The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform

Myra O Mcclure - One of the best experts on this subject based on the ideXlab platform.

Otto Erlwein - One of the best experts on this subject based on the ideXlab platform.

J W Casey - One of the best experts on this subject based on the ideXlab platform.

  • Bovine Leukemia Virus gene expression in vivo.
    Journal of virology, 1992
    Co-Authors: L Haas, T Divers, J W Casey
    Abstract:

    The in vivo transcriptional status of bovine Leukemia Virus was assessed at three stages of infection during the progression of the disease: aleukemic stage, persistent lymphocytosis, and Leukemia/lymphosarcoma. Bovine Leukemia Virus transcripts could be amplified from total or cytoplasmic enriched lymphocyte RNA by reverse transcription polymerase chain reaction in cells from all but a few aleukemic animals. With primer pairs diagnostic for differentially spliced transcripts (full length-genomic, envelope, tax/rex, and alternatively spliced), a trend toward exclusion of both full-length and envelope RNAs, with retention of the tax/rex message, appears as Leukemia/lymphosarcoma develops.

Ruth M Ruprecht - One of the best experts on this subject based on the ideXlab platform.

  • Phylogenetic Relationship of the Complete Rauscher Murine Leukemia Virus Genome with Other Murine Leukemia Virus Genomes
    Virology, 1997
    Co-Authors: Anis H Khimani, Thomas G Graf, Temple F Smith, Ruth M Ruprecht
    Abstract:

    Abstract We report the complete nucleotide sequence of the genome of Rauscher murine Leukemia Virus (R-MuLV), the replication-competent helper Virus present in the Rauscher Virus complex, and its phylogenetic relationship with other murine Leukemia Virus genomes. An overall sequence identity of 97.6% was found between R-MuLV and the Friend helper Virus (F-MuLV), and the two Viruses were closely related on the phylogenetic trees constructed from either gag, pol, or env sequences. Moloney murine Leukemia Virus (Mo-MuLV) was the next closest relative to R-MuLV and F-MuLV on all trees, followed by Akv and radiation Leukemia Virus (RadLV). The most distantly related helper Virus was Hortulanus murine Leukemia Virus (Ho-MuLV). Interestingly, Cas-Br-E branched with Mo-MuLV on the gag and pol trees, whereas on the env tree, it revealed the highest degree of relatedness to Ho-MuLV, possibly due to an ancient recombination with an Ho-MuLV ancestor. In summary, a phylogenetic analysis involving various MuLVs has been performed, in which the postulated close relationship between R-MuLV and F-MuLV has been confirmed, consistent with the pathobiology of the two Viruses.

Julie Overbaugh - One of the best experts on this subject based on the ideXlab platform.

  • Identification and deletion of sequences required for feline Leukemia Virus RNA packaging and construction of a high-titer feline Leukemia Virus packaging cell line.
    Virology, 1996
    Co-Authors: Cara Carthel Burns, Maria Moser, Jennifer D. Banks, John P. Alderete, Julie Overbaugh
    Abstract:

    Abstract Sequences required for specific encapsidation of feline Leukemia Virus (FeLV) genomic RNA have not yet been defined. Deletion of 107 nucleotides between the splice donor (SD) and thegagcoding region of a prototypic subgroup A FeLV, 61E, resulted in an approximately 200-fold reduction of packaged viral RNA. Virus particle production was not disrupted by the deletion, although viral infectivity was dramatically reduced. These data indicate that the 107-nucleotide sequence comprises a portion of the FeLV packaging signal. FeLV particles expressed from the deleted genome were able to efficiently package murine Leukemia Virus vectors, resulting in high-titer G418RVirus production. This system can be easily adapted to produce FeLV particles that contain envelope proteins from other feline Leukemia Virus subgroups and will be broadly useful for studies of FeLV envelope/receptor interactions.