The Experts below are selected from a list of 2247 Experts worldwide ranked by ideXlab platform
N Miyata - One of the best experts on this subject based on the ideXlab platform.
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effect of 60 fullerene on the chondrogenesis in mouse embryonic Limb Bud Cell culture system
Fullerene Science and Technology, 1996Co-Authors: Toshie Tsuchiya, I Oguri, Nakajima Y Yamakoshi, N MiyataAbstract:Abstract [60] Fullerene solubilized with polyvinylpyrrolidone (PVP) promoted the mouse embryonic Limb Bud Cell differentiation specifically. The Cell lysis caused by C60 was diminished by the addition of superoxide dismutase and catalase.
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a novel promoting action of fullerene c60 on the chondrogenesis in rat embryonic Limb Bud Cell culture system
Biochemical and Biophysical Research Communications, 1995Co-Authors: Toshie Tsuchiya, Yoko Yamakoshi, N MiyataAbstract:Fullerene C60 was solubilized with polyvinylpyrrolidone (PVP) in water, and the aqueous solution was applied to the rat Limb Bud Cell differentiation system. By the incubation with various concentrations of C60 with poly(vinylpyrrolidone), Cell differentiation was extremely promoted, up to the levels of a 3.2-fold increase of the controls. Although poly(vinylpyrrolidone) alone inhibited the Cell differentiation in proportion to the concentration, the observed promoting action by C60 surpassed the action of poly(vinylpyrrolidone). This specific promoting action on the chondrogenesis is the novel significant activity of C60.
J Chevalier - One of the best experts on this subject based on the ideXlab platform.
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ultrastructural characterization of normal and abnormal chondrogenesis in micromass rat embryo Limb Bud Cell cultures
Toxicology and Applied Pharmacology, 1995Co-Authors: J Y Renault, Jean Michel Caillaud, J ChevalierAbstract:Abstract Inhibition of chondrogenesis in Limb Bud Cell micromass cultures has been proposed as a short-term teratogen detection test. Validation studies were performed by testing large series of reference compounds and comparing their teratogenic potential with their ability to inhibit chondrogenesis; however, there are few reports describing the histological and ultrastructural changes associated with inhibition of chondrogenesis in vitro . The objective of this study was to provide a qualitative description of the histological and ultrastructural alterations induced by three chondrogenesis inhibitors: retinoic acid (RA) and 6-aminonicotinamide (6AN), two teratogens, and doxylamine succinate (DS), a nonteratogen compound. In addition, in order to have a basis for the interpretation of the morphological alterations induced by the test compounds, the histological and ultrastructural changes which occur during the time course of chondrogenesis in control cultures were described and compared with those in rat embryo Limb Buds. We found that RA at 0.5 μg/ml led to a marked decrease in the number and size of cartilaginous foci; most Cells lacked morphological signs of differentiation but their ability to proliferate was unaffected. At concentrations of 2 μg/ml and more, 6AN delayed Cell proliferation, reduced staining of the extraCellular matrix, and induced the formation of endoplasmic cisternae. DS at 50 μg/ml affected both differentiation and proliferation; pigment deposits were observed in chondrocytes, suggesting phospholipid metabolism disorders. In conclusion, this study showed that inhibition of chondrogenesis in this simple Cell culture system can be associated with different types of histological and ultrastructural alterations. Examination of these alterations can provide useful information about the teratogenic potential of tested compounds and their mechanism of action.
Soma Yuko - One of the best experts on this subject based on the ideXlab platform.
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in vitro assessment of teratogenic potential of organotin compounds using rat embryo Limb Bud Cell cultures
Toxicology Letters, 1993Co-Authors: Yonemoto Junzo, Shiraishi Hiroaki, Soma YukoAbstract:Assessment of the relative teratogenic potential of bis(tri-n-butyltin)oxide (TBTO), tri-n-butyltin chloride (TBT), and its metabolites i.e., (3-OH)hydroxybutyl dibutyltin chloride ((3-OH-Bu)DBT), di-n-butyltin dichloride (DBT), and butyltin trichloride (MBT) have been conducted using rat embryo Limb Bud Cell cultures (LBC) to gain some knowledge of TBT embryotoxicity and DBT teratogenicity. Triphenyltin chloride (TPT), trimethyltin chloride (TMT), and triethyltin bromide (TET) have also been tested to obtain data for validation of LBC as a teratogen prescreening for organotin compounds. Fifty percent inhibition concentration for Cell proliferation (IP50), and for Cell differentiation (ID50), and the ratio of the former to the latter (P/D ratio) were obtained. The ID50 values in increasing order were as follows; TPT, DBT < TBT, TBTO < (3-OH-Bu)DBT < TET < TMT ⪡ MBT. With the exception of MBT, organotin compounds tested were very strong inhibitors of Cell differentiation (ID50; 0.13–1.71 μM) and Cell proliferation (IP50; 0.12–2.81μM). P/D ratios for TBT, (3-OH-Bu)DBT, DBT and MBT were 1.0, 1.43, 1.32 and 1.08, respectively. These results suggest that the proximal toxin of DBT teratogenicity is DBT itself, and TBT is rather embryocidal than teratogenic so that TBT might mask the teratogenic and/or fetotoxic effects of its metabolites.
