The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
Mohd Zubir Bin Matjafri - One of the best experts on this subject based on the ideXlab platform.
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Turbidimeter design and analysis: A review on optical fiber sensors for the Measurement of water turbidity
Sensors, 2009Co-Authors: Ahmad Fairuz Bin Omar, Mohd Zubir Bin MatjafriAbstract:Turbidimeters operate based on the optical phenomena that occur when incident Light through water body is scattered by the existence of foreign particles which are suspended within it. This review paper elaborates on the standards and factors that may influence the Measurement of turbidity. The discussion also focuses on the optical fiber sensor technologies that have been applied within the lab and field environment and have been implemented in the Measurement of water turbidity and concentration of particles. This paper also discusses and compares results from three different turbidimeter designs that use various optical components. Mohd Zubir and Bashah and Daraigan have introduced a design which has simple configurations. Omar and MatJafri, on the other hand, have established a new turbidimeter design that makes use of optical fiber cable as the Light transferring medium. The application of fiber optic cable to the turbidimeter will present a flexible Measurement technique, allowing Measurements to be made online. Scattered Light Measurement through optical fiber cable requires a highly sensitive detector to interpret the scattered Light signal. This has made the optical fiber system have higher sensitivity in measuring turbidity compared to the other two simple turbidimeters presented in this paper. Fiber optic sensors provide the potential for increased sensitivity over large concentration ranges. However, many challenges must be examined to develop sensors that can collect reliable turbidity Measurements in situ.
Richard H Ebright - One of the best experts on this subject based on the ideXlab platform.
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fluorescence anisotropy rapid quantitative assay for protein dna and protein protein interaction
Methods in Enzymology, 1996Co-Authors: Tomasz Heyduk, Hong Tang, Richard H EbrightAbstract:Publisher Summary This chapter discusses fluorescence anisotropy. The quantitative assay for protein-DNA and protein-protein interaction is discussed. Fluorescence spectroscopy involves excitation of a fluorochrome with Light of a wavelength at which the fluorochrome exhibits significant absorption, creating fluorochrome excited state that can be followed by the detection of emitted Light. Measurement of fluorescence polarization involves excitation of a sample with polarized Light and determination of the extent of polarization of the emitted Light. Fluorescence anisotropy assays can be performed using any standard spectrofluorometer equipped with polarizers or using a dedicated fluorescence polarization instrument. Standard spectrofluorometers permit use of a range of excitation and emission wavelengths, and thus a range of different fluorochromes, and are compatible with sample volumes from -0.05 to 4.0 ml. Fluorescence anisotropy analysis of protein-protein interaction requires careful experimental design.
Thomas J T P Van Den Berg - One of the best experts on this subject based on the ideXlab platform.
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reliability of the compensation comparison stray Light Measurement method
Journal of Biomedical Optics, 2006Co-Authors: Joris E Coppens, Luuk Franssen, L J Van Rijn, Thomas J T P Van Den BergAbstract:The compensation comparison (CC) method is a psychophysical technique to measure retinal stray Light. It uses a two alternative forced choice (2AFC) Measurement paradigm. The 25 binary (0 and 1) responses resulting from the 2AFC test are analyzed using maximum likelihood estimates. The likelihood function is used to give two quantities: the most likely stray-Light level of the eye under investigation, and the accuracy of this estimate [called expected standard deviation (ESD)]. The CC method is used in 2422 subjects of the GLARE study. Each eye is tested twice to allow analysis of Measurement repeatability. Furthermore, the large amount of responses is used to evaluate the shape of the psychometric function, for which a mathematical model is used. The shape of the psychometric function found by averaging the 0 and 1 responses fit well to the model function. Data sorted according to ESD show differences in the shape of the psychometric function between good and bad observers. These different shapes for the psychometric function are used to reanalyze the data, but the stray-Light results remain virtually identical. ESD proves to be an efficient tool to detect unreliable Measurements. In clinical practice, ESD may be used to decide whether to repeat a Measurement.
P K Gupta - One of the best experts on this subject based on the ideXlab platform.
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depth resolved fluorescence Measurement in a layered turbid medium by polarized fluorescence spectroscopy
Optics Letters, 2005Co-Authors: Nirmalya Ghosh, Shovan K Majumder, H S Patel, P K GuptaAbstract:We show that, when a turbid medium with a layered fluorophore distribution is excited by linearly polarized Light, Measurement of angle-resolved polarized fluorescence can provide depth-resolved fluorescence Measurements.
Ahmad Fairuz Bin Omar - One of the best experts on this subject based on the ideXlab platform.
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Turbidimeter design and analysis: A review on optical fiber sensors for the Measurement of water turbidity
Sensors, 2009Co-Authors: Ahmad Fairuz Bin Omar, Mohd Zubir Bin MatjafriAbstract:Turbidimeters operate based on the optical phenomena that occur when incident Light through water body is scattered by the existence of foreign particles which are suspended within it. This review paper elaborates on the standards and factors that may influence the Measurement of turbidity. The discussion also focuses on the optical fiber sensor technologies that have been applied within the lab and field environment and have been implemented in the Measurement of water turbidity and concentration of particles. This paper also discusses and compares results from three different turbidimeter designs that use various optical components. Mohd Zubir and Bashah and Daraigan have introduced a design which has simple configurations. Omar and MatJafri, on the other hand, have established a new turbidimeter design that makes use of optical fiber cable as the Light transferring medium. The application of fiber optic cable to the turbidimeter will present a flexible Measurement technique, allowing Measurements to be made online. Scattered Light Measurement through optical fiber cable requires a highly sensitive detector to interpret the scattered Light signal. This has made the optical fiber system have higher sensitivity in measuring turbidity compared to the other two simple turbidimeters presented in this paper. Fiber optic sensors provide the potential for increased sensitivity over large concentration ranges. However, many challenges must be examined to develop sensors that can collect reliable turbidity Measurements in situ.