The Experts below are selected from a list of 117528 Experts worldwide ranked by ideXlab platform
Nigel P Brunton - One of the best experts on this subject based on the ideXlab platform.
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Recovery of steroidal alkaloids from potato peels using pressurized Liquid Extraction.
Molecules, 2015Co-Authors: Mohammad B Hossain, Ashish Rawson, Ingrid Aguiló-aguayo, Nigel P BruntonAbstract:A higher yield of glycoalkaloids was recovered from potato peels using pressurized Liquid Extraction (1.92 mg/g dried potato peels) compared to conventional solid–Liquid Extraction (0.981 mg/g dried potato peels). Response surface methodology deduced the optimal temperature and extracting solvent (methanol) for the pressurized Liquid Extraction (PLE) of glycoalkaloids as 80 °C in 89% methanol. Using these two optimum PLE conditions, levels of individual steroidal alkaloids obtained were of 597, 873, 374 and 75 µg/g dried potato peel for α-solanine, α-chaconine, solanidine and demissidine respectively. Corresponding values for solid Liquid Extraction were 59%, 46%, 40% and 52% lower for α-solanine, α-chaconine, solanidine and demissidine respectively.
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Influence of pressurised Liquid Extraction and solid–Liquid Extraction methods on the phenolic content and antioxidant activities of Irish macroalgae
International Journal of Food Science and Technology, 2012Co-Authors: Michelle S Tierney, Anna Soler-vila, Maria Hayes, Anna K Croft, Thomas J Smyth, Nigel P BruntonAbstract:Summary The efficiencies of pressurised Liquid Extraction (PLE) and a traditional solid–Liquid Extraction (SLE) at extracting antioxidant polyphenols from Irish macroalgae Ascophyllum nodosum, Pelvetia canaliculata, Fucus spiralis and Ulva intestinalis were compared. PLE was more effective for extracting polyphenols with acetone/water (80:20); however, when food-friendly solvents of ethanol/water (80:20) and water were employed, SLE resulted in higher phenolic content in brown macroalgal extracts. For example, the Fucus spiralis SLE water and ethanol/water extracts displayed total phenolic contents (TPCs) of 130.58 ± 2.78 and 142.81 ± 1.77 μg phloroglucinol equivalents (PGE) mg−1 sample, respectively, compared with TPCs of 90.79 ± 1.16 and 124 ± 6.54 μg PGE mg−1 sample for the corresponding PLE extracts. All SLE aqueous ethanolic macroalgal extracts possessed higher DPPH radical scavenging abilities (RSA) and ferric reducing antioxidant power (FRAP) than their PLE equivalents. This study indicates that the application of high Extraction temperatures (50–200 °C) and pressures (500–3000 psi) used in PLE does not enhance the antioxidant activities of macroalgal extracts relative to SLE Extraction. The ability to produce antioxidant food-friendly macroalgal extracts using SLE could represent significant cost reductions on an industrial scale further enhancing the potential of macroalgal polyphenols to be used in functional food preparations.
T. Alan Hatton - One of the best experts on this subject based on the ideXlab platform.
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Protein refolding by reversed micelles utilizing solid-Liquid Extraction technique.
Biotechnology and bioengineering, 1998Co-Authors: Yukihisa Hashimoto, Tsutomu Ono, Masahiro Goto, T. Alan HattonAbstract:This article reports that a reversed micellar solution is useful for refolding proteins directly from a solid source. The solubilization of denatured RNase A, which had been prepared by reprecipitation from the denaturant protein solution, into reversed micelles formulated with sodium di-2-ethylhexyl sulfosuccinate (AOT) has been investigated by a solid-Liquid Extraction system. This method is an alternative to the ordinary protein Extraction in reversed micelles based on the Liquid-Liquid Extraction. The solid-Liquid Extraction method was found to facilitate the solubilization of denatured proteins more efficiently in the reversed micellar media than the ordinary phase transfer method of Liquid Extraction. The refolding of denatured RNase A entrapped in reversed micelles was attained by adding a redox reagent (reduced and oxidized glutathion). Enzymatic activity of RNase A was gradually recovered with time in the reversed micelles. The denatured RNase A was completely refolded within 30 h. In addition, the efficiency of protein refolding was enhanced when reversed micelles were applied to denatured RNase A containing a higher protein concentration that, in the case of aqueous media, would lead to protein aggregation. The solid-Liquid Extraction technique using reversed micelles affords better scale-up advantages in the direct refolding process of insoluble protein aggregates.
