The Experts below are selected from a list of 87 Experts worldwide ranked by ideXlab platform

Yasushi Saeki - One of the best experts on this subject based on the ideXlab platform.

  • in vivo ubiquitin linkage type analysis reveals that the cdc48 rad23 dsk2 axis contributes to k48 Linked Chain specificity of the proteasome
    Molecular Cell, 2017
    Co-Authors: Hikaru Tsuchiya, Fumiaki Ohtake, Naoko Arai, Ai Kaiho, Sayaka Yasuda, Keiji Tanaka, Yasushi Saeki
    Abstract:

    Summary Ubiquitin-binding domain (UBD) proteins regulate numerous cellular processes, but their specificities toward ubiquitin Chain types in cells remain obscure. Here, we perform a quantitative proteomic analysis of ubiquitin linkage-type selectivity of 14 UBD proteins and the proteasome in yeast. We find that K48-Linked Chains are directed to proteasomal degradation through selectivity of the Cdc48 cofactor Npl4. Mutating Cdc48 results in decreased selectivity, and lacking Rad23/Dsk2 abolishes interactions between ubiquitylated substrates and the proteasome. Among them, only Npl4 has K48 Chain specificity in vitro. Thus, the Cdc48 complex functions as a K48 linkage-specifying factor upstream of Rad23/Dsk2 for proteasomal degradation. On the other hand, K63 Chains are utilized in endocytic pathways, whereas both K48 and K63 Chains are found in the MVB and autophagic pathways. Collectively, our results provide an overall picture of the ubiquitin network via UBD proteins and identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.

  • In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome
    Molecular Cell, 2017
    Co-Authors: Hikaru Tsuchiya, Fumiaki Ohtake, Naoko Arai, Ai Kaiho, Sayaka Yasuda, Keiji Tanaka, Yasushi Saeki
    Abstract:

    Summary Ubiquitin-binding domain (UBD) proteins regulate numerous cellular processes, but their specificities toward ubiquitin Chain types in cells remain obscure. Here, we perform a quantitative proteomic analysis of ubiquitin linkage-type selectivity of 14 UBD proteins and the proteasome in yeast. We find that K48-Linked Chains are directed to proteasomal degradation through selectivity of the Cdc48 cofactor Npl4. Mutating Cdc48 results in decreased selectivity, and lacking Rad23/Dsk2 abolishes interactions between ubiquitylated substrates and the proteasome. Among them, only Npl4 has K48 Chain specificity in vitro. Thus, the Cdc48 complex functions as a K48 linkage-specifying factor upstream of Rad23/Dsk2 for proteasomal degradation. On the other hand, K63 Chains are utilized in endocytic pathways, whereas both K48 and K63 Chains are found in the MVB and autophagic pathways. Collectively, our results provide an overall picture of the ubiquitin network via UBD proteins and identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.

Wan Daud Wan Ramli - One of the best experts on this subject based on the ideXlab platform.

  • PTFE-nafion membrane reactor for hydrogen production
    International Journal of Hydrogen Energy, 2013
    Co-Authors: T. Husaini, Majlan Edy Herianto, Yaakob Zahira, Wan Daud Wan Ramli
    Abstract:

    This paper presents an experimental and modelling study of the kinetics of hydrogen production through the methanol steam reforming (MSR) process using a membrane reactor with a self-made, porous PTFE-nafion membrane. The operating conditions were optimised through an analysis of the hydrogen recovery and the CO selectivity. The membrane reactor was modelled based on the hydrogen production rate, which was Linked (Chain) to the changes in the reactant concentrations. The analysis of the reaction kinetics provided an overview of the reaction process and the factors that affect the process. Moreover, the size of the membrane was estimated using the distribution of hydrogen concentrations along the reactor. The separation factors, particularly the separation of hydrogen from CO and CO2, are some of the factors that can influence the performance of a membrane reactor. Copyright © 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Hikaru Tsuchiya - One of the best experts on this subject based on the ideXlab platform.

  • in vivo ubiquitin linkage type analysis reveals that the cdc48 rad23 dsk2 axis contributes to k48 Linked Chain specificity of the proteasome
    Molecular Cell, 2017
    Co-Authors: Hikaru Tsuchiya, Fumiaki Ohtake, Naoko Arai, Ai Kaiho, Sayaka Yasuda, Keiji Tanaka, Yasushi Saeki
    Abstract:

    Summary Ubiquitin-binding domain (UBD) proteins regulate numerous cellular processes, but their specificities toward ubiquitin Chain types in cells remain obscure. Here, we perform a quantitative proteomic analysis of ubiquitin linkage-type selectivity of 14 UBD proteins and the proteasome in yeast. We find that K48-Linked Chains are directed to proteasomal degradation through selectivity of the Cdc48 cofactor Npl4. Mutating Cdc48 results in decreased selectivity, and lacking Rad23/Dsk2 abolishes interactions between ubiquitylated substrates and the proteasome. Among them, only Npl4 has K48 Chain specificity in vitro. Thus, the Cdc48 complex functions as a K48 linkage-specifying factor upstream of Rad23/Dsk2 for proteasomal degradation. On the other hand, K63 Chains are utilized in endocytic pathways, whereas both K48 and K63 Chains are found in the MVB and autophagic pathways. Collectively, our results provide an overall picture of the ubiquitin network via UBD proteins and identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.

