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Erik Remaut - One of the best experts on this subject based on the ideXlab platform.

  • the expression of the photinus pyralis luciferase gene in staphylococcus aureus cowan i allows the development of a live amplifiable tool for immunodetection
    Applied and Environmental Microbiology, 1996
    Co-Authors: Lothar Steidler, W Yu, Walter Fiers, Erik Remaut
    Abstract:

    We expressed the luc gene, encoding luciferase from Photinus pyralis, in Staphylococcus aureus Cowan I downstream of the plasmid-borne promoter for protein A. Constitutive luciferase synthesis did not impair the growth rate of the host nor did it affect the stability of the plasmid. Light Production started immediately after addition of luciferin. The kinetic profile is of the glowing rather than the peak type. Because S. aureus Cowan I produces large quantities of protein A, of which a substantial part becomes covalently attached to rigid cell walls, the bacterial cells could be specifically immobilized on a substrate to which immunoglobulin G molecules were adsorbed either directly or as secondary antibodies. Light Production from these cells can be used as a reporter tool for the detection of antigen-antibody complexes. Fourfold amplifications of the emitted signals were obtained by in situ incubation of the bound cells in bacterial growth medium.

  • The expression of the Photinus pyralis luciferase gene in Staphylococcus aureus Cowan I allows the development of a live amplifiable tool for immunodetection.
    Applied and Environmental Microbiology, 1996
    Co-Authors: Lothar Steidler, W Yu, Walter Fiers, Erik Remaut
    Abstract:

    We expressed the luc gene, encoding luciferase from Photinus pyralis ,i nStaphylococcus aureus Cowan I downstream of the plasmid-borne promoter for protein A. Constitutive luciferase synthesis did not impair the growth rate of the host nor did it affect the stability of the plasmid. Light Production started immediately after addition of luciferin. The kinetic profile is of the glowing rather than the peak type. BecauseS. aureusCowan I produces large quantities of protein A, of which a substantial part becomes covalently attached to rigid cell walls,thebacterialcellscouldbespecificallyimmobilizedonasubstratetowhichimmunoglobulinGmolecules were adsorbed either directly or as secondary antibodies. Light Production from these cells can be used as a reporter tool for the detection of antigen-antibody complexes. Fourfold amplifications of the emitted signals were obtained by in situ incubation of the bound cells in bacterial growth medium. One of the more striking features of most clinical isolates of Staphylococcus aureusis the presence in the cell walls of large amounts of protein A, a protein which is able to bind certain isotypes of immunoglobulin G (IgG) molecules (9). The Ig bindingcapacityresideswithintheamino-terminalpartofproteinA,whichconsistsoffivehomologousdomainsandextends intothegrowthmedium.Thecarboxy-terminalpartsofprotein Amoleculesareboundcovalentlytothepeptidoglycanlayerof

Lothar Steidler - One of the best experts on this subject based on the ideXlab platform.

  • the expression of the photinus pyralis luciferase gene in staphylococcus aureus cowan i allows the development of a live amplifiable tool for immunodetection
    Applied and Environmental Microbiology, 1996
    Co-Authors: Lothar Steidler, W Yu, Walter Fiers, Erik Remaut
    Abstract:

    We expressed the luc gene, encoding luciferase from Photinus pyralis, in Staphylococcus aureus Cowan I downstream of the plasmid-borne promoter for protein A. Constitutive luciferase synthesis did not impair the growth rate of the host nor did it affect the stability of the plasmid. Light Production started immediately after addition of luciferin. The kinetic profile is of the glowing rather than the peak type. Because S. aureus Cowan I produces large quantities of protein A, of which a substantial part becomes covalently attached to rigid cell walls, the bacterial cells could be specifically immobilized on a substrate to which immunoglobulin G molecules were adsorbed either directly or as secondary antibodies. Light Production from these cells can be used as a reporter tool for the detection of antigen-antibody complexes. Fourfold amplifications of the emitted signals were obtained by in situ incubation of the bound cells in bacterial growth medium.

