The Experts below are selected from a list of 51837 Experts worldwide ranked by ideXlab platform
Xing Wang Deng - One of the best experts on this subject based on the ideXlab platform.
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b box containing proteins bbx30 and bbx31 acting downstream of hy5 negatively regulate photomorphogenesis in arabidopsis
Plant Physiology, 2019Co-Authors: Xing Wang Deng, Dongqing Xu, Yan Jiang, Yueqin Heng, Mingquan Ding, Hua Zhou, Xianhai ZhaoAbstract:Light-mediated seedling development is coordinately controlled by a variety of key regulators. Here, we identified two B-box (BBX)-containing proteins, BBX30 and BBX31, as repressors of photomorphogenesis. ELONGATED HYPOCOTYL5, a central regulator of Light Signaling, directly binds to the G-box cis-element present in the promoters of BBX30 and BBX31 and negatively controls their transcription levels in the Light. Seedlings with mutations in BBX30 or BBX31 are hypersensitive to Light, whereas the overexpression of BBX30 or BBX31 leads to hypo-photomorphogenic growth in the Light. Furthermore, transgenic and phenotypic analysis revealed that the B-box domain of BBX30 or BBX31 is essential for their respective functioning in the regulation of photomorphogenic development in plants. In conclusion, BBX30 and BBX31 act as key negative regulators of Light Signaling, and their transcription is repressed by ELONGATED HYPOCOTYL5 through directly associating with their promoters.
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arabidopsis fhy3 and far1 regulate Light induced myo inositol biosynthesis and oxidative stress responses by transcriptional activation of mips1
Molecular Plant, 2016Co-Authors: Tian Tian, Xing Wang Deng, Rongcheng Lin, Haiyang WangAbstract:myo-Inositol-1-phosphate synthase (MIPS) catalyzes the limiting step of inositol biosynthesis and has crucial roles in plant growth and development. In response to stress, the transcription of MIPS1 is induced and the biosynthesis of inositol or inositol derivatives is promoted by unknown mechanisms. Here, we found that the Light Signaling protein FAR-RED ELONGATED HYPOCOTYL3 (FHY3) and its homolog FAR-RED IMPAIRED RESPONSE1 (FAR1) regulate Light-induced inositol biosynthesis and oxidative stress responses by activating the transcription of MIPS1. Disruption of FHY3 and FAR1 caused Light-induced cell death after dark-Light transition, precocious leaf senescence, and increased sensitivity to oxidative stress. Reduction of salicylic acid (SA) accumulation by overexpression of SALICYLIC ACID 3-HYDROXYLASE largely suppressed the cell death phenotype of fhy3 far1 mutant plants, suggesting that FHY3- and FAR1-mediated cell death is dependent on SA. Furthermore, comparative analysis of chromatin immunoprecipitation sequencing and microarray results revealed that FHY3 and FAR1 directly target both MIPS1 and MIPS2. The fhy3 far1 mutant plants showed severely decreased MIPS1/2 transcript levels and reduced inositol levels. Conversely, constitutive expression of MIPS1 partially rescued the inositol contents, caused reduced transcript levels of SA-biosynthesis genes, and prevented oxidative stress in fhy3 far1. Taken together, our results indicate that the Light Signaling proteins FHY3 and FAR1 directly bind the promoter of MIPS1 to activate its expression and thereby promote inositol biosynthesis to prevent Light-induced oxidative stress and SA-dependent cell death.
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beyond repression of photomorphogenesis role switching of cop det fus in Light Signaling
Current Opinion in Plant Biology, 2014Co-Authors: Xi Huang, Xinhao Ouyang, Xing Wang DengAbstract:Light is a pivotal environmental stimulus that promotes plant photomorphogenesis. Substantial progress has been achieved in defining the central repressors of photomorphogenesis, the CONSTITUTIVE PHOTOMORPHOGENIC / DE-ETIOLATED / FUSCA ( COP / DET / FUS ) loci, in the past 20 years. COP/DET/FUS proteins are well-conserved, and regulate a variety of biological processes in plants and animals. The fact that these proteins contribute to the repression of plant photomorphogenesis by regulating the ubiquitin-proteasome-dependent pathway has been well established. Recently, molecular insight has been gained into the functional diversity of COP/DET/FUS. Here, we review the current research on the roles of COP/DET/FUS, with a focus on the functional conversion of COP1 in photomorphogenesis.
