The Experts below are selected from a list of 1080 Experts worldwide ranked by ideXlab platform

Stephen P Newman - One of the best experts on this subject based on the ideXlab platform.

  • lung deposition of budesonide inhaled via turbuhaler a comparison with terbutaline sulphate in normal subjects
    European Respiratory Journal, 1994
    Co-Authors: Lars Borgstrom, Eva Bondesson, Folke Moren, Eva Annchristin Trofast, Stephen P Newman
    Abstract:

    We wanted to evaluate whether lung deposition of budesonide and terbutaline sulphate differs, and to determine lung deposition of budesonide inhaled at different peak inspiratory flows, through Turbuhaler. Lung deposition of budesonide, a Lipophilic Substance, and of terbutaline sulphate, a hydrophilic Substance, was therefore compared, after administration via an inspiratory flow-driven, multi-dose, powder inhaler (Turbuhaler, Astra Draco AB) to 10 healthy volunteers. The radionuclide 99mTc was used to label drug particles, and radioactivity, indicating drug deposition, was measured using a gamma camera. Budesonide was inhaled at a normal flow of 58 l.min-1 and at a slow flow of 36 l-min-1. At the faster flow, a mean +/- SD 27.7 +/- 9.5% of the metered dose was deposited in the lung and at the slower flow 14.8 +/- 3.3% was deposited (p

  • lung deposition of budesonide inhaled via turbuhaler a comparison with terbutaline sulphate in normal subjects
    European Respiratory Journal, 1994
    Co-Authors: Lars Borgstrom, Eva Bondesson, Folke Moren, Eva Annchristin Trofast, Stephen P Newman
    Abstract:

    We wanted to evaluate whether lung deposition of budesonide and terbutaline sulphate differs, and to determine lung deposition of budesonide inhaled at different peak inspiratory flows, through Turbuhaler. Lung deposition of budesonide, a Lipophilic Substance, and of terbutaline sulphate, a hydrophilic Substance, was therefore compared, after administration via an inspiratory flow-driven, multi-dose, powder inhaler (Turbuhaler, Astra Draco AB) to 10 healthy volunteers. The radionuclide 99mTc was used to label drug particles, and radioactivity, indicating drug deposition, was measured using a gamma camera. Budesonide was inhaled at a normal flow of 58 l.min-1 and at a slow flow of 36 l-min-1. At the faster flow, a mean +/- SD 27.7 +/- 9.5% of the metered dose was deposited in the lung and at the slower flow 14.8 +/- 3.3% was deposited (p < 0.001). Mean lung deposition of terbutaline sulphate inhaled at 57 l.min-1 was 27.0 +/- 7.7%. We conclude that inspiratory flow has an important effect on lung deposition, but water solubility appears to have no effect.

Lars Borgstrom - One of the best experts on this subject based on the ideXlab platform.

  • lung deposition of budesonide inhaled via turbuhaler a comparison with terbutaline sulphate in normal subjects
    European Respiratory Journal, 1994
    Co-Authors: Lars Borgstrom, Eva Bondesson, Folke Moren, Eva Annchristin Trofast, Stephen P Newman
    Abstract:

    We wanted to evaluate whether lung deposition of budesonide and terbutaline sulphate differs, and to determine lung deposition of budesonide inhaled at different peak inspiratory flows, through Turbuhaler. Lung deposition of budesonide, a Lipophilic Substance, and of terbutaline sulphate, a hydrophilic Substance, was therefore compared, after administration via an inspiratory flow-driven, multi-dose, powder inhaler (Turbuhaler, Astra Draco AB) to 10 healthy volunteers. The radionuclide 99mTc was used to label drug particles, and radioactivity, indicating drug deposition, was measured using a gamma camera. Budesonide was inhaled at a normal flow of 58 l.min-1 and at a slow flow of 36 l-min-1. At the faster flow, a mean +/- SD 27.7 +/- 9.5% of the metered dose was deposited in the lung and at the slower flow 14.8 +/- 3.3% was deposited (p

  • lung deposition of budesonide inhaled via turbuhaler a comparison with terbutaline sulphate in normal subjects
    European Respiratory Journal, 1994
    Co-Authors: Lars Borgstrom, Eva Bondesson, Folke Moren, Eva Annchristin Trofast, Stephen P Newman
    Abstract:

    We wanted to evaluate whether lung deposition of budesonide and terbutaline sulphate differs, and to determine lung deposition of budesonide inhaled at different peak inspiratory flows, through Turbuhaler. Lung deposition of budesonide, a Lipophilic Substance, and of terbutaline sulphate, a hydrophilic Substance, was therefore compared, after administration via an inspiratory flow-driven, multi-dose, powder inhaler (Turbuhaler, Astra Draco AB) to 10 healthy volunteers. The radionuclide 99mTc was used to label drug particles, and radioactivity, indicating drug deposition, was measured using a gamma camera. Budesonide was inhaled at a normal flow of 58 l.min-1 and at a slow flow of 36 l-min-1. At the faster flow, a mean +/- SD 27.7 +/- 9.5% of the metered dose was deposited in the lung and at the slower flow 14.8 +/- 3.3% was deposited (p < 0.001). Mean lung deposition of terbutaline sulphate inhaled at 57 l.min-1 was 27.0 +/- 7.7%. We conclude that inspiratory flow has an important effect on lung deposition, but water solubility appears to have no effect.

John S Ramsdell - One of the best experts on this subject based on the ideXlab platform.

  • Current Progress in Isolation and Characterization of Toxins Isolated from Pfiesteria piscicida
    2013
    Co-Authors: Peter D R Moeller, Jo Ann M. Burkholder, Nora J. Deamer-melia, Steve L. Morton, Brad A. Mitchell, Scott K. Sivertsen, Elizabeth R. Fairey, Tina M. Mikulski, Howard Glasgow, John S Ramsdell
    Abstract:

    The isolation and partial purification of toxic Substances derived from Pfiesteria piscicida Steidinger & Burkholder extracts is described. Four distinct bioassay systems were used to monitor bioactivity of the P. piscicida extracts, including a high throughput cell cytotoxicity assay and a reporter gene assay as well as assays using brine shrimp and fish. Using these bioassays to guide fractionation, we have isolated two distinct, active fractions from Pfiesteria culture medium and cell mass extracts on the basis of their solubility characteristics. We have identified and characterized a bioactive Lipophilic Substance from Pfiesteria-derived extracts as di(2-ethylhexyl)phthalate, a commonly used plasticizer. The source of this typically man-made Substance has been identified as originating from Instant Ocean (Aquarium Systems, Mentor, OH, USA), a commercially available seawater salt mixture used to prepare our mass culture growth medium. We have developed chromatographic methodology to isolate a bioactive polar compound isolated from extracts of Pfiesteria culture and presently report the characterization of the activity of this Substance. The molecular structural analysis of the polar active component(s) using mass spectrometry and nuclear magnetic resonance spectroscopy is currently under way. Key words: assay, chromatography, GH 4C 1, Pfiesteria piscicida, toxin bioassay. — Environ Health Perspect 109(suppl 5):739–743 (2001)

  • Current progress in isolation and characterization of toxins isolated from Pfiesteria piscicida.
    Environmental health perspectives, 2001
    Co-Authors: Peter D R Moeller, Jo Ann M. Burkholder, Howard B Glasgow, Nora J. Deamer-melia, Steve L. Morton, Brad A. Mitchell, Scott K. Sivertsen, Elizabeth R. Fairey, Tina M. Mikulski, John S Ramsdell
    Abstract:

    The isolation and partial purification of toxic Substances derived from Pfiesteria piscicida Steidinger & Burkholder extracts is described. Four distinct bioassay systems were used to monitor bioactivity of the P. piscicida extracts, including a high throughput cell cytotoxicity assay and a reporter gene assay as well as assays using brine shrimp and fish. Using these bioassays to guide fractionation, we have isolated two distinct, active fractions from Pfiesteria culture medium and cell mass extracts on the basis of their solubility characteristics. We have identified and characterized a bioactive Lipophilic Substance from Pfiesteria-derived extracts as di(2-ethylhexyl)phthalate, a commonly used plasticizer. The source of this typically man-made Substance has been identified as originating from Instant Ocean (Aquarium Systems, Mentor, OH, USA), a commercially available seawater salt mixture used to prepare our mass culture growth medium. We have developed chromatographic methodology to isolate a bioactive polar compound isolated from extracts of Pfiesteria culture and presently report the characterization of the activity of this Substance. The molecular structural analysis of the polar active component(s) using mass spectrometry and nuclear magnetic resonance spectroscopy is currently under way.

Eva Bondesson - One of the best experts on this subject based on the ideXlab platform.

