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A Galante - One of the best experts on this subject based on the ideXlab platform.
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Leukocyte Aggregation in patients with chronic cerebrovascular disease
Acta Neurologica Scandinavica, 2009Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, A GalanteAbstract:It has been previously shown that an increase in Leukocyte Aggregation may represent a risk factor for the development of acute ischemic stroke. Little is known about the role of this parameter in patients with recurrent stroke. For this purpose, we evaluated monthly for two years the level of Leukocyte Aggregation in patients with a first episode of acute ischemic stroke, in order to assess if this factor may predict further cerebral ischemic events. Patients with recurrent stroke had significantly higher values of Leukocyte Aggregation than those free of recurrences. We conclude that the regular evaluation of Leukocyte Aggregation after ischemic stroke may hel in the identification of patients at risk of recurrence.
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Leukocyte count and Aggregation during the evolution of cerebral ischemic injury
Cerebrovascular Diseases, 1998Co-Authors: Mauro Silvestrini, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, Elio Troisi, Laura Franceschelli, Paola Piccolo, A GalanteAbstract:We evaluated Leukocyte Aggregation by means of the leukergy test and count in 26 patients with atherothrombotic stroke and in 10 patients with transient ischemic attacks. The evaluation was performed within 24 h from the onset of symptoms and then repeated on day 2, 4, 6 and 8. Data were compared with those of 10 healthy controls. Stroke patients were followed until day 30 when a clinical examination and brain computed tomography were performed to evaluate the extent and outcome of cerebral damage. Both Leukocyte Aggregation and count were significantly increased in stroke patients with respect to controls. While Leukocyte count was not able to differentiate the severity of neurological impairment in stroke patients, Leukocyte Aggregation was significantly higher in major than in minor stroke patients on days 2 and 4 (p < 0.05). Moreover, while values of Leukocyte count recorded at entry remained substantially stable in the following determinations in all groups, Leukocyte Aggregation showed a significant increase (p < 0.05) on day 4 with respect to all the other determinations in major stroke patients. These findings show that the extent and temporal profile of changes in Leukocyte count and Aggregation are different in patients with cerebrovascular disease and suggest an involvement of altered Leukocyte rheology in the development of cerebral ischemic injury.
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persistent increase of Leukocyte Aggregation in a patient with relapsing ischemic stroke and ulcerative colitis
European Journal of Neurology, 1995Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, A GalanteAbstract:There is an increased incidence of central nervous system thromboembolic events in patients with inflammatory bowel disease. The possible pathogenetic link between the two diseases is not clear. Experimental and clinical observations suggest the possible role of Leukocyte activation in the induction of ischemic and inflammatory events. In a patient with relapsing ischemic stroke and ulcerative colitis, serial determinations of Leukocyte Aggregation, by means of the modified leukergy test, were performed. A persistent increase of the percentage of aggregated Leukocytes with values much higher than those of healthy subjects and patients with ischemic stroke was found during and between the episodes of ischemia and inflammation. The particular temporal pattern of clinical and hemorheological alterations suggests that the persistent activation of Leukocytes may represent a risk factor for the development of both ischemic and inflammatory disease.
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Leukocyte Aggregation in patients with a previous cerebral ischemic event
Stroke, 1994Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, S Carta, A GalanteAbstract:The role of Leukocyte Aggregation in ischemic stroke is controversial. In this study we investigated this hemorheologic alteration in patients at risk for stroke. Leukocyte Aggregation was evaluated with the leukergy test in 61 patients with a recent cerebral ischemic event and in 61 control subjects. In patients Leukocyte Aggregation was significantly higher than in control subjects (3.8 +/- 3.4% versus 2.5 +/- 2.2%; P = .01). In control subjects, the presence of vascular risk factors was associated with values of Aggregation similar to those observed in patients. These findings suggest an association between altered Leukocyte Aggregation and cerebrovascular disease. Further investigations are needed to evaluate whether this hemorheologic alteration can be considered a marker of increased risk for stroke.
