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Indu Shekhar Thakur - One of the best experts on this subject based on the ideXlab platform.
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Proteomic characterization and schizophyllan production by Schizophyllum commune ISTL04 cultured on Leucaena leucocephala wood under submerged fermentation.
Bioresource technology, 2017Co-Authors: Manoj Kumar Singh, Madan Kumar, Indu Shekhar ThakurAbstract:In this study Schizophyllum commune ISTL04 was grown on Leucaena leucocephala wood (LLW) for secretome analysis and schizophyllan production. There is no report on extracellular protein profile and schizophyllan production on woody biomass by this fungus under submerged fermentation conditions. Leucaena leucocephala, a promising bioenergy crop having high holocellulose content was used as substrate without pretreatment. The maximum sugar, extracellular protein and exopolysaccharide (EPS) production during fermentation was found to be 8.53±0.07mgmL-1, 391±7.51mgL-1 and 4.2±0.1gL-1 or 0.21gg-1LLW on day 18 respectively. The secretome profile was dominated by glycoside hydrolases followed by carbohydrate esterase and other oxidative enzymes. EPS was further characterized by FTIR and GC-MS for functional group, monomer composition and linkage analysis and was identified as schizophyllan. The result indicated that LLW can be utilized as a low cost substrate for enzyme cocktail and schizophyllan production.
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novel lipase from basidiomycetes schizophyllum commune istl04 produced by solid state fermentation of Leucaena leucocephala seeds
Journal of Molecular Catalysis B-enzymatic, 2014Co-Authors: Manoj Singh, Madan Kumar, Jyoti Singh, Indu Shekhar ThakurAbstract:Abstract The present investigation reports a novel thermoalkalotolerant lipase producing Basidiomycetes, Schizophyllum commune ISTL04 for the first time. Abundantly available Leucaena leucocephala seeds were exploited as a cheap fermentable source for the feasible production of extracellular lipase. The isolate ISTL04 grew vigorously on Leucaena seeds in comparison to Soybean meal and Wheat bran exhibiting a lipase activity of 146.5 U/g. The S. commune ISTL04 (SCI) lipase was purified to 35.76 folds with a specific activity of 238.13 U/mg and an estimated mass of 60 kDa. The temperature and pH optima for lipolytic activity were 60 °C and 11, respectively. The enzyme exhibited remarkable stability in the presence of non-polar and polar organic solvents (50%, v/v), retaining more than 90% and 40% activity, respectively. The lipase activity increased significantly in the presence of Mg 2+ (154%), Ca 2+ (109.7%), Mn 2+ (106%) and Tween 80 (240%). The distinctive and broader operational properties of the SCI lipase make it a promising biocatalyst for various industrial processes.
Saud I Alresayes - One of the best experts on this subject based on the ideXlab platform.
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Leucaena leucocephala lam de wit seed oil characterization and uses
Industrial Crops and Products, 2014Co-Authors: Imededdine Arbi Nehdi, Hassen Mohamed Sbihi, Chin Ping Tan, Saud I AlresayesAbstract:Abstract Leucaena leucocephala (Lam.) de Wit (coffee bush) is a strictly tropical species that requires warm temperatures for optimal growth. It is drought-tolerant (even during establishment) and can withstand up to 7 months of dry season. L. leucocephala oil extracted from seeds was analyzed to determine it possible uses. The oil content of the seeds was 5.44% and mainly consisted of linoleic acid (51.65%), oleic acid (21.40%), palmitic acid (13.21%) and stearic acid (5.92%). This oil has high vitamin E activity because of its concentration of α-tocopherol (175.47 mg/100 g). The weak acid and peroxide values (0.6% and 3.56 mequiv.·O 2 /kg oil, respectively) were indicators of the good quality of the oil. L. leucocephala showed high thermal stability (up to 255.47 °C) and some absorbance in the UV-B and UV-C ranges. The results showed that this new seed oil can be used as an ingredient in cosmetic or pharmaceutical preparations.
Imededdine Arbi Nehdi - One of the best experts on this subject based on the ideXlab platform.
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Leucaena leucocephala lam de wit seed oil characterization and uses
Industrial Crops and Products, 2014Co-Authors: Imededdine Arbi Nehdi, Hassen Mohamed Sbihi, Chin Ping Tan, Saud I AlresayesAbstract:Abstract Leucaena leucocephala (Lam.) de Wit (coffee bush) is a strictly tropical species that requires warm temperatures for optimal growth. It is drought-tolerant (even during establishment) and can withstand up to 7 months of dry season. L. leucocephala oil extracted from seeds was analyzed to determine it possible uses. The oil content of the seeds was 5.44% and mainly consisted of linoleic acid (51.65%), oleic acid (21.40%), palmitic acid (13.21%) and stearic acid (5.92%). This oil has high vitamin E activity because of its concentration of α-tocopherol (175.47 mg/100 g). The weak acid and peroxide values (0.6% and 3.56 mequiv.·O 2 /kg oil, respectively) were indicators of the good quality of the oil. L. leucocephala showed high thermal stability (up to 255.47 °C) and some absorbance in the UV-B and UV-C ranges. The results showed that this new seed oil can be used as an ingredient in cosmetic or pharmaceutical preparations.
S Pramod - One of the best experts on this subject based on the ideXlab platform.
