The Experts below are selected from a list of 279 Experts worldwide ranked by ideXlab platform
Karine Laroucau - One of the best experts on this subject based on the ideXlab platform.
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High-resolution melt PCR analysis for rapid identification of Chlamydia abortus Live Vaccine strain 1B among C. abortus strains and field isolates.
Journal of Microbiological Methods, 2012Co-Authors: Fabien Vorimore, Annie Rodolakis, Hermann Willems, Simone Magnino, Victoria I Siarkou, Noémie Cavanna, Nadia Vicari, Karine LaroucauAbstract:We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus Live Vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
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differential identification of chlamydophila abortus Live Vaccine strain 1b and c abortus field isolates by pcr rflp
Vaccine, 2010Co-Authors: Karine Laroucau, Evangelia Vretou, Annie Rodolakis, Fabien Vorimore, Hermann Willems, Simone Magnino, Konrad Sachse, Victoria I Siarkou, Patrik M BavoilAbstract:Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydophila abortus and its nitrosoguanidine-induced, temperature-sensitive and virulence-attenuated Live Vaccine derivative identified point mutations unique to the mutant (Burall et al. [1]). Here, we evaluate the capacity of some of these mutations to either create or eliminate restriction sites using the wild-type strain C. abortus S26/3 as a reference. Three of eight genomic sites with confirmed point mutations (CAB153, CAB636 and CAB648) were retained for analysis as each resulted in the loss of a restriction site in the genome sequence of the Vaccine strain. PCR-restriction fragment length polymorphism analysis using restriction enzymes chosen to specifically target the three genomic sites was then applied to a large number of C. abortus field isolates and reference strains. Our results indicate that the three mutations are uniquely present in the Vaccine strain, and as such provide easy-to-use markers for the differential identification of the Vaccine strain and wild-type isolates.
Patrik M Bavoil - One of the best experts on this subject based on the ideXlab platform.
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differential identification of chlamydophila abortus Live Vaccine strain 1b and c abortus field isolates by pcr rflp
Vaccine, 2010Co-Authors: Karine Laroucau, Evangelia Vretou, Annie Rodolakis, Fabien Vorimore, Hermann Willems, Simone Magnino, Konrad Sachse, Victoria I Siarkou, Patrik M BavoilAbstract:Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydophila abortus and its nitrosoguanidine-induced, temperature-sensitive and virulence-attenuated Live Vaccine derivative identified point mutations unique to the mutant (Burall et al. [1]). Here, we evaluate the capacity of some of these mutations to either create or eliminate restriction sites using the wild-type strain C. abortus S26/3 as a reference. Three of eight genomic sites with confirmed point mutations (CAB153, CAB636 and CAB648) were retained for analysis as each resulted in the loss of a restriction site in the genome sequence of the Vaccine strain. PCR-restriction fragment length polymorphism analysis using restriction enzymes chosen to specifically target the three genomic sites was then applied to a large number of C. abortus field isolates and reference strains. Our results indicate that the three mutations are uniquely present in the Vaccine strain, and as such provide easy-to-use markers for the differential identification of the Vaccine strain and wild-type isolates.
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Genomic Analysis of an Attenuated Chlamydia abortus Live Vaccine Strain Reveals Defects in Central Metabolism and Surface Proteins
Infection and Immunity, 2009Co-Authors: Ls Burall, Annie Rodolakis, Abdessalem Rekiki, Gsa Myers, Patrik M BavoilAbstract:Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydia abortus and its nitrosoguanidine-induced, temperature-sensitive, virulence-attenuated Live Vaccine derivative identified 22 single nucleotide polymorphisms unique to the mutant, including nine nonsynonymous mutations, one leading to a truncation of pmpG, which encodes a polymorphic membrane protein, and two intergenic mutations potentially affecting promoter sequences. Other nonsynonymous mutations mapped to a pmpG pseudogene and to predicted coding sequences encoding a putative lipoprotein, a sigma-54-dependent response regulator, a PhoH-like protein, a putative export protein, two tRNA synthetases, and a putative serine hydroxymethyltransferase. One of the intergenic mutations putatively affects transcription of two divergent genes encoding pyruvate kinase and a putative SOS response nuclease, respectively. These observations suggest that the temperature-sensitive phenotype and associated virulence attenuation of the Vaccine strain result from disrupted metabolic activity due to altered pyruvate kinase expression and/or alteration in the function of one or more membrane proteins, most notably PmpG and a putative lipoprotein.
Annie Rodolakis - One of the best experts on this subject based on the ideXlab platform.
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High-resolution melt PCR analysis for rapid identification of Chlamydia abortus Live Vaccine strain 1B among C. abortus strains and field isolates.