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serial determinations of Leukocyte Aggregation in patients with ischemic stroke
International Journal of Neuroscience, 1993Co-Authors: A Galante, Mauro Silvestrini, Andrea Magrini, A Pietroiusti, Roberto Floris, Gme Simonetti, Paolo Stanzione, Giorgio BernardiAbstract:The pattern of Leukocyte Aggregation and its possible relationship with the clinical outcome were evaluated in 35 untreated patients with acute cerebrovascular disease during each of the first three days of hospitalization. Although statistically significant differences were found when comparing patients and age and sex matched controls during any of the 3 days, a large overlapping of values was noted in each comparison. The overlapping was strongly reduced when the average 3-day value was compared. Similarly, when comparing subgroups of patients classified on the basis of their clinical status, the overlap of values observed during any of the 3 days dropped from 70% to 30% when considering the average 3-day levels of Leukocyte Aggregation. Furthermore, the level of correlation between Leukocyte Aggregation and size of cerebral infarction was better when considering the average 3-day value (p = .005) than the best single-day correlation (p = .01). These data suggest that the association between acute cerebrovascular ischemia and Leukocyte rheology is better discovered when repeated evaluation of the latter are performed.
Mauro Silvestrini - One of the best experts on this subject based on the ideXlab platform.
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Leukocyte Aggregation in patients with chronic cerebrovascular disease
Acta Neurologica Scandinavica, 2009Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, A GalanteAbstract:It has been previously shown that an increase in Leukocyte Aggregation may represent a risk factor for the development of acute ischemic stroke. Little is known about the role of this parameter in patients with recurrent stroke. For this purpose, we evaluated monthly for two years the level of Leukocyte Aggregation in patients with a first episode of acute ischemic stroke, in order to assess if this factor may predict further cerebral ischemic events. Patients with recurrent stroke had significantly higher values of Leukocyte Aggregation than those free of recurrences. We conclude that the regular evaluation of Leukocyte Aggregation after ischemic stroke may hel in the identification of patients at risk of recurrence.
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Leukocyte count and Aggregation during the evolution of cerebral ischemic injury
Cerebrovascular Diseases, 1998Co-Authors: Mauro Silvestrini, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, Elio Troisi, Laura Franceschelli, Paola Piccolo, A GalanteAbstract:We evaluated Leukocyte Aggregation by means of the leukergy test and count in 26 patients with atherothrombotic stroke and in 10 patients with transient ischemic attacks. The evaluation was performed within 24 h from the onset of symptoms and then repeated on day 2, 4, 6 and 8. Data were compared with those of 10 healthy controls. Stroke patients were followed until day 30 when a clinical examination and brain computed tomography were performed to evaluate the extent and outcome of cerebral damage. Both Leukocyte Aggregation and count were significantly increased in stroke patients with respect to controls. While Leukocyte count was not able to differentiate the severity of neurological impairment in stroke patients, Leukocyte Aggregation was significantly higher in major than in minor stroke patients on days 2 and 4 (p < 0.05). Moreover, while values of Leukocyte count recorded at entry remained substantially stable in the following determinations in all groups, Leukocyte Aggregation showed a significant increase (p < 0.05) on day 4 with respect to all the other determinations in major stroke patients. These findings show that the extent and temporal profile of changes in Leukocyte count and Aggregation are different in patients with cerebrovascular disease and suggest an involvement of altered Leukocyte rheology in the development of cerebral ischemic injury.
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persistent increase of Leukocyte Aggregation in a patient with relapsing ischemic stroke and ulcerative colitis
European Journal of Neurology, 1995Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, A GalanteAbstract:There is an increased incidence of central nervous system thromboembolic events in patients with inflammatory bowel disease. The possible pathogenetic link between the two diseases is not clear. Experimental and clinical observations suggest the possible role of Leukocyte activation in the induction of ischemic and inflammatory events. In a patient with relapsing ischemic stroke and ulcerative colitis, serial determinations of Leukocyte Aggregation, by means of the modified leukergy test, were performed. A persistent increase of the percentage of aggregated Leukocytes with values much higher than those of healthy subjects and patients with ischemic stroke was found during and between the episodes of ischemia and inflammation. The particular temporal pattern of clinical and hemorheological alterations suggests that the persistent activation of Leukocytes may represent a risk factor for the development of both ischemic and inflammatory disease.
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Leukocyte Aggregation in patients with a previous cerebral ischemic event
Stroke, 1994Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, S Carta, A GalanteAbstract:The role of Leukocyte Aggregation in ischemic stroke is controversial. In this study we investigated this hemorheologic alteration in patients at risk for stroke. Leukocyte Aggregation was evaluated with the leukergy test in 61 patients with a recent cerebral ischemic event and in 61 control subjects. In patients Leukocyte Aggregation was significantly higher than in control subjects (3.8 +/- 3.4% versus 2.5 +/- 2.2%; P = .01). In control subjects, the presence of vascular risk factors was associated with values of Aggregation similar to those observed in patients. These findings suggest an association between altered Leukocyte Aggregation and cerebrovascular disease. Further investigations are needed to evaluate whether this hemorheologic alteration can be considered a marker of increased risk for stroke.