  • lung deposition of budesonide inhaled via turbuhaler a comparison with terbutaline sulphate in normal subjects
    European Respiratory Journal, 1994
    Co-Authors: Lars Borgstrom, Eva Bondesson, Folke Moren, Eva Annchristin Trofast, Stephen P Newman
    Abstract:

    We wanted to evaluate whether lung deposition of budesonide and terbutaline sulphate differs, and to determine lung deposition of budesonide inhaled at different peak inspiratory flows, through Turbuhaler. Lung deposition of budesonide, a Lipophilic Substance, and of terbutaline sulphate, a hydrophilic Substance, was therefore compared, after administration via an inspiratory flow-driven, multi-dose, powder inhaler (Turbuhaler, Astra Draco AB) to 10 healthy volunteers. The radionuclide 99mTc was used to label drug particles, and radioactivity, indicating drug deposition, was measured using a gamma camera. Budesonide was inhaled at a normal flow of 58 l.min-1 and at a slow flow of 36 l-min-1. At the faster flow, a mean +/- SD 27.7 +/- 9.5% of the metered dose was deposited in the lung and at the slower flow 14.8 +/- 3.3% was deposited (p

  • lung deposition of budesonide inhaled via turbuhaler a comparison with terbutaline sulphate in normal subjects
    European Respiratory Journal, 1994
    Co-Authors: Lars Borgstrom, Eva Bondesson, Folke Moren, Eva Annchristin Trofast, Stephen P Newman
    Abstract:

    We wanted to evaluate whether lung deposition of budesonide and terbutaline sulphate differs, and to determine lung deposition of budesonide inhaled at different peak inspiratory flows, through Turbuhaler. Lung deposition of budesonide, a Lipophilic Substance, and of terbutaline sulphate, a hydrophilic Substance, was therefore compared, after administration via an inspiratory flow-driven, multi-dose, powder inhaler (Turbuhaler, Astra Draco AB) to 10 healthy volunteers. The radionuclide 99mTc was used to label drug particles, and radioactivity, indicating drug deposition, was measured using a gamma camera. Budesonide was inhaled at a normal flow of 58 l.min-1 and at a slow flow of 36 l-min-1. At the faster flow, a mean +/- SD 27.7 +/- 9.5% of the metered dose was deposited in the lung and at the slower flow 14.8 +/- 3.3% was deposited (p < 0.001). Mean lung deposition of terbutaline sulphate inhaled at 57 l.min-1 was 27.0 +/- 7.7%. We conclude that inspiratory flow has an important effect on lung deposition, but water solubility appears to have no effect.

Folke Moren - One of the best experts on this subject based on the ideXlab platform.

  • lung deposition of budesonide inhaled via turbuhaler a comparison with terbutaline sulphate in normal subjects
    European Respiratory Journal, 1994
    Co-Authors: Lars Borgstrom, Eva Bondesson, Folke Moren, Eva Annchristin Trofast, Stephen P Newman
    Abstract:

    We wanted to evaluate whether lung deposition of budesonide and terbutaline sulphate differs, and to determine lung deposition of budesonide inhaled at different peak inspiratory flows, through Turbuhaler. Lung deposition of budesonide, a Lipophilic Substance, and of terbutaline sulphate, a hydrophilic Substance, was therefore compared, after administration via an inspiratory flow-driven, multi-dose, powder inhaler (Turbuhaler, Astra Draco AB) to 10 healthy volunteers. The radionuclide 99mTc was used to label drug particles, and radioactivity, indicating drug deposition, was measured using a gamma camera. Budesonide was inhaled at a normal flow of 58 l.min-1 and at a slow flow of 36 l-min-1. At the faster flow, a mean +/- SD 27.7 +/- 9.5% of the metered dose was deposited in the lung and at the slower flow 14.8 +/- 3.3% was deposited (p

  • lung deposition of budesonide inhaled via turbuhaler a comparison with terbutaline sulphate in normal subjects
    European Respiratory Journal, 1994
    Co-Authors: Lars Borgstrom, Eva Bondesson, Folke Moren, Eva Annchristin Trofast, Stephen P Newman
    Abstract:

    We wanted to evaluate whether lung deposition of budesonide and terbutaline sulphate differs, and to determine lung deposition of budesonide inhaled at different peak inspiratory flows, through Turbuhaler. Lung deposition of budesonide, a Lipophilic Substance, and of terbutaline sulphate, a hydrophilic Substance, was therefore compared, after administration via an inspiratory flow-driven, multi-dose, powder inhaler (Turbuhaler, Astra Draco AB) to 10 healthy volunteers. The radionuclide 99mTc was used to label drug particles, and radioactivity, indicating drug deposition, was measured using a gamma camera. Budesonide was inhaled at a normal flow of 58 l.min-1 and at a slow flow of 36 l-min-1. At the faster flow, a mean +/- SD 27.7 +/- 9.5% of the metered dose was deposited in the lung and at the slower flow 14.8 +/- 3.3% was deposited (p < 0.001). Mean lung deposition of terbutaline sulphate inhaled at 57 l.min-1 was 27.0 +/- 7.7%. We conclude that inspiratory flow has an important effect on lung deposition, but water solubility appears to have no effect.