  • In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome
    Molecular Cell, 2017
    Co-Authors: Hikaru Tsuchiya, Fumiaki Ohtake, Naoko Arai, Ai Kaiho, Sayaka Yasuda, Keiji Tanaka, Yasushi Saeki
    Abstract:

    Summary Ubiquitin-binding domain (UBD) proteins regulate numerous cellular processes, but their specificities toward ubiquitin Chain types in cells remain obscure. Here, we perform a quantitative proteomic analysis of ubiquitin linkage-type selectivity of 14 UBD proteins and the proteasome in yeast. We find that K48-Linked Chains are directed to proteasomal degradation through selectivity of the Cdc48 cofactor Npl4. Mutating Cdc48 results in decreased selectivity, and lacking Rad23/Dsk2 abolishes interactions between ubiquitylated substrates and the proteasome. Among them, only Npl4 has K48 Chain specificity in vitro. Thus, the Cdc48 complex functions as a K48 linkage-specifying factor upstream of Rad23/Dsk2 for proteasomal degradation. On the other hand, K63 Chains are utilized in endocytic pathways, whereas both K48 and K63 Chains are found in the MVB and autophagic pathways. Collectively, our results provide an overall picture of the ubiquitin network via UBD proteins and identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.

T. Husaini - One of the best experts on this subject based on the ideXlab platform.

  • PTFE-nafion membrane reactor for hydrogen production
    International Journal of Hydrogen Energy, 2013
    Co-Authors: T. Husaini, Yaakob Zahira, Majlan Edy Herianto, Wan Daud Wan Ramli
    Abstract:

    Abstract This paper presents an experimental and modelling study of the kinetics of hydrogen production through the methanol steam reforming (MSR) process using a membrane reactor with a self-made, porous PTFE-nafion membrane. The operating conditions were optimised through an analysis of the hydrogen recovery and the CO selectivity. The membrane reactor was modelled based on the hydrogen production rate, which was Linked (Chain) to the changes in the reactant concentrations. The analysis of the reaction kinetics provided an overview of the reaction process and the factors that affect the process. Moreover, the size of the membrane was estimated using the distribution of hydrogen concentrations along the reactor. The separation factors, particularly the separation of hydrogen from CO and CO2, are some of the factors that can influence the performance of a membrane reactor.

  • PTFE-nafion membrane reactor for hydrogen production
    International Journal of Hydrogen Energy, 2013
    Co-Authors: T. Husaini, Majlan Edy Herianto, Yaakob Zahira, Wan Daud Wan Ramli
    Abstract:

    This paper presents an experimental and modelling study of the kinetics of hydrogen production through the methanol steam reforming (MSR) process using a membrane reactor with a self-made, porous PTFE-nafion membrane. The operating conditions were optimised through an analysis of the hydrogen recovery and the CO selectivity. The membrane reactor was modelled based on the hydrogen production rate, which was Linked (Chain) to the changes in the reactant concentrations. The analysis of the reaction kinetics provided an overview of the reaction process and the factors that affect the process. Moreover, the size of the membrane was estimated using the distribution of hydrogen concentrations along the reactor. The separation factors, particularly the separation of hydrogen from CO and CO2, are some of the factors that can influence the performance of a membrane reactor. Copyright © 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Fumiaki Ohtake - One of the best experts on this subject based on the ideXlab platform.

  • in vivo ubiquitin linkage type analysis reveals that the cdc48 rad23 dsk2 axis contributes to k48 Linked Chain specificity of the proteasome
    Molecular Cell, 2017
    Co-Authors: Hikaru Tsuchiya, Fumiaki Ohtake, Naoko Arai, Ai Kaiho, Sayaka Yasuda, Keiji Tanaka, Yasushi Saeki
    Abstract:

    Summary Ubiquitin-binding domain (UBD) proteins regulate numerous cellular processes, but their specificities toward ubiquitin Chain types in cells remain obscure. Here, we perform a quantitative proteomic analysis of ubiquitin linkage-type selectivity of 14 UBD proteins and the proteasome in yeast. We find that K48-Linked Chains are directed to proteasomal degradation through selectivity of the Cdc48 cofactor Npl4. Mutating Cdc48 results in decreased selectivity, and lacking Rad23/Dsk2 abolishes interactions between ubiquitylated substrates and the proteasome. Among them, only Npl4 has K48 Chain specificity in vitro. Thus, the Cdc48 complex functions as a K48 linkage-specifying factor upstream of Rad23/Dsk2 for proteasomal degradation. On the other hand, K63 Chains are utilized in endocytic pathways, whereas both K48 and K63 Chains are found in the MVB and autophagic pathways. Collectively, our results provide an overall picture of the ubiquitin network via UBD proteins and identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.

  • In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome
    Molecular Cell, 2017
    Co-Authors: Hikaru Tsuchiya, Fumiaki Ohtake, Naoko Arai, Ai Kaiho, Sayaka Yasuda, Keiji Tanaka, Yasushi Saeki
    Abstract:

    Summary Ubiquitin-binding domain (UBD) proteins regulate numerous cellular processes, but their specificities toward ubiquitin Chain types in cells remain obscure. Here, we perform a quantitative proteomic analysis of ubiquitin linkage-type selectivity of 14 UBD proteins and the proteasome in yeast. We find that K48-Linked Chains are directed to proteasomal degradation through selectivity of the Cdc48 cofactor Npl4. Mutating Cdc48 results in decreased selectivity, and lacking Rad23/Dsk2 abolishes interactions between ubiquitylated substrates and the proteasome. Among them, only Npl4 has K48 Chain specificity in vitro. Thus, the Cdc48 complex functions as a K48 linkage-specifying factor upstream of Rad23/Dsk2 for proteasomal degradation. On the other hand, K63 Chains are utilized in endocytic pathways, whereas both K48 and K63 Chains are found in the MVB and autophagic pathways. Collectively, our results provide an overall picture of the ubiquitin network via UBD proteins and identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.