The Experts below are selected from a list of 108738 Experts worldwide ranked by ideXlab platform
Youlin Qiao - One of the best experts on this subject based on the ideXlab platform.
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an evaluation of clinical performance of fta cards for hpv 16 18 detection using cobas 4800 hpv test compared to dry swab and Liquid Medium
Journal of Clinical Virology, 2017Co-Authors: Li Dong, Li Li, Margaret Z Wang, Ruimei Feng, Zeni Wu, Jia Lian, Guangdong Liao, Wen Chen, Youlin QiaoAbstract:Abstract Backgrounds Effective dry storage and transport media as an alternative to conventional Liquid-based Medium would facilitate the accessibility of women in the low-resource settings to human papillomavirus (HPV)- based cervical cancer screening. Objective To evaluate analytical and clinical performance of indicating FTA™ Elute Cartridge (FTA card) for the detection of HPV16/18 and cervical precancerous lesions and cancer compared to dry swab and Liquid Medium. Study design Ninety patients with abnormal cytology and/or HPV infection were included for analysis. Three specimens of cervical exfoliated cells from each woman were randomly collected by FTA card, dry swab or Liquid-based Medium prior to colposcopy examination. The subsequent HPV DNA tests were performed on cobas 4800 HPV platform. Results and conclusions High-risk HPV (hrHPV) positivity rate was 63.3%, 62.2% and 65.6% for samples collected by FTA card, dry swab and Liquid Medium, respectively. The overall agreements and kappa values for the detection of hrHPV, HPV 16 and HPV 18 between FTA card and Liquid-based Medium were 88.9% (κ = 0.76), 97.8% (κ = 0.94) and 100% (κ = 1.0),respectively; between FTA card and dry swab were 92.1% (κ = 0.83), 94.5% (κ = 0.87) and 100% (κ = 1.0), respectively. The performances of hrHPV tested by FTA card, dry swab, and Liquid-based Medium for detecting CIN2+ were comparable in terms of the sensitivity and specificity. The specificity of detection of CIN2+ by HPV16/18 increased by approximately 40% compared to hrHPV for any Medium albeit at cost of a moderate loss of sensitivity. Dry Medium might offer an alternative to conventional Liquid-based Medium in the HPV-based cervical cancer screening program especially in low-resource settings but still needs further evaluation.
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an evaluation of clinical performance of fta cards for hpv 16 18 detection using cobas 4800 hpv test compared to dry swab and Liquid Medium
Journal of Clinical Virology, 2017Co-Authors: Li Dong, Margaret Z Wang, Ruimei Feng, Jia Lian, Guangdong Liao, Wen Chen, Chunqing Lin, Jianfeng Cui, Bin Liu, Youlin QiaoAbstract:Abstract Backgrounds Effective dry storage and transport media as an alternative to conventional Liquid-based Medium would facilitate the accessibility of women in the low-resource settings to human papillomavirus (HPV)- based cervical cancer screening. Objective To evaluate analytical and clinical performance of indicating FTA™ Elute Cartridge (FTA card) for the detection of HPV16/18 and cervical precancerous lesions and cancer compared to dry swab and Liquid Medium. Study design Ninety patients with abnormal cytology and/or HPV infection were included for analysis. Three specimens of cervical exfoliated cells from each woman were randomly collected by FTA card, dry swab or Liquid-based Medium prior to colposcopy examination. The subsequent HPV DNA tests were performed on cobas 4800 HPV platform. Results and conclusions High-risk HPV (hrHPV) positivity rate was 63.3%, 62.2% and 65.6% for samples collected by FTA card, dry swab and Liquid Medium, respectively. The overall agreements and kappa values for the detection of hrHPV, HPV 16 and HPV 18 between FTA card and Liquid-based Medium were 88.9% (κ = 0.76), 97.8% (κ = 0.94) and 100% (κ = 1.0),respectively; between FTA card and dry swab were 92.1% (κ = 0.83), 94.5% (κ = 0.87) and 100% (κ = 1.0), respectively. The performances of hrHPV tested by FTA card, dry swab, and Liquid-based Medium for detecting CIN2+ were comparable in terms of the sensitivity and specificity. The specificity of detection of CIN2+ by HPV16/18 increased by approximately 40% compared to hrHPV for any Medium albeit at cost of a moderate loss of sensitivity. Dry Medium might offer an alternative to conventional Liquid-based Medium in the HPV-based cervical cancer screening program especially in low-resource settings but still needs further evaluation.
Li Dong - One of the best experts on this subject based on the ideXlab platform.