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beyond repression of photomorphogenesis role switching of cop det fus in Light Signaling
Current Opinion in Plant Biology, 2014Co-Authors: Xi Huang, Xinhao Ouyang, Xing Wang DengAbstract:Light is a pivotal environmental stimulus that promotes plant photomorphogenesis. Substantial progress has been achieved in defining the central repressors of photomorphogenesis, the CONSTITUTIVE PHOTOMORPHOGENIC / DE-ETIOLATED / FUSCA ( COP / DET / FUS ) loci, in the past 20 years. COP/DET/FUS proteins are well-conserved, and regulate a variety of biological processes in plants and animals. The fact that these proteins contribute to the repression of plant photomorphogenesis by regulating the ubiquitin-proteasome-dependent pathway has been well established. Recently, molecular insight has been gained into the functional diversity of COP/DET/FUS. Here, we review the current research on the roles of COP/DET/FUS, with a focus on the functional conversion of COP1 in photomorphogenesis.
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arabidopsis cop1 spa1 complex and fhy1 fhy3 associate with distinct phosphorylated forms of phytochrome a in balancing Light Signaling
Molecular Cell, 2008Co-Authors: Yusuke Saijo, Yunping Shen, Vicente Rubio, Ute Hoecker, Haiyang Wang, Zhenzhen Zhou, Danmeng Zhu, Xing Wang DengAbstract:Fine tuning of Light Signaling is crucial to plant development. Following Light-triggered nuclear translocation, the photoreceptor phytochrome A (phyA) regulates gene expression under continuous far-red Light and is rapidly destabilized upon red Light irradiation by E3 ubiquitin ligases, including COP1. Here we provide evidence that the Light Signaling repressors SPA proteins contribute to COP1-mediated phyA degradation and that a COP1/SPA1 protein complex is tightly associated with phyA ubiquitination activity. Furthermore, a phosphorylated phyA form accumulates in the nucleus and preferentially associates with the COP1/SPA1 complex. In contrast, underphosphorylated phyA predominantly associates with the phyA-Signaling intermediates FHY3 and FHY1. However, COP1 associates with underphosphorylated phyA in the absence of FHY3 or FHY1, suggesting that phyA associations with FHY3 and FHY1 protect underphosphorylated phyA from being recognized by the COP1/SPA complex. We propose that Light-induced phyA phosphorylation acts as a switch controlling differential interactions of the photoreceptor with signal propagation or attenuation machineries.
Detlef Weigel - One of the best experts on this subject based on the ideXlab platform.
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spf45 related splicing factor for phytochrome Signaling promotes photomorphogenesis by regulating pre mrna splicing in arabidopsis
Proceedings of the National Academy of Sciences of the United States of America, 2017Co-Authors: Patrice A Salome, Estefania Mancini, Carine M Marshall, Frank G Harmon, Marcelo J Yanovsky, Detlef WeigelAbstract:Abstract Light signals regulate plant growth and development by controlling a plethora of gene expression changes. Posttranscriptional regulation, especially pre-mRNA processing, is a key modulator of gene expression; however, the molecular mechanisms linking pre-mRNA processing and Light Signaling are not well understood. Here we report a protein related to the human splicing factor 45 (SPF45) named splicing factor for phytochrome Signaling (SFPS), which directly interacts with the photoreceptor phytochrome B (phyB). In response to Light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP in photobodies. sfps loss-of-function plants are hyposensitive to red, far-red, and blue Light, and flower precociously. SFPS colocalizes with U2 small nuclear ribonucleoprotein-associated factors including U2AF65B, U2A′, and U2AF35A in nuclear speckles, suggesting SFPS might be involved in the 3′ splice site determination. SFPS regulates pre-mRNA splicing of a large number of genes, of which many are involved in regulating Light Signaling, photosynthesis, and the circadian clock under both dark and Light conditions. In vivo RNA immunoprecipitation (RIP) assays revealed that SFPS associates with EARLY FLOWERING 3 (ELF3) mRNA, a critical link between Light Signaling and the circadian clock. Moreover, PHYTOCHROME INTERACTING FACTORS (PIFs) transcription factor genes act downstream of SFPS, as the quadruple pif mutant pifq suppresses defects of sfps mutants. Taken together, these data strongly suggest SFPS modulates Light-regulated developmental processes by controlling pre-mRNA splicing of Light Signaling and circadian clock genes.