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serial determinations of Leukocyte Aggregation in patients with ischemic stroke
International Journal of Neuroscience, 1993Co-Authors: A Galante, Mauro Silvestrini, Andrea Magrini, A Pietroiusti, Roberto Floris, Gme Simonetti, Paolo Stanzione, Giorgio BernardiAbstract:The pattern of Leukocyte Aggregation and its possible relationship with the clinical outcome were evaluated in 35 untreated patients with acute cerebrovascular disease during each of the first three days of hospitalization. Although statistically significant differences were found when comparing patients and age and sex matched controls during any of the 3 days, a large overlapping of values was noted in each comparison. The overlapping was strongly reduced when the average 3-day value was compared. Similarly, when comparing subgroups of patients classified on the basis of their clinical status, the overlap of values observed during any of the 3 days dropped from 70% to 30% when considering the average 3-day levels of Leukocyte Aggregation. Furthermore, the level of correlation between Leukocyte Aggregation and size of cerebral infarction was better when considering the average 3-day value (p = .005) than the best single-day correlation (p = .01). These data suggest that the association between acute cerebrovascular ischemia and Leukocyte rheology is better discovered when repeated evaluation of the latter are performed.
Giorgio Bernardi - One of the best experts on this subject based on the ideXlab platform.
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Leukocyte Aggregation in patients with chronic cerebrovascular disease
Acta Neurologica Scandinavica, 2009Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, A GalanteAbstract:It has been previously shown that an increase in Leukocyte Aggregation may represent a risk factor for the development of acute ischemic stroke. Little is known about the role of this parameter in patients with recurrent stroke. For this purpose, we evaluated monthly for two years the level of Leukocyte Aggregation in patients with a first episode of acute ischemic stroke, in order to assess if this factor may predict further cerebral ischemic events. Patients with recurrent stroke had significantly higher values of Leukocyte Aggregation than those free of recurrences. We conclude that the regular evaluation of Leukocyte Aggregation after ischemic stroke may hel in the identification of patients at risk of recurrence.
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Leukocyte count and Aggregation during the evolution of cerebral ischemic injury
Cerebrovascular Diseases, 1998Co-Authors: Mauro Silvestrini, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, Elio Troisi, Laura Franceschelli, Paola Piccolo, A GalanteAbstract:We evaluated Leukocyte Aggregation by means of the leukergy test and count in 26 patients with atherothrombotic stroke and in 10 patients with transient ischemic attacks. The evaluation was performed within 24 h from the onset of symptoms and then repeated on day 2, 4, 6 and 8. Data were compared with those of 10 healthy controls. Stroke patients were followed until day 30 when a clinical examination and brain computed tomography were performed to evaluate the extent and outcome of cerebral damage. Both Leukocyte Aggregation and count were significantly increased in stroke patients with respect to controls. While Leukocyte count was not able to differentiate the severity of neurological impairment in stroke patients, Leukocyte Aggregation was significantly higher in major than in minor stroke patients on days 2 and 4 (p < 0.05). Moreover, while values of Leukocyte count recorded at entry remained substantially stable in the following determinations in all groups, Leukocyte Aggregation showed a significant increase (p < 0.05) on day 4 with respect to all the other determinations in major stroke patients. These findings show that the extent and temporal profile of changes in Leukocyte count and Aggregation are different in patients with cerebrovascular disease and suggest an involvement of altered Leukocyte rheology in the development of cerebral ischemic injury.
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persistent increase of Leukocyte Aggregation in a patient with relapsing ischemic stroke and ulcerative colitis
European Journal of Neurology, 1995Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, A GalanteAbstract:There is an increased incidence of central nervous system thromboembolic events in patients with inflammatory bowel disease. The possible pathogenetic link between the two diseases is not clear. Experimental and clinical observations suggest the possible role of Leukocyte activation in the induction of ischemic and inflammatory events. In a patient with relapsing ischemic stroke and ulcerative colitis, serial determinations of Leukocyte Aggregation, by means of the modified leukergy test, were performed. A persistent increase of the percentage of aggregated Leukocytes with values much higher than those of healthy subjects and patients with ischemic stroke was found during and between the episodes of ischemia and inflammation. The particular temporal pattern of clinical and hemorheological alterations suggests that the persistent activation of Leukocytes may represent a risk factor for the development of both ischemic and inflammatory disease.
