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Jay D King - One of the best experts on this subject based on the ideXlab platform.
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host defense peptides from Lithobates forreri hylarana luctuosa and hylarana signata ranidae phylogenetic relationships inferred from primary structures of ranatuerin 2 and brevinin 2 peptides
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2014Co-Authors: Michael J Conlon, Laurent Coquet, Thierry Jouenne, Jerome Leprince, Hubert Vaudry, Jolanta Kolodziejek, Norbert Nowotny, Milena Mechkarska, Per F Nielsen, Jay D KingAbstract:The primary structures of host-defense peptides present in frog skin secretions constitute useful molecular markers for establishing taxonomic classifications and investigating phylogenetic relationships between species within a particular genus. Peptidomic analysis has led to the characterization of multiple host-defense peptides in norepinephrine-stimulated skin secretions of three species of frogs from the family Ranidae: Lithobates forreri (Boulenger, 1883), Hylarana luctuosa (Peters, 1871), and Hylarana signata (Gunther, 1872). The L. forreri secretions contain ranatuerin-2 (2 peptides), brevinin-1 (4 peptides), and temporin (1 peptide). The H. luctuosa secretions contain brevinin-2 (4 peptides), esculentin-1 (1 peptide), esculentin-2 (1 peptide), palustrin-2 (2 peptides), and temporin (2 peptides). The H. signata secretions contain brevinin-2 (4 peptides), brevinin-1 (5 peptides), palustrin-2 (1 peptide), and temporin (2 peptides). Cladistic analysis based upon the primary structures of 44 ranatuerin-2 peptides from 20 Lithobates species indicates a close phylogenetic relationship between L. forreri, Lithobates onca, and Lithobates yavapaiensis. A similar cladistic analysis based upon the primary structures of 27 brevinin-2 peptides from 8 Hylarana species provides support for a close phylogenetic relationship between H. signata and Hylarana picturata, while showing that the species are not conspecific, with H. luctuosa more distantly related.
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Primary structures of skin antimicrobial peptides indicate a close, but not conspecific, phylogenetic relationship between the leopard frogs Lithobates onca and Lithobates yavapaiensis (Ranidae).
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2009Co-Authors: J M Conlon, Laurent Coquet, Thierry Jouenne, Jerome Leprince, Hubert Vaudry, Jay D KingAbstract:Abstract The phylogenetic relationship between the relict leopard frog Lithobates (Rana) onca (Cope, 1875) and the lowland leopard frog Lithobates (Rana) yavapaiensis (Platz and Frost, 1984) is unclear. Chromatographic analysis of norepinephrine-stimulated skin secretions from L. onca led to the identification of six peptides with antimicrobial activity. Determination of their primary structures indicated that four of the peptides were identical to brevinin-1Ya, brevinin-1Yb, brevinin-1Yc and ranatuerin-2Ya previously isolated from skin secretions of L. yavapaiensis. However, a peptide belonging to the temporin family (temporin-ONa: FLPTFGKILSGLF.NH2) and an atypical member of the ranatuerin-2 family containing a C-terminal cyclic heptapeptide domain (ranatuerin-2ONa: GLMDTVKNAAKNLAGQMLDKLKCKITGSC) were isolated from the L. onca secretions but were not present in the L. yavapaiensis secretions. Ranatuerin-2ONa inhibited the growth of Escherichia coli (MIC = 50 μM) and Candida albicans (MIC = 100 μM ) and showed hemolytic activity (LC50 = 90 μM) but was inactive against Staphylococcus aureus. The data indicate a close phylogenetic relationship between L. onca and L. yavapaiensis but suggest that they are not conspecific species.