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spf45 related splicing factor for phytochrome Signaling promotes photomorphogenesis by regulating pre mrna splicing in arabidopsis
Proceedings of the National Academy of Sciences of the United States of America, 2017Co-Authors: Patrice A Salome, Estefania Mancini, Carine M Marshall, Frank G Harmon, Marcelo J Yanovsky, Detlef Weigel, Ruijiao Xin, Ling ZhuAbstract:Abstract Light signals regulate plant growth and development by controlling a plethora of gene expression changes. Posttranscriptional regulation, especially pre-mRNA processing, is a key modulator of gene expression; however, the molecular mechanisms linking pre-mRNA processing and Light Signaling are not well understood. Here we report a protein related to the human splicing factor 45 (SPF45) named splicing factor for phytochrome Signaling (SFPS), which directly interacts with the photoreceptor phytochrome B (phyB). In response to Light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP in photobodies. sfps loss-of-function plants are hyposensitive to red, far-red, and blue Light, and flower precociously. SFPS colocalizes with U2 small nuclear ribonucleoprotein-associated factors including U2AF65B, U2A′, and U2AF35A in nuclear speckles, suggesting SFPS might be involved in the 3′ splice site determination. SFPS regulates pre-mRNA splicing of a large number of genes, of which many are involved in regulating Light Signaling, photosynthesis, and the circadian clock under both dark and Light conditions. In vivo RNA immunoprecipitation (RIP) assays revealed that SFPS associates with EARLY FLOWERING 3 (ELF3) mRNA, a critical link between Light Signaling and the circadian clock. Moreover, PHYTOCHROME INTERACTING FACTORS (PIFs) transcription factor genes act downstream of SFPS, as the quadruple pif mutant pifq suppresses defects of sfps mutants. Taken together, these data strongly suggest SFPS modulates Light-regulated developmental processes by controlling pre-mRNA splicing of Light Signaling and circadian clock genes.
Frank G Harmon - One of the best experts on this subject based on the ideXlab platform.
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spf45 related splicing factor for phytochrome Signaling promotes photomorphogenesis by regulating pre mrna splicing in arabidopsis
Proceedings of the National Academy of Sciences of the United States of America, 2017Co-Authors: Patrice A Salome, Estefania Mancini, Carine M Marshall, Frank G Harmon, Marcelo J Yanovsky, Detlef WeigelAbstract:Abstract Light signals regulate plant growth and development by controlling a plethora of gene expression changes. Posttranscriptional regulation, especially pre-mRNA processing, is a key modulator of gene expression; however, the molecular mechanisms linking pre-mRNA processing and Light Signaling are not well understood. Here we report a protein related to the human splicing factor 45 (SPF45) named splicing factor for phytochrome Signaling (SFPS), which directly interacts with the photoreceptor phytochrome B (phyB). In response to Light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP in photobodies. sfps loss-of-function plants are hyposensitive to red, far-red, and blue Light, and flower precociously. SFPS colocalizes with U2 small nuclear ribonucleoprotein-associated factors including U2AF65B, U2A′, and U2AF35A in nuclear speckles, suggesting SFPS might be involved in the 3′ splice site determination. SFPS regulates pre-mRNA splicing of a large number of genes, of which many are involved in regulating Light Signaling, photosynthesis, and the circadian clock under both dark and Light conditions. In vivo RNA immunoprecipitation (RIP) assays revealed that SFPS associates with EARLY FLOWERING 3 (ELF3) mRNA, a critical link between Light Signaling and the circadian clock. Moreover, PHYTOCHROME INTERACTING FACTORS (PIFs) transcription factor genes act downstream of SFPS, as the quadruple pif mutant pifq suppresses defects of sfps mutants. Taken together, these data strongly suggest SFPS modulates Light-regulated developmental processes by controlling pre-mRNA splicing of Light Signaling and circadian clock genes.
