The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Erik Berg Schmidt - One of the best experts on this subject based on the ideXlab platform.
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effects of perioperative supplementation with omega 3 fatty acids on Leukotriene b4 and Leukotriene B5 production by stimulated neutrophils in patients with colorectal cancer a randomized placebo controlled intervention trial
Nutrients, 2014Co-Authors: Lone Schmidt Sorensen, Ole Thorlaciusussing, Henrik Hojgaard Rasmussen, Soren Lundbyechristensen, Philip C Calder, Karen Lindorfflarsen, Erik Berg SchmidtAbstract:Omega-3 fatty acids (n-3 FA) may have beneficial clinical and immune-modulating effects in surgical patients. In a randomized, double-blind, prospective, placebo-controlled trial, 148 patients referred for elective colorectal cancer surgery received an n-3 FA-enriched oral nutritional supplement (ONS) providing 2.0 g of eicosapentaenoic acid (EPA) and 1.0 g of docosahexaenoic acid (DHA) per day or a standard ONS for seven days before surgery. On the day of operation, there was a significant increase in the production of Leukotriene B5 (LTB5) (p < 0.01) and 5-hydroxyeicosapentaenoic acid (5-HEPE) (p < 0.01), a significant decrease in the production of Leukotriene B4 (LTB4) (p < 0.01) and a trend for a decrease in the production of 5-hydroxyeicosatetraenoic acid (5-HETE) (p < 0.1) from stimulated neutrophils in the active group compared with controls. There was no association between LTB4 values and postoperative complications. In conclusion, oral n-3 FA exerts anti-inflammatory effects in surgical patients, without reducing the risk of postoperative complications.
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the effect of n 3 polyunsaturated fatty acids on Leukotriene b4 and Leukotriene B5 production from stimulated neutrophil granulocytes in patients with chronic kidney disease
Prostaglandins Leukotrienes and Essential Fatty Acids, 2011Co-Authors: T Maaloe, Erik Berg Schmidt, My Svensson, Inge Valbak Aardestrup, Jeppe Hagstrup ChristensenAbstract:Abstract The proinflammatory Leukotriene B 4 (LTB 4 ) may be of importance in the progression of chronic kidney disease (CKD). We investigated whether n-3 polyunsaturated fatty acids (PUFA) decrease LTB 4 and increase the formation of the less inflammatory Leukotriene B 5 (LTB 5 ) in patients with CKD. Fifty-six patients with CKD stage 2–5 were randomised to 2.4g n-3 PUFA or olive oil for 8 weeks. Compared to controls, n-3 PUFA significantly decreased release of LTB 4 ( p p 5 ( p p 4 and 5-HETE and significantly increased LTB 5 and 5-HEPE. No effect was seen on kidney function.
C A Herman - One of the best experts on this subject based on the ideXlab platform.
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Leukotriene b4 and Leukotriene B5 have binding sites on lung membranes and cause contraction of bullfrog lung
Journal of Pharmacology and Experimental Therapeutics, 1992Co-Authors: J J Andazola, J A Underwood, M Chiono, O A Torres, C A HermanAbstract:Leukotriene (LT)B4 and LTB5 cause contraction of isolated bullfrog lung. LTB4 receptors were characterized in membranes prepared from bullfrog lung. Binding of [3H]LTB4 was maximal at 5 min and was reversible with the addition of 1000-fold excess LTB4. Scatchard analysis indicated a single class of binding sites with a Kd of 2.22 nM and a Bmax of 1228.86 fmol/mg protein. The Kd and the Bmax values in the presence of guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S) were 2.76 nM and 1289.61 fmol/mg protein, respectively. The Ki values for LTB4, LTB5 and 20(OH)-LTB4 were 5.5, 30.5 and 144.0 nM, respectively, whereas 20(COOH)-LTB4 was ineffective in preventing binding of [3H] LTB4 from 10(-9) to 10(-5) M. The peptide Leukotrienes LTC4, LTD4 and LTE4 failed to inhibit the specific binding of [3H]LTB4. GTP gamma S in concentrations from 10(-10) to 10(-4) M did not affect the binding of 5 nM [3H]LTB4. Neither the mammalian LTD4 antagonist LY171883 nor the mammalian LTB4 antagonist LY255283 was an effective competitor for the bullfrog lung LTB4 receptor. In addition, sulfhydryl-modifying reagents NEM and PCMP did not affect LTB4 binding as they do in mammalian membrane preparations. The LTB4 receptor shows some differences from the described mammalian receptor. The cell type containing the LTB4 receptor remains to be determined.
Wei-ming Kang - One of the best experts on this subject based on the ideXlab platform.