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antimicrobial peptides from the skin secretions of the new world frogs Lithobates capito and Lithobates warszewitschii ranidae
Peptides, 2009Co-Authors: Michael J Conlon, Mohammed A Meetani, Laurent Coquet, Thierry Jouenne, Jerome Leprince, Hubert Vaudry, Jolanta Kolodziejek, Norbert Nowotny, Jay D KingAbstract:Abstract Taxonomic revisions within the anuran family Ranidae have established the genus Lithobates that currently comprises 49 species of frogs from the New World. Peptidomic analysis, using reversed-phase HPLC with on-line detection by electrospray mass spectrometry, has led to the identification of multiple antimicrobial peptides in norepinephrine-stimulated skin secretions of the North American frog Lithobates capito and the Central American frog Lithobates warszewitschii . Structural characterization of the peptides demonstrated that the L. capito secretions contained brevinin-1 (1), esculentin-1 (1), esculentin-2 (1), ranatuerin-2 (3), and temporin (2) peptides. L. warszewitschii secretions contained brevinin-1 (1), esculentin-2 (1), ranatuerin-2 (2), and temporin (1) peptides. Values in parentheses indicate number of peptides in each family. Temporin-CPa from L. capito , with the atypical structure IPPFIKKVLTTVF·NH 2 , also showed atypical growth-inhibitory activity having greater potency against Escherichia coli (MIC = 25 μM) and Candida albicans (MIC = 25 μM) than against Staphylococcus aureus (MIC = 50 μM). Phylogenetic analysis based upon the amino acid sequences of 37 ranatuerin-2 peptides from 17 species belonging to the genus Lithobates provides support for currently accepted taxonomic relationships. L. capito is sister-group to Lithobates sevosus in a clade that also contains Lithobates areolatus , and Lithobates palustris . L. warszewitschii is most closely related to the Central American species Lithobates tarahumarae and Lithobates vaillanti .
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peptides with potent cytolytic activity from the skin secretions of the north american leopard frogs Lithobates blairi and Lithobates yavapaiensis
Toxicon, 2009Co-Authors: J M Conlon, Laurent Coquet, Thierry Jouenne, Jerome Leprince, Hubert Vaudry, Eman Ahmed, Jay D KingAbstract:Abstract Six structurally similar and strongly cationic peptides belonging to the brevinin-1 family were isolated from skin secretions of the plains leopard frog Lithobates blairi and the lowland leopard frog Lithobates yavapaiensis on the basis of their antimicrobial activities. Brevinin-1BLc (FLPIIAGIAAKFLPKIFCTISKKC) from L. blairi represented the most potent peptide (MIC = 25 μM Escherichia coli , MIC = 1.5 μM Staphylococcus aureus , MIC = 3 μM Candida albicans , LC 50 = 9 μM human erythrocytes and LC 50 = 6 μM HepG2 human hepatoma-derived cells). The appreciably lower antimicrobial potencies of brevinin-1Ya and -1Yc from L. yavapaiensis correlate with the decreases in cationicity produced by the amino acid substitutions Lys 11 → Asn (brevinin-1Ya) and Pro 14 → Glu (brevinin-1Yc). In addition, a peptide isolated from the skin secretions of L. yavapaiensis belonging to the ranatuerin-2 family (ranatuerin-2Ya; GLMDTIKGVAKTVAASWLDKLKCKIT GC) inhibited the growth of E. coli (MIC = 50 μM) and S. aureus (MIC = 50 μM). In contrast to brevinin-1BLc, ranatuerin-2Ya showed appreciably greater cytolytic activity against HepG2 cells (LC 50 = 20 μM) than against erythrocytes (LC 50 > 100 μM).
Gabriel Laufer - One of the best experts on this subject based on the ideXlab platform.
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una nueva poblacion feral de rana toro Lithobates catesbeianus en uruguay encontrada con participacion ciudadana
Revista Latinoamericana de Herpetología, 2018Co-Authors: Gabriel Laufer, Noelia Gobel, Nadia Kacevas, Ignacio LadoAbstract:This note reports the occurrence of a new feral american bullfrog, Lithobates cathesbeianus, population in the locality of Cerrillos, Canelones Department, Uruguay. This population is within a protected area of great national importance. We recommend evaluating its status and distribution.