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spf45 related splicing factor for phytochrome Signaling promotes photomorphogenesis by regulating pre mrna splicing in arabidopsis
Proceedings of the National Academy of Sciences of the United States of America, 2017Co-Authors: Patrice A Salome, Estefania Mancini, Carine M Marshall, Frank G Harmon, Marcelo J Yanovsky, Detlef Weigel, Ruijiao Xin, Ling ZhuAbstract:Abstract Light signals regulate plant growth and development by controlling a plethora of gene expression changes. Posttranscriptional regulation, especially pre-mRNA processing, is a key modulator of gene expression; however, the molecular mechanisms linking pre-mRNA processing and Light Signaling are not well understood. Here we report a protein related to the human splicing factor 45 (SPF45) named splicing factor for phytochrome Signaling (SFPS), which directly interacts with the photoreceptor phytochrome B (phyB). In response to Light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP in photobodies. sfps loss-of-function plants are hyposensitive to red, far-red, and blue Light, and flower precociously. SFPS colocalizes with U2 small nuclear ribonucleoprotein-associated factors including U2AF65B, U2A′, and U2AF35A in nuclear speckles, suggesting SFPS might be involved in the 3′ splice site determination. SFPS regulates pre-mRNA splicing of a large number of genes, of which many are involved in regulating Light Signaling, photosynthesis, and the circadian clock under both dark and Light conditions. In vivo RNA immunoprecipitation (RIP) assays revealed that SFPS associates with EARLY FLOWERING 3 (ELF3) mRNA, a critical link between Light Signaling and the circadian clock. Moreover, PHYTOCHROME INTERACTING FACTORS (PIFs) transcription factor genes act downstream of SFPS, as the quadruple pif mutant pifq suppresses defects of sfps mutants. Taken together, these data strongly suggest SFPS modulates Light-regulated developmental processes by controlling pre-mRNA splicing of Light Signaling and circadian clock genes.
Carine M Marshall - One of the best experts on this subject based on the ideXlab platform.
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spf45 related splicing factor for phytochrome Signaling promotes photomorphogenesis by regulating pre mrna splicing in arabidopsis
Proceedings of the National Academy of Sciences of the United States of America, 2017Co-Authors: Patrice A Salome, Estefania Mancini, Carine M Marshall, Frank G Harmon, Marcelo J Yanovsky, Detlef WeigelAbstract:Abstract Light signals regulate plant growth and development by controlling a plethora of gene expression changes. Posttranscriptional regulation, especially pre-mRNA processing, is a key modulator of gene expression; however, the molecular mechanisms linking pre-mRNA processing and Light Signaling are not well understood. Here we report a protein related to the human splicing factor 45 (SPF45) named splicing factor for phytochrome Signaling (SFPS), which directly interacts with the photoreceptor phytochrome B (phyB). In response to Light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP in photobodies. sfps loss-of-function plants are hyposensitive to red, far-red, and blue Light, and flower precociously. SFPS colocalizes with U2 small nuclear ribonucleoprotein-associated factors including U2AF65B, U2A′, and U2AF35A in nuclear speckles, suggesting SFPS might be involved in the 3′ splice site determination. SFPS regulates pre-mRNA splicing of a large number of genes, of which many are involved in regulating Light Signaling, photosynthesis, and the circadian clock under both dark and Light conditions. In vivo RNA immunoprecipitation (RIP) assays revealed that SFPS associates with EARLY FLOWERING 3 (ELF3) mRNA, a critical link between Light Signaling and the circadian clock. Moreover, PHYTOCHROME INTERACTING FACTORS (PIFs) transcription factor genes act downstream of SFPS, as the quadruple pif mutant pifq suppresses defects of sfps mutants. Taken together, these data strongly suggest SFPS modulates Light-regulated developmental processes by controlling pre-mRNA splicing of Light Signaling and circadian clock genes.
