The Experts below are selected from a list of 5613 Experts worldwide ranked by ideXlab platform
Andrzej Przemyslaw Herman - One of the best experts on this subject based on the ideXlab platform.
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essential oils and their constituents as skin penetration enhancer for transdermal drug delivery a review
Journal of Pharmacy and Pharmacology, 2015Co-Authors: Anna Herman, Andrzej Przemyslaw HermanAbstract:Objectives In this paper, we focused on essential oils and their constituents as skin penetration enhancers for transdermal drug delivery, mechanism of their action as well as their possible toxicity. Key findings Essential oils and their volatile constituents can penetrate through the skin as well as enhance penetration of different drug from topical formulation into the lower skin layers using different mechanisms of action based on (1) disintegration of the highly ordered intercellular Lipid Structure between corneocytes in stratum corneum, (2) interaction with intercellular domain of protein, which induces their conformational modification, (3) increase the partitioning of a drug. After application to the skin, essential oils and their components are rapidly metabolized, not accumulated in the organism and fast excreted what strongly suggest that they can be successfully use as safe penetration enhancers. Summary Essential oils and their constituents may be preferred over the traditionally used synthetics materials as safe and suitable permeation enhancers to promote the percutaneous absorption of hydrophilic and lipophilic drugs from topical formulation into the lower skin layers.
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essential oils and their constituents as skin penetration enhancer for transdermal drug delivery a review
Journal of Pharmacy and Pharmacology, 2015Co-Authors: Anna Herman, Andrzej Przemyslaw HermanAbstract:Objectives In this paper, we focused on essential oils and their constituents as skin penetration enhancers for transdermal drug delivery, mechanism of their action as well as their possible toxicity. Key findings Essential oils and their volatile constituents can penetrate through the skin as well as enhance penetration of different drug from topical formulation into the lower skin layers using different mechanisms of action based on (1) disintegration of the highly ordered intercellular Lipid Structure between corneocytes in stratum corneum, (2) interaction with intercellular domain of protein, which induces their conformational modification, (3) increase the partitioning of a drug. After application to the skin, essential oils and their components are rapidly metabolized, not accumulated in the organism and fast excreted what strongly suggest that they can be successfully use as safe penetration enhancers. Summary Essential oils and their constituents may be preferred over the traditionally used synthetics materials as safe and suitable permeation enhancers to promote the percutaneous absorption of hydrophilic and lipophilic drugs from topical formulation into the lower skin layers.
Anna Herman - One of the best experts on this subject based on the ideXlab platform.
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essential oils and their constituents as skin penetration enhancer for transdermal drug delivery a review
Journal of Pharmacy and Pharmacology, 2015Co-Authors: Anna Herman, Andrzej Przemyslaw HermanAbstract:Objectives In this paper, we focused on essential oils and their constituents as skin penetration enhancers for transdermal drug delivery, mechanism of their action as well as their possible toxicity. Key findings Essential oils and their volatile constituents can penetrate through the skin as well as enhance penetration of different drug from topical formulation into the lower skin layers using different mechanisms of action based on (1) disintegration of the highly ordered intercellular Lipid Structure between corneocytes in stratum corneum, (2) interaction with intercellular domain of protein, which induces their conformational modification, (3) increase the partitioning of a drug. After application to the skin, essential oils and their components are rapidly metabolized, not accumulated in the organism and fast excreted what strongly suggest that they can be successfully use as safe penetration enhancers. Summary Essential oils and their constituents may be preferred over the traditionally used synthetics materials as safe and suitable permeation enhancers to promote the percutaneous absorption of hydrophilic and lipophilic drugs from topical formulation into the lower skin layers.