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Current status of American bullfrog, Lithobates catesbeianus, invasion in Uruguay and exploration of chytrid infection
Biological Invasions, 2018Co-Authors: Gabriel Laufer, Noelia Gobel, Claudio Borteiro, Alvaro Soutullo, Claudio Martínez-debatAbstract:The American bullfrog Lithobates catesbeianus is an invasive species that can strongly affect native amphibian communities through competition, predation, or introduction of diseases. This frog has invaded multiple areas in South America, for which niche models predict suitable environments across much of the continent. This paper reveals the state of the invasion of this species in Uruguay and its possible relationship with the chytrid pathogenic fungus, Batrachochytrium dendrobatidis . Surveys at invaded sites were conducted from 2007 to 2015, identified two populations undergoing recent range expansion (one of them exponential), two populations that failed to establish, and a new record in an urban area of the capital city, Montevideo. In all the analysed feral populations, chytridiomycosis was found. Our data suggest that the invasion of L. catesbeianus in Uruguay is at an early stage, with very localized populations, which might allow for the implementation of cost-effective management plans, with eradication constituting a plausible option.
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bullfrog Lithobates catesbeianus invasion in uruguay
Biological Invasions, 2008Co-Authors: Gabriel Laufer, Andres Canavero, Diego Nunez, Raul ManeyroAbstract:This is the first report of North American bullfrogs, Lithobates catesbeianus (=Rana catesbeiana), invasion in Uruguay. This Anura was introduced for farming proposes in 1987, but at present most of the farms are closed. At one of these closed farms, located at Rincon de Pando, Canelones, we report the occurrence of a feral population of L. catesbeianus. This invasion point is at an early stage and restricted to one or two ponds. We also report the effects of L. catesbeianus invasion in the community structure. This includes species composition and species size structure. In this system bullfrog tadpoles constitute a very important proportion of the present biomass. Bullfrog tadpoles appear to be displacing native amphibians and having some type of positive interaction with fishes. At the invaded system we found more fish species and larger sizes of the shared fish species. We analyze the involved risks of this invasion, the ecological impact by predation, the competition and habitat modification, and the potential of bullfrog to act as pathogens vector. We also recommend taking measures in order to avoid the expansion of this population. There is also the need of studies to search for new invasion points in Uruguay, especially where bullfrog farms were located.
Dario Elbio Cardozo - One of the best experts on this subject based on the ideXlab platform.
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american bullfrog Lithobates catesbeianus shaw 1802 invasion in argentina
Biological Invasions, 2010Co-Authors: Mauricio Sebastian Akmentins, Dario Elbio CardozoAbstract:The American bullfrog (Lithobates catesbeianus) has been introduced throughout the world over the past two centuries. This taxa is a potentially devastating invader because of its large size, prolific reproductive output, and broad ecological niche. Consequently, the extent of this ongoing biological invasion is an increasing conservation concern. In Argentina, several introduced populations have been reported. In most cases, these introductions have been intentional or incidental releases from breeding facilities, yet the consequences and effective controls for captive-breeding programs have not been assessed by government environmental agencies. Further studies are needed regarding the trophic ecology, reproductive biology, ecological niche, and chytrid fungus infection prevalence to predict the ultimate impacts of this species on native ecosystems. The aim of this work is to report a new alien population of L. catesbeianus at La Candelaria, Salta province, Argentina. This record represents the first population of American bullfrogs detected in northwestern Argentina.
Campos De Palmas - One of the best experts on this subject based on the ideXlab platform.