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an evaluation of clinical performance of fta cards for hpv 16 18 detection using cobas 4800 hpv test compared to dry swab and Liquid Medium
Journal of Clinical Virology, 2017Co-Authors: Li Dong, Li Li, Margaret Z Wang, Ruimei Feng, Zeni Wu, Jia Lian, Guangdong Liao, Wen Chen, Youlin QiaoAbstract:Abstract Backgrounds Effective dry storage and transport media as an alternative to conventional Liquid-based Medium would facilitate the accessibility of women in the low-resource settings to human papillomavirus (HPV)- based cervical cancer screening. Objective To evaluate analytical and clinical performance of indicating FTA™ Elute Cartridge (FTA card) for the detection of HPV16/18 and cervical precancerous lesions and cancer compared to dry swab and Liquid Medium. Study design Ninety patients with abnormal cytology and/or HPV infection were included for analysis. Three specimens of cervical exfoliated cells from each woman were randomly collected by FTA card, dry swab or Liquid-based Medium prior to colposcopy examination. The subsequent HPV DNA tests were performed on cobas 4800 HPV platform. Results and conclusions High-risk HPV (hrHPV) positivity rate was 63.3%, 62.2% and 65.6% for samples collected by FTA card, dry swab and Liquid Medium, respectively. The overall agreements and kappa values for the detection of hrHPV, HPV 16 and HPV 18 between FTA card and Liquid-based Medium were 88.9% (κ = 0.76), 97.8% (κ = 0.94) and 100% (κ = 1.0),respectively; between FTA card and dry swab were 92.1% (κ = 0.83), 94.5% (κ = 0.87) and 100% (κ = 1.0), respectively. The performances of hrHPV tested by FTA card, dry swab, and Liquid-based Medium for detecting CIN2+ were comparable in terms of the sensitivity and specificity. The specificity of detection of CIN2+ by HPV16/18 increased by approximately 40% compared to hrHPV for any Medium albeit at cost of a moderate loss of sensitivity. Dry Medium might offer an alternative to conventional Liquid-based Medium in the HPV-based cervical cancer screening program especially in low-resource settings but still needs further evaluation.
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an evaluation of clinical performance of fta cards for hpv 16 18 detection using cobas 4800 hpv test compared to dry swab and Liquid Medium
Journal of Clinical Virology, 2017Co-Authors: Li Dong, Margaret Z Wang, Ruimei Feng, Jia Lian, Guangdong Liao, Wen Chen, Chunqing Lin, Jianfeng Cui, Bin Liu, Youlin QiaoAbstract:Abstract Backgrounds Effective dry storage and transport media as an alternative to conventional Liquid-based Medium would facilitate the accessibility of women in the low-resource settings to human papillomavirus (HPV)- based cervical cancer screening. Objective To evaluate analytical and clinical performance of indicating FTA™ Elute Cartridge (FTA card) for the detection of HPV16/18 and cervical precancerous lesions and cancer compared to dry swab and Liquid Medium. Study design Ninety patients with abnormal cytology and/or HPV infection were included for analysis. Three specimens of cervical exfoliated cells from each woman were randomly collected by FTA card, dry swab or Liquid-based Medium prior to colposcopy examination. The subsequent HPV DNA tests were performed on cobas 4800 HPV platform. Results and conclusions High-risk HPV (hrHPV) positivity rate was 63.3%, 62.2% and 65.6% for samples collected by FTA card, dry swab and Liquid Medium, respectively. The overall agreements and kappa values for the detection of hrHPV, HPV 16 and HPV 18 between FTA card and Liquid-based Medium were 88.9% (κ = 0.76), 97.8% (κ = 0.94) and 100% (κ = 1.0),respectively; between FTA card and dry swab were 92.1% (κ = 0.83), 94.5% (κ = 0.87) and 100% (κ = 1.0), respectively. The performances of hrHPV tested by FTA card, dry swab, and Liquid-based Medium for detecting CIN2+ were comparable in terms of the sensitivity and specificity. The specificity of detection of CIN2+ by HPV16/18 increased by approximately 40% compared to hrHPV for any Medium albeit at cost of a moderate loss of sensitivity. Dry Medium might offer an alternative to conventional Liquid-based Medium in the HPV-based cervical cancer screening program especially in low-resource settings but still needs further evaluation.