Liu Hong - One of the best experts on this subject based on the ideXlab platform.
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effect of homocysteine on rat Limb Bud Cell proliferation and differentiation
Chinese Journal of Reproductive Health, 2000Co-Authors: Liu HongAbstract:Objective To determine potential mechanisms by which HCY may exerts its teratogenic effects. Method The micromass rat embryo Limb Bud Cell culture was used to reveal the differentiation and proliferation of the Cell under different HCY doses. Results With increased concentration, promotion effect changed into inhibition. The concentration inhibiting the formation of differential foci by 50% of the control is 5. 0 mmol/L and 3. 5 mmol/L when S9 was added; the concentration inhibiting the formation of proliferation by 50% of the control is 9.5mmol/L, when S9 is added , it was 5.8 mmol/L. Conclusion HCY obviously inhibited the Cell differentiation than proliferation, while it is not a potent teratogen for rat.
Arnold I. Caplan - One of the best experts on this subject based on the ideXlab platform.
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review mesenchymal stem Cells Cell based reconstructive therapy in orthopedics
Tissue Engineering, 2005Co-Authors: Arnold I. CaplanAbstract:Adult stem Cells provide replacement and repair descendants for normal turnover or injured tissues. These Cells have been isolated and expanded in culture, and their use for therapeutic strategies requires technologies not yet perfected. In the 1970s, the embryonic chick Limb Bud mesenchymal Cell culture system provided data on the differentiation of cartilage, bone, and muscle. In the 1980s, we used this Limb Bud Cell system as an assay for the purification of inductive factors in bone. In the 1990s, we used the expertise gained with embryonic mesenchymal progenitor Cells in culture to develop the technology for isolating, expanding, and preserving the stem Cell capacity of adult bone marrow-derived mesenchymal stem Cells (MSCs). The 1990s brought us into the new field of tissue engineering, where we used MSCs with site-specific delivery vehicles to repair cartilage, bone, tendon, marrow stroma, muscle, and other connective tissues. In the beginning of the 21st century, we have made substantial advances:...
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review mesenchymal stem Cells Cell based reconstructive therapy in orthopedics
Tissue Engineering, 2005Co-Authors: Arnold I. CaplanAbstract:Adult stem Cells provide replacement and repair descendants for normal turnover or injured tissues. These Cells have been isolated and expanded in culture, and their use for therapeutic strategies requires technologies not yet perfected. In the 1970s, the embryonic chick Limb Bud mesenchymal Cell culture system provided data on the differentiation of cartilage, bone, and muscle. In the 1980s, we used this Limb Bud Cell system as an assay for the purification of inductive factors in bone. In the 1990s, we used the expertise gained with embryonic mesenchymal progenitor Cells in culture to develop the technology for isolating, expanding, and preserving the stem Cell capacity of adult bone marrow-derived mesenchymal stem Cells (MSCs). The 1990s brought us into the new field of tissue engineering, where we used MSCs with site-specific delivery vehicles to repair cartilage, bone, tendon, marrow stroma, muscle, and other connective tissues. In the beginning of the 21st century, we have made substantial advances: the most important is the development of a Cell-coating technology, called painting, that allows us to introduce informational proteins to the outer surface of Cells. These paints can serve as targeting addresses to specifically dock MSCs or other reparative Cells to unique tissue addresses. The scientific and clinical challenge remains: to perfect Cell-based tissue-engineering protocols to utilize the body's own rejuvenation capabilities by managing surgical implantations of scaffolds, bioactive factors, and reparative Cells to regenerate damaged or diseased skeletal tissues.