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widespread occurrence of the american bullfrog Lithobates catesbeianus shaw 1802 anura ranidae in brazil
South American Journal of Herpetology, 2011Co-Authors: Camila Both, Rodrigo Lingnau, A Lfredo, Bruno Madalozzo, Eoncio Pedrosa L Lima, Taran Grant, Francisco Beltrao, Campos De PalmasAbstract:We report new records of Lithobates catesbeianus feral populations in Brazil. Data were based on fieldwork, natural history collection records, and literature and electronic database searches. Lithobates catesbeianus occurs in 130 municipalities of Brazil, including 55 presented for the first time in this work. Most records are from south and southeastern Brazil in the Atlantic Forest biome with climatic conditions that are favorable to the establishment of bullfrog populations. The wide and possibly expanding distribution of feral L. catesbeianus populations in Brazil poses a major conservation challenge and demands research on the invasion patterns.
Michael J. Lannoo - One of the best experts on this subject based on the ideXlab platform.
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drought reduces chytrid fungus batrachochytrium dendrobatidis infection intensity and mortality but not prevalence in adult crawfish frogs Lithobates areolatus
Journal of Wildlife Diseases, 2014Co-Authors: Vanessa C K Terrell, Nathan J Engbrecht, Allan P Pessier, Michael J. LannooAbstract:Abstract To fully understand the impacts of the chytrid fungus Batrachochytrium dendrobatidis (Bd) on amphibians it is necessary to examine the interactions between populations and their environment. Ecologic variables can exacerbate or ameliorate Bd prevalence and infection intensity, factors that are positively related when Bd is acting on naive amphibian populations as an epidemic disease. In crawfish frogs (Lithobates areolatus), a North American species with a complex life history, we have shown that Bd acts as an endemic disease with impacts that vary seasonally; the highest infection prevalences and intensities and highest frog mortality occurred during late spring in postbreeding individuals. In this study, conducted between 28 February and 23 August 2011 in southwestern Indiana on the same population, we report an uncoupling of the previously observed relationship between Bd prevalence and intensity following an extreme drought. Specifically, there was a postdrought reduction in Bd infection inte...
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Population and Conservation Genetics of Crawfish Frogs, Lithobates areolatus, at Their Northeastern Range Limit
Journal of Herpetology, 2013Co-Authors: Schyler O. Nunziata, Stacey L Lance, Michael J. Lannoo, Joseph R. Robb, Daryl R. Karns, Stephen C. RichterAbstract:Abstract Crawfish Frogs (Lithobates areolatus) are a North American ranid, considered near threatened globally with populations in decline throughout their range. We studied populations of Crawfish Frogs on local and regional scales at their northeastern range limit to (1) assess the level of genetic diversity within populations, (2) estimate fine-scale genetic structure, and (3) estimate genetic differentiation between populations at the regional level. We used 10 microsatellite loci to genotype frogs collected from three regional sites in Indiana separated by 50–172 km and at one of these sites within a network of three breeding ponds
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strong site fidelity and a variety of imaging techniques reveal around the clock and extended activity patterns in crawfish frogs Lithobates areolatus
BioScience, 2010Co-Authors: Andrew S Hoffman, Joseph R. Robb, Daryl R. Karns, Jennifer L Heemeyer, Perry J Williams, Vanessa C Kinney, Nathan J Engbrecht, Michael J. LannooAbstract:Crawfish frogs (Lithobates areolatus) base their nonbreeding activities in and around the entrances of crayfish burrows. This site preference allows individual crawfish frogs to be monitored using still and video imaging techniques. We used three camera types offering different continuities, scales, and resolutions of data to observe the frogs' activity patterns and nonbreeding behaviors. Together, these techniques allowed us to observe two behaviors in crawfish frogs previously unreported for amphibians: (1) circumdiel activity patterns, and (2) long periods (days) of surface activity. Although these behavioral findings are at this time specific to crawfish frogs, we suspect that they may not be unusual activity patterns for other frogs, as well. The use of imaging techniques that take advantage of these frogs' dependence on burrows and use of burrow entrances has allowed us to observe these patterns for the first time.