N. F. Bakeev - One of the best experts on this subject based on the ideXlab platform.
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the structural evolution of high density polyethylene during crazing in Liquid Medium
European Polymer Journal, 2015Co-Authors: Alena Yu Yarysheva, Dmitry V. Bagrov, E. G. Rukhlya, A. L. Volynskii, L. M. Yarysheva, N. F. BakeevAbstract:Abstract Atomic force microscopy (AFM) was employed to study structural transformations occurring in high-density polyethylene (HDPE) during deformation in a Liquid Medium by crazing mechanism. Processing of the obtained images yielded the parameters of HDPE structure at different tensile strains. It was shown that crazing causes the development of a fibrillar–porous structure in the interlamellar space, the fragmentation of lamellae, and the displacement of lamella fragments relative to each other. Moreover, the deformation is accompanied by the separation of lamellae and the long period increases in the proportion to the tensile strain. The scheme of HDPE deformation upon crazing in Liquid Medium was constructed based on the AFM images.
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atomic force microscopic study of the structure of high density polyethylene deformed in Liquid Medium by crazing mechanism
Journal of Microscopy, 2014Co-Authors: Dmitry V. Bagrov, Alena Yu Yarysheva, E. G. Rukhlya, A. L. Volynskii, L. M. Yarysheva, N. F. BakeevAbstract:Summary A procedure has been developed for the direct atomic force microscopic (AFM) examination of the native structure of high-density polyethylene (HDPE) deformed in an adsorption-active Liquid Medium (AALM) by the crazing mechanism. The AFM investigation has been carried out in the presence of a Liquid Medium under conditions preventing deformed films from shrinkage. Deformation of HDPE in AALM has been shown to proceed through the delocalized crazing mechanism and result in the development of a fibrillar-porous structure. The structural parameters of the crazed polymer have been determined. The obtained AFM images demonstrate a nanosized nonuniformity of the deformation and enable one to observe the structural rearrangements that take place in the deformed polymer after removal of the Liquid Medium and stress relaxation. A structural similarity has been revealed between HDPE deformed in the AALM and hard elastic polymers.
Dmitry V. Bagrov - One of the best experts on this subject based on the ideXlab platform.
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effect of initial polypropylene structure on its deformation via crazing mechanism in a Liquid Medium
European Polymer Journal, 2018Co-Authors: Alena Yu Yarysheva, Dmitry V. Bagrov, L. M. Yarysheva, Artem V Bakirov, S N Chvalun, A. L. VolynskiiAbstract:Abstract Atomic force microscopy has been employed to study the structure of isotactic polypropylene (PP) deformed in a physically active Liquid Medium (PALM) by the crazing mechanism. The investigations have been performed directly in the Liquid, in which deformation is carried out, and under conditions excluding PP contraction. In order to study the effect of the initial PP structure on the crazing mechanism, the polymer structure was varied by annealing. The crazing mechanism, as well as the parameters and morphology of the polymer, has been investigated at different tensile strain values as depending on the initial PP structure. It has been shown that the development of deformation via the classical or intercrystallite crazing mechanisms is predetermined by the degree of crystallinity and the thickness of lamellae in initial PP. Before annealing, PP is deformed via the classical mechanism, i.e. as a uniform one-phase material, with the formation of crazes and zones of the bulk polymer located between them. Because the lamellae are «weak», the lamellar structure is transformed into a fibrillar structure, with porosity developing in crazes between fibrillar strands. After annealing of PP, the thickness of lamellae, degree of crystallinity, and, accordingly, the drag of crystallites increase, cavitations arise in the amorphous phase, and the fibrillar-porous structure develops in the space between crystallites moving apart from each other.
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the structural evolution of high density polyethylene during crazing in Liquid Medium
European Polymer Journal, 2015Co-Authors: Alena Yu Yarysheva, Dmitry V. Bagrov, E. G. Rukhlya, A. L. Volynskii, L. M. Yarysheva, N. F. BakeevAbstract:Abstract Atomic force microscopy (AFM) was employed to study structural transformations occurring in high-density polyethylene (HDPE) during deformation in a Liquid Medium by crazing mechanism. Processing of the obtained images yielded the parameters of HDPE structure at different tensile strains. It was shown that crazing causes the development of a fibrillar–porous structure in the interlamellar space, the fragmentation of lamellae, and the displacement of lamella fragments relative to each other. Moreover, the deformation is accompanied by the separation of lamellae and the long period increases in the proportion to the tensile strain. The scheme of HDPE deformation upon crazing in Liquid Medium was constructed based on the AFM images.