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superiority of a fish oil enriched emulsion to medium chain triacylglycerols long chain triacylglycerols in gastrointestinal surgery patients a randomized clinical trial
Nutrition, 2012Co-Authors: Jin Wang, Wei-ming KangAbstract:Abstract Objective Compared with soybean oil, a fish oil–enriched emulsion can improve the clinical outcomes of patients requiring parenteral nutrition. However, the superiority of fish oil emulsion to medium-chain triacylglycerols/long-chain triacylglycerols for short-term administration has seldom been discussed. Methods Sixty-four adult patients with gastrointestinal diseases were randomly assigned to receive isocaloric and isonitrogenous total parenteral nutrition with an ω-3 fatty acid–enriched emulsion (Lipoplus; study group, n = 32) or medium-chain triacylglycerols/long-chain triacylglycerols (Lipofundin; control group, n = 32) for 5 d after surgery. Safety and efficacy parameters were assessed on postoperative days 1, 3, and 6. Results Clinical outcomes including infectious complications and systemic inflammatory response syndrome were comparable between the two groups. Total bilirubin decreased over time in the study group versus an increase in the control group ( P = 0.017). Activated partial thromboplastin time in the study group was prolonged significantly compared with the control group from days 1 to 3 ( P = 0.002), although the prolongation stopped at the study termination. There were no differences in changes of C-reactive protein, interleukin (IL)-1, IL-8, IL-10, vascular endothelial growth factor (VEGF), and the distribution of the T-cell subpopulation between the two groups. However, fish oil consumption led to an increase in Leukotriene B5/ Leukotriene B4 and significant decreases in IL-6, tumor necrosis factor-α, and nuclear factor-κB. Furthermore, the overall changes in tumor necrosis factor-α and nuclear factor-κB were positively associated ( R 2 = 0.295, P Conclusions Gastrointestinal surgery patients benefited from a fish oil–enriched emulsion rather than medium-chain triacylglycerols/long-chain triacylglycerols in the amelioration of liver function and immune status. The positive association of tumor necrosis factor-α and nuclear factor-κB might be involved in the potential anti-inflammation mechanism of fish oil.
J J Andazola - One of the best experts on this subject based on the ideXlab platform.
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Leukotriene b4 and Leukotriene B5 have binding sites on lung membranes and cause contraction of bullfrog lung
Journal of Pharmacology and Experimental Therapeutics, 1992Co-Authors: J J Andazola, J A Underwood, M Chiono, O A Torres, C A HermanAbstract:Leukotriene (LT)B4 and LTB5 cause contraction of isolated bullfrog lung. LTB4 receptors were characterized in membranes prepared from bullfrog lung. Binding of [3H]LTB4 was maximal at 5 min and was reversible with the addition of 1000-fold excess LTB4. Scatchard analysis indicated a single class of binding sites with a Kd of 2.22 nM and a Bmax of 1228.86 fmol/mg protein. The Kd and the Bmax values in the presence of guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S) were 2.76 nM and 1289.61 fmol/mg protein, respectively. The Ki values for LTB4, LTB5 and 20(OH)-LTB4 were 5.5, 30.5 and 144.0 nM, respectively, whereas 20(COOH)-LTB4 was ineffective in preventing binding of [3H] LTB4 from 10(-9) to 10(-5) M. The peptide Leukotrienes LTC4, LTD4 and LTE4 failed to inhibit the specific binding of [3H]LTB4. GTP gamma S in concentrations from 10(-10) to 10(-4) M did not affect the binding of 5 nM [3H]LTB4. Neither the mammalian LTD4 antagonist LY171883 nor the mammalian LTB4 antagonist LY255283 was an effective competitor for the bullfrog lung LTB4 receptor. In addition, sulfhydryl-modifying reagents NEM and PCMP did not affect LTB4 binding as they do in mammalian membrane preparations. The LTB4 receptor shows some differences from the described mammalian receptor. The cell type containing the LTB4 receptor remains to be determined.
Jeppe Hagstrup Christensen - One of the best experts on this subject based on the ideXlab platform.
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the effect of n 3 polyunsaturated fatty acids on Leukotriene b4 and Leukotriene B5 production from stimulated neutrophil granulocytes in patients with chronic kidney disease
Prostaglandins Leukotrienes and Essential Fatty Acids, 2011Co-Authors: T Maaloe, Erik Berg Schmidt, My Svensson, Inge Valbak Aardestrup, Jeppe Hagstrup ChristensenAbstract:Abstract The proinflammatory Leukotriene B 4 (LTB 4 ) may be of importance in the progression of chronic kidney disease (CKD). We investigated whether n-3 polyunsaturated fatty acids (PUFA) decrease LTB 4 and increase the formation of the less inflammatory Leukotriene B 5 (LTB 5 ) in patients with CKD. Fifty-six patients with CKD stage 2–5 were randomised to 2.4g n-3 PUFA or olive oil for 8 weeks. Compared to controls, n-3 PUFA significantly decreased release of LTB 4 ( p p 5 ( p p 4 and 5-HETE and significantly increased LTB 5 and 5-HEPE. No effect was seen on kidney function.