-
Leukocyte Aggregation in patients with a previous cerebral ischemic event
Stroke, 1994Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, S Carta, A GalanteAbstract:The role of Leukocyte Aggregation in ischemic stroke is controversial. In this study we investigated this hemorheologic alteration in patients at risk for stroke. Leukocyte Aggregation was evaluated with the leukergy test in 61 patients with a recent cerebral ischemic event and in 61 control subjects. In patients Leukocyte Aggregation was significantly higher than in control subjects (3.8 +/- 3.4% versus 2.5 +/- 2.2%; P = .01). In control subjects, the presence of vascular risk factors was associated with values of Aggregation similar to those observed in patients. These findings suggest an association between altered Leukocyte Aggregation and cerebrovascular disease. Further investigations are needed to evaluate whether this hemorheologic alteration can be considered a marker of increased risk for stroke.
-
serial determinations of Leukocyte Aggregation in patients with ischemic stroke
International Journal of Neuroscience, 1993Co-Authors: A Galante, Mauro Silvestrini, Andrea Magrini, A Pietroiusti, Roberto Floris, Gme Simonetti, Paolo Stanzione, Giorgio BernardiAbstract:The pattern of Leukocyte Aggregation and its possible relationship with the clinical outcome were evaluated in 35 untreated patients with acute cerebrovascular disease during each of the first three days of hospitalization. Although statistically significant differences were found when comparing patients and age and sex matched controls during any of the 3 days, a large overlapping of values was noted in each comparison. The overlapping was strongly reduced when the average 3-day value was compared. Similarly, when comparing subgroups of patients classified on the basis of their clinical status, the overlap of values observed during any of the 3 days dropped from 70% to 30% when considering the average 3-day levels of Leukocyte Aggregation. Furthermore, the level of correlation between Leukocyte Aggregation and size of cerebral infarction was better when considering the average 3-day value (p = .005) than the best single-day correlation (p = .01). These data suggest that the association between acute cerebrovascular ischemia and Leukocyte rheology is better discovered when repeated evaluation of the latter are performed.
Antonio Pietroiusti - One of the best experts on this subject based on the ideXlab platform.
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Leukocyte Aggregation in patients with chronic cerebrovascular disease
Acta Neurologica Scandinavica, 2009Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, A GalanteAbstract:It has been previously shown that an increase in Leukocyte Aggregation may represent a risk factor for the development of acute ischemic stroke. Little is known about the role of this parameter in patients with recurrent stroke. For this purpose, we evaluated monthly for two years the level of Leukocyte Aggregation in patients with a first episode of acute ischemic stroke, in order to assess if this factor may predict further cerebral ischemic events. Patients with recurrent stroke had significantly higher values of Leukocyte Aggregation than those free of recurrences. We conclude that the regular evaluation of Leukocyte Aggregation after ischemic stroke may hel in the identification of patients at risk of recurrence.
-
Leukocyte count and Aggregation during the evolution of cerebral ischemic injury
Cerebrovascular Diseases, 1998Co-Authors: Mauro Silvestrini, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, Elio Troisi, Laura Franceschelli, Paola Piccolo, A GalanteAbstract:We evaluated Leukocyte Aggregation by means of the leukergy test and count in 26 patients with atherothrombotic stroke and in 10 patients with transient ischemic attacks. The evaluation was performed within 24 h from the onset of symptoms and then repeated on day 2, 4, 6 and 8. Data were compared with those of 10 healthy controls. Stroke patients were followed until day 30 when a clinical examination and brain computed tomography were performed to evaluate the extent and outcome of cerebral damage. Both Leukocyte Aggregation and count were significantly increased in stroke patients with respect to controls. While Leukocyte count was not able to differentiate the severity of neurological impairment in stroke patients, Leukocyte Aggregation was significantly higher in major than in minor stroke patients on days 2 and 4 (p < 0.05). Moreover, while values of Leukocyte count recorded at entry remained substantially stable in the following determinations in all groups, Leukocyte Aggregation showed a significant increase (p < 0.05) on day 4 with respect to all the other determinations in major stroke patients. These findings show that the extent and temporal profile of changes in Leukocyte count and Aggregation are different in patients with cerebrovascular disease and suggest an involvement of altered Leukocyte rheology in the development of cerebral ischemic injury.