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spf45 related splicing factor for phytochrome Signaling promotes photomorphogenesis by regulating pre mrna splicing in arabidopsis
Proceedings of the National Academy of Sciences of the United States of America, 2017Co-Authors: Patrice A Salome, Estefania Mancini, Carine M Marshall, Frank G Harmon, Marcelo J Yanovsky, Detlef Weigel, Ruijiao Xin, Ling ZhuAbstract:Abstract Light signals regulate plant growth and development by controlling a plethora of gene expression changes. Posttranscriptional regulation, especially pre-mRNA processing, is a key modulator of gene expression; however, the molecular mechanisms linking pre-mRNA processing and Light Signaling are not well understood. Here we report a protein related to the human splicing factor 45 (SPF45) named splicing factor for phytochrome Signaling (SFPS), which directly interacts with the photoreceptor phytochrome B (phyB). In response to Light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP in photobodies. sfps loss-of-function plants are hyposensitive to red, far-red, and blue Light, and flower precociously. SFPS colocalizes with U2 small nuclear ribonucleoprotein-associated factors including U2AF65B, U2A′, and U2AF35A in nuclear speckles, suggesting SFPS might be involved in the 3′ splice site determination. SFPS regulates pre-mRNA splicing of a large number of genes, of which many are involved in regulating Light Signaling, photosynthesis, and the circadian clock under both dark and Light conditions. In vivo RNA immunoprecipitation (RIP) assays revealed that SFPS associates with EARLY FLOWERING 3 (ELF3) mRNA, a critical link between Light Signaling and the circadian clock. Moreover, PHYTOCHROME INTERACTING FACTORS (PIFs) transcription factor genes act downstream of SFPS, as the quadruple pif mutant pifq suppresses defects of sfps mutants. Taken together, these data strongly suggest SFPS modulates Light-regulated developmental processes by controlling pre-mRNA splicing of Light Signaling and circadian clock genes.
Marcelo J Yanovsky - One of the best experts on this subject based on the ideXlab platform.
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spf45 related splicing factor for phytochrome Signaling promotes photomorphogenesis by regulating pre mrna splicing in arabidopsis
Proceedings of the National Academy of Sciences of the United States of America, 2017Co-Authors: Patrice A Salome, Estefania Mancini, Carine M Marshall, Frank G Harmon, Marcelo J Yanovsky, Detlef WeigelAbstract:Abstract Light signals regulate plant growth and development by controlling a plethora of gene expression changes. Posttranscriptional regulation, especially pre-mRNA processing, is a key modulator of gene expression; however, the molecular mechanisms linking pre-mRNA processing and Light Signaling are not well understood. Here we report a protein related to the human splicing factor 45 (SPF45) named splicing factor for phytochrome Signaling (SFPS), which directly interacts with the photoreceptor phytochrome B (phyB). In response to Light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP in photobodies. sfps loss-of-function plants are hyposensitive to red, far-red, and blue Light, and flower precociously. SFPS colocalizes with U2 small nuclear ribonucleoprotein-associated factors including U2AF65B, U2A′, and U2AF35A in nuclear speckles, suggesting SFPS might be involved in the 3′ splice site determination. SFPS regulates pre-mRNA splicing of a large number of genes, of which many are involved in regulating Light Signaling, photosynthesis, and the circadian clock under both dark and Light conditions. In vivo RNA immunoprecipitation (RIP) assays revealed that SFPS associates with EARLY FLOWERING 3 (ELF3) mRNA, a critical link between Light Signaling and the circadian clock. Moreover, PHYTOCHROME INTERACTING FACTORS (PIFs) transcription factor genes act downstream of SFPS, as the quadruple pif mutant pifq suppresses defects of sfps mutants. Taken together, these data strongly suggest SFPS modulates Light-regulated developmental processes by controlling pre-mRNA splicing of Light Signaling and circadian clock genes.
