thus from these values we can interpret that modified method gave much more acceptable results than that of conventional method. Conclusion: Unlike the conventional method which requires a total of 15 minutes, to complete the Staining process, modified Leishman Staining techniques takes only 3 minutes. Blood films can be Stained within a short period of time thus aiding in rapid diagnosis and treatment of patients.

Antônio Francisco De Sousa - One of the best experts on this subject based on the ideXlab platform.

  • Morfologia das células do sangue periférico em emas (Rhea americana)
    Brazilian Journal of Veterinary Research and Animal Science, 2009
    Co-Authors: Eunice Anita De Moura Fortes, Airton Mendes Conde Júnior, Antônio Francisco De Sousa, Ezequiel Cardozo Saraiva De Almeida, Weber Leal De Moura
    Abstract:

    The rhea (Rhea americana) is a South American bird of the ratite group and of the Rheiformes order. It has been exploited for economical purposes, as cattle alternative in European and South American countries. In Brazil, the State of Rio Grande do Sul is outstanding, in rhea rearing and it is in the process of implantation in the Country Northeasty Region. This work aims to describe the morphology of the blood cells in rheas. In this work ten rheas were used, regardless age and sex. Two ml of peripheral blood were collected by puncture of the brachial vein with disposable syringe. The samples here partially used to make extensions with Leishman Stain. Seven types of nucleate cells have been observed through morphologic analysis on the light microscope. The erythrocyte revealed an elliptical form, with condensed nucleus of elliptical form; acidophilic cytoplasm. The thrombocyte revealed an elliptical form, with nucleus located in one of the polar regions; pale cytoplasm. As to the round-shaped leukocytes, within the granulocytes, the heterophils presented excentric, condensed, and lobulated nucleus; cytoplasm rich in fusiform salmon-colored granules. The eosinophils distinguish from the heterophils due to the round eosinophilic granules. The basophils stand out from the other granulocytes due to its large and central nucleus with round specific cytoplasmic and highly basophilic granules. Within the agranulocytes, the monocytes presented reniform nucleus, which is frequently central, with slack chromatin, with small areas of condensation; cytoplasm lightly basophilic and with vacuoles. The lymphocytes presented varies forms and sizes; large nucleus with slack chromatin with some nucleoli; scarce and basophilic cytoplasm. The cells of the peripheral blood of Rhea americana present on the light microscope morphology similar to the other birds which have already been studied.

  • Morfologia das células do sangue periférico em emas (Rhea americana)
    Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia, 2009
    Co-Authors: Fortes, Eunice Anita De Moura, Antônio Francisco De Sousa, Ezequiel Cardozo Saraiva De Almeida, Conde Júnior, Aírton Mendes, Weber Leal De Moura
    Abstract:

    The rhea (Rhea americana) is a South American bird of the ratite group and of the Rheiformes order. It has been exploited for economical purposes, as cattle alternative in European and South American countries. In Brazil, the State of Rio Grande do Sul is outstanding, in rhea rearing and it is in the process of implantation in the Country Northeasty Region. This work aims to describe the morphology of the blood cells in rheas. In this work ten rheas were used, regardless age and sex. Two ml of peripheral blood were collected by puncture of the brachial vein with disposable syringe. The samples here partially used to make extensions with Leishman Stain. Seven types of nucleate cells have been observed through morphologic analysis on the light microscope. The erythrocyte revealed an elliptical form, with condensed nucleus of elliptical form; acidophilic cytoplasm. The thrombocyte revealed an elliptical form, with nucleus located in one of the polar regions; pale cytoplasm. As to the round-shaped leukocytes, within the granulocytes, the heterophils presented excentric, condensed, and lobulated nucleus; cytoplasm rich in fusiform salmon-colored granules. The eosinophils distinguish from the heterophils due to the round eosinophilic granules. The basophils stand out from the other granulocytes due to its large and central nucleus with round specific cytoplasmic and highly basophilic granules. Within the agranulocytes, the monocytes presented reniform nucleus, which is frequently central, with slack chromatin, with small areas of condensation; cytoplasm lightly basophilic and with vacuoles. The lymphocytes presented varies forms and sizes; large nucleus with slack chromatin with some nucleoli; scarce and basophilic cytoplasm. The cells of the peripheral blood of Rhea americana present on the light microscope morphology similar to the other birds which have already been studied.A ema (Rhea americana) é uma ave sul-americana do grupo das ratitas, da ordem Rheiforme, freqüentemente explorada com fins econômicos, como pecuária alternativa em países europeus e sul-americanos. No Brasil, destaca-se o Rio Grande do Sul e, em fase inicial, o Nordeste. O presente estudo objetivou descrever a morfologia das células sangüíneas em ema. Neste trabalho foram utilizados dez exemplares, desconsiderando-se idade e sexo. Foram colhidos 3mL de sangue periférico por punção da veia braquial, com seringa descartável. As amostras foram utilizadas, em parte, na confecção de extensões coradas com Leishman. Feita a análise morfológica ao Microscópio de Luz, foram observados sete tipos celulares nucleados. O eritrócito mostrou-se elíptico, com núcleo geralmente condensado, de forma elíptica; citoplasma acidófilo. O trombócito apresentou-se elíptico, com núcleo localizado em um dos pólos; citoplasma pálido. Quanto aos leucócitos, de forma arredondada, entre os granulócitos os heterófilos apresentaram-se com núcleo excêntrico, condensado, lobulado; citoplasma rico em grânulos fusiformes de coloração salmão. Os eosinófilos distinguem-se dos heterófilos pelos grânulos arredondados eosinofílicos. Os basófilos destacam-se dos outros granulócitos pelo núcleo grande e central, com grânulos citoplasmáticos específicos arredondados e fortemente basofílicos. Entre os agranulócitos, os monócitos mostraram núcleo rineforme, freqüentemente central, de cromatina frouxa, com pequenas áreas de condensação; citoplasma levemente basofílico e com vacúolos. Os linfócitos apresentaram-se variados em forma e tamanho; núcleo grande com cromatina frouxa, com alguns nucléolos; citoplasma escasso e basofílico. As células do sangue periférico de Rhea americana apresentam ao Microscópio de Luz morfologia semelhante às demais aves já estudadas