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persistent increase of Leukocyte Aggregation in a patient with relapsing ischemic stroke and ulcerative colitis
European Journal of Neurology, 1995Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, A GalanteAbstract:There is an increased incidence of central nervous system thromboembolic events in patients with inflammatory bowel disease. The possible pathogenetic link between the two diseases is not clear. Experimental and clinical observations suggest the possible role of Leukocyte activation in the induction of ischemic and inflammatory events. In a patient with relapsing ischemic stroke and ulcerative colitis, serial determinations of Leukocyte Aggregation, by means of the modified leukergy test, were performed. A persistent increase of the percentage of aggregated Leukocytes with values much higher than those of healthy subjects and patients with ischemic stroke was found during and between the episodes of ischemia and inflammation. The particular temporal pattern of clinical and hemorheological alterations suggests that the persistent activation of Leukocytes may represent a risk factor for the development of both ischemic and inflammatory disease.
-
Leukocyte Aggregation in patients with a previous cerebral ischemic event
Stroke, 1994Co-Authors: Mauro Silvestrini, Maria Matteis, Giorgio Bernardi, Antonio Pietroiusti, Andrea Magrini, S Carta, A GalanteAbstract:The role of Leukocyte Aggregation in ischemic stroke is controversial. In this study we investigated this hemorheologic alteration in patients at risk for stroke. Leukocyte Aggregation was evaluated with the leukergy test in 61 patients with a recent cerebral ischemic event and in 61 control subjects. In patients Leukocyte Aggregation was significantly higher than in control subjects (3.8 +/- 3.4% versus 2.5 +/- 2.2%; P = .01). In control subjects, the presence of vascular risk factors was associated with values of Aggregation similar to those observed in patients. These findings suggest an association between altered Leukocyte Aggregation and cerebrovascular disease. Further investigations are needed to evaluate whether this hemorheologic alteration can be considered a marker of increased risk for stroke.
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influence of acute and chronic smoking on Leukocyte Aggregation
Clinical physiology and biochemistry, 1993Co-Authors: A Galante, Antonio Pietroiusti, Andrea Magrini, S Carta, C Polucci, E Benincasa, B DomeniciAbstract:The aim of the present study was to evaluate whether alterations of Leukocyte Aggregation are present before the development of smoking-associated diseases. Leukocyte Aggregation, studied by means of the leukergy test, was significantly higher in 24 healthy smokers as compared to 18 healthy non-smokers (mean + SE) 4.4 +/- 2.2 vs 1.5 +/- 1.3; P < 0.02. Intermediate values were found in ex-smokers. A significant increase was observed in smokers who underwent the acute smoking of three cigarettes in 20 minutes (mean +/- SE) 3.5 +/- 1.9 vs 7.5 +/- 2.6; P < 0.02. These data indicate that changes of Leukocyte Aggregation induced by smoking are present in healthy subjects before the development of clinically evident diseases associated with smoking.
Günther Deuschl - One of the best experts on this subject based on the ideXlab platform.