Journal of Microbiological Methods, 2012Co-Authors: Fabien Vorimore, Annie Rodolakis, Hermann Willems, Simone Magnino, Victoria I Siarkou, Noémie Cavanna, Nadia Vicari, Karine LaroucauAbstract:We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus Live Vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
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differential identification of chlamydophila abortus Live Vaccine strain 1b and c abortus field isolates by pcr rflp
Vaccine, 2010Co-Authors: Karine Laroucau, Evangelia Vretou, Annie Rodolakis, Fabien Vorimore, Hermann Willems, Simone Magnino, Konrad Sachse, Victoria I Siarkou, Patrik M BavoilAbstract:Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydophila abortus and its nitrosoguanidine-induced, temperature-sensitive and virulence-attenuated Live Vaccine derivative identified point mutations unique to the mutant (Burall et al. [1]). Here, we evaluate the capacity of some of these mutations to either create or eliminate restriction sites using the wild-type strain C. abortus S26/3 as a reference. Three of eight genomic sites with confirmed point mutations (CAB153, CAB636 and CAB648) were retained for analysis as each resulted in the loss of a restriction site in the genome sequence of the Vaccine strain. PCR-restriction fragment length polymorphism analysis using restriction enzymes chosen to specifically target the three genomic sites was then applied to a large number of C. abortus field isolates and reference strains. Our results indicate that the three mutations are uniquely present in the Vaccine strain, and as such provide easy-to-use markers for the differential identification of the Vaccine strain and wild-type isolates.
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Genomic Analysis of an Attenuated Chlamydia abortus Live Vaccine Strain Reveals Defects in Central Metabolism and Surface Proteins
Infection and Immunity, 2009Co-Authors: Ls Burall, Annie Rodolakis, Abdessalem Rekiki, Gsa Myers, Patrik M BavoilAbstract:Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydia abortus and its nitrosoguanidine-induced, temperature-sensitive, virulence-attenuated Live Vaccine derivative identified 22 single nucleotide polymorphisms unique to the mutant, including nine nonsynonymous mutations, one leading to a truncation of pmpG, which encodes a polymorphic membrane protein, and two intergenic mutations potentially affecting promoter sequences. Other nonsynonymous mutations mapped to a pmpG pseudogene and to predicted coding sequences encoding a putative lipoprotein, a sigma-54-dependent response regulator, a PhoH-like protein, a putative export protein, two tRNA synthetases, and a putative serine hydroxymethyltransferase. One of the intergenic mutations putatively affects transcription of two divergent genes encoding pyruvate kinase and a putative SOS response nuclease, respectively. These observations suggest that the temperature-sensitive phenotype and associated virulence attenuation of the Vaccine strain result from disrupted metabolic activity due to altered pyruvate kinase expression and/or alteration in the function of one or more membrane proteins, most notably PmpG and a putative lipoprotein.
Wang Yun-xia - One of the best experts on this subject based on the ideXlab platform.
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Safety and Immunogenicity Testing of Monkey in the Preparation of Hepatitis A(Live) Vaccine,Freeze-dried by Cell Factory
Preventive Medicine Tribune, 2012Co-Authors: Wang Yun-xiaAbstract:[Objective]To compare safety and immunogenicity of rhesus after vaccination of Hepatitis A(Live) Vaccine,freeze-dried manufactured by cell factories and roller bottles process.[Methods]Vaccine should be injected by intravenous injection,observe the clinical symptoms,signs,biochemical,immunological reactions and pathological changes of Liver tissues of rhesus.[Results]No abnormal clinical signs were observed from both groups,ALT,anti-HAV antibody were persistently positive during the observation period,no viral hepatitis pathological changes on Liver tissues.[Conclusion]The immune effect of manufacturing process of cell factories and roller bottles of Hepatitis A(Live) Vaccine,freeze-dried are the same,they all have good protective effect from Hepatitis A.
Fabien Vorimore - One of the best experts on this subject based on the ideXlab platform.
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High-resolution melt PCR analysis for rapid identification of Chlamydia abortus Live Vaccine strain 1B among C. abortus strains and field isolates.
Journal of Microbiological Methods, 2012Co-Authors: Fabien Vorimore, Annie Rodolakis, Hermann Willems, Simone Magnino, Victoria I Siarkou, Noémie Cavanna, Nadia Vicari, Karine LaroucauAbstract:We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus Live Vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
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differential identification of chlamydophila abortus Live Vaccine strain 1b and c abortus field isolates by pcr rflp
Vaccine, 2010Co-Authors: Karine Laroucau, Evangelia Vretou, Annie Rodolakis, Fabien Vorimore, Hermann Willems, Simone Magnino, Konrad Sachse, Victoria I Siarkou, Patrik M BavoilAbstract:Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydophila abortus and its nitrosoguanidine-induced, temperature-sensitive and virulence-attenuated Live Vaccine derivative identified point mutations unique to the mutant (Burall et al. [1]). Here, we evaluate the capacity of some of these mutations to either create or eliminate restriction sites using the wild-type strain C. abortus S26/3 as a reference. Three of eight genomic sites with confirmed point mutations (CAB153, CAB636 and CAB648) were retained for analysis as each resulted in the loss of a restriction site in the genome sequence of the Vaccine strain. PCR-restriction fragment length polymorphism analysis using restriction enzymes chosen to specifically target the three genomic sites was then applied to a large number of C. abortus field isolates and reference strains. Our results indicate that the three mutations are uniquely present in the Vaccine strain, and as such provide easy-to-use markers for the differential identification of the Vaccine strain and wild-type isolates.