  • The expression of the Photinus pyralis luciferase gene in Staphylococcus aureus Cowan I allows the development of a live amplifiable tool for immunodetection.
    Applied and Environmental Microbiology, 1996
    Co-Authors: Lothar Steidler, W Yu, Walter Fiers, Erik Remaut
    Abstract:

    We expressed the luc gene, encoding luciferase from Photinus pyralis ,i nStaphylococcus aureus Cowan I downstream of the plasmid-borne promoter for protein A. Constitutive luciferase synthesis did not impair the growth rate of the host nor did it affect the stability of the plasmid. Light Production started immediately after addition of luciferin. The kinetic profile is of the glowing rather than the peak type. BecauseS. aureusCowan I produces large quantities of protein A, of which a substantial part becomes covalently attached to rigid cell walls,thebacterialcellscouldbespecificallyimmobilizedonasubstratetowhichimmunoglobulinGmolecules were adsorbed either directly or as secondary antibodies. Light Production from these cells can be used as a reporter tool for the detection of antigen-antibody complexes. Fourfold amplifications of the emitted signals were obtained by in situ incubation of the bound cells in bacterial growth medium. One of the more striking features of most clinical isolates of Staphylococcus aureusis the presence in the cell walls of large amounts of protein A, a protein which is able to bind certain isotypes of immunoglobulin G (IgG) molecules (9). The Ig bindingcapacityresideswithintheamino-terminalpartofproteinA,whichconsistsoffivehomologousdomainsandextends intothegrowthmedium.Thecarboxy-terminalpartsofprotein Amoleculesareboundcovalentlytothepeptidoglycanlayerof

W Yu - One of the best experts on this subject based on the ideXlab platform.

  • the expression of the photinus pyralis luciferase gene in staphylococcus aureus cowan i allows the development of a live amplifiable tool for immunodetection
    Applied and Environmental Microbiology, 1996
    Co-Authors: Lothar Steidler, W Yu, Walter Fiers, Erik Remaut
    Abstract:

    We expressed the luc gene, encoding luciferase from Photinus pyralis, in Staphylococcus aureus Cowan I downstream of the plasmid-borne promoter for protein A. Constitutive luciferase synthesis did not impair the growth rate of the host nor did it affect the stability of the plasmid. Light Production started immediately after addition of luciferin. The kinetic profile is of the glowing rather than the peak type. Because S. aureus Cowan I produces large quantities of protein A, of which a substantial part becomes covalently attached to rigid cell walls, the bacterial cells could be specifically immobilized on a substrate to which immunoglobulin G molecules were adsorbed either directly or as secondary antibodies. Light Production from these cells can be used as a reporter tool for the detection of antigen-antibody complexes. Fourfold amplifications of the emitted signals were obtained by in situ incubation of the bound cells in bacterial growth medium.

  • The expression of the Photinus pyralis luciferase gene in Staphylococcus aureus Cowan I allows the development of a live amplifiable tool for immunodetection.
    Applied and Environmental Microbiology, 1996
    Co-Authors: Lothar Steidler, W Yu, Walter Fiers, Erik Remaut
    Abstract:

    We expressed the luc gene, encoding luciferase from Photinus pyralis ,i nStaphylococcus aureus Cowan I downstream of the plasmid-borne promoter for protein A. Constitutive luciferase synthesis did not impair the growth rate of the host nor did it affect the stability of the plasmid. Light Production started immediately after addition of luciferin. The kinetic profile is of the glowing rather than the peak type. BecauseS. aureusCowan I produces large quantities of protein A, of which a substantial part becomes covalently attached to rigid cell walls,thebacterialcellscouldbespecificallyimmobilizedonasubstratetowhichimmunoglobulinGmolecules were adsorbed either directly or as secondary antibodies. Light Production from these cells can be used as a reporter tool for the detection of antigen-antibody complexes. Fourfold amplifications of the emitted signals were obtained by in situ incubation of the bound cells in bacterial growth medium. One of the more striking features of most clinical isolates of Staphylococcus aureusis the presence in the cell walls of large amounts of protein A, a protein which is able to bind certain isotypes of immunoglobulin G (IgG) molecules (9). The Ig bindingcapacityresideswithintheamino-terminalpartofproteinA,whichconsistsoffivehomologousdomainsandextends intothegrowthmedium.Thecarboxy-terminalpartsofprotein Amoleculesareboundcovalentlytothepeptidoglycanlayerof

Walter Fiers - One of the best experts on this subject based on the ideXlab platform.