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Determinants of Platelet-Leukocyte Aggregation and Platelet Activation in Stroke
Cerebrovascular Diseases, 2015Co-Authors: Barbara Schmalbach, Olga Stepanow, Arne Jochens, Christian Riedel, Günther Deuschl, Gregor KuhlenbäumerAbstract:Background: Platelet-Leukocyte Aggregation (PLA) and platelet activation are found to be on the higher side in ischemic stroke patients. The correlation of PLA with clinical features has not been intensively investigated and the influence of genetic factors on PLA is still unexplored. The interaction of platelets with Leukocytes is mainly determined by the proteins encoded by six genes: P-Selectin (SELP encodes CD62P) on the thrombocyte binding to P-Selectin-Glycoprotein-Ligand-1 (PSGL1) on the Leukocyte, intracellular-adhesion-molecule 2 (ICAM2) interacting with Integrin alpha M (ITGAM) and Glycoprotein 1b-alpha (GP1BA) binding to Integrin alpha L (ITGAL). Methods: Seventy-nine patients with acute ischemic stroke and 151 controls without vascular disease from a single German center were enrolled. A neurologist and a neuroradiologist ascertained clinical and radiological features. PLA and platelet activation were analyzed using flow cytometry with various antibodies. Coding as well as tagging SNPs in six genes determining PLA were genotyped. Three groups of parameters were correlated with each other: (i) clinical and radiological parameters, (ii) laboratory parameters, (iii) genetic parameters. For the comparisons, robust nonparametric statistical tests were applicable. Results: PLA and platelet activation were higher in ischemic stroke patients compared to controls. Both, anticoagulant and antiplatelet treatment in the patient group affected platelet activation but not PLA. PLA correlated weakly with measures of stroke severity but not with thrombus length or stroke etiology. The association of SNP rs2228315 in the P-Selectin Glycoprotein Ligand-1-gene (PSGL1) with ischemic stroke and platelet activation was significant before correction for multiple testing while a trend was observed for the association with PLA. Regression analysis revealed that (i) platelet activation was an independent determinant of stroke, (ii) that PLA correlated with stroke, sex, age and platelet activation and (iii) that platelet activation correlated only with stroke. None of the SNPs survived in the regression analysis for stroke, PLA or platelet activation as dependent variables. Conclusions: The most important result of our study is that PLA and platelet activation are independent of other vascular risk factors correlated with stroke in our sample. In addition, we identified the missense SNP rs2228315 in the PSGL1-gene as a candidate polymorphism for ischemic stroke-related PLA. Association between this SNP and stroke as well as coronary artery disease has also been shown by two other studies.
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platelet Leukocyte interaction and platelet activation in acute stroke with and without preceding infection
Arteriosclerosis Thrombosis and Vascular Biology, 2005Co-Authors: J A Zeller, A Lenz, Christoph Eschenfelder, Peter Zunker, Günther DeuschlAbstract:Objective— Acute coronary syndromes and ischemic cerebral stroke share similarities regarding elevated platelet activation. In coronary syndromes, the importance of inflammation with platelet-Leukocyte interaction has been demonstrated. Recent infection is an established risk factor for ischemic stroke; the role of platelet-Leukocyte interaction in these patients had not been investigated. Methods and Results— Using a flow cytometric assay we investigated 58 stroke patients, 21 with and 37 without infection 1 week before acute cerebral ischemia, and compared them to 58 controls with regard to platelet-Leukocyte Aggregation and platelet activation on admission and on day 7. Patients with previous infection were significantly up-regulated with regard to platelet activation and platelet-Leukocyte Aggregation compared with patients without infection. On day 7, these increases in the postinfective group had drawn level with the lower values of the other patients. As reported previously, recent infection was associated with a more severe postischemic deficit. Conclusions— These results suggest an important role of intercellular platelet-Leukocyte interaction in the pathophysiology of acute cerebral ischemia which may also contribute to the increased incidence and clinical severity of ischemic stroke following infection. This may lead to therapeutic considerations of blocking intercellular adhesion molecules like P-selectin or the P-selectin glycoprotein ligand.
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platelet Leukocyte interaction and platelet activation in migraine a link to ischemic stroke
Journal of Neurology Neurosurgery and Psychiatry, 2004Co-Authors: J A Zeller, K Frahm, R Baron, Robert Stingele, Günther DeuschlAbstract:Objectives: Migraine has been identified as an independent risk factor for ischemic stroke. Both neurogenic inflammation and platelet activation have been linked to the pathophysiology of migraine. Increased platelet activation results in up-regulation of specific binding to Leukocytes which promotes pro-inflammatory Leukocyte secretion and their tethering to endothelium, a mechanism that has been demonstrated in stroke and which could provide a link to migraine. We aimed to determine whether platelet–Leukocyte Aggregation is increased in migraine patients outside an acute attack. Methods: Seventy two patients with migraine according to IHS criteria were compared to a control group (n = 72). Whole blood flow cytometry was used to quantify the activation dependent P selectin on the platelet, and to assess the fraction of platelets bound to the different Leukocyte subsets. Results: Migraine patients showed significantly more platelet–Leukocyte aggregates compared to the control subjects (p = 0.003). This effect was driven by an increased polymorphonuclear cell–platelet Aggregation (p = 0.003) whereas platelet Aggregation with monocytes and lymphocytes was not. Platelet activation was also increased (p = 0.001). Conclusions: In migraine pro-inflammatory platelet adhesion to Leukocytes occurs during the headache free interval similar to that seen in acute coronary and cerebrovascular syndromes. This may suggest a link between migraine and stroke on a cellular level.