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immunolocalization of β 1 4 d galactan xyloglucans and xylans in the reaction xylem fibres of Leucaena leucocephala lam de wit
Plant Physiology and Biochemistry, 2019Co-Authors: S Pramod, Kishore S Rajput, K S RaoAbstract:Cell wall architecture of tension wood fibres represents a suitable biological system to study the mechanism of growth and maintenance of posture of trees growing under various physical and physiological growth constraints. In the present study, we investigated the spatial distributions of β-(1-4)-D-galactan, xyloglucan and xylans (both less and highly substituted) in the opposite and tension wood fibres of bent Leucaena leucocephala by immunolabelling with monoclonal antibodies LM5, CCRCM1, LM10 and LM11 specific to these carbohydrate epitopes. The presence of non-lignified, tertiary wall layer is the typical tension wood characteristic associated with the reaction xylem fibres in Leucaena. LM5 labelling of opposite fibres showed weak labelling in the cell walls indicating less concentration of β-(1-4)-D-galactans while tension wood showed strong labelling in the tertiary wall layer suggesting the gelatinous layer (G-layer) has a strong cross linking with β-(1-4)-D-galactans. Xyloglucan distribution was more in the compound middle lamellae and the primary wall-S1 layer boundary of tension wood fibres as compared to that of opposite wood. A weak labelling was also evident near the boundary between the G-layer and the secondary wall of tension wood fibres. The secondary wall of opposite and tension wood fibres showed a strong distribution of both ls ACG Xs (LM10) and hs ACG Xs (LM11) while a weak labelling was noticed in the compound middle lamella. Tension wood fibres also showed strong xylan labelling mainly confined to the lignified secondary walls while the G-layer showed weak xylan labelling. In conclusion, our results suggest that β-(1-4)-D-galactans and xyloglucans could be implicated in the tensile stress generation within the G-layer of tension wood fibres of Leucaena leucocephala.
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structural histochemical and chemical characterization of normal tension and opposite wood of subabul Leucaena leucocephala lam de wit
Wood Science and Technology, 2013Co-Authors: S Pramod, Anna SundbergAbstract:Structure, histochemistry and chemical composition of tension (TW), opposite (OW) and normal wood (NW) of Leucaena leucocephala have been studied using histological, histochemical and biochemical methods. TW vessels are longer and have a larger diameter than NW vessels, and the rays are shorter and thinner. The G-layer replaced S3 and parts of S2 wall layers in TW fibres. TW further contained less lignin that also had a lower syringyl-to-guaiacyl ratio. The lignin was also more condensed. The content of α-cellulose was high in TW. TW contains hemicelluloses and pectins composed of more galactose, and less xylose, mannose, 4-O-methylglucuronic acid and galacturonic acid compared to that of OW. This study suggests that in the TW of Leucaena, the amount and composition of lignin, hemicelluloses and pectins differ considerably from that in OW and NW.
Madan Kumar - One of the best experts on this subject based on the ideXlab platform.
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Proteomic characterization and schizophyllan production by Schizophyllum commune ISTL04 cultured on Leucaena leucocephala wood under submerged fermentation.
Bioresource technology, 2017Co-Authors: Manoj Kumar Singh, Madan Kumar, Indu Shekhar ThakurAbstract:In this study Schizophyllum commune ISTL04 was grown on Leucaena leucocephala wood (LLW) for secretome analysis and schizophyllan production. There is no report on extracellular protein profile and schizophyllan production on woody biomass by this fungus under submerged fermentation conditions. Leucaena leucocephala, a promising bioenergy crop having high holocellulose content was used as substrate without pretreatment. The maximum sugar, extracellular protein and exopolysaccharide (EPS) production during fermentation was found to be 8.53±0.07mgmL-1, 391±7.51mgL-1 and 4.2±0.1gL-1 or 0.21gg-1LLW on day 18 respectively. The secretome profile was dominated by glycoside hydrolases followed by carbohydrate esterase and other oxidative enzymes. EPS was further characterized by FTIR and GC-MS for functional group, monomer composition and linkage analysis and was identified as schizophyllan. The result indicated that LLW can be utilized as a low cost substrate for enzyme cocktail and schizophyllan production.
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novel lipase from basidiomycetes schizophyllum commune istl04 produced by solid state fermentation of Leucaena leucocephala seeds
Journal of Molecular Catalysis B-enzymatic, 2014Co-Authors: Manoj Singh, Madan Kumar, Jyoti Singh, Indu Shekhar ThakurAbstract:Abstract The present investigation reports a novel thermoalkalotolerant lipase producing Basidiomycetes, Schizophyllum commune ISTL04 for the first time. Abundantly available Leucaena leucocephala seeds were exploited as a cheap fermentable source for the feasible production of extracellular lipase. The isolate ISTL04 grew vigorously on Leucaena seeds in comparison to Soybean meal and Wheat bran exhibiting a lipase activity of 146.5 U/g. The S. commune ISTL04 (SCI) lipase was purified to 35.76 folds with a specific activity of 238.13 U/mg and an estimated mass of 60 kDa. The temperature and pH optima for lipolytic activity were 60 °C and 11, respectively. The enzyme exhibited remarkable stability in the presence of non-polar and polar organic solvents (50%, v/v), retaining more than 90% and 40% activity, respectively. The lipase activity increased significantly in the presence of Mg 2+ (154%), Ca 2+ (109.7%), Mn 2+ (106%) and Tween 80 (240%). The distinctive and broader operational properties of the SCI lipase make it a promising biocatalyst for various industrial processes.