The Experts below are selected from a list of 45096 Experts worldwide ranked by ideXlab platform
Annika Enejder - One of the best experts on this subject based on the ideXlab platform.
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monitoring of Lipid Storage in caenorhabditis elegans using coherent anti stokes raman scattering cars microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: Thomas Hellerer, Claes Axang, Christian Brackmann, Per Hillertz, Marc Pilon, Annika EnejderAbstract:Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor Lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of Lipids compared with coherent anti-Stokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on Lipid Storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a Lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-β) signaling pathways had Lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized Lipid droplets in the hypodermal cells, hosting as much as 40% of the total Lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the Lipids from gel to liquid phase. We conclude that the degree of hypodermal Lipid Storage and the Lipid phase can be used as a marker of Lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of Lipid Storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.
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monitoring of Lipid Storage in caenorhabditis elegans using coherent anti stokes raman scattering cars microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: Thomas Hellerer, Claes Axang, Christian Brackmann, Per Hillertz, Marc Pilon, Annika EnejderAbstract:Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor Lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of Lipids compared with coherent anti-Stokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on Lipid Storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a Lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-beta) signaling pathways had Lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized Lipid droplets in the hypodermal cells, hosting as much as 40% of the total Lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the Lipids from gel to liquid phase. We conclude that the degree of hypodermal Lipid Storage and the Lipid phase can be used as a marker of Lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of Lipid Storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.
Thomas Hellerer - One of the best experts on this subject based on the ideXlab platform.
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monitoring of Lipid Storage in caenorhabditis elegans using coherent anti stokes raman scattering cars microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: Thomas Hellerer, Claes Axang, Christian Brackmann, Per Hillertz, Marc Pilon, Annika EnejderAbstract:Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor Lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of Lipids compared with coherent anti-Stokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on Lipid Storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a Lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-β) signaling pathways had Lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized Lipid droplets in the hypodermal cells, hosting as much as 40% of the total Lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the Lipids from gel to liquid phase. We conclude that the degree of hypodermal Lipid Storage and the Lipid phase can be used as a marker of Lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of Lipid Storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.
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monitoring of Lipid Storage in caenorhabditis elegans using coherent anti stokes raman scattering cars microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: Thomas Hellerer, Claes Axang, Christian Brackmann, Per Hillertz, Marc Pilon, Annika EnejderAbstract:Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor Lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of Lipids compared with coherent anti-Stokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on Lipid Storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a Lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-beta) signaling pathways had Lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized Lipid droplets in the hypodermal cells, hosting as much as 40% of the total Lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the Lipids from gel to liquid phase. We conclude that the degree of hypodermal Lipid Storage and the Lipid phase can be used as a marker of Lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of Lipid Storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.
Marcello Arca - One of the best experts on this subject based on the ideXlab platform.
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metabolic consequences of adipose triglyceride lipase deficiency in humans an in vivo study in patients with neutral Lipid Storage disease with myopathy
The Journal of Clinical Endocrinology and Metabolism, 2013Co-Authors: Andrea Natali, Fabiana Quagliarini, Elena Maria Pennisi, Claudio Bruno, Amalia Gastaldelli, Stefania Camastra, Simona Baldi, Ilenia Minicocci, Marcello ArcaAbstract:Context: The role of adipose triglyceride lipase (ATGL) in intermediate substrates metabolism has not been fully elucidated in humans. Objective: Our objective was to evaluate the consequences of ATGL deficiency on body fat distribution, insulin sensitivity, fatty acids metabolism, and energy substrate utilization. Design and Setting: Body composition and organ fat content were measured by bioimpedance and 1H nuclear magnetic resonance spectroscopy; heart glucose metabolism by [18F]deoxyglucose positron emission tomography and insulin sensitivity and β-cell function by oral glucose tolerance and 2-step euglycemic-hyperinsulinemic clamp. Lipolysis ([2H5]glycerol turnover) and indirect calorimetry were evaluated at fasting, after oral glucose load, during the clamp, and also during an iv epinephrine infusion. These metabolic investigations were carried out during hospitalization. Patients: Three patients affected by neutral Lipid Storage disease with myopathy (NLSDM) due to homozygosity for loss-of-function...
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Novel mutations in the adipose triglyceride lipase gene causing neutral Lipid Storage disease with myopathy.