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spf45 related splicing factor for phytochrome Signaling promotes photomorphogenesis by regulating pre mrna splicing in arabidopsis
Proceedings of the National Academy of Sciences of the United States of America, 2017Co-Authors: Patrice A Salome, Estefania Mancini, Carine M Marshall, Frank G Harmon, Marcelo J Yanovsky, Detlef Weigel, Ruijiao Xin, Ling ZhuAbstract:Abstract Light signals regulate plant growth and development by controlling a plethora of gene expression changes. Posttranscriptional regulation, especially pre-mRNA processing, is a key modulator of gene expression; however, the molecular mechanisms linking pre-mRNA processing and Light Signaling are not well understood. Here we report a protein related to the human splicing factor 45 (SPF45) named splicing factor for phytochrome Signaling (SFPS), which directly interacts with the photoreceptor phytochrome B (phyB). In response to Light, SFPS-RFP (red fluorescent protein) colocalizes with phyB-GFP in photobodies. sfps loss-of-function plants are hyposensitive to red, far-red, and blue Light, and flower precociously. SFPS colocalizes with U2 small nuclear ribonucleoprotein-associated factors including U2AF65B, U2A′, and U2AF35A in nuclear speckles, suggesting SFPS might be involved in the 3′ splice site determination. SFPS regulates pre-mRNA splicing of a large number of genes, of which many are involved in regulating Light Signaling, photosynthesis, and the circadian clock under both dark and Light conditions. In vivo RNA immunoprecipitation (RIP) assays revealed that SFPS associates with EARLY FLOWERING 3 (ELF3) mRNA, a critical link between Light Signaling and the circadian clock. Moreover, PHYTOCHROME INTERACTING FACTORS (PIFs) transcription factor genes act downstream of SFPS, as the quadruple pif mutant pifq suppresses defects of sfps mutants. Taken together, these data strongly suggest SFPS modulates Light-regulated developmental processes by controlling pre-mRNA splicing of Light Signaling and circadian clock genes.
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heat shock induced fluctuations in clock and Light Signaling enhance phytochrome b mediated arabidopsis deetiolation
The Plant Cell, 2013Co-Authors: Elizabeth Karayekov, Marcelo J Yanovsky, Martina Legris, Romina SellaroAbstract:Moderately warm constant ambient temperatures tend to oppose Light signals in the control of plant architecture. By contrast, here we show that brief heat shocks enhance the inhibition of hypocotyl growth induced by Light perceived by phytochrome B in deetiolating Arabidopsis thaliana seedlings. In darkness, daily heat shocks transiently increased the expression of PSEUDO-RESPONSE REGULATOR7 (PRR7) and PRR9 and markedly enhanced the amplitude of the rhythms of LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1) expression. In turn, these rhythms gated the hypocotyl response to red Light, in part by changing the expression of PHYTOCHROME INTERACTING FACTOR4 (PIF4) and PIF5. After Light exposure, heat shocks also reduced the nuclear abundance of CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) and increased the abundance of its target ELONGATED HYPOCOTYL5 (HY5). The synergism between Light and heat shocks was deficient in the prr7 prr9, lhy cca1, pif4 pif5, cop1, and hy5 mutants. The evening element (binding site of LHY and CCA1) and G-box promoter motifs (binding site of PIFs and HY5) were overrepresented among genes with expression controlled by both heat shock and red Light. The heat shocks experienced by buried seedlings approaching the surface of the soil prepare the seedlings for the impending exposure to Light by rhythmically lowering LHY, CCA1, PIF4, and PIF5 expression and by enhancing HY5 stability.