The Experts below are selected from a list of 297 Experts worldwide ranked by ideXlab platform

Alireza Foroumadi - One of the best experts on this subject based on the ideXlab platform.

Mina Behrouzifardmoghadam - One of the best experts on this subject based on the ideXlab platform.

Hannah Akuffo - One of the best experts on this subject based on the ideXlab platform.

  • vivo evaluation of immune responses in Leishmaniasis: the use of cross-species leishmanin preparations for skin testing
    1995
    Co-Authors: Hannah Akuffo, Martha Darce, Andtebebe Yamane Berhan
    Abstract:

    Abstract. Skin test reactivity to two commercial preparations of Leishmania major leishmanin was evaluated in Leishmaniasis patents from Ethiopia (L. aethiopica) and Nicaragua (probably L. braziliensis complex). The purpose of using different preparations of L. major was to evaluate whether L. major skin test antigens could generally be used to detect Leishmaniasis due to L. aethiopica and L braziliensis. One preparation was superior in identifying the majority (83-90%) of confirmed cases of local cutaneous Leishmaniasis (LCL) from Ethiopia. In vitro responses to promastigotes of Leishmania spp. correlated well with leishmanin positivity to this preparation. The skin test antigen, which performed less well (showing a positive result in only 50 % of the LCL patents), showed promise when used to test active and cured cases of Leishmaniasis in Nicaragua (positive result in 92 % of the active and cured patents). Cross-reacting Leishmania spp. may be considered for use in the preparation of standardized leishmanin antigen; however, differences in the commercial preparation or the diluent in which the parasites are prepared may affect antigenicity and thus its efficacy in detecting different forms of Leishmaniasis in different geographic areas. Parasites of the genus Leishmania cause life-threatening visceral or disfiguring cutaneous Leishmaniasis in humans. Resistance to Leishmanial infection in human and mouse models has been shown to be through the development o

  • mechanisms of resistance to Leishmania aethiopica i interferon γ in combination with a cytokine not tumor necrosis factor α is required but cannot act alone in the inhibition of intracellular forms of l aethiopica in thp1 cells
    European Journal of Immunology, 1992
    Co-Authors: Tesema Taye, Abdi Muse Mohamed, Hannah Akuffo
    Abstract:

    Following exposure to promastigotes of various Leishmania species, mononuclear cells from non-exposed as well as potentially exposed individuals produced a cytokine response which inhibited intracellular forms of Leishmania aethiopica in a permissive monocytic cell line (THP1). Interferon-gamma (IFN-gamma), was one of the cytokines responsible for this anti-Leishmanial effect. IFN-gamma was necessary for inhibition but could not act on its own inhibiting L. aethiopica. Tumor necrosis factor-alpha seemed not to be involved in the anti-L. aethiopica effect. The observed effects were in the absence of endotoxin. The results suggest that the mechanisms of killing of L. aethiopica in human cells may differ from those responsible for inhibition of other Leishmania parasites (such as Leishmania major) in mouse macrophages. Furthermore, that potentially relevant responses to Leishmania antigens may exist in normal individuals.

  • Mechanisms of resistance to Leishmania aethiopica. I. Interferon‐γ in combination with a cytokine (not tumor necrosis factor‐α) is required, but cannot act alone in the inhibition of intracellular forms of L. aethiopica in THP1 cells
    European journal of immunology, 1992
    Co-Authors: Abdi Muse Mohamed, Tesema Taye, Hannah Akuffo
    Abstract:

    Following exposure to promastigotes of various Leishmania species, mononuclear cells from non-exposed as well as potentially exposed individuals produced a cytokine response which inhibited intracellular forms of Leishmania aethiopica in a permissive monocytic cell line (THP1). Interferon-gamma (IFN-gamma), was one of the cytokines responsible for this anti-Leishmanial effect. IFN-gamma was necessary for inhibition but could not act on its own inhibiting L. aethiopica. Tumor necrosis factor-alpha seemed not to be involved in the anti-L. aethiopica effect. The observed effects were in the absence of endotoxin. The results suggest that the mechanisms of killing of L. aethiopica in human cells may differ from those responsible for inhibition of other Leishmania parasites (such as Leishmania major) in mouse macrophages. Furthermore, that potentially relevant responses to Leishmania antigens may exist in normal individuals.

Abdi Muse Mohamed - One of the best experts on this subject based on the ideXlab platform.

Abbas Shafiee - One of the best experts on this subject based on the ideXlab platform.