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atomic force microscopic study of the structure of high density polyethylene deformed in Liquid Medium by crazing mechanism
Journal of Microscopy, 2014Co-Authors: Dmitry V. Bagrov, Alena Yu Yarysheva, E. G. Rukhlya, A. L. Volynskii, L. M. Yarysheva, N. F. BakeevAbstract:Summary A procedure has been developed for the direct atomic force microscopic (AFM) examination of the native structure of high-density polyethylene (HDPE) deformed in an adsorption-active Liquid Medium (AALM) by the crazing mechanism. The AFM investigation has been carried out in the presence of a Liquid Medium under conditions preventing deformed films from shrinkage. Deformation of HDPE in AALM has been shown to proceed through the delocalized crazing mechanism and result in the development of a fibrillar-porous structure. The structural parameters of the crazed polymer have been determined. The obtained AFM images demonstrate a nanosized nonuniformity of the deformation and enable one to observe the structural rearrangements that take place in the deformed polymer after removal of the Liquid Medium and stress relaxation. A structural similarity has been revealed between HDPE deformed in the AALM and hard elastic polymers.
Alena Yu Yarysheva - One of the best experts on this subject based on the ideXlab platform.
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effect of initial polypropylene structure on its deformation via crazing mechanism in a Liquid Medium
European Polymer Journal, 2018Co-Authors: Alena Yu Yarysheva, Dmitry V. Bagrov, L. M. Yarysheva, Artem V Bakirov, S N Chvalun, A. L. VolynskiiAbstract:Abstract Atomic force microscopy has been employed to study the structure of isotactic polypropylene (PP) deformed in a physically active Liquid Medium (PALM) by the crazing mechanism. The investigations have been performed directly in the Liquid, in which deformation is carried out, and under conditions excluding PP contraction. In order to study the effect of the initial PP structure on the crazing mechanism, the polymer structure was varied by annealing. The crazing mechanism, as well as the parameters and morphology of the polymer, has been investigated at different tensile strain values as depending on the initial PP structure. It has been shown that the development of deformation via the classical or intercrystallite crazing mechanisms is predetermined by the degree of crystallinity and the thickness of lamellae in initial PP. Before annealing, PP is deformed via the classical mechanism, i.e. as a uniform one-phase material, with the formation of crazes and zones of the bulk polymer located between them. Because the lamellae are «weak», the lamellar structure is transformed into a fibrillar structure, with porosity developing in crazes between fibrillar strands. After annealing of PP, the thickness of lamellae, degree of crystallinity, and, accordingly, the drag of crystallites increase, cavitations arise in the amorphous phase, and the fibrillar-porous structure develops in the space between crystallites moving apart from each other.
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the structural evolution of high density polyethylene during crazing in Liquid Medium
European Polymer Journal, 2015Co-Authors: Alena Yu Yarysheva, Dmitry V. Bagrov, E. G. Rukhlya, A. L. Volynskii, L. M. Yarysheva, N. F. BakeevAbstract:Abstract Atomic force microscopy (AFM) was employed to study structural transformations occurring in high-density polyethylene (HDPE) during deformation in a Liquid Medium by crazing mechanism. Processing of the obtained images yielded the parameters of HDPE structure at different tensile strains. It was shown that crazing causes the development of a fibrillar–porous structure in the interlamellar space, the fragmentation of lamellae, and the displacement of lamella fragments relative to each other. Moreover, the deformation is accompanied by the separation of lamellae and the long period increases in the proportion to the tensile strain. The scheme of HDPE deformation upon crazing in Liquid Medium was constructed based on the AFM images.
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atomic force microscopic study of the structure of high density polyethylene deformed in Liquid Medium by crazing mechanism
Journal of Microscopy, 2014Co-Authors: Dmitry V. Bagrov, Alena Yu Yarysheva, E. G. Rukhlya, A. L. Volynskii, L. M. Yarysheva, N. F. BakeevAbstract:Summary A procedure has been developed for the direct atomic force microscopic (AFM) examination of the native structure of high-density polyethylene (HDPE) deformed in an adsorption-active Liquid Medium (AALM) by the crazing mechanism. The AFM investigation has been carried out in the presence of a Liquid Medium under conditions preventing deformed films from shrinkage. Deformation of HDPE in AALM has been shown to proceed through the delocalized crazing mechanism and result in the development of a fibrillar-porous structure. The structural parameters of the crazed polymer have been determined. The obtained AFM images demonstrate a nanosized nonuniformity of the deformation and enable one to observe the structural rearrangements that take place in the deformed polymer after removal of the Liquid Medium and stress relaxation. A structural similarity has been revealed between HDPE deformed in the AALM and hard elastic polymers.