  • the expression of the photinus pyralis luciferase gene in staphylococcus aureus cowan i allows the development of a live amplifiable tool for immunodetection
    Applied and Environmental Microbiology, 1996
    Co-Authors: Lothar Steidler, W Yu, Walter Fiers, Erik Remaut
    Abstract:

    We expressed the luc gene, encoding luciferase from Photinus pyralis, in Staphylococcus aureus Cowan I downstream of the plasmid-borne promoter for protein A. Constitutive luciferase synthesis did not impair the growth rate of the host nor did it affect the stability of the plasmid. Light Production started immediately after addition of luciferin. The kinetic profile is of the glowing rather than the peak type. Because S. aureus Cowan I produces large quantities of protein A, of which a substantial part becomes covalently attached to rigid cell walls, the bacterial cells could be specifically immobilized on a substrate to which immunoglobulin G molecules were adsorbed either directly or as secondary antibodies. Light Production from these cells can be used as a reporter tool for the detection of antigen-antibody complexes. Fourfold amplifications of the emitted signals were obtained by in situ incubation of the bound cells in bacterial growth medium.

  • The expression of the Photinus pyralis luciferase gene in Staphylococcus aureus Cowan I allows the development of a live amplifiable tool for immunodetection.
    Applied and Environmental Microbiology, 1996
    Co-Authors: Lothar Steidler, W Yu, Walter Fiers, Erik Remaut
    Abstract:

    We expressed the luc gene, encoding luciferase from Photinus pyralis ,i nStaphylococcus aureus Cowan I downstream of the plasmid-borne promoter for protein A. Constitutive luciferase synthesis did not impair the growth rate of the host nor did it affect the stability of the plasmid. Light Production started immediately after addition of luciferin. The kinetic profile is of the glowing rather than the peak type. BecauseS. aureusCowan I produces large quantities of protein A, of which a substantial part becomes covalently attached to rigid cell walls,thebacterialcellscouldbespecificallyimmobilizedonasubstratetowhichimmunoglobulinGmolecules were adsorbed either directly or as secondary antibodies. Light Production from these cells can be used as a reporter tool for the detection of antigen-antibody complexes. Fourfold amplifications of the emitted signals were obtained by in situ incubation of the bound cells in bacterial growth medium. One of the more striking features of most clinical isolates of Staphylococcus aureusis the presence in the cell walls of large amounts of protein A, a protein which is able to bind certain isotypes of immunoglobulin G (IgG) molecules (9). The Ig bindingcapacityresideswithintheamino-terminalpartofproteinA,whichconsistsoffivehomologousdomainsandextends intothegrowthmedium.Thecarboxy-terminalpartsofprotein Amoleculesareboundcovalentlytothepeptidoglycanlayerof

Elizabeth M. Boon - One of the best experts on this subject based on the ideXlab platform.

  • A Nitric Oxide-Responsive Quorum Sensing Circuit in Vibrio harveyi Regulates Flagella Production and Biofilm Formation
    International Journal of Molecular Sciences, 2013
    Co-Authors: Bernadette M. Henares, Elizabeth M. Boon
    Abstract:

    Cell signaling plays an important role in the survival of bacterial colonies. They use small molecules to coordinate gene expression in a cell density dependent manner. This process, known as quorum sensing, helps bacteria regulate diverse functions such as bioluminescence, biofilm formation and virulence. In Vibrio harveyi, a bioluminescent marine bacterium, four parallel quorum-sensing systems have been identified to regulate Light Production. We have previously reported that nitric oxide (NO), through the H-NOX/HqsK quorum sensing pathway contributes to Light Production in V. harveyi through the LuxU/LuxO/LuxR quorum sensing pathway. In this study, we show that nitric oxide (NO) also regulates flagellar Production and enhances biofilm formation. Our data suggest that V. harveyi is capable of switching between lifestyles to be able to adapt to changes in the environment.