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essential oils and their constituents as skin penetration enhancer for transdermal drug delivery a review
Journal of Pharmacy and Pharmacology, 2015Co-Authors: Anna Herman, Andrzej Przemyslaw HermanAbstract:Objectives In this paper, we focused on essential oils and their constituents as skin penetration enhancers for transdermal drug delivery, mechanism of their action as well as their possible toxicity. Key findings Essential oils and their volatile constituents can penetrate through the skin as well as enhance penetration of different drug from topical formulation into the lower skin layers using different mechanisms of action based on (1) disintegration of the highly ordered intercellular Lipid Structure between corneocytes in stratum corneum, (2) interaction with intercellular domain of protein, which induces their conformational modification, (3) increase the partitioning of a drug. After application to the skin, essential oils and their components are rapidly metabolized, not accumulated in the organism and fast excreted what strongly suggest that they can be successfully use as safe penetration enhancers. Summary Essential oils and their constituents may be preferred over the traditionally used synthetics materials as safe and suitable permeation enhancers to promote the percutaneous absorption of hydrophilic and lipophilic drugs from topical formulation into the lower skin layers.
Olga López - One of the best experts on this subject based on the ideXlab platform.
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lamellar body mimetic system an up to down repairing strategy of the stratum corneum Lipid Structure
International Journal of Pharmaceutics, 2016Co-Authors: Veronica Moner, Gelen Rodríguez, Mercedes Cócera, Estibalitz Fernandez, Lucyanna Barbosabarros, Alfonso De La Maza, Olga LópezAbstract:Abstract Epidermal lamellar bodies (LBs) are organelles that secrete their content, mainly Lipids and enzymes, into the intercorneocyte space of the stratum corneum (SC) to form the lamellar Structure of this tissue. Thus, LBs have a key role in permeability and the microbial cutaneous barrier. In this work, a complex Lipid system that mimics the morphology, Structure and composition of LBs has been designed. To evaluate the effect of this system on deLipidized SC, in vitro experiments using porcine skin were performed. The microStructure of SC samples (native, deLipidized and, deLipidized after treatment) was evaluated by freeze substitution transmission electron microscopy (FSTEM) and grazing-incidence small-angle X-ray scattering (GISAXS). DeLipidized SC samples showed no evidence of Lipid lamellae after extraction with organic solvents. However, after treatment with the LB mimetic system, new lamellar Structures between corneocytes were detected by FSTEM, and high intensity peaks and reflections were found in the GISAXS pattern. These results demonstrate a strong effect of the treatment in repairing part of the Lipid lamellar Structure of the SC. Accordingly, future research could extend the use of this system to repair skin barrier dysfunction.
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Bicellar systems as modifiers of skin Lipid Structure
Colloids and Surfaces B: Biointerfaces, 2011Co-Authors: Gelen Rodríguez, Mercedes Cócera, Aude Maza, L. Rubio, L. Barbosa-barros, Carmen López-iglesias, Olga LópezAbstract:Abstract The characterization of different bicellar aggregates and the effects of these systems on the stratum corneum (SC) microStructure have been studied. Dynamic light scattering (DLS) and freeze fracture electron microscopy (FFEM) techniques showed that both of the systems studied, dimyristoyl-phosphatidilcholine/dihexanoyl-phosphocholine (DMPC/DHPC) and dipalmitoyl-phosphocholine (DPPC)/DHPC, were formed by small discoidal aggregates at room temperature (20 °C). Treating skin with DMPC/DHPC bicelles does not affect the SC Lipid microStructure, whereas bicellar systems formed by DPPC and DHPC can promote the formation of new Structures in the SC Lipid domains. This indicates the passage of Lipids from bicelles through the SC layers and also a possible interaction of these Lipids with the SC Lipids. Given the absence of surfactant in the bicellar composition and the small size of these Structures, the use of these smart nano-systems offers great advantages over other Lipid systems for dermatological purposes. Bicelles could be promising applications as drug carriers through the skin. This contribution, based on the new biological use of bicelles, may be useful to scientists engaged in colloid science and offers a new tool for different applications in skin and cosmetic research.
Wendy Jessup - One of the best experts on this subject based on the ideXlab platform.