Mohammad B Hossain - One of the best experts on this subject based on the ideXlab platform.
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Recovery of steroidal alkaloids from potato peels using pressurized Liquid Extraction.
Molecules, 2015Co-Authors: Mohammad B Hossain, Ashish Rawson, Ingrid Aguiló-aguayo, Nigel P BruntonAbstract:A higher yield of glycoalkaloids was recovered from potato peels using pressurized Liquid Extraction (1.92 mg/g dried potato peels) compared to conventional solid–Liquid Extraction (0.981 mg/g dried potato peels). Response surface methodology deduced the optimal temperature and extracting solvent (methanol) for the pressurized Liquid Extraction (PLE) of glycoalkaloids as 80 °C in 89% methanol. Using these two optimum PLE conditions, levels of individual steroidal alkaloids obtained were of 597, 873, 374 and 75 µg/g dried potato peel for α-solanine, α-chaconine, solanidine and demissidine respectively. Corresponding values for solid Liquid Extraction were 59%, 46%, 40% and 52% lower for α-solanine, α-chaconine, solanidine and demissidine respectively.
Ji Chen - One of the best experts on this subject based on the ideXlab platform.
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the solid Liquid Extraction of yttrium from rare earths by solvent ionic Liquid impreganated resin coupled with complexing method
Separation and Purification Technology, 2008Co-Authors: Xiaoqi Sun, Bo Peng, Ji ChenAbstract:Abstract A kind of solvent (ionic Liquid) impreganated resin (IL-SIR) was developed herein for ameliorating imidazolium-type IL-based Liquid–Liquid Extraction of metal ions. In this study, [C 8 mim][PF 6 ] containing Cyanex923 was immobilized on XAD-7 resin for solid–Liquid Extraction of rare earth (RE). The solid–Liquid Extraction contributed to ameliorating mass transfer efficiency, i.e. shortening equilibrium time from 40 min to 20 min, increasing Extraction efficiency from 29% to 80%. In additional, the novel IL-SIR could separate Y(III) from Sc(III), Ho(III), Er(III), Yb(III) effectively by adding water-soluble complexing agent. Thus, the solid–Liquid Extraction by IL-SIR coupled with complexing method could be regarded as an effective strategy for improving mass transfer efficiency and increasing selectivity of IL-based Liquid–Liquid Extraction.
Yukihisa Hashimoto - One of the best experts on this subject based on the ideXlab platform.
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Protein refolding by reversed micelles utilizing solid-Liquid Extraction technique.
Biotechnology and bioengineering, 1998Co-Authors: Yukihisa Hashimoto, Tsutomu Ono, Masahiro Goto, T. Alan HattonAbstract:This article reports that a reversed micellar solution is useful for refolding proteins directly from a solid source. The solubilization of denatured RNase A, which had been prepared by reprecipitation from the denaturant protein solution, into reversed micelles formulated with sodium di-2-ethylhexyl sulfosuccinate (AOT) has been investigated by a solid-Liquid Extraction system. This method is an alternative to the ordinary protein Extraction in reversed micelles based on the Liquid-Liquid Extraction. The solid-Liquid Extraction method was found to facilitate the solubilization of denatured proteins more efficiently in the reversed micellar media than the ordinary phase transfer method of Liquid Extraction. The refolding of denatured RNase A entrapped in reversed micelles was attained by adding a redox reagent (reduced and oxidized glutathion). Enzymatic activity of RNase A was gradually recovered with time in the reversed micelles. The denatured RNase A was completely refolded within 30 h. In addition, the efficiency of protein refolding was enhanced when reversed micelles were applied to denatured RNase A containing a higher protein concentration that, in the case of aqueous media, would lead to protein aggregation. The solid-Liquid Extraction technique using reversed micelles affords better scale-up advantages in the direct refolding process of insoluble protein aggregates.
