The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform

Vincent Rioux - One of the best experts on this subject based on the ideXlab platform.

  • Effect of preduodenal Lipase inhibition in suckling rats on dietary octanoic acid (C8:0) gastric absorption and plasma octanoylated ghrelin concentration
    Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 2016
    Co-Authors: Fanny Lemarie, J. F. Cavalier, Cyrielle Garcia, F. Boissel, V. Point, Daniel Catheline, Philippe Legrand, F. Carriere, Vincent Rioux
    Abstract:

    Part of medium chain fatty acids (MCFAs) coming from dietary triglycerides (TGs) can be directly absorbed through the gastric mucosa after the action of preduodenal Lipase (Lingual Lipase in the rat). MCFA gastric absorption, particularly that of octanoic acid (C8:0), may have a physiological importance in the octanoylation of ghrelin, the orexigenic gastric peptide acting as an endogenous ligand of the hypothalamic growth hormone secretagogue receptor la (GHSR-1a). However, the amount of C8:0 absorbed in the stomach and its metabolic fate still haven't been clearly characterized. The purpose of the present study was to further characterize and quantify the importance of preduodenal Lipase activity on the release and gastric absorption of dietary C8:0 and on the subsequent ghrelin octanoylation in the stomach mucosa. Fifteen days old rats received fat emulsions containing triolein or [1,1,1-13C]-Tri-C8:0 and a specific inhibitor of preduodenal Lipase, 5-(2-(benzyloxy)ethoxy)-3-(3-phenoxyphenyl)-1,3,4-oxadiazol-2(3H)-one or BemPPDX. The fate of the C-13-C8:0 was followed in rat tissues after 30 and 120 min of digestion and octanoylated ghrelin was measured in the plasma. This work (1) demonstrates that part of C8:0 coming from Tri-C8:0 is directly absorbed at the gastric level, (2) allows the estimation of C8:0 gastric absorption level (1.3% of the C-13-C8:0 in sn-3 position after 30 min of digestion), as well as (3) the contribution of rat Lingual Lipase to total lipolysis and to duodenal absorption of dietary FAs (at least 30%), (4) shows no short-term effect of dietary Tri-C8:0 consumption and subsequent increase of C8:0 gastric tissue content on plasma octanoylated ghrelin concentration. (C) 2016 Elsevier B.V. All rights reserved.

Nada A Abumrad - One of the best experts on this subject based on the ideXlab platform.

  • The fatty acid translocase gene CD36 and Lingual Lipase influence oral sensitivity to fat in obese subjects
    Journal of Lipid Research, 2011
    Co-Authors: Marta Yanina Pepino, Latisha Love-gregory, Samuel Klein, Nada A Abumrad
    Abstract:

    The precise orosensory inputs engaged for dietary lipids detection in humans are unknown. We evaluated whether a common single nucleotide polymorphism (rs1761667) in the CD36 gene that reduces CD36 expression and the addition of orlistat, a Lipase inhibitor, to reduce FA release from triacylglycerols (TGs), the main component of dietary fats, would attenuate fat orosensory sensitivity in humans. Twenty-one obese subjects with different rs1761667 genotypes (6 AA, 7 AG, and 8 GG) were studied on two occasions in which oleic acid and triolein orosensory detection thresholds were measured using emulsions prepared with and without orlistat. Subjects homozygous for the G-allele had 8-fold lower oral detection thresholds for oleic acid and triolein than subjects homozygous for the A allele, which associates with lower CD36 expression (P = 0.03). Thresholds for heterozygous subjects were intermediate. The addition of orlistat increased detection thresholds for triolein (log threshold = −0.3 ± 0.2 vs. 0.3 ± 0.1; P 0.2). In conclusion, this is the first experimental evidence for a role of CD36 in fat gustatory perception in humans. The data also support involvement of Lingual Lipase and are consistent with the concept that FA and not TG is the sensed stimulus.

Fanny Lemarie - One of the best experts on this subject based on the ideXlab platform.

  • Effect of preduodenal Lipase inhibition in suckling rats on dietary octanoic acid (C8:0) gastric absorption and plasma octanoylated ghrelin concentration
    Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 2016
    Co-Authors: Fanny Lemarie, J. F. Cavalier, Cyrielle Garcia, F. Boissel, V. Point, Daniel Catheline, Philippe Legrand, F. Carriere, Vincent Rioux
    Abstract:

    Part of medium chain fatty acids (MCFAs) coming from dietary triglycerides (TGs) can be directly absorbed through the gastric mucosa after the action of preduodenal Lipase (Lingual Lipase in the rat). MCFA gastric absorption, particularly that of octanoic acid (C8:0), may have a physiological importance in the octanoylation of ghrelin, the orexigenic gastric peptide acting as an endogenous ligand of the hypothalamic growth hormone secretagogue receptor la (GHSR-1a). However, the amount of C8:0 absorbed in the stomach and its metabolic fate still haven't been clearly characterized. The purpose of the present study was to further characterize and quantify the importance of preduodenal Lipase activity on the release and gastric absorption of dietary C8:0 and on the subsequent ghrelin octanoylation in the stomach mucosa. Fifteen days old rats received fat emulsions containing triolein or [1,1,1-13C]-Tri-C8:0 and a specific inhibitor of preduodenal Lipase, 5-(2-(benzyloxy)ethoxy)-3-(3-phenoxyphenyl)-1,3,4-oxadiazol-2(3H)-one or BemPPDX. The fate of the C-13-C8:0 was followed in rat tissues after 30 and 120 min of digestion and octanoylated ghrelin was measured in the plasma. This work (1) demonstrates that part of C8:0 coming from Tri-C8:0 is directly absorbed at the gastric level, (2) allows the estimation of C8:0 gastric absorption level (1.3% of the C-13-C8:0 in sn-3 position after 30 min of digestion), as well as (3) the contribution of rat Lingual Lipase to total lipolysis and to duodenal absorption of dietary FAs (at least 30%), (4) shows no short-term effect of dietary Tri-C8:0 consumption and subsequent increase of C8:0 gastric tissue content on plasma octanoylated ghrelin concentration. (C) 2016 Elsevier B.V. All rights reserved.

Marta Yanina Pepino - One of the best experts on this subject based on the ideXlab platform.

  • The fatty acid translocase gene CD36 and Lingual Lipase influence oral sensitivity to fat in obese subjects
    Journal of Lipid Research, 2011
    Co-Authors: Marta Yanina Pepino, Latisha Love-gregory, Samuel Klein, Nada A Abumrad
    Abstract:

    The precise orosensory inputs engaged for dietary lipids detection in humans are unknown. We evaluated whether a common single nucleotide polymorphism (rs1761667) in the CD36 gene that reduces CD36 expression and the addition of orlistat, a Lipase inhibitor, to reduce FA release from triacylglycerols (TGs), the main component of dietary fats, would attenuate fat orosensory sensitivity in humans. Twenty-one obese subjects with different rs1761667 genotypes (6 AA, 7 AG, and 8 GG) were studied on two occasions in which oleic acid and triolein orosensory detection thresholds were measured using emulsions prepared with and without orlistat. Subjects homozygous for the G-allele had 8-fold lower oral detection thresholds for oleic acid and triolein than subjects homozygous for the A allele, which associates with lower CD36 expression (P = 0.03). Thresholds for heterozygous subjects were intermediate. The addition of orlistat increased detection thresholds for triolein (log threshold = −0.3 ± 0.2 vs. 0.3 ± 0.1; P 0.2). In conclusion, this is the first experimental evidence for a role of CD36 in fat gustatory perception in humans. The data also support involvement of Lingual Lipase and are consistent with the concept that FA and not TG is the sensed stimulus.

F. Carriere - One of the best experts on this subject based on the ideXlab platform.

  • Effect of preduodenal Lipase inhibition in suckling rats on dietary octanoic acid (C8:0) gastric absorption and plasma octanoylated ghrelin concentration
    Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 2016
    Co-Authors: Fanny Lemarie, J. F. Cavalier, Cyrielle Garcia, F. Boissel, V. Point, Daniel Catheline, Philippe Legrand, F. Carriere, Vincent Rioux
    Abstract:

    Part of medium chain fatty acids (MCFAs) coming from dietary triglycerides (TGs) can be directly absorbed through the gastric mucosa after the action of preduodenal Lipase (Lingual Lipase in the rat). MCFA gastric absorption, particularly that of octanoic acid (C8:0), may have a physiological importance in the octanoylation of ghrelin, the orexigenic gastric peptide acting as an endogenous ligand of the hypothalamic growth hormone secretagogue receptor la (GHSR-1a). However, the amount of C8:0 absorbed in the stomach and its metabolic fate still haven't been clearly characterized. The purpose of the present study was to further characterize and quantify the importance of preduodenal Lipase activity on the release and gastric absorption of dietary C8:0 and on the subsequent ghrelin octanoylation in the stomach mucosa. Fifteen days old rats received fat emulsions containing triolein or [1,1,1-13C]-Tri-C8:0 and a specific inhibitor of preduodenal Lipase, 5-(2-(benzyloxy)ethoxy)-3-(3-phenoxyphenyl)-1,3,4-oxadiazol-2(3H)-one or BemPPDX. The fate of the C-13-C8:0 was followed in rat tissues after 30 and 120 min of digestion and octanoylated ghrelin was measured in the plasma. This work (1) demonstrates that part of C8:0 coming from Tri-C8:0 is directly absorbed at the gastric level, (2) allows the estimation of C8:0 gastric absorption level (1.3% of the C-13-C8:0 in sn-3 position after 30 min of digestion), as well as (3) the contribution of rat Lingual Lipase to total lipolysis and to duodenal absorption of dietary FAs (at least 30%), (4) shows no short-term effect of dietary Tri-C8:0 consumption and subsequent increase of C8:0 gastric tissue content on plasma octanoylated ghrelin concentration. (C) 2016 Elsevier B.V. All rights reserved.