Biochemical and biophysical research communications, 2008Co-Authors: Filomena Campagna, Salvatore Dimauro, Luisa Nanni, Fabiana Quagliarini, Elena Maria Pennisi, Constantine Michailidis, Francesco Pierelli, Claudio Bruno, Carlo Casali, Marcello ArcaAbstract:Abstract A subgroup of neutral Lipid Storage disease has been recently associated with myopathy (NLSDM) and attributed to mutations in the gene (PNPLA2) encoding an adipose triglyceride lipase involved in the degradation of intracellular triglycerides. Five NLSDM patients have been described thus far and we reported three additional patients. A 44-year old Iranian woman and two Italian brothers, aged 40 and 35, presented with exercise intolerance and proximal limb weakness, elevated CK levels, and Jordan’s anomaly. Muscle biopsies showed marked neutral Lipid accumulation in all patients. The 10 exons and the intron–exon junctions of the PNPLA2 gene were sequenced. Two novel homozygous mutations in exon 5 of PNPLA2 gene were found (c.695delT and c.542delAC). Both mutations resulted in frameshifts leading to premature stop codons (p.L255X and p.I212X, respectively). These mutations predict a truncated PNPLA2 protein lacking the C-terminal hydrophobic domain. These findings indicate that NLSDM is rare, but genetically heterogeneous.
Claes Axang - One of the best experts on this subject based on the ideXlab platform.
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monitoring of Lipid Storage in caenorhabditis elegans using coherent anti stokes raman scattering cars microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: Thomas Hellerer, Claes Axang, Christian Brackmann, Per Hillertz, Marc Pilon, Annika EnejderAbstract:Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor Lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of Lipids compared with coherent anti-Stokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on Lipid Storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a Lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-β) signaling pathways had Lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized Lipid droplets in the hypodermal cells, hosting as much as 40% of the total Lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the Lipids from gel to liquid phase. We conclude that the degree of hypodermal Lipid Storage and the Lipid phase can be used as a marker of Lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of Lipid Storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.
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monitoring of Lipid Storage in caenorhabditis elegans using coherent anti stokes raman scattering cars microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: Thomas Hellerer, Claes Axang, Christian Brackmann, Per Hillertz, Marc Pilon, Annika EnejderAbstract:Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor Lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of Lipids compared with coherent anti-Stokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on Lipid Storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a Lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-beta) signaling pathways had Lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized Lipid droplets in the hypodermal cells, hosting as much as 40% of the total Lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the Lipids from gel to liquid phase. We conclude that the degree of hypodermal Lipid Storage and the Lipid phase can be used as a marker of Lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of Lipid Storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.
Christian Brackmann - One of the best experts on this subject based on the ideXlab platform.
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monitoring of Lipid Storage in caenorhabditis elegans using coherent anti stokes raman scattering cars microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: Thomas Hellerer, Claes Axang, Christian Brackmann, Per Hillertz, Marc Pilon, Annika EnejderAbstract:Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor Lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of Lipids compared with coherent anti-Stokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on Lipid Storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a Lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-β) signaling pathways had Lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized Lipid droplets in the hypodermal cells, hosting as much as 40% of the total Lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the Lipids from gel to liquid phase. We conclude that the degree of hypodermal Lipid Storage and the Lipid phase can be used as a marker of Lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of Lipid Storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.
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monitoring of Lipid Storage in caenorhabditis elegans using coherent anti stokes raman scattering cars microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2007Co-Authors: Thomas Hellerer, Claes Axang, Christian Brackmann, Per Hillertz, Marc Pilon, Annika EnejderAbstract:Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor Lipid stores on single-cell level in vivo. We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescence microscopy for imaging of Lipids compared with coherent anti-Stokes Raman scattering (CARS) microscopy, the latter allowing chemically specific and label-free imaging in living organisms. CARS microscopy was used to quantitatively monitor the impact of genetic variations in metabolic pathways on Lipid Storage in 60 specimens of C. elegans. We found that the feeding-defective mutant pha-3 contained a Lipid volume fraction one-third of that found in control worms. In contrast, mutants (daf-2, daf-4 dauer) with deficiencies in the insulin and transforming growth factors (IGF and TGF-beta) signaling pathways had Lipid volume fractions that were 1.4 and 2 times larger than controls, respectively. This was observed as an accumulation of small-sized Lipid droplets in the hypodermal cells, hosting as much as 40% of the total Lipid volume in contrast to the 9% for the wild-type larvae. Spectral CARS microscopy measurements indicated that this is accompanied by a shift in the ordering of the Lipids from gel to liquid phase. We conclude that the degree of hypodermal Lipid Storage and the Lipid phase can be used as a marker of Lipid metabolism shift. This study shows that CARS microscopy has the potential to become a sensitive and important tool for studies of Lipid Storage mechanisms, improving our understanding of phenomena underlying metabolic disorders.