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visualizing Lipid Structure and raft domains in living cells with two photon microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2003Co-Authors: Katharina Gaus, Enrico Gratton, Eleanor P. W. Kable, Allan S. Jones, Ingrid C. Gelissen, Leonard Kritharides, Wendy JessupAbstract:The lateral organization of cellular membranes is formed by the clustering of specific Lipids, such as cholesterol and sphingoLipids, into highly condensed domains (termed Lipid rafts). Hence such domains are distinct from the remaining membrane by their Lipid Structure (liquid-ordered vs. -disordered domains). Here, we directly visualize membrane Lipid Structure of living cells by using two-photon microscopy. In macrophages, liquid-ordered domains are particularly enriched on membrane protrusions (filopodia), adhesion points and cell-cell contacts and cover 10-15% of the cell surface at 37 degrees C. By deconvoluting the images, we demonstrate the existence of phase separation in vivo. We compare the properties of microscopically visible domains (<1 microm2), with those of isolated detergent-resistant membranes and provide evidence that membrane coverage by Lipid rafts and their fluidity are principally governed by cholesterol content, thereby providing strong support for the Lipid raft hypothesis.
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Visualizing Lipid Structure and raft domains in living cells with two-photon microscopy
Proceedings of the National Academy of Sciences of the United States of America, 2003Co-Authors: Katharina Gaus, Enrico Gratton, Eleanor P. W. Kable, Allan S. Jones, Ingrid C. Gelissen, Leonard Kritharides, Wendy JessupAbstract:The lateral organization of cellular membranes is formed by the clustering of specific Lipids, such as cholesterol and sphingoLipids, into highly condensed domains (termed Lipid rafts). Hence such domains are distinct from the remaining membrane by their Lipid Structure (liquid-ordered vs. -disordered domains). Here, we directly visualize membrane Lipid Structure of living cells by using two-photon microscopy. In macrophages, liquid-ordered domains are particularly enriched on membrane protrusions (filopodia), adhesion points and cell-cell contacts and cover 10-15% of the cell surface at 37 degrees C. By deconvoluting the images, we demonstrate the existence of phase separation in vivo. We compare the properties of microscopically visible domains (
C C Mullergoymann - One of the best experts on this subject based on the ideXlab platform.
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the influence of topical formulations on the permeation of 5 aminolevulinic acid and its n butyl ester through excised human stratum corneum
European Journal of Pharmaceutics and Biopharmaceutics, 2005Co-Authors: Axel Winkler, C C MullergoymannAbstract:The limited permeation of 5-aminolevulinic acid (ALA) through excised human stratum corneum could be improved by using 5-aminolevulinic acid-n-butyl ester (ABE). Furthermore drug permeation could be increased by choice of a permeation enhancing formulation. In this study, permeation of ALA and ABE was investigated from various formulations. In addition, differential scanning calorimetry (DSC) and wide angle X-ray diffraction (WAXD) experiments were performed in order to reveal an interaction between the tested formulations and stratum corneum Lipid Structure. Drug incorporation into Dolgit Mikrogel showed the highest increase in permeability with both ALA and ABE. Especially, ABE together with Dolgit Mikrogel was the most promising combination. Further permeation studies with poloxamer based ABE formulations, partially enriched with ibuprofen acid and medium chained triglycerides showed that both compounds promote permeation. The permeation coefficients of either drug from Excipial Creme and Basiscreme DAC were found to be very similar. These results were in accordance with those of DSC and WAXD experiments. Interaction between formulation and stratum corneum Lipid Structure resulting in an increased drug permeation only occurred after pretreatment with formulations enriched with ibuprofen acid. After pretreatment with Excipial Creme, Basiscreme DAC or Excipial Fettcreme stratum corneum Structure and subsequently permeability remained unchanged. Nevertheless permeation of ALA from Excipial Fettcreme is slower than from the tested hydrophilic formulations and therefore believed to be influenced by the affinity of ALA to the vehicle and stratum corneum.